Document V3aRoj85kkkeR7vyOoVx5oX4g
Form 6747-1 1 A
TECHNICAL REPORT SUMMARY
Dete
8/4/78
TO: TECH N ICA L COMMUNICATIONS CENTER - 201-2CN
,, mportant - if report is printed on bod) sides o f paper, send two copies to TCC.)
(Division
CRL - Analytical and Properties Research Laboratory
Project
Trace Fluorochemicals in the Environment
Report Title
IRDC 137-092: FC-95/Monkey
To
J.E. Long
Jon Belisle
Notebook Reference
Dept. Number
0502
rro lect Number
A00006
Report Number
967
Em ployee flum berlei
88240 a tn o . r * g r In clu d in g C oversheet
SECURITY
G Open
KEYW O RDS: (Select terms from 3M Thesaurus. Suggest other
applicable terms.)
Q f Closed
CURRENT OBJECTIVE:
Requestor: J.E.
Long;
3M CHEMICAL ^ REGISTRY ^
Nave Chemicals Reported
Yes
J O No
Request #A69507; Project #9172110004
Central Research Analytical Report Zhemical/Analysii;
REPO RT A B ST R A C T: (200-250 words) This abstract information is distributed by the Technical Communications Center to alert 3M'ers to Company R&D. It is Company confidential material.
The level of FC-95 was determined In the serum and livers of monkeys from the IRDC subacute toxicity study 137-092.
''* motion Luiison
!mii.
M U 'U - L l" 0
CENTRAL ANALYTICAL LABORATORY
Report No. 6967 D ate_____A ugust- &+.J-9.18
Subject: IRDC 137-092 : FC-95/Monkey
R e q u e s t o r : _____. I . E . - L o t r g ------
R e q u e st No. . A 2 0 7 ................
D ept. N am e .J K U tiC O lO O T -
Dated . . . S / l / . V L ................ --
Report:
Proj. No.91J2LLH004
Reference 137-087 by IRDC was a 90 day subacute Rhesus monkey toxicity study of FC-95. Incorrect (too high) feeding levels were used and all animals died within the first few days. The feeding levels were lowered and the study started again as 137-092. The serum and livers were each individually submitted for analysis. Each group contained 4 monkeys, 2 male and 2 female as follows:
Group
I II III IV
Dosage Level 0
0.5 mg/kg/day 1 .5 mg/ k g / d a y 4.5 mg/kg/day
Survival 4/4 4/4 4/4 0/4
SERUM ANALYSIS
Monkey
Dosage Level
FC-95 in Serum (ppm)
Blank on Method
-
2
735 5M 735 8M 7368F
0 40 0 20 0 15
7463M 7466F
0.5 mg/kg/day
II
150 150
746 2M 7500F
1.5 mg/kg/day
tl
250 275
Our newly developed pyrolysis method was used. Precision is estimated to be 10 - 25%.
A.R. #6967
2 August 8, 1978
The hiah levels of FC-95 in the control serum cannot be explained.
For example, 7355M was run on 2 different days with the same experimental
results. Perhaps a total fluorine should be run on this sample to
determine if this level of F is present. See Table below containing
liver results for further discussion (Footnote 2) .
-
LIVER ANALYSIS
Monxey
Dosage Level
7355M
0
7368F
0
FC-95 in Liver(mg) 3000 1500
FC-95 in Li 50 2cf
74 6 3M 7466F
0.5 mg/kg/day
II
7000 8000
100 100
7 46 2M 7500F
1.5 mg/kg/day
II
45000 40000
650 600
7484M 7S02F
4.5 mg/kg/day
II
40000 80000
650 1000
The livers were stored in the refrigerator several months prior to analysis resulting in the separation of some liquid. The high values and physical size necessitates taking only partial livers in which case the same ratio (estimated visually) of solid to separated liquid was taken for analysis. In addition, recovery of FC-95 was estimated from experiments where FC-95 was added to a control liver.
(D Because of a higher than expected value for the control, a 0.133 g liver sample was analyzed for total fluorine. The fluorine value calculated as FC-95 is equivalent to 11 ppm FC-95 in the liver. As time permits, more work should be done to verify these "high" control levels.
236-3
