Document V37ZVQxvjd5denJ9nZa5mbXRK

AR 226 - 0BFS5 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-85, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixtureof 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyitraizole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect toxicity of the fluorochemical componenoft the test sample. METHOD: Method: OECD Guideline 201 and EPA CFR 40 797.1050. Type: Acute Static GLP: No Year study performed: 1996 Species: Selenastrum capricomutum Source: In-house cultures. Originally stock from ATCC, Rockville, MD Element basis: Algal cell counts (cells/ml), specific growth rates. Exposure period: 96 hours Statistical Methods: Not applicable due to study results. Analytical monitoring: Not given. Test Conditions: Algal Nutrient Medium: Nutrient medium for freshwater algae per OECD method. The pHofthis mediumwas 7.9. Stockand test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 500 mL of standard algal nutrient medium. Aliquots were then added directly to dilution water in test vessels to create test solutions. Exposure vessels: Not given Agitation: Not given : Numberof replicates: Not given Initial cell loading: 1x 10* cells/mL. Number of concentrations: one plus a blank control Lighting: Continuous Water Chemistry: Not given. RESULTS 002825 Nominal concentrations: Blank control and 1000 mg/L. Algal Growth Response E.Cso values Exposure | Mean Percent Change in (Contact) | Specific Growth Rate EC50 (mgfL) Hours (1000 mg/L Conc.) [2a 15 | >i00 | [e er T 51000 | [Cee [47 >f00 Element values are based on nominal concentrations. Remarks: Testing was conducted on the mixtuarse described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS FC-3017 exhibits a 96-hour ECso for specific growth rate of >1000 mg. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY SRetluidaybillaictyk:s dKelsicmriispcthiornanaknidngre=co4r.dsNoofttahllelmabeotrhaotdorfyoldlaotwaedc.oulSdambpelleocpautreidt.y t`whaesanmootupnrtoopferfllyuocrhoacrhaectmeirciazledpraonpodrtthioensitnutdhyelsaoclkustiaonna.lytical confirmation of REFERENCES This test was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N3188, 1996. OTHER Last changed: 6/26/00 : 002826 05/30/2000 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT 1 LAB REQUEST NO. N3188 REQUESTOR NAME: SBS/BOB COX DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 09/20/1995 DESC: FC-3017 (LIGHT WATER) CONTRACT LAB(S): ASCI EXP COMP DATE: 11/22/1995 DATE COMPLETED: 05/23/1996 PROJECT LEAD: SA BEACH PHONE NO: 651-778-7452 aM FAX NO: 651-778-6176 Standard set of data as quickly tests. Please make as possible without this a priority project as Europe needs incurring additional costs. Please also copy Agnes Vidal and Rene Lehenaff on testing results. MSDS Doc.# 10-3022-1. BOD and COD will be put on another lab request. sab 9/28/95 Results from Test Conc. ng/1 125 250 500 1000 ECS0 95% CI sab 9/28/95 Microtox Test: Percent Semin exp. 6.5 16 26 a4 > 1000 (+) Light Loss 15-min exp. 9.5 22 a7 57 780 (730 - 850) 30-min exp. 14" 28 a6 67 560 (540 - 570) Results from 96-hr Fathead Minnow Toxicity Test: Test was run as a static-renewal. Beakers were renewed at 48 and 72-hrs of exposure. This below the required was 60% done because the dissolved oxygen of saturation. (1000 mg/L renewed levels dropped at both times, 100 mg/L Test renewed onlyPaetrce7n2t hMouorrst.alitsyab 10/27/95 Conc., mg/L 24-hrs exp. 48-hrs exp. 72-hrs exp. 96-hrs exp. 1.0 10 0 0 0 0 0 10 10 10 100 1000 0 0 0 0 0 0 0 0 Duplicate 1000 0 eso >1000 0 >1000 0 >1000 0 >1000 NOTE: The blank controls and 1000 mg/L concentrations test combined a range finder with a definitive test at were 1000 duplicated; mg/L. this sab 10/27/95 Results from Activated Sludge Respiration Inhibition Test: Test Conc. mg/L 30-min Percent Exp. Inhibition3-hirn RExeps.piration Rate 1000 0 Dup. 1000 0 0 0 o- ECS0 > 1000 > 1000 N3o-hosuirgsnifoficaenxtposiunrheibittoio1n000inmgr/eLs.piration rate was seen after 30-minutes and sab 10/17/95 002827 BOD AND COD ARE ON LR N3688. sab 11/27/95 Results from algae toxicity test: Test Conc. mg/L 1000 Mean Percent change in Specific Growth Rate 24-hrs 48-hrs 72-hrs 96-hrs 15 9.1 16 17 EC50 >1000 >1000 >1000 >1000 sab 1/16/96 Results from Daphnia magna acute toxicity test: Test Cone. mg/L 1000 (all 4 reps) Percent Immobilization 24-hrs Exp. 0 48-hrs Exp. 0 EC50 sab 5/22/96 >1000 >1000 ~ SAMPLE DATE CODE DESCRIPTION RESULT * = CONTRACT LAB DATA 05/30/2000 LAB REQUEST NO. N3188 SAMPLE DATE CODE ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT CONTINUED DESCRIPTION RESULT 1 FC-3017 Amber Liquid ASCI 48-HR-EC50 *DAPHNIA ACUTE TEST 96-HR-LC50 FISH ACUTE TEST - 96 HOUR 1C50-ALGAE ALGAE ASSAY - IC50 MICROTOX MICROTOX - EC50 SLUDG-RESP SLUDGE RESPIRATION - ECS0 >1000 MG/L >1000 MG/L >1000 MG/L 560 MG/L >1000 MG/L MOL or (95% C.1.) 2 MDL or (95% C.I.) -) (-) (540 - 570) ") 002828 BEST COPY AVAILABLE iE _+OATEIOA AS. | --laddaloit go. -- = Bl_FC2aAF Amverhmuoa liquid HTet5 SHgiHanolffamily = 700000 Mii= scibfbuiulatey 7 Emi i af Diandan] Algal Nutdeat Mediu wa Zepaned; TERE ARG, + Eo Inoculaud i 200 vanesd_oferece alleyCoun LuRHAl dayJolord coouA ltueSatGR (kA as Hs wan Lo ToT sfeal = Bh Tna 1 ANP 5 AQ TS TR LR pA\OT RESTS X10]We aA SA 29{872.33/52 TOeEsCtDme#t2h0o1dolog"Algay cGornolowrtmhsIwinlhhtheiTfeobsltoi"waitnngdiFtweoodt:enRsetqpr:otVoolc.ol4s0. NoRTMR 797.1050 a *AlGaY AGiIe ToxcRT Ea sai oh Aba $s RRA LED 1A < ri _ TT a BEST COPY AVAILABLE STANDARD NEUNTVRIIREONNTMMEENDTIAULMLFAOBRORFRAETSOHRWYATER A: LGAE woTRENT INCOSNTCOECNKSTORLAUTTIIOONN FINATLECSONTCSEONLTURTAITOINON STOCK SOLUTION 1: MACRONUTRIENTS wor MgCl SHO CaCl 24,0 MgSO, THO KH,PO, STooKSOLUT2: FIeEODNTA 1500 12 ot 18 ot 15 ot 0.1601 15 mor 12 mot 18 mot 15 mot. 168ml. NFoacOhT6A4020 1a00 mnoott 1a00 vwott STOCKSOLUTION3: TRACE ELEMENTS Lnaawriss NNaaztso1d Emel Gol LTE H,80, 185 mot. 185 wo 2cMnCl" 4H,0 a415 mmoott 3415 ug CuNCCaolsCoo26r4H,02010 2 01051 mmoott. 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