Document VG5Mkm05nX18mnVXN3Q44xBBK

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium saltC,AS # 2795-39-3) Remarks: The 3M productionlotnumber was notnoted. The test sample isFC-600. Currentinformationindicatesitisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultonefoamer,1.00% sodium decylsulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyhle)xaminium chloride0,.80% starch,and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the test sample. METHOD: Method: Not noted. Test type:Acute static GLP: No Year Completed: 1975 Species:Pimephalespromelas Supplier:Not noted Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: LC50valuesgraphed. 95% confidenceintervals calculatedby probitanalysis. Test fishage: Not noted Average Length and weight: Length = 2.0 inches Weight = 1.5grams Loading: Not noted Pretreatment:Not noted. Test conditions: Dilutionwater: Carbon-filterewdater,Cityof St.Paul,MN. Dilutionwater chemistry: Not noted Lighting:Not noted Stock and testsolutionpreparation:Test solutionswere createdby directweights additions. Concentrations dosing rate:Once Stabilityof the testchemical solution:Not noted Exposure vessels: Not noted. Number of replicates:One Number offishperreplicat1e0: Number ofconcentration5sp:lusa blankcontrol Water chemistryduringthestudy: pH range(0-96hours): 7.0- 7.2(contreoxlposure) 7.3- 7.4 (2,000mg/L exposure)* Temperature range (0-96hours): 720F Dissolved oxygen range (0-96hours): 5.7- 7.0 mg/L (controelxposure) 4.0- 5.7 mg/L (2,000mg/L exposure)* Values forthe 2,000 mg/L exposure (thirhdighestconcentration) were used because totalmortalitoyccurredinthe highest concentrationstested. RESULTS Nominal concentrations: Bk control,1,000,1,500,2,000,3,000 and 4,000 mg/L Element value: 96-hour LC5c)=1,700 (1,465- 1,972)mg/L Element value based on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The value reportedappliestothatmixtureand notthe fluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hourLC50 forPimephales promelas was determined tobe 1,700 mg/L witha 95% Confidence Intervaolf 1,465to 1,972 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking3. The studylacksproperdocumentation of the method followed.The sample puritywas not properlycharacterized and thestudy lacksanalyticaclonfirmationofthe amount offluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, 1975. OTHER Last changed: 6/28/00 740101 EWIRONMENTAL ENGINEERING LABORATORY AQLJATIC TOXICITY MWK SHEET Type Test Test Organi Date Start Tine Started Material Tested Fe -*I) Avg. Noijlht Avg. Size Date Cavloted - Plotted LCsn -ToI(A 7.r Dilution Water IA eo,@a le, 01-51 Conc. Control 24 hr3 % Survival loa D.0 PH ppe 48 hrs D.0 Survival oH ppe 72 hrs % Survival 7z,-7o.T100-7-,----/00 D.0 9H ppo % hr3 % D.0 Survival- pH ppa -7.-a 7.05@-7 lob too -?2 /00 7 /00 7.3 1,@5 IGO 00 7, .90 60 7,3 :@AOOO loc> 7.q @;.-I z --3 s-o ZO 7,,@ 5. 4-oooi -.1 Averaze TomDerature Coments: 16titzr-16 /0 100 :)4 -3z 4 E -64 igrt- 4&eal".rLCso Calculation 7-3 4.-71--40 7, -@ 4; 0 7,3 I'd - -T- -77 0 7- 10 0 10 20 30 40 so 60 70 so go 100 Survival ENVIRONMERTAL ENGINEERING LABORATDRY AQUATIC MXICITY - PROBIT ANALYSIS WORK SHEET Date Started: Time Started: :Z,)5-7j- Material Tested: 6tv C)p -(AM (PM) Test Organism (Fathead minnow) Type Test: (Static) Avg. Wt._@.5 (gram) Avg. Size (inch) Exposure Period: ?0"--Hour Date Completed: 113-, -.@t3--1157 Diluent:_Ccarbon-filt.-St.PC.ity Wtr.) Analysis by: @MCLTO-L)@ (1) K No. of Doses Plotted Conc. Observed Mortality (2) Expected Mortality (3) (Z:J- 0 -E Corrected Observed- Va-lues -Expected Contribution to chi(from Nomoiraph #1) 0 17 2 3 4 _,Iooo /oo //.9 ..230 6 7 K Animals/Dose 40 Total Animals 2 chi (P-.05)for (K-2) S. @, heterogenous. is less io) Total contribution to chi (chi2)-contrib. to chi x Tot. Animals than degree of freedom (fromTable-2) therefore, the data are not a S6i) significantly 95% Confidence Limits for LC LC 84 Slope function (S) LC so 84//-LLCcSO + LC so/LC 16 LC 16 C, @-iooA-zrL> 2 Total number of animals used between 16% and 84% expected effects (N')--4o 2.77/A' fLcso - (S) -Cfrom Nomograph #2) LCso/fLCso - lower limit U0 LC so X flcso - upper limit x LC so (95% confidence limits t0 (1) Do not list more than two consecutive 0% effects or more than two consecutive 100% effects. (2) Expected value for any dose should be greater than 0.01% or less than 99.99%. (3) Corrected value for each 0% or 100% effect (from Table 1). There is a 95% chance that the true value of LC lies within these limits. so 99.8 99.5 95 70 so 540 30 20 10 .2 1 0 ------ IC7 41"1 C) o a 7f AW @\1 ft6 kt 17 1