Document V3yo5GaRNZ4XQM63YjEeY9aGo

# A* i V 2-< I V ?* * t'.Ly. E. I. du Pont de N emours & C ompany INCOHPOHM t W ilm in g t o n . D e l a w a r e 19898 P O L V M f R P R O D U C T S DC r / R T w C R T E X ^ L P I P C N I H I S TA T ION PERSONAL AND CONFIDENTIAL cc : A . J. Dahl B. W. Karrlt L. J. Papa Pra1 File 1 .C. 353 K1 1400 269 -A/S.ZZ-/S$S July 16, 1981 TO: DR. Y. L. POKER - P*P*D~,> P*-ark'-ewr*-sburg FROM: S. S. STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLU0R00CTAM0ATE (Job No. 810-578; PRAL Nos. 81-2218-2232,81-2600-2604,81-2758; ______Notebook Nos. E22514.E26238)_________________ As requested in your letters of 5/18 and 6/12/81 to L. J. Papa, the 21 blood samples submitted then have been analyzed for perfluorooctanoate (Cg>. Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of a, 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Word s: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC There s a world of Ihings we're doing something about 000096 EXP000035 EID713849 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sar.nle PRAL No. Date Sampled 81-2218 81-2219 31-2220 81-2221 31-2222 81-2223 81-2224 81-2225 81-2226 81-2227 81-2228 81-2229 81-2230 4/29/81 4/29/81 4/30/81 4/30/81 5/1/31 5/1/81 5/1/81 5/4/31 5/7/81 5/8/81 5/11/81 5/14/81 5/14/81 P.R.S 4354 4554 4380 4639 4335 4559 WS 4331 4 500 ws 4473 - 81-2231 81-2232 81-2600 81-2601 81-2602 5/14/81 4/30/81 5/19/81 5/19/81 5/26/81 1809 767 w. s . - 81-2603 81-2604 81-275B 5/28/81 6/1/81 6/19/81 3558 1830 - Name GC Analysis Date Analvred [Cr ], l!g f 6/12/81 6/12/81 6/12/81 6/12/81 6/12/81 6/12/81 6/11/81 6/11/81 6/11/81 6/11/81 6/8/81 6/8/81 6/8/81 f 6/9/81 S 6/8/81 6/8/81 6/17/81 6/17/81 6/15/81 0.034 0.009 0.76 0.098 0.64 0.031 0.17 3.9 4.8 0.045 0.042 0.095 0.25 0.31 1.3 2.1 0.019 0.012 6/17/81 6/17/81 6/26/81 0.057 0.75 0.095 (a) Analysis is described in Lab Method ES-567 ("Determination of Ferfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 yjgF/g. The detection limit is 't 0.004 igF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [C^j 0.004 ppm, which cannot be distinguished from reagent background. *R E D A C T E D * EXP000036 EID713850 000097