Document VG5oERZ7YxBY41N0Gz28pDraj
ro Olay 202 3%i2iem From-Charles River Labs Arkus Div.
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HYorosshaimn,By 108 10 Teter 215) 443.0587
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1096 ARGU;I S RESR EARC!H
Charles River Laboratories Discovery and Development Services
From: Raymond G. York, Ph.D., DABT=e
--
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To: John Butenhoff, Ph.D., DABT
ComPphaonnye:: 3(6M51C)o7r3po7r-a1t9e62Toxicology
Fax: (651)733-1773
Date: 01May 2002
Total Pages: 22
i
RE: Protocol 418-020
iAttached is the information on covariant analysis for study 418-020.
analyses were done for females and two for males.
So =i 3S3R zpg2:8
= = Four different
Females: 1. Sexual
maturation
w/
gestational
age
at
sexual
maturation
as
covariate
5
2. Sexual maturation w/ body weight at maturation as covariate
:
23.. EEssttrroouuss ssttaaggeess ww// dgeasyta6t4iopnoasltawgeaenaitnsgtabrotdoyf wesetirgohutsatsesctoivnagriaastecovariate I 3 5&2
Males:
. F285
21.." SSeexxuuaall mmaattuurraattiioonn ww// bgeosdtyatwieoingalhtaagtemaattusreaxtuiaolnmaastucroavtairoinataes covariate 2=S
We are mating
defining in the P
"gestational age" in this context as generation until evidence of sexual
the time in maturation
days from evidence of in the F1 generation.
Tdehleivreeraesdoanfitnegr 2is3rdatasysnaotfurgaelsltyadteiloinvetreyndafttoerw2e1igtho m2o3rdeayasndofagpepsetaartitoon.dePvuelposptfhaasttaerre
tthheanfiprsutppsuopfwtohuelsdabmee2s3egxeasfttaetrio2n1aldadyasysofolgdesatnatdiotnh.e
Both pups would be 1 day old second pup only 21 gestation
but
dmaoyrse oplud.psIfwaerteestdesluibvserteadnceearslhyo(rrteednuecdedprpeagrtnuarnitciyon(rleendgutche)dinleonngethgorfougpe,sttahteiopnuoprs ifmay
reach sexual maturation a day gestation or parturition length.
oItrwtowuoldbeafporpeeaprupthsatinthaegtreosutpsuwbhsitcahnhcaedwaaslocnaguesring
a
delay in the time
sexual maturation, spent in utero. By
when using
it actually affected the matemal animal by each pup's age from time of conception to
changing sexual
maturation as the covariant, the length of the stay in utero Is factored out.
M59 263
4
DieMar-200121102A:2G7oIm, SISFVr1omA-CAharias RivAaer LaVbEsDArUguIs EDivSu TENE bar Ssups SernOsTtr +po Feat patency of F1 generation male and female rats, respectively, using gestational age at sraetxsu)aolrmdaatuyroiftyvaagnidnatlhepabtoednycywe(ifgehmtalreecroatrsd)eadsotnhethceovdaaryiaotfesp.reputial separation (male `The Analysis of Covariance was also used to evaluate estrous cyciing of F1 generation bfeomdaylweeriagths,t uassinthgegecsotvaatriioantaesl.age at the start of estrous testing and day 64 postweaning hPelseiatsaeterteoviceownttahcetamtet.ached information and ifyou have any questions, please do not
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