Document V703Y5JD2n91k4b3rzad6BQj

B) AR226-2951 DU FONT USE ONLY Du Font HLR 301-90 ! Study Title STATIC AdiTB 96-BOUR LC50 OP TO FATHEAD MINNOWS, PIMEPHALES PRO Data Requirement United State^ Environmental Protection Agency Environmental Effects Test Guidelines Subdivision EG-9 Author Kevin M. Baer, Ph.D Study Completed On July 17, 1990 Performing Laboratory Baskell E. I. du Font de Nemours and Company, Inc. Laboratory for Toxicology and Industrial Medic; Elkton 'Road, Post Office Box 50 Newark, Delaware 19714 Laboratory Project ID Baskell Laboratory Report No. 301-90 Page 1 of 12 Company Sanitized. Does not contain TSCA CBS '^pRptp^ ^'n^i Du Font BUl 301-90 GOOD LABORATORY PRACTICE ll^^ioj^ ^1 This study was conducted according to the IT, S. Environmental Protection Agency's Good Laboratory Practice CRF 792) for TSCA. Areas of non-compliance are docunente1jd in the ?t*idy 'ffswt^B- No deviations existed that significantly affected the validity of the itudy. Spon-pr: Subnitter; B. I. du Pent de Neinours and Co., Inc. Cheoicals and Pigments Department Study Director: /fS^. M fcM^ Kevin N. Baer, Ph.D. Research Toxicologlst Environnental Toxicology and Info mat ion Servi ces ^-15-^ Ho/Day/Yr Company Sanitized. Does not contain TSCiB CRF Du Font HLR 301-90 QUAlllTAYSSURANCE DOCUMENTATION STUDY: H 18,267 TOSTC<AT|lHIECAACDUMTEINN9O6-WHSO,URPIMLECP5H0 ^LOEFS|_PR_O_M_ELAS AUDITSs Items Audited Protocol, Conduct Records, Report Audit Dates 5/14/90 6/21/90; 7/3,5/90 B ^ H DSHAOTERTF-TINERDMINGASUDRITEPORERPTOEDRTTONUMMBAENRA;GEMENTAND STUDY DIRECTOR: I 7/5/90 ~y--^ Reported by; 7^ William J. Lynam Quality Assurance Auditor ?//^^ Date 3 - Cofftpany Sanitized. Does Tiot conlalnTSCACBI I Du Font BLR 301-S j 1 TABLE OP COBTBffS ! Page Good Laboratory Practice Statement 2 ................. Quality Assurance Documentation 3 i ................... Table of Contents 4 i .......................... I. Purpose 5 .............................. II. Introduction/Summary 5 ...................... Records-;.-.-;-.. . :. 5 General Information 6 ............................................ III. Materials an.: Methods - 7 A. Test Material 7 ..................... B. Dilution Water 7 ........................ C. Test Fish 7 ....................... D. Test Methods 7 .......................... B. Chemical Analysis 8 .............................................. IV. Results and Discussion 8 ..................... V. Conclusion B ........................... VI. Literature Cited 8 ........................ VII. Tables 9 Table 1. Chemical Characteristics of Baskell Laboratory Veil ............................. Water 9 Table 2. ......................... Table 3. Water Chemistry of the Water Control at Test Start Physical and'Chemical Characteristics of^----fl Test Solutions 10 . . 11 .................... Table 4, W---- Observed Mortality in Percent of Fathead Minnows, Piaephales proaelas, Exposed to for 96 Hours in a Static, Unaerated Test . . . . . . . 12 Company Sanitized. Doas no! contain TSCA CB1 Du Font HLR 301-90 I. PURPOSE The objective was to assess the acute toxicity of minnows, Pimephales proaelas. to fathead II. IRTBODOCTION/SUM(ABT __ was not acutely toxic to fathead minnows, Pinephales promelas, in an unaerated, static 96 hour test.. Nominal concentrations vere 313, 625, 1250, 2500 and 5000 ag/L. The 96-hour LC50 could not be calculated because 5000 og/L, the highest nominal! concentration tested, failed to cause nortality of the fish. ! RECORDS All data and the final rjeport will be maintained either at Haskell Laboratory for Toxicology and' Industrial Medicine (Newark, Delaware) or be archived at the Du Font Records Management Center (Vilmington, Delaware). Technician: ^ -SL^- \l.f^^- R. Scott Vivian Technician,Leader Environmental Toxicology and Information Services Study Director: Kevin N. B^erTPh.D. Research Toxicologist Environmental Toxicology and Information Services <.-^-9& Ho/Day/Yr ^-/S-?0 Mo/Day/Yr THIS REPORT IS APPROVED FOR ISSUE. y^^ aJ^ Kelvin N. B^er, Ph.D. Study Direcfctor 7//7/?ff Mb/Ddy/Yr ^ Company Sanilized. Does nol conlain TSCA CBl ^.:. ^ ^ ^ Q ^ "'^^l;i^a--33:s;:.i?:t^;Sje(alt^^a.w.,.^. . -; ;7 ''i-1:*''-1 '-.. -..'.-. ''-i'^^' ""i'" '"'-'flISI Du Font HUl 301-90 Material Tested: Medical Research Number: Baskell Number! Synonyms: Purltys Composition: Sponsor: Submitted by: Study Schedule: Notebooks: CheBilisals and I. E. du Font Jackson Lab 3P.lspents Department 4i6'l^"o"rs and Co., Inc. Chemicals anti I. E. du Ppn't Jackson Lab Pi^ients Department de'"iSemours and Co. 9 Inc. Study Initiation Date: 5/11/90 Experimental Start Date; 5/14/90 Experimental Completion Date: 5/18/90 Study Cempletion'Date: 7/17/90 Haskell Laboratory Test Code: Distribution: Company Sanitised. Does not contain TSCA CPi Cj Font HLR 301-90 in MATERIALS AND METHODS A. Test Material Source and purity offllplB|are described under General Information. The appropriate quantity oTfl|----B vas measured and dispersed into 4 liters of dilution water (see below)'lii the test container In the water bath. Each tank was vigorously stirred p^ior to addition of the test animals. Stability and scfotlliuiMbi1liI t-yv nof ---------- Iinnldn<ilIunt(i"oInnn uwaatt-ear vwoerae nnot determined. B. Dilution water ! Water (Table 1) originated from the Haskell Laboratory well. C. Test Pish ; . . Fathead minnows tPimepha: es pronelas) were reared in continuously flowing well water at Haskell Laboratory. Adult fish resided in 38 L aquaria at approximately 25C and were fed frozen brine shrimp (Artcnia so., San Francisco Bay Brand, Newark, CA) generally twice dailyi once on Saturdays and not on Sundays. Eggs were deposited on polyvinyl chloride tiles coated with sand. Tiles with eggs were aerated and incubated at 25C in a water bath. Larvae were reared in 22 L aqujaria at approximately 22 C and were fed newly hatched Artemia generally twicje daily on weekdays and daily on weekends. Juveniles were held in 22 L aquaria at approximately 21C and were fed newly hatched Artemia and/or frozen l)rine shrimp generally twice daily on weekdays, once on Saturdays and not on Sundays. No treatment of the fish for diseases was required during the holding; period. Sickness, Injury or abnormality were not present. Animals were ideitifled by labels on aquaria and test vessels. D. Test Methods [1] Five test concentrations and a water control were used. Nominal test concentrations were 313, 625, 1250, 2500 and 5000 mg/L. Fathead minnows of approximate age 88 days, mean JLength2.3 era and mean weight 0.149 g were used. Observations were made twice d&ily. Glass aquaria (5 L) containing 4 L of test solution (12-cm depth) were employed. Two replicates of e^ch test concentration with ten fish in each replicate were used. Fish wer^ added to test aquaria using random numbers and were not fed for approximately^48 hours prior to the test nor during the test. Mean loading was 0.37 g/L. Te^t solutions were held between 21.9 and 22.7-C (mean 22.3-C) and were unaerat^d. A photoperiod of 16 hours light (237 to 269 Lux) versus 8 hours darkness vas employed. Cofflpafty Sam^zed. Does nol conlamTSCACBl Du Font BLR 301-90 Dissolved oxygen and pd were measured In both replicates of the water control and test concentrations at the beginning of the test (before the addition of fish) and daily Ithereafter. Total alkalinity, EDTA hardness and conductivity were measured in an analogous water control sample at test ' start. i- E. Cheatcal Analysis : No chemical analyses of the test solutions vere performed. IV. RESULTS MB) DISCUSSION The dissolved oxygen (lj).0.) values in the 5000 ng/L test concentration dropped to below 60X of saturation at 96 hours. This apparent decrease in D.O. was not biologically significant based on the lack of observed mortality at that test cone'-(ration.: All other chemical and physical parameters (Tables 2 and 3) vere within expected ranges. All test solutions (excluding controls) exhibited a transparent white color. The 96-hour LC50 based on observed mortality (Table 4p and nominal concentrations offH--B--l could not be calculated because 5000 ftg/L failed to kill any of the fish. V. CONCLUSION fUB minnow test. concentration was not acute]ly toxic in the static, The LC50 was greater than 5000 mg/L, tested. unaerated 96-hour fathead the highest nominal VI. LITERATURE CITED 1. United States Environmental Protection Agency, Office of Toxic Substances, Guidelines for Testing Chemicals, EG-9 totti^anySamllzed. Does r\o\ contain TSCA CB^ Du Font HLR 301-90 TABLE 1 Chemical Characterijstlcs of Baskell Laboratory Veil Water1 Paraaeter Concentration Pararnei r Concentration BOD, Bg/L COD, Bg/L DOC, ng/L TOC2, Bg/L Kjeldahl N, mg/L Ammonia H, mg/L Organic N, ng/L Turbidity, NTU Phenolics, ng/L Color, Co/Pt Solids total suspended, Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, Bg/L Boron, mg/L Cadmium, mg/L Calcium, mg/L Chloride3, mg/L Chromium, og/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron3, mg/L ng/L 51 <ld <t.O 0.8 <0.2 <Q.2 <0.2 <lj.O <Q.05 <5 : <4 <Q.100 <0j.060 <0!.010 <a.001 <Q.021 <0!.002 25!. 4 61.3 <Q.010 <0i.020 <0'.010 <0i.025 0.131 Pluoride3, Bg/L Lead, Bg/L Hagnesium, ng/L MBAS/LAS. Bg/L Mercury, ng/L Nickel, ng/L Nitrite3, Bg/L Nitrate', Bg/L Phosphate3, ng/L Potassium, og/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, Bg/L Zinc, mg/L Volatile priority pollutants Acid extractable priority pollutants Base/neutral priority pollutants Pestlcides/PCBs Organophosphate pesticides4, ug/L <0.1 <0.^75 3.9 <0.05 <0.00020 <0.020 <0.1 1.7 <0.1 1.8 <0.005 <0.010 B.I 5.0 0.11 <0.020 MD5 HD6 ND6 ND6 <0.50 ^ate of analyses 24 October 1989 unless indicated otherwise. 2 Date of analyses 5 December 1989. 3 Date of analyses 11 December 1989. ^Date of analyses 26 September 1989. 'None detected except methylene chloride was below the published Method Detection Limit (BMDL). SNone detected. CoTnpahy Sariffized. Does hoi cohlainT'SCA CBI 10 Company Sanitized. Does not cortiamTSCA CBl ' .' ; . i : ; ::-rtfss,^ ' .' '-' 's'" <s ' I ] 1 il ^ 11,) ^l.\ Du Font HLR 301-90 TABLE 3 Physical and Chemical Characteristics offJ^Test Solutions Tine Water Control Ai V 313 A* Bi NoBinal Concentrations (W.) 625 A* Bi 1250 A V- 2500 A1 V 5000 A^----P ! Dissolved oxygen* (Bg/L) ! ', Start 8.4 8.4 8.5 8.5; 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 24 HR 48 HR 7.1 7.2 7.1 7.1 7.1 7.2; 1 7.1 7.1: 7.3 7.4 7.2 7.1 7.6 7.6 7.2 7.2 7.6 7.6 6.8 6.7 7.4 7.3 6.8 6.8 72 HR 7.0 7.0 7.0 7.0; 7.0 6.9 6.1 6.1 5.5 5.5 5.8 5.8 96 HR 7.3 7.4 7.6 7.6; 7.6 7.5 6.3 6,2 5.9 6.0 4.9 4.7 pHh Start 24 HR 48 HR 72 HR 96 HR 7.2 7.2 7.3 7.3 6.7 6.7 7.2 7.2 7.5 7.5 7.2 7.2; 7.3 7.3 6.8 6.8; 7.3 7.3; 7.5 7.6; 7.2 7.2 7.3 7.3 6.8 6.8 7.2 7.2 7.6 7.6 7.2 7.2 7.3 7.3 6.9 6.8 7.2 7.2 7.5 7.5 7.2 7.2 7.3 7.3 6.8 6.8 7.2 7.2 7.5 7.5 7.2 7.3 7.3 7.3 6.8 6.8 7.1 7.1 7.5 7.5 lEac.h replicate contained 10'fish. YSI Model 54A, Yellow Springs Instruments, Yellow Springs, OH. !' '"Corning Model 610A, Corning;Scientific Instrunents, Medfield, KA. 11 Company Sanitized. Does not coritainTSCA CCT Du Font BLR 301-90 TABLE 4 Exposed to) for 96 Hours in a Sta tic, Unaerated Test Nominal Concentration (Bg/L) Percent CuBulative Observed Morfality 24 Hours Ai B^ 48 Hours A* Bi 72 Hours Ai Bi 96 Hours Al Bi Vater Control 313 625 1250 2500 5000 0 0^ 0 Oi 0 0; 0 0 0 0; 0 ! 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ^ach replicate contailied ten fish. 12 Company San'tfrsd. Docs r"^ contain TSCA CBS