Document V3ddG7086pkBK1BmrKwajKoBq

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (EASTERN OYSTER) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95,oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFC-203. Currentinformatioinndicateistisa mixtureof1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol2,.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: Standard PracticeforConductingStaticBasicAcute Toxicity TestswithLarvae ofFour SpeciesofBivalveMollusks(ASTM, DraftNo. 5). Type: Acute static GLP: No Year completed: 1979 Species: Crassostreavirginica Supplier:Inducedspawninginthelaboratoroyffield-collecmtaetdureadults from an estuaryadjacenttoBiloxiM,ississippiM.aintainedatBMRL until testing. Analyticalmonitoring: Temperature,pH, salinitayn,d DO. Exposure period: 48-hours Test organism age: Empryos, within1 hour afterfertilization. Statisticamlethod: Testconcentrationcsonvertedto logarithmand correspondingpercentagereductionofnormal larvaewas convertedtoa probit.EC50 valuesthen calculatedusinglinearegression. Test conditions: Dilutionwater: Filterendaturalseawater pumped from Big Lagoon, a Gulfof Mexico estuaryadjacenttothe laboratoryP,ensacola,FL. Dilutionwater chemistry: Salinity2:2 ppt Lighting:Not given. Stock and testsolutionpreparation:A primarystocksolutiownas preparedby addinga weighed amount oftestsubstanceto filteresdeawater. Exposure concentrationwsere then preparedby additionoftheappropriate volume ofstocksolution. Exposure vessels: 1 L glassbeakerscontaining900 mL of testsolution. Number of replicates:3 Number of organisms per replicate:Approx.23,400 embryos Number ofconcentratiofnisv:pelusa blankcontrol Elementbasis: Numberofnormalldyevelopeldarvaec,ountewdith Sedgwick-Raftercell Water chemistry during the study: Temperature range (0-48hours): 20 1 *C (Temperaturecontrolled waterbath) Salinitryange (0-48hours):22 ppt pH range (0-48hours):8.0-8.1 Dissolvedoxygen range (0-48hours): > 67% saturation RESULTS Nominal concentrations:Bk control0,.6,1.0,3.2,5.6,and 10 mg/L. Element values: 48-hourEC50 = 3.5(0.5- 22)mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks fieldT.he valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsubstance48-hour EC50was determinedto be 3.5 mg/L witha 95% Confidence Intervaolf0.5to22 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However, sample puritywas notpropedycharacterizeadnd the study lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Test was conducted by EG&G BionomicsMarine Research Laboratory, Pensacola,FL atthe requestofthe3M Company, Lab Request number 4971 S, Sample 7902, 1979. OTHER Last changed: 6/27/00 Acute toxicity of 3M Company's Sample .7902 to embryos-larvae of eastern oysters (Crassostrea virginica) ,ee-zo3. 9,t/ -=Ar 7,yzgs LR dl971-S Toxicity Test Report Submitted to 3M to@npany St. Paul, Minnesota Project Number H90-500 Report Number BP-79-8-123- EG&G, Bionomics Marine Research Laboratory Route 6, Box 1002 Pensacola, Florida 32507 August 1979 A marine toxicity test was conducted at Bionomics Marine Res.earch,.Laboratory (BMRL),.Pensacola, Florida, to determine the effect of Sample 7902 on embryos-larvae of eastern. oysters (Crassostrea virginica). The criterion for.effect was reduction of the number of normal larvae (those which developed to the fully-shelled, straight-hinged veliger stage within 48 hours) in test concentrations as compared to the number of normal control larvae. Results of the test are expressed as aCCB-hou@r7:ECD5D(the concentration of Sample 7902*estimated to be effective in preventing normal development of 50% of the exposed eltbryos-larvae). Data from the test are maintained at BMRL. MATERIALS AND METHODS Test material The sample was received at BMRL on 3 July 1979, and was con- tained in a 500-milliliter (mt) NALGENEO bottle labeled "3M SAMPLE (:7:29p BIOASSAYS: 96hr LC50-GRASS SHRIMP"(PALAEMONETES VULGARIS); 48hr LC50-ATLANTIC OYSTER LARVAE (CRASSOSTREA VIRGINICA)." The sample was a medium orange liquid. Concentrations are reported here as milligrams (mg) of whole test material pet I of seawater or as parts per millioll (ppm). Test animals Oyster embryos were obtained by induced spawning of sexually mature adult oysters which had been collected from an estuary adjacent to Biloxi, Mississippi, on 20 July 1979 and maintained in flowing, unfiltered seawater at BMRL until testing began. 2 Test water Water used for spawning and testing was natural seawater which was pumped from Big Lagoon, a Gulf of Mexico estuary adjacent to BMRL. The pump intake was about 85 meters of approximately 3 m... offshore at a depth'. Seawater was pumped by a #316 stainless steel pump through hard polyvinylchloride (PVC) pipes, through sandfilled fiberglass filters, and through 10-micrometers tim) pore size polypropylene core filters into an elevated fiberglass resex-voi*r. Wate3@ was continuously and vigorously aerated.in the reservoir and flowed by gravity through PVC pipes into the laboratory. . There it was pumped through a 5-pm pore size polypropylene core filter and distributed into test chambers. The chemical composition in Appendix A. of BMRL seawater is characterized Test conditions kiethods for the 48-hoiir oyster embryo-larvae test were based.on Standard Practice for Conducting Static Basic Acute Toxici@-y" Tests with Larvae of Four Species of Bivalve Molluscs (ASTM, Draft No. 5) Individual, sexually mature female oysters were induced to spawn by placing them in glass chahibers -containing 1 t of f iltbred (5-pm), 26 degrees Celsius (OC) seawater and increasing the water temperature to 320C in the presence of viable sperm excised from the gonad of a sexually mature male oyster. Fertilization occurred upon release- of the eggs into the spawning chambers and was confirmed microscopically. Fertilization success was estimated to be 190%. Density of the embryos was determined by a Sedgwick-Rafter count of a 1:10 dilution (1 mt embryo susr)ension:9 mt se*awater) from the svawning chamber. 3 All concentrations and the control were triplicated. Test containers were 1-.tglass beakers, each of which contained 900 mt of filtered (5-pm), natural seawater. A primary stock solution was prepared by adding a weighed amount of Sample 7902 to a known volume of filtered seawater and the appropriate volumes were added to each.-testcontainer to obtain the desired test concentrations. Each test container was inoculated with an estimated 23,400 embryos within 1 hour after fertilization and then maintained at 2010C in a temperature-controlled water bath. After- 48 hours of exposure, the larvae from each container were collected in a 37-vm mesh size sieve, rinsed into a plastic bottle with 24 mt of filtered seawater, and preserved with 1 mt of neutralized formalin. The number of normally developed 48-hour larvae was determined by a Sedgwick-Rafter count from each triplicate test-and control container. Percentage reduction of normal embryos was determined as follows: Percentage reduction Number of normal 48-hour'control larvae minus the number of normal 48-hour larvae in each test concentration Number of normal 48-hour control larvae x 100 The test was conducted 1-3 August 1979. Statistical analyses* Each test concentration was converted to a logarithm and the corresponding percentage reduction of normal larvae was con- verted to a probit (Finney, 1971). The 48-hour EC50 and 95% con- fidence limits were then calculated by linear regression. 4 RESULTS AND DISCUSSION The calculated 48-hour ECSO for embryos-larvae of eastern oysters exposed to Sample 7902 in static, unaerated seawater was 3.5 ppm with 95% confidence limits of 0.5-22 ppm Reduction of embryos-larvae which developed normally to the straight-hinged veliger stage after 48 hours was from 12% in 0.6 ppm to 74% in 10 ppm (Tables 1 and 2). Measured concentrations of dissolved oxygen remained 267% of saturation and the ph'was from 8.0-8.1 after 48 hours of exposure. 5 REFERENCES American Society for Testing and Matekials Committee E-35 on Pesticides. August 1978. Standard Practice for Conducting Static Basic Acute Toxicity Tests with Larvae of Four Species of Bivalve Molluscs. Draft No. 5. Finney, D.J. 1971. Probit Analysis. Cambridge University Press, London. 333 p. 6 TABLE 1. Toxicity of 3M Company's sample 7902 to embryoslarvae of eastern oysters (Crassostreacvirginica) exposed for 48 hours in static, unaerat seawater. The criterion for effect was the reduction of the number of normal larvae in test concentrations as compared to the number of normal control latvae. Salinity was 22 O/oo and temperature, 2010C., apercentage reduction Nominal concentration ,(Mg,/.t;pprt) Control 0.6 1.0 3.2 5.6 10 Percentage reduction of normal'48-hour larvaoci 12 31 44 61 74 Number of normal 48-hour contr ol larvae minus the number of normal 48-hour larvae in each test concentration Number of normal 48-hour control larvae x 100 7 TABLE 2. Calculated number of normal eastern oysters (Crassostrea virginica) larvae.following 48 hours of exposure to 3M Company's Sample 7902 in static, unaerated seawater. The numbers were based on Sedgwick-Rafter counts. initial inoculum was 23,400. salinity was 22 0/00 and temperature, 2010C. Nominal concentration (mg/y,;Ppm) Control 0.6 1.0 3.2 .5.6 10 Rep A 19,380 15,390 13,822 12,112 8,408 5,771 Number' of normal larvae Rep B Rep C Mean S-D-N-- 22,515 18,382 20,092 2,157 18,098 19g238 17g575 lv976 11,970 15,818 13,870 1,924 Si,978 l2y825 11,305 -2,046 6,484 8,764 7,885 1,226 3,919 6,,270 5,320 11239 astandard deviation. APPENDIX A Results of Chemical Analyses for Routine Characterization of Selected Chemical Constituents in Bionomics Marine Research -Laboratory Seawater Constituent ..Concentratioirl-@(mq/t;PPMT30 January 1979 14 June 1979 Arsenic Cadmium Chromium Copper Mercury Nickel Zinc Lead Total Phosphate as P Ammonia Nitrogen as N Nitrate Nitrogen as N Nitrite Nitrogen as N Total Petroleum Hydrocarbons. Sulfides .Pesticidei Polychlorinated Biphenyls <0.001 0.002 0.023 .0.0007 0.02 0.05 <0.001 <0.02 0.42 <0.01 <0.01 <5.0 <1.0 None detecteda None detecteab 0.006 <0.01 <0.01 <0.01 <0.0005 <0.01 <0.02 <0.02 <0.02 0-14 <0:01 <0.01 < 5. 0c; <1.0 None detected None detected- Water samples were collected from Bionomics Marine Research Laboratory seawater system after the mixing -station in the wet lab. a Pesticides: BHC, lindane, heptachlor, heptachior epgxide,. aldrin,dieldrin, endrin, perthane, DDE, -tDE (DDD), DDT, methoxychlor, endosulfan, strobane, toxaphene, kelthane, and chlordane all <0.005 bpolychlorinated Biphenyls: Aroclore 1016, 1232, 1248 1260, 1221, 1242, and 1254: all.<0.05 jig/t., Cpetroleum hydrocarbon sample collected 10 July 1979. Al PREPARED BY: AUDITED BY: REVIEWED BY: APPROVED BY: 8 Terry A. Hollister Study ;Yirectof G. Scott Ward Quality I ce Unit Peter Shqba, Ph.D. all T-r--O-32,eCcotordinator Rod Parrish Director