Document V3Y6mn19gm6KxO9312eaJZBN8

PROPOSAL TO THE NEW YORK STATE HEALTH RESEARCH COUNCIL TITLE: The Environmental Health Implications of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) in the Hudson Estuary PRINCIPAL INVESTIGATOR: Dr. Chun C. Lee (SSN 521-52-6704) INSTITUTION: New York University Medical Center Institute of Environmental Medicine Laboratory for Environmental Studies 550 First Avenue New York, New York 10016 DURATION OF PROPOSED WORK: January 1, 1979 through December 31, 1979 TOTAL AMOUNT REQUESTED: $31,080 fchun chi Lee Research Scientist Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 l Associate Director Institute `of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 7>y 'r Norton Nelson, Director Institute of Environmental Medicine New York University Medical Center 550 First Avenue New York, New York 10016 ThoeasjU Fitzgerald, Director Office of Grsaes Administration sad Institottonal Studies 550 First Avenue New York, New York 10016 MQNS 207519 HEW YORK STATE HEALTH RESEARCH COUNCIL GRANT APPLICATION PRIORITY AREA # 7 t^T NAME _ Lee SECTION I SOCIAL SECURITY # 521-52-6704 .... Wk coMPIgi1^ ay principal iH'vESfffiATflrt--------------------------------- T. TITLE OF PROPOSAL The Environmental- 2. G DEPAaggsr, 57^77^, Health implications of Aerobic and Anf OR EQUIVALENT Lab. for Environments aerobic Oecomppsition of_Polychlorinaied Studies, Inst, of Environmental 2. PRINCIPAL INVESTIGATOR Biphenyls (PCBs) Medicine, NYU Medical Cem-r in the Hudson Estuarv_2. 1. major SUEPTYTSIOS T1 S'! NANS (Last, first, Initial) Medical Center "~ Lee, Chun C. 3. DATES OF PROPOSES PROJECT PERIOD (THIS T. r ortitle position Research Scientist and Adjunct Assistant Professor __________ 2. C. MAILING ADDRESS (Strut, City, P.O. Box 817 State, Zip Code) Tuxedo NY 10987 . APPLICAZICN) 4. TOTAL DOZCr COSTS FOR PERIOD a im 3 WWHH JAr 17 f 7 S. DIRECT COSTS REQUESTED FOR FIRST 12-MONTE PERIOD aaiiQgp____________ S31.080 A. Primary Site A.J. Lanza Labs 2. D. DECREE I. SOCIAL SECURITY So. Ph.D. 521-52-6704 S. NEW TORE STATE 2. ?. TELEPHONE NUMBER SENATORIAL DISTRICT HUMBER 26 c. HEW TORE STATE AC (914 >351-4229 ASSEMBLY DISTRICT NUMBER 65 7. RfflEAROf INVOLVING ROMAN SUBJECTS A* X So B.__ Tee-Approved Data C. Tes-Pendlas Review TO B1 COMPLETED ST RESPONSIBLE AiaoaiSTEAlIVE AOTHORITY 8. APPLICANT ORGANIZATIONS (S) LO. NAME, TULZ, ADDRESS, AND TELEPHONE NO. Institute of Environmental Medicine OP amCIAl XH S05IHESS 0T7TCE WHO NYU Medical Center SinS ALSO BE NOTimD 17 AN AWARD IS MADE. 550 1st Ave., New York NY 10016 Jobe 1. Bellow, Vice President for Finance IRS # 135562309N TELEPHONE NUMBER 212 679-3200 ext. 3611 9. NAME, TITLE,-AND TELEPHONE BO. OP LI. IDENTITY ORGANIZATIONAL COMPONENT TO OITTCIAL (S) SIGNING f0 APPLICANT nBciwTvarymi T.A. Htsfcrald, Director RECEIVE OtZDIT TOR Z3SHTUTI0NAL GRANT css rnnscnoNS) gfftssiStiLpmUttih**1******** *1"TECzPSOiaFsriMLTi) 212 679-3200 2235 01 New York University Medlcel Center 12. CERTIFICATION AB ACCEPTANCE. WE, IRE UNDERSIGNED, CERTIFY THAT THE STATEMENTS HEREIN ARE 98 AID COMPLETE TC ISR BEST OP COE SSOWLZDG3 AND ACCZPT, AS TO ARY GRANT AWARDED, TB1 OBLIGATION 1M COMPLY WITH ITS 3EALTB RESEARCH COUNCIL TEEMS AND CONDITIONS O EZ7ECT AT IS TIME OP THE AWARD. SIGNATURES A. SIGNATURE OP PERSON NAMED SI HEM 2.A. DATE (SIGNATURES REQUIRED ON SACS C. C tO'tf'lt COPT- USE OARZ IRE. "PER." SIGNATURES SOT ACCEPTABLE.) B. SlQU^RS_g|^E3SCN NAMED SI HIM 9. DATE "AX' ^ ------ (NOTE: Principal investigator sust also pnfsss sad slga professional assurance at the end of research plan) "MONS 207520 Saction I RESEARCH OBJECTIVES Priority Area * __ 7 Ltst Nm_______ Lea Social Security f 521-52-6704 NAME AND ADDRESS 0f APPLICANT ORGANIZATION Now York University Medical Center, Inst, of Environmental Medicine 550 First Avenue, New York, New York 10016 NAME, SOCIAL SECURITY NUMBER, OTTICIAL TITLE, AND DEPARTMENT OP ALL PROFESSIONAL PERSONNEL ENGAGED ON PROJECT, BEGINNING WITH PRINCIPAL INVESTIGATOR Chun C. Lee, Ph.D., 521-52-6704, Research Scientist sad Adjunct Assistant Professor, Laboratory for Environmental Studios, Instituta of Environmental Medicina, NYU Medical Canter {914}351-5419 Thee. J. Kneip, Ph.D., 031-36-5155, Deputy Director, Laboratory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center Joseph O'Connor, Ph.D., 011-34-2794, Assistant Director, Labora tory for Environaiental Studies, Institute of Environaiental Medicine, NYU Medical Center TITLE GT PROJECT! THE ENVIRONMENTAL HEALTH IMPLICATIONS OF AEROBIC AND ANAEROBIC DECOMPOSITION OF POLYCHLORINATED BIPHENYLS (PCBs) IN THE HUDSON ESTUABY It is known that PCBs raeovered from anvironmental samples (e.g., water, sediments and organisms) are differant from comnercially producad products by having an incraased abundanca of isomars of high'chlorine content; isomers of low chlorina contant are readily metabolized by organisms at lower trophic levels, or are otherwise degraded in the environment. It is also known that PCS isomers containing 504 or more chlorina ara lass active, biologi cally, than the lower chlorinated isomers, hence those isomers having high chlorine content may be less toxic. Consequently, the health risk from environmental accumulations of PCBs may changa with time because of differences in the degradation rata. This study is proposed to investigate the mode and ratas of PCB degradation (Aroclor 1221, 1016, 1242 and 1254) by aquatic and estuarine fungi and anaerobic bacteria, and to assay tha relative toxicity of the PCB mixture remaining aftar micro bial action against some key Hudson River organisms. MOWS 207521 0 iPROJZCT COMTROIi Priority Area # 7 Luc Haas ^ee___________________ Social Security # 521-52-6704 BBC # (Office use only) Type of Application: X Bee Renewal of SBC # Total Direct Coat* Requested: First Tear S $11.080 Total S N.A. _ Dace Submitted November 1. 1978 Principal Investigator (~^un C. Lee< Ph.D. Applicant Insnitution *ork Oniversity Medical Center Research Grant Manager: Institute of Environmental Medicine, NYU Med- Center Ira S. Goodman - Assistant Director, Office of Grants t. m.cu.unfl X22 3 ossa phone Title of Proposal: Tha Environmental, Health Implications of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) in the Hudson Estuary DO HOT WRITE TB LOT_____________ ___ ______________ (I) Proper Signatures (2) Paeant/Copyrlght Agreement (Hfr-23) (3) Protection of Bnen Subjects (IBC-l) (4) Protection of Labor atory Aniaals (Latter) Date Received (3) Principal Investigator Assurance (Statement and Signatures) (6) Progress Report (Renewal) (7) Invention Report (BBC-21) (3) Atmtiei Invention Statement (BBC-22) Date Reviewed HONS 207522 szctxow n PSIORITT ARH #_ Luc Nss*Lee F&mucszD ccttfliaiaiiaa pgLtr-m bcbget ret first u-mdsts period Social Security # 521-52-6704 AMDTOT aiOirrSTID a> PERSONSE. TXTLL OP POSITION I 0T TEH OR CT0SX <-tr lav FRINGE RAIS Z TOTAL 20.Srto 8/31/79 S #1% afters 9/1/79 r,,, Lee___ Principal Investiaator 20% 4.486 974 5,460 j,.M. O'Connor Technical Advisor 5% N.C. -0- T, 5% N.C. -0- tRA Secretarial Helo 20% 2.407 523 2,930 TBA_________ . ion*. 12.000 .2.606 14,606 (2) CCTSCLTAar COSTS. a) sob toc*i ta.a^i s ijju. *-22.3*S roJE?!g3T triable (2) Subcecal -.n. __________ (3) Subcecal s 500 StgP^ga^hemicaIs. reagents. media suoolies. labelled and unlabelled PCBs _ ? Routine glassware and solvents rbromatocranhie auonlie*. Miscellaneous supplies (4) 9 duntotal TSA.73L domestic nrr and L. tne-eel wlfh ml 1amia9 ^'"1 tjriT-V C3) Subcecal T* 1,000 1,000 750 250 3,000 400 inn 500 * 3,000 inn (6) Subcecal T" (7) 0T3S. EZP5DTSXS (DEIATO nwrhgad MSA of total! S 4.184 (7) Suatocal S 4,184^ (3) 2 DQICX COSTS..................................................................TOTAL 3 4,134 S31.0S0 ? 3 HONS 207523 1 SECTION U BUDGET ESTTVATTS FOE FUTUH2 TZA3S DI3ECT COSTS OSL7 (Qaie Cants)* Priority Are* #____ 7 Laic Sm Lee Social Security # 521-52-67ns Description First Tur (Sut Ax_ Datailed Budget on Fata 3 Second ?tar (1) Fersonaal Coats, Including fringe benefit* $22,996 (2) Consultant Costa (Include fees, travel, ate.) -0- (3) Equipment (4) Supplies (3) Travel <) Patient Coats (7) Oehar Opense* (overhead 15% of total) ___ Loa .3*000 ___ 1M -0 4 > 184 TOTAL DIBZCZ COSTS $ 31.080 $ ma iSUKS: Justify all coats for the fine year for which the need any aoe be obvious. For facum jun, justify equipment eoats, as Mil as any significant dungs in any other category. (Uaa continuation page if needed.) Fiva hundred dollars is requested for tha purchase of a small variable shaJcer assambly for the gtowth of fungal and bacteria cultures. This piece of equipment is not available at the lAStitUtia NOTE: Completion of these colrau does not obligate the NTS Health Saaearch Council to future funding. Ac application for renewal oust ba filed each vear. Faga 4 HONS 207524 szcnov ii PrioriC7 Arca } 7 Laic Hast Social Securicp ) 521-52-5704 OTHER RESEARC3 SUPPORT Lijt all research support (or ach Individual including reguesd nov being com id and, at wall ai any proposals being planned, regardleie of revclanca Co chls applicaeion. Include alio enrrenc or pending concracts, (ellovshlp award!, research career program averdi, training grand, rcgardlesi of che aource of aupporc. Include clcle of projecc, funding sourca, amount of support, and invaicigacor'1 clan cosmlcnenc. List grant member(1) for all current support. Support for Lee Currane: New York State DEC - Monitoring of PCBs in the Hudson River June 1, 1978 - May 31, 1979 $44,000. 20 % of time. New York University Medical Center Institutional - The Anaerobic Degradation of PCBs by Aquatic and Estuarine Bacteria. July 1978 - March 1979. $5,000, no salaries. pending: New York State ERDA - Power plant Simulator Studies December 1, 1978 - November 30, 1979 $90,000. 10% of time Planned: u. s. NOAA-Sea Grant - Comparative Toxicity of of Environmental PCBs vs. Comercial Preparations of PCBs in Hudson River Food Pish. Approximately $75,000. U.S. EPA - A Proposal to Devise Clean Up procedures for Labor atory generated wastes. Approximately $10,000. Page 3 MOMS 207525 n r biographical sketch Priority Are* /___ t Heme Lee Social Security 0 521-52-6704 (Give Che following infomacion for ell professional personnel beginning with the Principal Investigator. Use continuation pages and follow the same general format for each person.) NAME TITLE B13.THDATE Chun C. Lee, Fh.D. Research Scientist and (Mo..Day, ?r.) 08/31/39 ____________________________________ ________ Adjunct Assist. Prof.________ PLACE OF BIRTH (City, State, Country) Present Nationality (If non- Sex U.S. citizen, indicate kind ^ Male Hong Kong of visa and expiration data) Female EDUCATION (Begin with baccalaureate training and Include postdoctoral) INSTITUTION AND LOCATION________ DEGREE TEAR SCIENTIFIC CONFERRED________ FIELD_________ Colo. State U. Ft. Collins, Colo. B.S. 1962 zoology U. of Miami Coral Gables, Fla. PH.D. 1969 Marine Science HONORS Achievement Award in Biological Science, Colo. State U. 1962. NDEA Fellow 1962-1965, U. of Miami. MAJOR RESEARCH INTEREST ROLE IN PROPOSED PROJECT Environmental Pollution Project Director RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, list training end experience relevant to area of proJact. Liat all ralavant publications.) See Attached Curriculum Vitae Page 6A moms 207526 BIOGRAPHICAL SKETCH ?"iority Area #__ Laic Haae Lee 7 Social Security # 521-52-6704 (Giva tha following information for all profaiilonal paraonnal bagirming t^rh ~ tha Principal Investigator. Uia continuation pagai and follow tha aaaa ganaral format for inch parson.) NAME TITLE BiRTHDATE Theo. j. Kneip, Ph.D. Dep. Dir. Laboratory for _________________________________ Environmental Studies (Mo..Day, Tr.) 12/20/26 PLACE 0? BIRTH (City, State, Country) Present Nationality (If oon- Sax St. Paul, Minnesota, USA U.S. citizen, indicate kind J. Mala of visa and expiration date) Peaala EDUCATION (Basin with baccalaureate trainins and include postdoctoral) TEAR SCIENTIFIC INSTITUTION AMD LOCATION DEGREE CONFERRED FIELD University of Minnesota University of Minnesota University of Illinois B.S. M.S. Ph.D 1950 1952 1954 Chemistry Chemistry Chemistry HONORS Phi Lambda upsilon, 1950 - Sigma Xi, 1954 MAJOR RESEARCH INTEREST ROLE IN PROPOSED PROJECT Environmental Pollution Technical Advisor RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, Jist training and experience relevant to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6B HONS 207527 BIOGRAPHICAL SKETCH Priority Are* It____ ^ L*st Name Lee Social Security # 521-52-6704 (Give the following information for all profesalonal pereonnel beginning with the Principal Inveatigator. Dae continuation pages and follow the ease general format for each person.) NAME Joseph M. O'Connor, Ph.D TITLE Assist. Dir., Laboratory for Environmental Studies B1RTHDATE (Mo.,Day, Yr.) 11/19/44 PLACE OF BIRTH (City, State, Country) Boston, Mass., USA Present Nationality (If non- a*S' indicate kind of visa and expiration date) Sex Male Fenal* EDUCATION (Begin with baccalaureate training and Include postdoctoral) TEAR SCIENTIFIC INSTITUTION AMD LOCATIONDEGREE CONFERREDFIELD College of Boly Cross Worcester, Mass. B.S. 1966 Biology State U. of New York at Albany Ph.D. 1971 Ecology/Physiology HQNORS Sigma Xi, Beta Beta Beta Biological Society MAJOR RESEARCH INTEREST Environmental Pollution ROLE IN PROPOSED PROJECT Technical Advisor RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, list training end experience rAevant to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6 C HONS 207528 RESEARCH PLAN P-iority Ari *______ 7 *-*at Na&e Lee Socil Security 521-52-6704 BIBLIOGRAPHY: Provide a detailed bibliography that is relevant to tbs researon project. List toy research perforated by the principal investi gator U) named in the application. Use continuation sheets ________________ if necessary. Ahmed, M. and D.D. Focht. (1973). "Degradation of polychlorinated biphenyls by two species of Achromobacter," Can. J. Microbiol. 19: 47. " Alexander, M. (1965). "Biodegradation: Problems of molecular recalcitrance and microbial fallibility," Adv. Apol. Microbiol. 7: 35. Alexander, M. (1975). "Environmental and microbiological problems arising from recalcitrant molecules," Microbiol. Ecol. 2_: 17. Aulerich, R.J., R.K. Ringer, H.L. Seagran, .and W.G. Youatt. (1971). "Effects of feeding coho salmon and other Great Lakes fish on mink reproduction," Can. J. Zool. 49: 611. Aulerich, R.J., R.H. Ringer, and S. Zwamoto. (1973). Reproduc tive failure and mortality in mink fad on Great Lakes Fish," J. Reprod. Fert. Suppl. 19: 365. Bruckner, J.V., K.L. Khanna, and H.H. Cornish. (1973). "Biological responses of the rat to polychlorinated biphenyls," Toxicol. Appl. Pharmol. 24: 434. Bryant, M.P. (1972). "Cooaentary on the Hungate technique for culture of anaerobic bacteria," Amer. J. Clin. Nutrition 25: 1324. " Cahn, P., J. Foehrenbach, and W. Guggino. (1977). "PCB levels in certain organs of some feral fish from New York State," Zn: Physiological responses of marine biota to pollutants. r.J. Vernberg, A. Calabrese,Thurberg, aniPw.B. Vernberg (eds.), Academic Press, Inc., New York, N.Y., p. 51. Choi, P.5.K., H. Neck, and J.E. FI inn. (1974). "Distribution of polychlorinated biphenyls in an aerated biological oxidation : .water treatment system," Bull. Environ. Contamin. Toxicol. 11 12 Corcoran, V.F., and C.C. Lee. (1970). "The distribution of chlorinated hydrocarbon pesticides in the Mississippi River delta and the adjacent Gulf of Mexico," Joint Southeast and Southwest ACS Meeting, New Orleans, La., p. 6a. Duke, T.W., J.I. Lowe, and A.I. Wilson. (1970). "PCB (Aroclor 1254) in the water, sediment, and biota in Escambia Bay, Florida," Bull. Environ. Contamin. Toxicol. 5: 171. 7 Rage 7 HONS 207529 "'iicinuatlon Sl-JC Last Mum Social Security <) 521-52-471.1 Ecobichon, D.J. (1976). "Enzymatic and other biochemical res ponses to selected PCBs," Wat11 Conf. on Polychlorinated Biohenyls, Chicago, Illinois, p. 57. Fulk, R., D. Gruber, and R. Wullschleger. (1976). "Laboratory study of the release of pesticide and PCB materials to the water column during dredging and disposal operations," Final Rept. of u.S. Army Engineer Contract No. OACW39-7455142. Furukawa, K. and F. Matsumura. (1976). "Microbial metabolism of polychlorinated biphenyl components by Alkaligenes sp.," J. Aqri. Food Chen. 24: 251. Gibson, D.T. (1968). "Microbial degradation of aromatic com pounds," Science 161i 1093. Guenzi, W.D. and H.E. Beard. (1967). "Anaerobic biodegradation of DOT to DDD in soil," Science 156: 1116. Halvorson, H., M. Ishaque, J. Solomon, and O.W. Grussendorf. (1971). "A biodegradability test for insecticides," Can. J. Microbiol. 17* 585. Hague, R. and D. Schaedding. (1975). "A method of measuring the water solubility of hydrophobic chemicals," Bull. Environ. Contain, and Toxicol. 14* 13. Hile, J.P. (1977). "Polychlorinated biphenyls (PCBs): Dnavoid able contaminants in food and food packaging materials: reduction of temporary tolerances," Federal Register 42: 17487. Bullar, T., R.M. Pleasant, S. pagano, J. Spagnoli, and W. Stasiuk. (1976). "PCB data in Hudson River fish, sediments, water and wastewater," Report prepared for PCB Task Force, New York State Health Advisory Council. Humphrey, H.H.B., H.A. Price, and M.L. Budd. (1976). "Evaluation of changes of the level of polychlorinated biphenyls (PCBs) in human tissue," Final Rept. of FDA Contract No. 223-73-2209. Hungate, R.E. (1969). "A roll tube method for cultivation of strict anaerobes," In: Methods in Microbiology 38: 117. J.R. Norris and D.W. Ribbons Teels.), Academic Press, Inc., New York, N.Y. Hutzinger, O., S. Safe and V. 2itko. (1974). The Chemistry of PCBs, CRC Press, Cleveland, Ohio, p. 197. Pag* 8 MONS 207530 :ntinujcic:i SI tfC Laet __ Social Security '/ 521-52-6~04 Hydroscience Inc. (1978). "Estimation of PCS reduction by remedial action on the Hudson River ecosystem," Report to the Sew York Department of Environmental Conservation, pp. 107. Jensen, S. (1966). "Report of a new chemical hazard," New Scientist 32: 612. Kaiser, K. and P. Wong. (1974). "Bacterial degradation of polychlorinated biphenyls. Identification of some metabolic products from Aroclor 1242," Bull. Environ. Contamin. Toxicol. 11: 291. Kearney, P.C. and D.D. Kaufman. (1969). The Degradation of Herbicides, Marcel Dekker, Inc., New York, N. Y. Kimbrough, R.D. (1976). "Pathological findings associated with chronic experimental exposure to PCBs," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, Illinois, p. TJ7 Krull, I.S. (1977). "Recent advances in PCB analysis," In: Residue Reviews 66: 185. P.A. Gunther and J.D. Gunther (eds.), Spnnger^Verlag, New York, Inc. Kuratsune, M., T. Yoshimura, J. Matsuzaka, and A. Yamaguchi. (1972). "Epidemiologic study on Yusho, a poisoning caused by ingestion of rice oil contaminated with a commercial brand of polychlorinated biphenyls," Environ. Health Perspectives 1,: 119. Kuratsune, M., Y. Masuda, and J. Nagayaaa. (1976). "Some of the recent findings concerning Yusho," Nat11 Conf. on polychlorinated Biphenyls, Chicago, 111., p. 14. Lee, C.C. and R. Sansur. (Unpublished data on the growth of an aquatic fungus on Aroclor 1221, 1016, 1242 and 1254.) New York University Medical Center. Liu, D. (1976). "PCB degradation," Mar. pollut. Bull. 7: 83. MacRae, I.C., K. Raghu and E.M. Bautista. (1969). "Anaerobic degradation of the insecticide lindane by Clostridium sp.," Nature 221: 859. Matthews, H.B. and M. Anderson. (1975). "Effect of chlorination on the distribution and excretion of polychlorinated bi phenyls," Drug Metab- Dlspos. 2* 371. Matthews, H.B. and M. Anderson. (1976). "PCB chlorination versus PCB distribution and excretion," Nat'1 Conf. on Polychlorinated Biahenvls, Chicago, 111., p. 5^ Pg 9 MOMS 207531 utinuaeion SI "cc Use Menu Social Security <) 521-52. Munson, T.O., H.D. Palmar, and S.M. Forns. (1976). 'Transport of chlorinated hydrocarbons in the upper Chesapeake Bay," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 213. Nadeau, R.J. and R.A. Davis. (1976). "Polychlorinated Biphenyls in the Hudson River (Hudson Falls-Fort Edward), New York State," Bull. Environ. Contaain. Toxicol. 16a 436. Nebeker, A.V. and F.A. Pugles. (1974). "Effect of polychlori nated biphenyls on survival and reproduction of Daohnia, Gammarus, Tanytarsus," Trans. Amor. Fish. Soc. 7ti- New York State Dept, of Environmental conservation. (1976). "In the matter of alleged violations of Sections 17-0501, 17-0511 and 11-0503 of the Environmental Conservation Law of the State of New York by General Electric Company," Interim Opinion and Order File No. 2933. Nimmo, D.R., D.J. Hansen, J.A. Couch, N.R. Cooley, P.R. Parrish, and J.I. Lowe. (1975). "Toxicology of Aroclor 1254 and its physiological activity in several estuarine organisms," Arch. Environ. Contamin. Toxicol. 3^ 22. Nisbet, I.C.T. and A.F. Sarofim. (1972). "Rates and routes of transport of PCBs in the environment," Environ. Health Perspectives Is 21. Norback, D.H. and J.R. Allen. (1972). "Chlorinated aromatic hydro carbon induced modifications of the hepatic endoplasmic reticulum: concentration membrane arrays," Environ. Health Perspectives 137. O'Connor, J-M-, L. Peters, and R. Califano. (unpublished data on the uptake on Aroclor 1254 from Hudson River water and Hudson River forage by Morone saxatilis.) New York University Medical Center. Raghu, K. and I.C. MacRae. (1966). "Biodegradation of the gamma isomer of benzene hexachlori.de in submerged soils," Science 154; 263. Risebrough, R.w. (1969). "Chlorinated hydrocarbons in marine ecosystems," In: Chemical Fallout. M.W. Miller and G.G. Berg (eds.j, C.C. Thomas, Springfield, 111., p- 5. Risebrough, R.W. and B.W. de Lappe. (1972). "Accumulation of polychlorinated biphenyls in ecosystems," Environ. Health Perspectives 1: 39. Roach, J.A.G. and I.H. Pomerantz. (1974). "The finding of chlorinated dibenzofurans in Aroclor PCBs of recent Page 10 HONS 207532 Social Security ] 521-52-G;n-: As with the chlorinated pesticides the PC3s are relatively insoluble in water and are highly persistent in the environ ment (Risebrough and de Lapps, 1972). These compounds are not easily metabolized by enzyme systems presently found in nature (Choi et al-, 1974; Alexander, 1975) and tend to accumulate in fatty tissues and oils of animals (Risebrough and de Lapps, 1972). Because of their low water solubility, high specific gravity and high adsorbability, PCBs accumulate in anaerobic or reducing sediments of rivers, estuaries and the coastal zone. (Duke at al., 1970; Fulk et al., 1976; Munson et al., 1976). Although PCBs were first detected as environmental con taminants in 1966 (Jensen, 1966), their potential impact as a health hazard was not realized until 1969, after the discovery and description in Japan of Yusho, a human disease caused by consumption of PCS-contaminated rice oil (JCuratsune et al., 1972). Since then, PCBs have been implicated in environmental and occupational diseases ranging from ehloraene and liver damage to reproductive failure. They may also act as carcinogens or co-carcinogens (Norback and Allen, 1972; Kuratsone et al., 1972, 1976; Kimbrough, 1976; O.S. Department of Health, Education and Welfare, 1979). Laboratory studies with industrial preparations of PCBs (e.g., Aroclor 1242, 1254 and others), and with pure isomers of chlorinated biphenyls have shown PCBs to be highly toxic P9 13 HONS 207533 Social Sccyricy 521-52-*-^ to many aquatic and terrestrial organisms (Bruckner et al., 1973; Grant and Phillips, 1974; Stalling and Mayer, 1972; Nebeker and Pugles, 1974; Nimmo et al., 1975; Vos, 1972; Walker, 1976). Those organisms which are not immediately affected by PCBs may accumulate them to high concentrations, thereby increasing the exposure of other organisms through food chain transport (Risebrough, 1969; Cahn et al., 1977; O'Connor et al., unpublished data). It has been shown that the toxicity of a given PCB depends upon the degree of chlorination and its isomeric form. Chlorinated biphenyls that have a low percentage of chlorination tend to have a high acute toxicity compared to chlorinated biphenyls with a greater percentage of chlorina tion (Matthews and Anderson, 1975). The results from compara tive studies of chronic exposures of animals to specific isomers are confusing and inconclusive, but the exposure of test animals to subacute levels of individual PCB preparations (e.g., Axoclor 1242, 1248, 1254, and 1260) resulted in varying degrees of liver dysfunction, which was inversely related to the chlorine content of the Aroelor (U.S. HEW, 1978). Lower homologs are also usually more soluble in water than the higher ones (Haque and Schmedding, 1975), and are therefore more available to, and more rapidly absorbed and metabolized by, aquatic organisms. PCBs which can be metabolized and excreted may introduce toxic metabolites to the environment, while the forms of PCB that are not readily Page 14 MONS 207534 Co.iLi-u.icio.i 31 QC Social Sec-ric, ! manufacture," presented at 85th Annual Meeting Assoc. Official Analytical Chemists, Paper No. 53, Washington, D.C. October 14-17. Sayler, G.S., M. Shon and R.R. Colwell. (1977). "Growth of an estuarine Pseudomonas sp. on polychlorinated biphenyl," J. Microbial Ecol. 51 JTl. Saylar, G.S., R. Thomas and R.R. Colwell. (1978). "Polychlori nated biphenyl (PCB) degrading bacteria and PCB in estuarine and marine environments," Estuarine and Coastal Marine Science 6: 553. Stalling, 0. and J. Huckins. (1973). "Reverse phase thin layer chromatography of some Aroclors, Halowaxes, and pesticides," J.A.O.A.C. 56: 367. Stalling, D. and F.L. Mayer. (1972). "Toxicities of PCBs to fish and environmental residues," Environ. Health Per spectives 1: 159. United States Department of Health, Education and Welfare. (1977). "NI05B criteria for a recommended standard...occupational exposure to polychlorinated biphenyls (PCBs)," OtffiW (NIOSH) publ. Ho. 77-225. Vos, J.G. (1972). "Toxicology of PCBs for mammals and birds," Environ. Health Perspectives It 105. Vos, J.G., J.B. Koeman, H.L. Van dar Mass, M.L. Ten Hoever de Brauw, and R.B. de Vos. (1970). "Identification and toxi cological evaluation of chlorinated dibenzofuran and chlori nated naphthalene in two commercial polychlorinated bi phenyls," Food Cosmet. Toxicol. : 625. Walker, C.R. (1976). "The occurrence of PCBs in the national fish and wildlife monitoring program," Hat11 Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 161. Wiese, C.S. and D.A. Griffin. (1978). "The solubility of Aroclor 1254 in seawater," Bull. Environ. Contamin. and Toxicol. 19: 403. Wong, P. and K. Kaiser. (1975). "Bacterial degradation of polychlorinated biphenyls II. Rate studies," Bull. Environ. Contamin. and Toxicol. 13: 249. P? 11 MOHS 207535 tinuacion Si cc Social Security 7 521-S 2-c .i A. INTRODUCTION 1. Objective; This research will investigate the rates at which aerobic or anaerobic microorganisms degrade PCBs in rivers, estuaries, or landfills. The investigation is designed also to test the hypothesis that the toxicity of PCB residues is gradually lessened in situ by the fact that the lower chlorinated isomers are more rapidly degraded than the higher chlorinated isomers which are known to be less active biologically and less toxic as well. 2. Background; Polychlorinated biphenyls (PCBs) are among the most persistent contaminants man has introduced to the environment. These compounds are a versatile group of industrial chemicals that have been widely used for more than 40 years. Two-hundred-nine possible isomers of chlori nated biphenyls exist, and each industrial PCB preparation may contain ten or more isomers, as well as impurities, such as polychlorinated bibenzofurans (PCDFs), polychlorinated dibenzodioxans (PCDDs) and other unidentified products (Vos et al., 1970; Roach and Pomerantz, 1974; Hutzinger et al., 1974). These products have been released into the nation's fresh and coastal waters by deliberately discarding PCBs or PCB-laden products into landfills and dumps, or as effluents into rivers (Nisbet and Sarofim, 1972). A small amount is lost into the air from evaporation from plasticized products or from incomplete burning of substances containing PCBs. M0N5 207536 12 Social Security '/ 521-52-',"'?.! metabolized have an extremely long biological half-life (Matthews and Anderson, 1976). It has been shown also that isomers having two adjacent unsubstituted carbons in the biphenyl ring are more readily metabolized than ones without the two adjacent unsubstituted carbons (Ecobichon, 1976). The net effect of the above is that different PCBs have different toxieities and different rates of bioaccumulation and persistence. The evidence in general establishes that PCBs with five or more chlorine atoms are less biodegradable and are accumulated to higher levels in organisms and persist longer in the environment. PCBs from aquatic environ mental samples, such as fish, show on analysis a pattern of peaks approaching that of PCB mixtures with more than 50t chlorination, while the major input of PCBs into the environ ment was PCB mixtures containing 42% or less chlorine (Matthews and Anderson, 1976). Highly chlorinated Aroclors, those containing 50% or more chlorine, comprised approximately 30% of sales prior to 1971; this figure was expected to decrease to 22% after 1971 (Nisbet and Sarofim, 1972). This lends support to the laboratory results described above, that the rate of metabolic attack on PCBs decreases with increasing chlorination. It is therefore possible that PCB residues found in natural waters and sediments and in aquatic organisms which concentrate PCBs via long food chains may not have originated entirely from one specific industrial preparation such as Aroclor 1242, 1016 or 1254. Instead, they may Pag* 15 HONS 207537 ' ''t -IL.L > Social S':,--r'. ' 32 1 - 3 2 -'" represent only the higher homologs of several pea preparations, tne lower homologs of which were metabolized or otherwise degraded along the food chain. The consumption of fish contaminated with PCBs from polluted lakes and streams appears to be the major source of PC3 exposure for man and animals. However, there is no record of clinical damage to humans from such consumption (Humphrey et al., 1976; Hile, 19771. even though the amount of PCBs ingested was high compared to people who ate no fish. This being the case, it becomes unclear whether this lack of adverse health effects from the consumption of fish contaminated with PCBs from the environment is due to the low level of exposure or because the PCBs in the fish are primarily isomers that have a low-grade toxicity; if so, the more active, more toxic isomers may have been degraded in the environment to less toxic forms. The persistence of some chlorinated pesticides and hydrocarbons and the apparent stability of some of these compounds in the environment fostered the idea of molecular recalcitrance (Alexander, 1965, 1975). As a result, the biodegradation of PCBs has received little attention and few data exist on the rate at which PCBs are being degraded in nature. However, it has been concluded by many investigators from laboratory studies that PCB isomers containing four or fewer chlorine atoms are degraded rapidly in the environment by microbial activity. Sayler et al. (1978) showed that PCB HONS 207538 16 CcuLlnu.u.Ia.1 L1 Social S'jcarit ^21-52-^- resistant, or degrading, bacteria were ubiquitously distributed and indicated the potential for PCB degradation by natural microbial flora in estuarine and marine environments. Available reports on PCB metabolism by microorganisms have dealt exclusively with aerobic bacterial systems (Ahmed and Focht, 1973, Kaiser and Wong, 1974; Wong and Kaiser, 1975; Furukawa and Matsumura, 1976; Liu, 1976, Sayler et al., 1977, 1978). Published works which indicate that chlorinated pesticides and other aromatic compounds are degraded under anaerobic conditions (Raghu and MacRae, 1966; Guenzi and Beard, 1967; MacRae et al., 1969; Focht and Ahmed, 1970) suggest that the lower PCB isomers in sediments may be degraded primarily by anaerobic bacteria. Work begun in our laboratory with an aquatic fungus (Lee and Sansur, unpublished data), isolated from Hudson River water and grown in Aroclor 1254, together with the works of Sayler et al. (1978) and Kearney and Kaufman (1969) indicate that fungi may also be actively involved in the PCB degradation process. 3. Rationale: PCB contamination in the Hudson River is probably the most extensive in the nation. There are an estimated 450,000 to 600,000 pounds of PCBs in the bottom sediments of the upper Hudson River between Fort Edward and Troy, New York. Although no reliable estimates of PCB content in the bottom deposits of the lower Hudson from Troy to New York city are available, PCB content is expected to HONS 207539 17 (.nnLiniiai ton i :C Social ioruric,1 CJ &T !- * -J -c - ^ - be substantial. The major portion is adsorbed to particles deposited m anaerobic or reducing sediments near the point of PCB discharge. Even without further discharge, these PC3s will remain for some years and will continue to contami nate river biota unless the PCBs are degraded or are removed by water currents, dredging or by bioaccumulation in aquatic organisms. Thus far, degradation studies have centered on aerobic bacterial activity, whose results have little appli cation to the problem at hand, because most of the PCBs are sequestered in anaerobic or reducing sediments. Until now, the role of fungi in PCB degradation has not been considered at all. This study is proposed to complement the existing work on PCB degradation to achieve a more complete understand ing about the fate of these PCB deposits. As man is the ultimate consumer after eating contaminated fish, and in light of the uncertainties described above regarding the differential toxicities of different PCB preparations, it is our intent, also, to examine the toxicity of the PCB mixture remaining after microbial action on key Hudson River organisms relative to the documented toxicities of non-degraded PCB mixtures. This information will make it possible to predict the future public health and ecological significance of the PCB deposits. HONS 207540 TQ Cr.iLi'.iMLlOtl L "t Liul - ,. Soct.il S'jt-uri: / 521-52-"' B. SPECIFIC AIMS 1. To isolate and cultivate from Hudson River sediments anaerobic bacteria and fungi which are able to grow m the presence of pCSs (Aroclor 1242, 1016 and 1254) and to investigate their ability to degrade then. Initially, this will be done with mixed cultures; studies will be done with pure cultures as they become available. Aerobic bacteria will not be included in this work, as these organisms are being studied by other investigators. 2. To determine the rate of PCB degradation by anaerobic bacteria and fungi thus isolated and to characterize the resulting metabolites and residues. 3. To examine the relative toxicity of the PCB mixture remaining after microbial action against non-degraded PCB mixtures on some key Hudson River organisms such as flaaanarus, Neomysis and striped bass larvae. C. METHODS 1. Procedures for Specific Aim 1; Sediments for the isolation of anaerobic bacteria and fungi will be collected from at least two sites in the Hudson River known to be contaminated with PCBs, one in fresh water and the other in the saline portion of the river, with a sediment grab lowered from a boat. Immediately upon collection, the sediment will be transferred to duplicate sets of glass bottles which will be almost filled with filtered river water and sealed with serum caps. One set will be gassed with compressed nitrocen HONS 207541 ia JiiLInii.iLlcii , L Social S>:f yrit/ 1 521-32---H to maintain reducing conaitions necessary for anaerobes; the other will be maintained aerobic for fungal isolates. Both sets of samples will be returned to the laboratory for subsequent isolation of PCB degraders by enrichment culture. Enrichment cultures for anaerobic bacteria will be set up by adding 1-2 g of sediments to a 250 mi flask containing 100 mi of an organic medium*, glucose, 0.5 g; peptone, 0.5 g; KH^PO^j, 0.5 gj distilled water or water of appropriate salinity, 1 i (pB 7.0) . Techniques for anaerobic cultures as described by Bryant (1972) will be strictly followed. Cultures will be incubated for 3 days at 26C; intermittently, culture flasks will be shaken by hand. Isolation will be carried out in a second flask which contains 100 mi of a mineral salt solution: (NHj^SO^, 1 g# KHjPO^, 0.2 g; KjHOP^, 1.6 g; MgSOj^l^O, 0.2 g; NaCl, 0.1 g; FeS0^*7H20, 0.01 g; CaCl2-2H20, 0.02 g (pH 7.5) containing 0.05% of the appropriate PCB. Following standard anaerobic techniques 1 ml of the enrichment culture will be transferred to this second medium and incubated as before until substantial growth is observed. Such cultures will be maintained in duplicate or triplicate. One series will be incubated in the light and one in the dark to enable the growth of phototrophic bacteria as well as strict eheaotrophs and heterotrophs. Enrichment cultures for fungi will be started in Sabouraud dextrose agar (Difco) and incubated under aerobic conditions. After initial growth, 1 mi of the enrichment culture will be MOMS 207542 20 Sac i.i l . transferred to the PCB-mineral salts medium (described above) but amended with 1 ml of a trace elements solution per liter. The trace elements solution contains o.i g H3303, 0.05 g each of CuS04*5 HjO, MnS04*H20, 2nS04*7 HjO, Na^MoO^, and CoCl^'S H^O per liter. After incubation for predetermined time intervals (4 days to 1 month), aliquots of the incubated samples will be transferred to separatory funnels and extracted with hexane. Gas chromatograms will be run with 1-5 til aliquots of the hexane extracts. Initially, only the disappearance of certain PCB isomers from the mixed culture systems will be measured. If the PCBs disappear or decrease in quantity, degradation products will be looked for, perhaps using thinlayer chromatographic techniques and with 14 C-labelled PCBs. Particular emphasis will be placed upon the water-soluble products. These will be isolated, after hexane extraction, by column chromatography on ion-exchange columns and resolved by thin-layer chromatography. Mixed cultures will be purified by plating onto mineral salts agar plates containing O.OSt PCBs. 2. Procedures for Specific Aim 2; Once the respective microbial systems capable of growth on PCBs are obtained, the kinetics of PCB degradhtion will be followed by analyzing the PCBs in the growth medium at different time intervals. The cells will be harvested by centrifugation and washed twice with 0.5 m phosphate buffer (pH 7.0). The washed cells will be MONS 207543 21 ioc:^.: S'i'-.zLz, ' 521-32-'- injected into culture tunes containing the mineral salts medium and PCB. The procedures employed will be similar to those described by Halvorson et al. (1971) who used resting suspensions of bacteria harvested from a sewage lagoon to examine the degradation of mixtures of pesticides. Comparisons will be made of gss chromatograms of PCBs before and after incubation with test organisms over the predetermined time interval. Metabolites and residual PCBs will be characterized by the use of column and thin-layer chromatographic techniques {Stalling and Buclcins, 1973; Furukawa and Matsumura, 1976) . 3. Procedures for Specific Aim 3s PCB residues and residual PCB isomers from microbial activity on the original Aroclor will be recovered from thin-layer chromatograms by scraping the respective spots off the plates and dissolving m acetone. Their toxic effects, if any, will be assessed on the physiology and population dynamics of some key Hudson River organisms (Gammarus, Neomysis and striped bass and other fish larvae) relative to the non-altered Aroclor. These organisms represent important fish food items and at times comprise the majority of zooplanktonic or benthic species in various portions of the Hudson River, and are routinely cultured in our laboratory. Cultures of each species will be established and exposed to varying levels of PCBs, PCB residues and metabolites in static systems. Biological effects of PCB exposure will be estimated from several critical parameters: HONS 207544 22 "euLiau.iiJc.i '*t r. i,,; Social ^21 -- 5 2 -- t a. rate of individual growth b. rate of respiration c * rate of production of young d. limits of tolerance to selected stresses: e.g., chlorine exposure, osmotic stress, thermal stress All of the above parameters will be estimated for cultures receiving PCB stress, as well as those cultured m the absence of PCBs. The data gathered here will be analyzed following basic toxicological approaches to threshold sensi tivity of organisms to toxicants and will be subjected to standard parametric and nonparametric statistical tests. 4. The research tasks will be performed in the following sequence, assuming a beginning data of January 1, 1979: January to March: Sample collection and culture isolation March to July: Degradation experimentation and analysis July to October: Toxicity experimentation Novesiber and Decsmber: Report preparation D. SIGNIFICANCE In light of our present knowledge about the toxicological properties of certain PCB isomers on living organisms and the knowledge that there might exist different rates of degradation for each of the 209 PCB isomers, it is expected that the health risks from PCB accumulations may change with time. This study should provide data for the assessment of long' term health risks from PCBs and their residues in the aquatic environment. MQNS 207545 23 bocl.il S 521 E. FACILITIES AVAILABLE The proposed research will be carried out using the facilities of the analytical chemistry and aquatic ecology sections of the Laboratory for Environmental Studies. The proposed research will be carried out in the culture and analytical facilities at the A.J. Lanza Laboratory, Tuxedo, New York, and at the Hudson River field station maintained by the Medical Center at Buchanan, N.Y. NYU boats equipped for collection of samples and certain field analytical procedures are berthed at Stony Point, N.Y. and operate throughout the open-water months. Dock facilities are available at the Buchanan wet-lab, but only on a temporary basis. Equipment available for this project includes apparatus for: Extraction/concentration of organics Freeze-dry techniques Scintillation spectrometry (low level - beta) Gel electrophoresis Thin-layer chromatography Gas-liquid chromatography High-pressure liquid chromatography Respiration studies Water quality analysis Autoradiography HONS 207546 >& Social Ser.urii/ ! 521-52 Culture facilities and equipment for aerobic and anaerobic microorganisms Culture of aquatic organisms Maintenance of aquatic organisms Computer data analysis F. PRINCIPAL INVESTIGATOR ASSURANCE "The undersigned agrees to accept responsibility for the scientific and technical conduct of the research project and for provision of required progress reports if a grant is awarded as a result of this application." /O' J7- 7? Date Pag* 25 HONS 207547 SEW TORS STATE SALIH StlSEiaiT IDCSCIL FEUIECTIOH OF HQNtf SUBJECTS Assuranc e/Cartificatlon/Deelaration (Pursuant co Federal and State Law) *7 Lee 521-52-670 Q Crant (""I Fallow STAlEJfETr OF POLICE: Safeguarding the rights and vslfare of subjects at risk la ac*JvJ-Jt* supported under crane a and contract from STSHEC ia primarily the responsibly-, 9* *he*ir3--- tion which receives or la accountable to MTSH&C for eha funds avardad for the'suooorr o' --l" aceiviry. In ordar eo provide for ehe adequate discharj* of this Institution*! reapjcsloil.t ie U eha policy of 5TS3HC ehae ao activity involving hiinaa suojeet* eo ba suppor*ad o* ttiz?. Crane* or concrace* snail be undertaken unless eha Institutional Bsvlev Board Sm rrriuaa an approved sue! activity, and the Institution hu submitted to 5TS33LC * certification of such review and approval, ia accordance wish ehe requirements of Chapter 450, NTS Uvi of 1575. L. TULL OF PROPOSAL OH ACITVnT; The Environmental Health Implications of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) -- '- Vi'iaT. - - - -- __ __________ __ 2. Principal Investigator/Aetivlcy Diractor/Fellow Chun C. Lee, Ph.D. 3. Declaration that Subjects Either Would or Would Sot Be Involved Cyj 1, He individuals vbo night be cooaldared human subjects, 1acladlac those f: when organs, tissues, fluids, or other aetarlals would be dgnved, or who could bo Identified by personal data, would be Involved In the proposed activity. (If eo tnaaa subsets would bo iavolvad. chock this bos and orocaod to Team 5. Proposals determined by the Agnecy to involve hitman subjects will ba returned.) B. Sbaan subj acts would, be Iavolvad In the proposed activity as cither: Q None of the following, or Including: ("1 Minors, ft Fernsss. i~1 Abortuses, i~1 Pregnant Woman, Q Prlsoaera, Q Mantally Eatarded, Q Mentally Disabled. Cndar Section 7, Cooperating Institutions, on reverse of this form, give name of institution and ossa and address of offlclal(s) authorising access to any subjects in facilities not under direct control of the applicant or offering institution. 4. Declaration of laanxsnca Stacua/Cartlflcatlou of Bavlew QJ This institution baa ant previously filed an assurance and assurance isplenanting precedurea for the protection of *-- subjects with tha Connell that applies co application or activity. Assurance Is hereby given that this institution will comply with requirements ef Chap car 450, 313 Laws of L97S, that it has established an institutional review board far the protection of human subjects and, whan requested, will submit documentation and certification of such reviews and procedures as may be required for Implementation of this assurance for the proposed project r-- or activity. 1 1 3. institution has am approved general assurance or an active special asauranea for this ongoing activity, on file with the Council. The aignera certify chat all activities in tfaf application proposing to involve subjects have been revtavec and approved by this institution's institutional review board in a convened seating on the data of__________________________ THE aSTlTUTTOSAL RF7TJW wiaan aAS DETSUffilED.AHD THE BISTITDHOHAl OFFICIAL SICNT'G 7`CCNCT3S THAI: Either Q human subjects will not be eg risk,or Q bunan subjects will ha it n 5. Nine and Acdrasa of Institution 6. Title of lascltutisn Official Signature ot Institutional Official Data ohcr.a: HONS 207548 Not App- JM* 1 - :. Official Titla(s) Business Address Facility White Invancion Hade Naas of Unit or Building Addrtaa DESCRIBE INVENTION IN DETAIL FOLLOWING OUTLINE BELOW. ATTACH MATS, SKETCHES, PRINTS, ?-C`CS TO DESCRIPTION. A. General Purpose - Stact in general tiraa che purpoaa and object of the invention B. Background - Deacribe prior are (Identify by pacenc nuaber or journal citation) C. Daacrlpelon and oparaclon 1. Deacribe completely the cone truecion of invention via lag ref trance characters co identify coaponenca in attached llluacrationa. 2. Deacribe one (1) complete operational cycle. 3. If Invention relataa to a eyncheele or Identification of a new composition of aetter, deacribe the product in structural fora, if possible, and the process of aalrlng It. Include all available information regarding physical characearlaclcs and all teat daea evidenclna utility. DESCRIBE COfMERCIAL POSSIBILITIES (INCLUDE QUANTITT AND PRICE RANGE I? AVAILABLE) STATE REASONS FOR APPLYING FOR PATENT STATE PLANS TO PUBLISH NAME OF PUBLICATION, ATTACK OUTLINE OF ARTICLE. IF ALREADY PUBLISHED, INCLUDE JOURNAL CITATION OR ATTACH REPRINT. DESCRIBE AND GIVE DATI(S) OF AMT SALE OR PUBLIC USE OF INVENTION (SPECIFY IF USE WAS OPERATIONAL OR FOR TESTING PURPOSES) CONTRIBUTIONS TO THE INVENTION BY: GRANTEE MYSHR COUNCIL OTHER (Identifyi PERSONNEL (No. of Personnel) _ MATE!LAIN (Liat Type and Value) FINANCIAL CONTRIBUTION _____________________________________________________ EXECUTION OF DISCLOSURE I, naaa of lnvantar . state the above inforaation with ragard to the invention o: title of invention the invention. a la a true and corract disclosure of data pertinent (1) ________________________ Technical Witness Signed (2) ________ Data HONS 207549 7. Cooperating Institutiona - Additional Reporting Requirement Cat Following Report Foraat for Each Institution Other Than Grantee or Csntraciir; Institution vieh Responsibility for rTiman Sub;acta Participating in this Activity: (Attach additional raport sheets as necessary) DfSTTTTTIONAL AUTHORIZATION FOR ACCESS TO SUBJECTS Subjects: Status (Wards, Residents, Employees, Patients, Etc.) Nuaber Age Range Title Nana and Address of Cooperating Institution Wtene Official Signature HONS 207550 PATS? iUD CCFTSISar ASSSSiETT TIi* andaraijn*'! applicant, la conaldaraeicn of * (mt/catas: luariad by cb* S*v Torx Seat* Saaleh Jaaaarnh Council, do** hereby agra* u fallow*: (*) To hasp eh* Conell laforrjad of *ay potentially ?*cttabla dv-.:* or t*chlaqu.* eencalnd or dmloptd by eh* uad*nlge*d, la vbol* or la port, Individually or with othor*. Cb) That * paeeatabl* drric* or eachlaqu* ccnealnd or dmlopad la eh* eaurs* or worfciag on pro(r*a* or prcjwes funded by eh* Cornell shall b* eh* property of sb* Coisell. Co) That tsyalElm, or ay ocher rwuaarxtian received by eh* applicant *111 b praapely nrteced es Eh* Council and ndiacrlbuced to eh* applicant aad eh* lonacor (s) p*r*uaac co Council policy. (d) Upon laacmceles of eh* Council, eo apply for a paesae os any ouch paeaaeabla drrlc* or ctcbalqu* and assign to eh* Council or lea aorta** all wy right, del* and ineaxvae la such paeaaeabla device aad ax*coca, ackaovladg* aad deliver eo sha Council any laserouants o*ca*aary or ragrtnd by eh* Council co affacenaea auch application, aaaignaane of ehla agrswaac. () To coafora with and adhara co eh* pactac and copyright policy of eha Cornell, * copy of which baa b**n ncaivad by eh* undersigned, a* eh* law say fro ela* eo ela* b* <a*adad. (f) To glv* approprlac* cradle co eh* (Totacli la any public*eloa or oeh*r fora of dlacloaur* or at eh* r*qu**t of eh* Council eo provide aa approprlac* dlaclaisar eo eh* efface that eh* puolicaeloa or dlacloaur* da** aoe r*pr***ae eh* official poaieloa of eh* Cornell. (g) k description of any lsrutlaB, uhaehar aad* or coscalved by aploy** or *|*sea of eh* ndsral^ed prior eo funding by eh* Council, *heh*r pacantad or aoe, la appandad banco aad auch laveaeiona shall ba excluded from ehla agr3*ae, I (w) rapnane chat eha abanc of any IsnaEian la eha aceachad schsdula shall lcdlcac* ehac I (va) own co auch lanation ae eh* Ela* of signing ciis ipuant. (Sijna run) /o (Seta) HONS 207551 APPENDIX: NYU Organism Culture The New York University Medical Center presently maintains in culture several aquatic species, including protozoa, algae, zooplankton, benthic biota, fish, and fish larvae. The species of algae in culture include: Chlorella , Nanr.ochloris , Chlaiavdcmonas, Melosira , Svnedra. Anacvstis . and others. Protozoan species include Stronbidiun. Euolotes, Ursr.vc'-ii, and Paramecium- Many different types of micro zooplankton have been maintained at the Medical Center. Included among them are: Mesocycloos edax, Eurvtemora affinis, Acartia c-iausi, Oaohnia magna, and Cyclozs bicuspidatus. Ostracods are presently being cultured in the laboratory for a study of breeding behavior. Benthic biota maintained at the Laboratory include Carcinus maenas. Rlthrooanopeus harrisi, and various species of gastropods. The crabs have been used in a series of PC3 uptake studies, as well as in studies of respiration as affected by hydrogen sulfide and low oxygen tension. Several macro zooplankters are maintained at the Laboratory at ell times These include Gammarus daiberi, B. tlgrinus, G. fasciatus. Neomvsis americana, and the larvae of Chaoborus punctipennis. The three species of Gaimiiarus have been maintained for more than four year* in aquarium culture, and in incubators at a variety of different temperatures. Morone saxatllis. Cvorinus carpio, Fundulus heteroclitus. and Microcadus tomcod are examples of fishes and fish larvae which have been raised and maintained in our laboratory facility. Striped bass and tomcod have been spawned and raised in NYU facilities each year since 1975. These laboratory-raised organisms have been used successfully in studies of temperature tolerance, chlorine toxicity, behaviour, and growth. The following references provide examples of studies conducted by t.*e HONS 207552 NYU staff using organisms produced at, or maintained in, nyu c facilities: 1. THESIS EicJ'.orn, K.L., 1977. The response of striped bass {Mcrcne saxatilis) to heated and chlorinated power plant effluent. M.S. Thesis, Biolgoy Dept. New York University. Ginn, T.C., 1977, An ecological investigation of Hudson River macrozooplankton in the vicinity of a nuclear power plant. Ph. D. Diss., Biology Dept., New York University. Jinks, S.M., 1975. An investigation of the factors influencing radiocesiura concentrations of fish inhabiting natural aquatic systems. Ph. D. Diss. Biology Dept., New York University. Paschoa, A.S., 1975. The natural radiation dose from radium to Gammarus in the Hudson River. Ph.D. Diss. Sch. Eng. and Science, New York University. Poje, G.V., 1977. Growth and reproduction of Gamnarus tiqrinus Sexton. 1939. M.S, Thesis., Biology Dept. New York University. 2. PUBLICATIONS Beck, A.P.., G. Poje, and W. Waller. ,1975. A laboratory study on the effects of exposure of some entrainable Hudson River biota to hydrostatic pressure regimes calculated for the proposed Cornwall Pumped Storage Plant, pp. 157-204 In: S. Saila, ed.. Fisheries and Energy Production. D.C. Heath, Lexington, Mass. Becker, A.J., 1977. The respiratory responses of the green crab Carcinus maenas to reduced partial pressures of oxygen. Limnol. Oceanogr. in press. Ginn, T.C. and J.M. O'Connor, 1978 {in press). The responses of Gammarus tiorinus to chlorinated power plant effluents. Estuarine Coastal Mar. Sci. Ginn, T.C., W. Waller, and G.J. Lauei., 1974. The effects of power plant condenser cooling water entrainment on the amphipod Gammarus spp. Water Res. 8:937-945. Ginn, T.C.- W. Waller, and G.J. Lauer., 1976. Survival and recr duction of Gamnarus spp. (Amphipoda) following short-term exposure to elevated temperatures. Ches. Sci. 17:8-16 HONS 207553 O'Connor, J.M. and S. Schaffer. 1977, Survival of striped bass ichthyoplenkton in nets. Ches. Sei., 18:312-315. Poja, G.V., T.C. Ginn and J. M. O'Connor. 1978. Responses of ichthyoplenkton to s trass as simulating passage through a sowar plant condansar tuba. In: Thorp, J. and J.W. Gibbons (eds.l. Energy and environmental strass in aquatic systems. Symposium of tha Savannah Rivar Ecology Laboratory, 1977. Rachlin, J., A.P. Back and J.M. O'Connor. 1978. Karyotypic analysis of Hudson Rivar stripad bass (Morons saxatilis). Copaia, I97a: in prass. Zubarik, L.S. and J.M. O'Connor. 1978. A radioisotopic study of mercury uptaka by Hudson Rivar biota. In: Thorp, J. and'j.w. Gibbons (ads.). Enargy and anvironmantal strass in aquatic systems. Symposium of tha Savannah Rivar Ecology Laboratory, 1977. ` 3. PUBLICATIONS IN REVIEW Ginn, T.C. and J.M. O'Connor. Tha distribution of Gammar-us daiban in tha Hudson Rivar astuary with a nota on a ranga extension for tha spacias. Crustacaana... O'Connor, J.M., K.L. Eichorn and T.C. Ginn. Behavioral response of larval and juvenile stripad bass to heated and chlorinated power plant affluents. Estuaries... O'Connor, J.M., G. Poje and S. Rigdon. Respiration of larval forms of tha striped bass (Morone saxatilis) following short-term ex posure to elevated temperatures"! Exp. Mar. Biol. Eeol... HONS 207554 A. PRESENTATIONS Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Rtipons* of the striped baas Wo rone saxatilia and the amphipod Gaanarus to chlorinated power plant effluent. Northeast Fish and Wild life Conference. April 26-29, Hersey, Pennaylvania. O'Connor, J.M., C.C. Coutant and J.P. Lawler. 1977. Environmental impact assessment aa ecological reaeareh. Symposium on Energy and Environmental Streaa in Aquatic Syatams. Augusta, Georgia, November, 1977. Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. oae of a condenaer tube simulator for evaluating power plant entreinawrt effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. . 1977. An operational system nmiatmg the physical and chemical environment of the con denser tubes of a power plant. Symposium on Energy and Wnvironmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tlgrinus; an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society, May, 1976. Zubarik, L.S. and J.M. O'Connor. 1977. A radioisotopic study of mareury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. HONS 207555 CURRICULUM VITAg January X978 Chun Chi Laa (Formerly Known as Xing Mon T$ua) Data and Place of Birth: Marital Statust Residence: Profassional Addrasst Titlat Education: Positions Kaldt Coursa Offaring: (Department of Environmental Medicine and Dapartaant of Biology) August 31, 1939 - Bong Kong Married, Two childran R.D. *2, Faltar Hill Rd. Monroe, Hew York 10950 Nav York Univarsity Medical Center Instituta of Environaantal Medicine 550 First Avenue Maw York, Maw York 10016 Telephone: (212) 679-3200, Ext. 5276 Research Scientist and Adjunct Assistant Professor Laboratory for Environmental Studies 1962 - B.S. in Zoology Colorado State Univarsity 1969 - Doctor of Philosophy in Marina Science Univarsity of Miami (Florida) Mew York Univarsity Madical Center Dapartaant of Environmental Medicine 1975 - Present Aquatic and Estuarine Microbiology Univarsity of Miami (Florida) Rosenstie1 School of Marina and AtsK>spharic Science, 1969-1975 Research Scientist, Functional Biology 1969 - 1973 Research Assistant Professor Biology and Living Resources 1973 - 1975 l HDNS 207556 Positions Held: Professions! Societies: Research Interests: Awards and Grants: University of Miami (Florida) Institute of Marine Science 1962 - 1969 NDEA Fellow, Marine Biology 1962 - 1965 Graduate Assistant 1965 - 1967 Senior Field Biologist McMurdo Sound, Antarctica 1967 - 1968 Senior Field Biologist First International Weddell Sea Oceanographic Expedition 1968 - 1969 Colorado State University Department of Botany and Plant Pathology 1958 - 1962 Research Assistant, 1958 - 1962 American Association for the Advancement of Science American Society of Limnology and Oceanography Microbiology Algal and Protozoan Physiology and Ecology Biochemistry of Organic Matter in Aquatic Sediments Achievement Award in Biological Science Colorado State University, 1962 National Defense and Educational Act Predoctoral Fellowship, 1962 - 1965 National Science Foundation Grant University of Miami, $44,000 1973 - 1975 National Science Foundation Grant New York University Medical Center, $3,900 1976 HONS 207557 PUBLICATIONS C. C. LEE 1. Meyers, S, P., W. A. Feder and King Tsue. Nutritional relation ships among certain filamentous fungi and a marine nematode. Science, 141, 520-522 (1963). 2. Meyers, S. P., W. A. Feder and King Tsue. Studies of relation ships among nematodes and filamentous fungi in the marine environment. Dev. Indus. Microbiol., 5, 354-364 (1964), 3. Altman, J. and King Tsue. Changes in plant growth with chemicals used as soil fumigants. Plant Disease Reporter, 49, 600-602 (1965) 4. Bunt, J. S. and C. C. Lae. Observations within and beneath antarctic sea ice in McMurdo Sound and the Weddell Sea. Methods and Data. XMAS Special Publ., Oniv. of Miami, 69-1 (1969). 5. Bunt, J. S. and C. C. Lee. Seasonal primary production within and beneath antarctic sea ice. J. Mar. Res., 2{3), 304-320 (1970) 6. Bunt, J. S., K. E. Cooksey, M. Heeb, C. C. Lee and B. F. Taylor. Algal nitrogen fixation in the marine subtropics: Assay by acetylene reduction. Nature, 227, 1163 (1970). 7. Corcoran, E. F. and C. C. Lee. The distribution of chlorinated hydrocarbon pesticides in tne Mississippi River Delta and the adjacent Gulf of Mexico. Joint Southeast and Southwest ACS Meeting, New Orleans (Abst.). Dec. 2-4, 1970. p. 88. 8. Taylor, D. L. and C. C. Lee. A new Cryptomonad from Antarctica: Crvotomonas cryophila sp. Nov. Archiv fur Mikrobiol., 75_, 269-23 0 9. Bunt, J. S., C. C. Lee and E. L. Lee. Primary productivity and related data from tropical and subtropical marine sediments. Mar. Biol., 16, 28-36 (1972). 10. Bunt, J. S. and C. C. Lee. Data on the composition and dark survival of four marine aicroalgae. Limnology and Oceanogr., 17, 458-461 (1972). 11. Fenchel, T. and C. C. Lee. Studies on ciliates associated with sea ice from Antarctica. I. The nature of the fauna. Archiv fur Protistenk., 114, 231-236 (1972). 12. C. C. Lee and T. Fenchel. Studies on ciliates associated with sea ice from Antarctica. II. Temperature responses and tolerances in comparison with ciliates from temperate and tropical habitats. Archiv fur Protistenk., 114, 237-244 (1972). HONS 207556 PUBLICATIONS CONT'D. 13. Bunt, J. S., C. C. Lee and M. A. Heeb. The site off Freeport intended for Hydro-Lab. Hydro Lab J., 1:3-6 (1972). 14. Bunt, J. S., C. C. Lee. An exploratory study of benthic primary production. Hydro Lab J. , 1_:7-10 (1972). 13. Taylor, B. F., C.0. Lee and J. S. Bunt. Nitrogen-fixation associated vith"the blue-green alga, Trichodesmium. as measured by the acetylene-reduction technique. Archiv fur Mikrcbiologie, 8:205-212 (1973). 16. C. C. Lee, E. L. Lee and J. S. Bunt. Distribution of bicrass In a corl reef transect. In: Results of the Tektite Program, coral reef invertebrates and plants (Sylvia Earle and Robert Lavenberg, editors). Natural History Museum, Los Angeles Count'/, Science Bull., 20:69-75 (1975). MOMS 207559 CURRICULUM VITAE July 1977 Theodore J. Kr.eip Date and Place of Birth: December 20, 1926 - St. Paul, Mir.r.esc Marital Status: Married, Six children Residence: 162 E, Fairntount Avenue Maywood, New Jersey 07607 Professional Address: New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5232 Title: Research Professor, Deputy Director Laboratory for Environmental Studies Education: 1950 - B. of Chemistry (with distinction) University of Minnesota 1952 - Master of Science University of Illinois 1954 - Doctor of Philosophy University of Illinois Positions Bald: New York University Medical Center Department of Environmental Medicine 1967 - present Senior Research Scientist 1967 - 1975 Adjunct Associate Professor 1969-1971 Adjunct Professor 1971-1975 Research Professor 1975 - Acting Director, Laboratory for Environmental Studies 1968-1970 Deputy Director, Laboratory for Environmental Studies 1970-present HONS 207560 Curriculum Vita* Pag* 2 Course Offerings: (Department of Environmental Medicine) Analytical Chemistry of Environmental Contaminants. Radiochenistry. Positions Held: (continued) Mallinckrodt Chemical Works St. Louis, Missouri 1954-1966 Research Chemist, Uranium Division 1954-1957 Group Leader, Uranium Division 1957-1959 Head, Analytical Research Laboratory 1959-1961 Asst. Manager, Analytical Laboratory, 1961-1963 Uranium Division Manager, Laboratory Products Research 1963-1966 Honor Societies: Phi Lambda Upsilon, 1950 Sigma Xi, 1954 Professional Societies: American Chemical Society American Association for the Advancement of Science American Public Health Association American Industrial Hygiene Association Air Pollution Control Association American Conference of Governmental Industrial Hygienists The New York Academy of Sciences Vic* Chair - Environmental Sciences Section - 1976 Chair - Environmental Sciences Section - 1977 * 79 Elected Fellow - 1977 HONS 207561 Curriculum Vitae Pag* 3 Committees: Consulting Chairman of Subcommittee t 6 Metals 1, Intersociety Conanittee on Manual of Methods for Ambient Air Sampling and Analysis 1971-1977 Member 1971-present Member of International Meeting on Effects and Dose-Response Relationships of Toxic Metals, Tokyo, November 13-23, 1974. Organized by: The Subcommittee on Toxicology of Metals of the Perr.ar.en Commission and International Associa on Occupational Health. Member of American Society for Testing and Materials Committee D-22: Methods of Sampling and Analysis of Atmospheres, Subcommittees D-22.04, 1973-Present. D-22.09, 1975-Present. New York State - Governor's ad hoc committee on PCS's, 1975. Secretariat, International Standards Organization/Technical Committee 146 (Air Quality)/Working Group 3 Mitrogen Compounds, 1975-1976. Secretariat, International Standards Organization/Technical Committee 146 (Air Quality)/Subcommittee 2 Workplace Atmospheres, 1976-present. Various corporate organizations including Consolidated Edison, RSR Corporation, Equitable Environmental Health. United States Department of Commerce, National Oceanic and Atmospheric Administration, Harm* Ecosystems Analysis Project, New York Bight Contaminants Panel. Cornell University, New York State College of Agriculture and Life Sciences and New York State College of Human Ecology, Cooperative Extension, New York City Urban Gardening Prcgra.-. - HONS 207562 |1 it Curnculua Vita* Pag* 4 List of Published Works: 1. Liitinen, a. A. and T, J. Kneio, "Polarographic and Coulore-r-c Study of p-Dinethvlanmofienzane," Journal of one Are-c*- Society, 71:736-740 (1956). =iI-=:----- 2. King, P. , E. Br*tz and T. J. Knelt. "Determination of lodir.a in. Mineral Pramlxes," Analytical Chemistry. 14:565-567 (1962). 3. Kneio, T. J., T. H. Seaslv, p. King and W. K. D*an. "Programmed Temperature Gas Chromatography with Dual Elactron Capture Detector for Pasticida Residua Analysis,* Analytical Chanistrv. 39:lsnn- 1512 (1967). ' ----------------------------- ~ 4. Cohan, N. and f- J. Kneln. "A Hathod for tha Analysis of 2-Pb in Unna of Uranium Minors," Journal Haalth Physics, 17: 125-130 (1969) . 5. Strohlow, C. D. and T. J. Kneio. "Tha Distribution of Load * and Zinc in tha Human Skeleton," Journal American Industrial Hygiene Association, 10:372-378 (l9<>9). _ 6. <n*in, T. J., G. P. Howells and M. E. Wrann. "Trace Metals, ntsxuncrrma and Pesticides in the Hudson River," Symposium on Hudson River Ecology, October 1969, Proceedings of -.ha Svaosu, Now York State Department of Environmental Conservation, Alaany, New York. 7. rurtado, V. C., t. J. Kneln and M. Eisenbud. "Measurer.ont of Low Levels of in Reactor Atmospheres," Nuclear Applications ar.d Technology, 9:268-273 (1970). .8 Knel^, T. J., M. Eisenbud, C. D. Strehlew and P. C. Freudenth&l. JLLruorne Particulates in No/ York City," Journal Air Polluticn Control Association, 20:144-149 (1970). 9. Howells, G. P.. T. J. Kneln and M. Eisenbud. "Water Quality in Industrial Areas: Profile of a River," Environmental Science ard Technology, 1:26-35 (1970). 10. *p. T- J- "Atomic Aioarption Spectrophotometry," AIHA Confer ence on Advances in Instrumental Method of Analyses, Environmental Trace Substances Center, University of Missouri, Columbia, Misss-r (May, 1970). MOMS 207563 Curriculum Vita* Pag* 5 11. Laurer. G. J- Knelt- a. E. Albert and F. s. Kent. *Ir. Sit. Determination of Lead on Pa-.nted Surfaces for the Prevention o; Childhood Lead Poisc.-mg,' Proceedings of t.-.g Third Svocsi.- c Low Enertv X- and Gar.-a Rev Soirres i-o. Aot-.cincrs. S;-- i: and Brearn Science Puclisnars, ..ew Yorx, go, 235-ill uTTTTT 12. Lentsch, J. W., M. E. Wrenn, T. J. Ere is ar.d M. Eisenbud. "Manmade Radionuclide* in the Hudson River Estuarv," Prrc, 5th Annual Midyear Teoical Symposium, Health Physics Society, Idaho Falls, Idano, p, 499 (1970). 13. Howells, G. P., M. Eisenbud ar.d T. J. Xneip. "Ecology of the Estuary of the Lower Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources ar.d Flsnino. Publ. FIR:.'12/7 0/-20, Food and Agriculture Organization o: tne United Nations, New York (1970). 14. Knelo. T. J. "Concentration Factors, Environmental Complexity and the Analytical Chemist," American Chemical Society,"Anaiyt Division--National dureau of Standards Summer Symposium: Key Progress in National Problem Areas, Washington, D. C. June 197 Proceedings of the Svmoosium uo - ' 15. T- T- - G. P. Howells and M. E. Wrenn. "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, Puol. fIR:H?/70~/E-2C. Food ana Agriculture Organization of the United Nations, New York (1970), 16. Laurer, G. R., T. J. Kneip, R. E. Albert and F. S. Kent. "X-ray Flourescence: Detection of Lead in Wall Paint," Science, 172'. 466-468 (1971). 17. Knelp, T. J., N. Cohen, D. H. Goldstein and E, A. S. Muchrore, "Experimental Studias of Lead Toxicity in Trace Substances in Environmental Health - V," Proceedings of University of Missouri's 5th Annual Conference on Trace Substances m Environmental Health (ed. by D. D. Hempnilli, pp. 349-361, < June IS 7 IT---------------- 18. Eisenbud, M. and T. J. Kneie. "Trace Metals in the Atmosphere," Tech. Paper #16, New York State Department of Environmental Con servation, Environmental Quality Research and Development Unit (July, 1971). 19. Lentsch, J. W.. T. J. K.neia, M. E. Wrenn, G. P. Howells and M. Eisenbud. "Stable Manganese and Manganese-54 Distributions m The Physical and Biological Components of the Hudson River xstiir/, Proc. Third National Svmnosiun on Hadioecologv, Oak Ridge (1371) , HONS 207564 Curriculum Vita* Pag* g 20. Kneip, T. J. "Sampling and Analytical Problems m a Complex Natural System: The Hudson River Estuary," American Laboratory 3_:47-52 (1971). 21. Cohen, N., T. J. Kneip, D. Goldstein and E.A.s. Muchmore, "The Juvenile Baboon as a Model for Studies of Lead Poisoning in Children," Journal of Medical Primatolccv 1: 142-155 (1972). " 22. Kneip, T. J. and G. R. Laurer. "Isotope Excited X-ray Fluorescence," Analytical Chemistry, 44:57A-6A (1972). 23. Laurer, G., M. Eisenbud, R. E. Albert, T. J. Kneip and N. Nelson. "New York City Health Research Council X-ray Fluorescence Analyzer for Lead," Isotopes and Radiation Technology 9^:275-277 (1972) . *24. Kneip. T. J., *t al., "Tentative Method of Analysis for Inorganic Lead Content of the Atmosphere," Health Laboratory Science 9^1):79-83 (1972). 25. Laurer, G. R., R. E. Albert, T. J. Kneip, B. Pasternack, C. Strehlow, N. Nelson and F. S. Kent, "The Distribution of Lead Paint in New York City Tenement Buildings, AJPH 63: 163-168 (1973). 26. Kneip, T. J. and G. J. Lauer. "Trace Metal Concentration Factors in Aquatic Ecosystems," iii Vol, 5, Progress in Analytical Chemistry, Plenum Press, New YorSTi New Vork (1973) . 27. Ahuja, S., E. M. Cohen, T. J. Kneip, J. L. Lambert, G. ziveig, eds. Chemical Analysis of the Environment and Other Modern TechniguesT Plenum Press, New York, New lork (1973). 28. ^9. Kleinman, M. T., T. J. Kneip and M. Eisenbud, "Meteorological Influences on Airborne Trace Metals and Suspended Particulates," Proceedings, 7th Annual Conference on Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June 12-14 (1973). Kneip. T. J., *t al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter," Health Laboratory Science 10_(3) :214-217 (1973) . *30. Kneio, T. J., et al., "Tentative Method of Analysis fcr Molybdenum Content of Atmospheric Particulate Matter by Atomic Absorption Spectrophotometry," Health Laboratory Science 10(3):218-220 (1973). "Reprinted in Methods of Air Sampling and Analysis, Second edition. Katz, M., *d. American Public Health Association w*hi nctor., D.C. (1977) . HONS 207565 Curriculum Vitae Page 7 31. Kneip. T. J., et al., "Tentative Method of Analysis for Nicxel Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10_(3 } : 221-225 (1973). " -------------- 32. Kneip, T, J., et al., "Tentative Method of Analysis for Copper Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3):226-230 (1973). -------------- 33. Kneio, T. J., et al., "Tentative Method of Analysis for Chromium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(4):358-361 (1973). 34. Kneip, T. J., G. P. Howells, M. E. Wrenn, "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," In Marine Pollution and Sea Life. Fishing News (Books) Ltd., Surrey, England, p. 169 (1973). (Reprinted from Ref. 15) 35. Kneip, T. J., M. T. Kleinman and M. Eisenbud, "Relative Contributions of Emission Sources to the Total Airborne Particulates in New York City," Proceedings, Third International Clean Air Congress, October 8-12, 1973 (1974). 36. Cohen, N.,T. J. Kneip, V. Rulon and D. Goldstein, "Biological and Toxicological Response of Infant Baboons to Lead Driers in Faint,' Env. Hlth. Perspectives, Experimental Issue #7, DHEW Pub. # NIH `74-213, O.S, Gov't Printing Office, Washington, D.C., p. 161 (1974), 37. Kneip, T. J., N. Cohen and V.' Rulon, "Potential Sources of Analytical Error in the Erythrocytic S-Aminolevulinic Acid Dehydratase Test for Lead Exposure," Analytical Chemistry. 46:1863-1866, (1974). 3* Bernstein, D. M., T. J. Kneip, M. T. Kleinman, R, Riddick and M. Eisenbud, "Uptake and Distribution of Airborne Trace Metals in Man," Proceedings of the 8th Annual Conference of Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June (1974). 39. Kneip, T. J., G. R* and T. Hernandez, "Cadmium in an ` Aquatic Ecosystem: Distribution and Effects," Trace Substances in Environmental Health - VIII, 1974. A Symposium! D. D. Hempnill, ed.. University of Missouri, Columbia (1974), HONS 207566 Curriculum Vitae Page 8 40. Bondietti, E. A,, F, H. Sweeton, T. Tamura, R. M. Perhac , L. D. Nulett, and T. J. Kneip, "Characterization of Cadmium and Nickel Contaminated Sediments from Foundry Cove, New York," In_; Proceedings of the First Annual NSF-RANN Trace Contaminants Conference, Oak Ridge, Tennessee, August, 1973. pp~7 311-224. CQNF-730302^ ORNL, Oak Ridge, Tennessee (T974), *41. Kneig, T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2):112-116 (1974). -------------- *42. Kneip, T. J., at al., "Tentative Method of Analysis for Iron Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 118-121 (1974). *43. Kneip, T. J., et al., "Tentative Method of Analysis for Lead Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2) : 122-127 (1974). *44. Kneip, T. J., et al., "Tentative Method of Analysis for Manganese Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2) :128-133 (1974). *45. Kneip, T, J., et al., "Tentative Method of Analysis for Zinc Content ^Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 134-139 (1974). *46. Kneip, T. J., et al., "Tentative Method of Analysis for Vanadium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(3)-.240-245 (1974).* *47. Kneip, T. J., et al. Tentative Method of Analysis for Elemental Mercury in Ambient Air by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 11(4)-.342-346 (1974). HONS 207567 Curriculum Vitae Page 9 *48, 5Si.' T- J*' M- i-seni3U^' M. T* Kleinman and 0, M. Bernsteir., "Studies of Trace Substances in an Urban Atmosphere," Proceedings of the International SymDosium of Recent Advances In me Assessment of the Heaitn Effects or Environmental PollutionT Pans, June lii-28. 1974 (X975) . *49. Goldstein, D. H. , T. J. Kneip, V. Rulon and N. Cohen, "Erythrocytic 6-Aminolevulinic Acid Dehydratase (5-ALAD) Activity as a Biological Parameter for Determining Exposures to Lead," Journal of Occupational Medicine, 17:157-162 (1375). 50. Kneip, T J., "Potential Applications of X-ray Analytical ' Method in Paint Analysis," Journal of Paint Technology, 47_: 71-76 (1975). 51. unelp, T. j., T. Hernandez and Gary Hi, "Cadmium in an Aquatic Ecosystem: Transport and Distribution," In: Trace Contaminants in the Environment," Proceedings of the Second Annual NSF-RANW Trace Contaminants Conference, Asilomar, Pacific Grove, California, 29-31 August, 1974 (1975). 52. Kneip, T. J., et al., "Tentative Method of Analysis for Elemental Mercury in the Working Environment by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 12^2) -.158-162 (1975) . 53. Kneip, T. J., et al., "Lead in Blood and Urine," Health Laboratory Science 12_(4) :375-378 (1975) . 54. Kneip, T. J., et al., "Mercury in Urine," Health Laboratory Science 12(4)>379-382 (1975). 55. Kneip, T, j., et al., "General Atomic Absorption Procedure cor Trace Metals in Airborne Material Collected on Membrane Filters," Health Laboratory Science 12(4)i383--393 (1975). 56. Kneio, T.J., N. Cohen, V. Rulon, David Goldstein and ft. 'Ueison, "Factors Influencing Dose-Response Relationships for Toxic Metals," In: Effects and Dome-Response Relationships of Toxic Metals, G.F. Nordberg, ed., Associated Scientific Publishers, Amsterdam (1976). 57.Eisenbud, M., T. J. Kneip, M. T. Kleinman, D. M. Bernstein, "Trace Metals in Urban Aerosols," Final Report to Electric Power Research Institute, October, 1975. NTIS Ph-248-324 (1976) . HONS 207560 Curriculum Vitae Page 10 58. Bernstein, 0. M., M. T. Kleinman, T. J. Kneig, T. L. Chan and M, Lippmann, "A High-Volume Sampler for the Determinant of Particle Size Distributions in Ambient Air," Journal of the American Air Pollution Control Association 26(11), 1767^1072 (197 5):----------------------------------------------------------------------- 5* Kleinman, M. T., T. J. Kntip and M. Eisenbud, "Seasonal Patterns of Airborne Particulate Concentrations in New Yor.< City," Atmospheric Environment 10_:9-11 (19*6). Kleinman, M. T., D. M. Bernstein and T, J. Kneip, "An Apparent Effect of the Oil Embargo on Total Suspended Particulate Matter and Vanadium of New fork City Air," J. Air Poll. Con. Assoc. 22:65-67 (1977). 61. Kneip, T.J., R. Peter Mallon and Virginia P. Rulon, * "The* Effects of Anemia on Heme Synthesis Parameters During Lead Exposure," American Industrial Hygiene Association Journal 37, 578 -585 (1977). 62. Kneip, T. J., et al., "Analytical Method for Antimony in Air and Urine," Inj Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American public Health * Association, Washington, D. C., pp. 765-769 (1977). 63. Knei, T. J., et al., "Analytical Method for Arsenic in Urine and Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health ` Association, Washington, D. C., pp. 769-773 (1977). 64. Kneip, T. J., et al., "Analytical Method for Arsenic, Selenium and Antimony in Urine and Air by Hydride Generation and Atomic Absorption Spectroscopy," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 774-780 (1977). 65. Kneip, ?. J., et al., "Analytical Method for Chromic Acid Hist in Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 785-788 (1977). 66. Kneip, T. J., et al., "Analytical Method for Lead in Air," in: Methods of Air Sampling and Analysis, Second edition, KatzTM.-, ed. American Public Health Association, Washington, D. C., pp. 808-818 (1977). 67. Hazen, R, E. and T. J. Kneip. "The Distribution of Cadmium ' in the Sediments of FoundryCove," In: Hudson River Scalocy, Proceeding of a Symposium, Hudson River Environmental society, ed. HajcnTI-Jfl, 1976 (1977). HONS 207569 Curriculum Vita* Pag* 11 68. Kneip, T. J., M. T. Kleinman, R. Riddick and D. M. Bernstein, "Trace Elements in Human Tissues," Proceedings off the International Workshop on Biological Specimen Collections, April 13-22 ,_ 1977, iuxsmbourg, (In press, 19 77 ; . 69. Kneip, T. J., "Effects of Cadmium in an Aquatic Environment," In: Cadmium 77, Edited Proceedings First International Cadriur Conference, San Francisco, 'il Jan.-2 Feb., 1977. Metal Bulletin Limited, London, pp. 120-124, (1978). 70. Dais*y, J. M. , I. Hawryluk, T. J. Kn*ip and F. Mukai, "Mutagenic Activity in Organic Fractions of Airborne Particulate Matter," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 71. Kneip, T. J., M. A. Leyko, M. T. Kleinman, M. Lippmann and * J. M. Daisey, "Organic Matter in New York City TSP: Comparisons of Seasonal Variations and Relationships to Source Tracers," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 72. Lioy, P. J., G. T. Wolff and T. J. Kneip, "Toxic Airborne Elements in the New York Metropolitan Area," J. Air Poll. Con. Assn., 28 (5) :510-512 (1978). 73. Leaderer, B. P., 0. M. Bernstein, J. M. Daisey, M. T, Kleinman, T. J. Kneip, E. 0. Knutson, M. Lippmann, p. j. Lioy, K. A. Rahn, D. Sinclair, R. L. Tanner, and G. T. Wolff, "Summary of the New York summer Aerosol Study (NYSA5)," J. Air Poll. Con. Assn., 2(4):321-327 (1978). 74. Fennikoh, K. B., H. I. Hirshfield and T. J. Kneip, "Cadmium Toxicity in Planktonic Organisms of a Freshwater web," Environ. Res. (In press, June, 1973). 75. Xneip, T. J. and M. T. Kleinman, "Sampling and Multielement Analysis of Ambient Aerosols," In: Special Environmental Report No. 10, Air Pollution Measurement Techniques, Proceedings of the WHO Air Pollution Measurement Techniques Conference (APOMET), Gothenburg, 11-15 October, 1976. World Meteorological Organization, Geneva, Switzerland (1977). 76. Laurer, G. R. and T. J. Kneip, "Portable X-ray Units for Analyzing Lead in Paint," In: A Report of the Committee on Environmental Improvement: Assessing the Problem of Lead-Based Paint "Hazards pp. 13-17, American Chemical Society, Wasnington, D.C. (August, 1978). HONS 207570 PERSONAL EDUCATION HONORS AWARDS SOCIETIES Am, ILLATIONS CURRICULUM VITAE JOSEPH MARTIN O'CONNOR Born: November 19, 1944, Boston, Massachusetts Married: 2 children Bachelor of Science, Biology June, 1966 Collage of the Holy Cross Worcester, Massachusetts (Philosophy minor) * Doctor of Philosophy, Eeology/Physiology November, 1971 State University of New York at Albany Albany, New York Dissertation Title: Photoperiodic control of pituitary gonadotropin release in trout (Salmo gairdnen Richardson' and Salvelinus fontinalis (Mitchell) and in the leopard frog (Rana pioiens Shreber) Sigma XI Beta Beta Beta Biological Society Student representative to Faculty Senate State University of New York at Albany R.E.S.A. (Sigma Xi) Research Grant-in-Aid N.S.F. Pre-doetoral fellowship (Honorable) Research Grant-in-Aid, Edmund Niles Hyuck Preset Fellowship, Research Foundation of New York Stst New York Academy of Sciences American Society of Limnology and Oceanography Estuarine Research Federation Atlantic Estuarine Research Society AAAS American Society of Natural History Ecological Society of America American Fisheries Society Sigma Xi Great Lakes Research Society Florida Defenders of the Environment Board of Consultants, Lawler, Matusky and Skelly Engineers, Tappan, New York HONS 207571 experience present 1974-75 1973-74 1970-73 1967-70 1965-67 (summers) PROFESSIONAL ACTIVITY Research Assistant Professor Department of Environmental Medicine, and Assistant Director Laboratory for Environmental Studies New York University Medical Center Sterling Forest, Tuxedo, New York Senior Biologist Office of Professional and Technological Development Lawler, Matusky and Skelly Engineers 415 Rt. 303, Tappan, New York Director Biological Studies Section and Laboratory Office of Research and Technology Quirk, Lawler and Matusky Engineers and ' QLM Laboratories, Inc. Tappan, New York Research Associate University of Maryland Natural Resources Institute Hallowing Point Field Station prince Frederick, Maryland Graduate Research Fellow Dept, of Biological Sciences State University of New York at Albany Faculty Research Assistant University of Maryland Natural Resources Institute Chesapeake Biological Laboratory Solomons, Maryland Member, Advisory Committee Chesapeake Bay R.A.N.N. program of the National Science Foundation 1971-1972 U.S. Dept, of Agriculture Graduate School programs in Aquatic Ecology and Pollution Biology Counselor Rockland County Guidance Center for Women Guidance in Career Programs Adjunct Assistant Professor Inst, of Mar. and Atmos. Sciences of the City University of New York HONS 207572 3 Advisor, Fisheries Biology U.S. Dept, of Commerce National Oceanic and Atmospheric Adamistrati-England Offshore Mining Environmental sticy Visiting Investigator University of Delaware College of Marine Studies Bayside Laboratory Lewes, Delaware 1972 RESEARCH INTERESTS Population dynamics Community dynamics Respiratory physiology of fishes Reproductive physiology of fishes Chemical-biological interrelationsnips m ecology Ethology of fishes TEACHING INTERESTS physiological ecology General physiology General ecology Pollution biology Ichthyology PUBLICATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of frog ovarian oocytes. Amer. Zool. 9:??9. O'Connor, J.M. and R.G. Strose. 1970. photoperiodic control of the pituitary gonadotropin release patter of the frog, Rana pipiens Shreber. Abstr. Proc. N.E. Division Comp. Physiology section, Amer. Soc. Zool. O'Connor, J.M. 1970. The effects of thermal shock on the tidal activity patterns of the hogchoker, Trlnectes maculatus. Abstr. Amer. Society of Ichthyologists and Herpetologists, Ann. Mtg., New Orleans, La. O'Connor, J.M. 1971. photoperiodic control of the pituitary gonadotropin release patterns in the rainbow trout (Salmo gairdneri (Rich.,)), the brook trout (Salvelinus fontinalis MitchellT end in the leopard frog (Rana pipiens Shreber). Ph.D. Thesis. Library, State University of New York at Albany, and University Microfilms, Ann Arbor, Mich. HONS 207573 4 O'Connor, J.M. 1972. Pituitary gonadotropin ralaase patterns in rainbow trout (SalTio gairdnen) brook trout (Salvelmus fontinalis) and in the leopard frog (Rana pipiens). ConoT Biocnem. Physiol. 43(A):739-746 . ' O'Connor, J.M. 1972. Tidal rhythm in the activity patterns of the hogchoker, Trmectes maculatus (Bloch and Schneider). Exp. Mar. Biol. Ecol. 9:173-170. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1972. The effects of suspended and deposited sediments on estuarine organisms. Univ. Md. N.R.I. ref. 72-9. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1973. The effects of suspended and deposited sediments.on estuarine organisms. Univ. Md. N.R.I. ref. no. 73-4. O'Connor, J.M., R.W. Williams and J. Converse. 1974. Studies of fish impingement at the Nine Mile Point Nuclear Station, Lake Ontario. Abstr. 17th Conference on Great Lakes Re search. Int'l Assn, for Great Lakes Research. O'Connor, J.M. and J.A. Sherk. 1974. The responses of sosie estuarine organisms to concentrations of suspended solids, proc. of the Seventh Dredging Seminar, Ctr. for Dredging Studies, Texas Aam University. Sherk, J.A., J.M. O'Connor and D. Neumann. 1975. The effects of suspended sediments on estuarine environments. IN: L.s. Cronin (Ed.). Estuarine Research, Vol. II. Geology and Engineering, Academic Press, N.Y. pp. 541-557. Neumann, D.A., J.M. O'Connor and J.A. Sherk. 1975. Respiratory and hematological responses of the oyster toadfish (Opsanus tau) to suspended sediments. Trans. Amer. Fish. Soc. ITT: 775-781. Sherk, J.A. and J.M. O'Connor. 1976. Effects of sedimentation on coastal zone organisms. Proc. Ill Federal Inter-Agency Sedimentation Conference. O'Connor, J.M. and S. Schaffer. 1977. Effects of sampling gear on striped bass ichthyoplankton. Chesapeake Science 18:312-315. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1976. Effects of suspended solids on selected estuarine plankton. U.S. Army Corps of Engineers Coastal Engineering Research Center. Misc. Rept. 76-1. 50 pp. Jan. 1976. HONS 207574 O'Connor, J.M., D.A. Neumann, and J.A. Shark, Jr. 1976. Lethal affects of suspended sediments on estuarine fish. Technical Paper No. 76-20, U.S. Army, Coastal Engineering Research Center, 38 pp. O'Connor, J.M., D.A. Neumann, and J.A. Shark. 1977. Sublethal effects of suspended sediments on estuarine fish. Technical Paper No. 77-3, U.S. Army, Coastal Engineering Research Center, 90 pp. Rachlin, J. , A.P. Beck, and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass, Morone saxatilis * (Walbaum) . Copeia 1978(2):334-336. Ginn, T.C. and J.M. O'Connor. 1978. Responses of the estu arine amphipod Gammarus daiberl to chlorinated power plant effluent. Estuarine Coastal Mar. Sci. 6:459-469. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1978. An opera tional system for simulating the physical and chemical en vironment of the condenser tubes of a power plant. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). Zubarik, L.S., and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). O'Connor, J.M., C.C. Lee, and M. Eisenbud. Environmental Health. M.E.S.A. New York Bight Atlas Monograph 32 (in press). Ginn, T.C., G.V. Poje, and J.M. O'Connor. 1978. Survival of planktonic organisms following passage through a simu lated power plant condenser tube. Proc. Fourth Nat'l Workshop on Entrainment and Impingement, pp. 91-101. PUBLICATIONS IN REVIEW O'Connor, J.M., D.A. Neumann and J.A. Shark. The responses of estuarine fishes to highly turbid environments. Water Res. O'Connor, J.M. and J.A. Shark. The effects of suspended moral solids on the white perch, Morone americana. Trass. Amor. Pish. Soc. O'Connor, J.M. and J.A. Mihursky. The effects of thermal shock on the activity patterns of the hogchoker, Trmectes maculatus. Ches. Sci. HONS 207575 6 MANUSCRIPTS IN PREPARATION Eichorn, K.L. , T.C. Ginn and J.M. O'Connor. Response of striped bass (morone saxatilis) to chlorinated cooling water. O'Connor, J.M., G.V. Poje, and S. Rigdon. Respiration of striped bass (Morone saxatilis) eggs and larvae. Becker, A.J. and J.M. O'Connor. Aerobic shutdown in the green crab (Carcinus maenas) in response to temperature, salinity and sulfide. Steinhausler, F., S. Schaffer, N. Cohen, c. Lee, J. O'Connor and M. Wrenn. Effect of high LET radiation on intracellular ATP content of prokaryotic and eukaryotic algae. Hendrick, E., J.M. O'Connor, and G.R. Laurer. A method for analysis of trace mineral content of fish otoliths using X-ray fluorescence spectroscopy. PRESENTATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of ovarian oocytes in the frog, Rana pi plans. 1 Amar. Soc. Zool. O'Connor, J.M. and R.G. Stross. 1969. Photoperiodie control of the pattern of pituitary gonadotropin release in the frog Rana plplens Shreber. Amer. Soc. Zool. O'Connor, J.M. 1966. Alterations in the tidal activity rhythm in the hogchoker after exposure to thermal shock. Atlantic Estuarine Research Society, Cambridge, Md. October, 1966. O'Connor, J.M. and J.A. Shark. 1971. The lethal effects of suspended solids on estuarine fishes. Joint Mtg. Atlantic Estuarine Research Society and the Estuarine Research Federation. Pleasantville, N.Y. October, 1971. Neumann, O.A., J.M. O'Connor and J. Shark. 1972. Studies of the respiration of the striped bass and the white perch exposed to suspended sediments. Atlantic Estuarine Research See. Ocean City, Md. April, 1972. O'Connor, J.M., J.A. Shark and D. Neumann. 1973. "Inert" mineral compounds as potential factors in mortality and toxin transport in estuarine organisms. E.P.A. Workshop on Chemical Pathogens in the Environment. Pensacola, Fla., May, 1973. MONS 207576 Shark, J.A., J.M. O'Connor and d. Neumann. 1973. Tha effects of suspended solids on estuarina environments. Symp. on " Recant Advancas in Estuarine Research. It int'l. Conference of tha Estuarine Research Federation. Myrtle Beach, S.c. October, 1973. ` O'Connor, J.M., and J.A. Shark. 1974. The responses of some estuarina organisms to concentrations of suspended solids. Seventh Dredging Seminar. Sp. by tha Center for Dredging Studies, Texas AiM University, New Orleans, La. November, 1974. O'Connor, J.M., R. williams and J. Converse. 1974. Impinge ment studies at the nine mile point nuclear power station, Lake Ontario. 17th Conference on Great Lakes Research, August, 1974. Hamilton, Ontario, Canada. O'Connor, J.M., and S.G. O'Connor. 1975. Seasonal Compartments ration of primary productivity in the New York Bight, 1973-74. Am. Soc. of Limnology and Oceanography, Halifax, N.S., Canada, June, 1975. O'Connor, J.M. 1976. Assessment of entrainment impact at the Indian Point Nuclear Station. Third National Work shop on Entrainment and Impingement, New York city. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigrlnus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society. May 1976. ` Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatills and the amphipod Gammarus to chlorinated power plant effluent. Northeast Fish and Wild life Conference. April 26-29, Hershey, Pennsylvania. Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrain ment effects. Northeast Pish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1977. An operational system for simulating the physical and chemical environment of the condenser tubes of a power plant. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. ^SBbmrik, 1.3., and J.M. O'Connor. 1977. A radioisotopic study mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. HONS 207577 s O'Connor, J.M.,C.C. Coutant, and J.P. Lawler. 1977. Environ mental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. O'Connor, J.M., T.C. Ginn, and G.v. Poje. 1978. Survival of planktonic organisms following passage through a simulated power plant condenser tube. Fourth Nat'l Workshop on En trainment and Impingement. Chicago, Illinois. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor, and M. Wrenn. 1978. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radiation Research Society. Toronto, Ontario, Canada. HONS 207576