Document RqJBYnOVkB6mXmobnLyjyNqV

</ ISTITUTO DI RICERCHE FARMACOLOGICHE "MARIO NEGRI ,, FONDAZIONE PER RICERCHE ERETTA in ente Morale con d. p. r, 361 DEL > APRILS 1961 . REG. PERSON'S GIUR. TRIB. MILANO N. 16}. VOL. J CONTO CORRENTB POST. N' 3/39071 RECOGNIZED AS A tax exempt setorganization under section (c) (1) of the united states of AMERICA INTERNAL REVENUE CODE CONFIDENTIAL ETHYLENE DICHLORIDE Report on the clinical chemistry results, after 6 months inhalatory exposure Second Report Milan, December 1976 20157 MILANO Via liritren, 02 Tel. 35.54.546 J5.70.J46 Telex 37268 NEGRINST SL 031236 2 Results obtained can be summarized as follows: BUN -(Table 1 and 2) . In female rats, a significant decrease in mean BUN levels in respect to controls was found in groups 1,2 and 3 , i.e. those exposed to the lower ECD levels, whereas in males a significant decrease was present only in group 1 although a tendency towards a decrease was present also in groups 2 and 3. No changes were found in groups 4 (i.e. 250 ppm later to 150 ppm) in either sex. The biological significancy of this type of results is probably doubtful. Serum Glucose - (Tables 3,4). No significant differences in respect to controls were found either in males or females exposed to ECD at any dosage level. Serum bilirubin - (Tables 5,6) . No statistically significant differences were found in both sexes between control and exposed rats, although a tendency towards a dose-related increase was present , more evident in females. Serum total proteins - (Tables 7,8). No statistically significant differences were found between control and exposed rats of either sex. Serum albumin - (Tables 9 , 10). No statistically significant differences were found between control and ECD-exposed rats of either sex. Serum GOT-levels - (Tables 11,12). No statistically signifies differences were found between control and exposed rats of either sex. 5L 031237 3 Serum LDH - (Tablesl3,14) . In female rats a tendency toward an increase in levels was present in all exposed groups, this increase showing however no dose relationship and reaching statistical significance only in group 3. Levels were slightly elevated in comparison to controls also in ECD-exposed males, again however no clear dose-response relationship was present and in no group the difference reached statistical significance. Serum cholesterol - (Tables 15,16). In female exposed rats the values were superimposable to those of controls; in males a slight tendency towards an increase was seen in all groups, higher in groups 1 and 2 (i.e. ECD levels of 5 and 10 ppm respectively). However no statistical significance was reached in any group. Uric acid serum levels - (Tables 17,18) . In both males and female a tendency towards an increase in levels was observed which did not show a clear dose-relationship in males nor reached stati stical significance in either sex or group. Alkaline phosphatase - (Tables 19,20). In females a tendency towards a decrease showing a roughly inverse dose-relationship was present but the differences did not reach statistical signifi cance in any group. In male rats, the values were very scattered with a profound decrease in group 1 (ECD 5 ppm) and lower decreases in groups 2 and 4; no differences with controls were seen in group 3. Si 031238 4 Albumin percent levels - (Tables 21,22) . No statistically significant differences were found either in females or males. Alpha-1 globulins - (Tables 23,24). No statistically significant differences were found between controls and exposed rats. Alpha-2 globulins - (Tables 25,27). A tendency towards a dose-relazed increase was seen in females n0t reaching statistical significance. In males a not significant increase was seen only in groups 2 and 4. Beta globulins -(Tables 27,28) No significant differences were seen. Gamma globulins - (Tables 29,30) No differences in females; a slight , not significant, not dose-related tendency towards an increase in male rats. ++ + " Na , K , Ca , Cl , inorganic P - (Tables 31-40) No statistically significant differences were observed. Leukocyte numbers - (Tables 41 42). A slight, not significant decrease was seen in group 4 animals both males and females; a higher decrease was however present in group 1 of male rats. Hematocrit - (Tables 43,44) . No significant differences in either sex , the tendency towards an increase (not dose related, not significant) present in females being absent in males. SL 031239 5 Hemoglobin content (Tables 45,46). No significant difference Erythrocytes numbers - (tables 47,48) . No significant differences. Mean erythrocyte volume -(Tables 49,50). A tendency towards a decrease (not significant; not dose-related) was found in males. Platelet number (Tables 51,52). No differences in females, a tendency (not statistically significant) towards a decrease in male rats exposed to 250-150 ppm ECD was on the other hand seen. Basophilic and eosinophilic leukocytes - (Tables 53-56). No significant differences. Segmented and non segmented neutrophilic leukocytes (Tables 57-60). Contrasting tendencies between males and females exposed to ECD, in any case no significant differences were detected. No differences for non-segmented neutrophils . Lymphocytes (Tables 61, 62) . A slight, not dose-related, not significant decrease in females, the opposite in males again not significant. Monocytes - (Tables 63,64). No significant differences. 03^" s^ 6 Urinary erythrocytes and glucose - (Tables 65,66;69,70). No significant differences. Urinary albumin - (Tables 67,68). A tendency towards an increase in animals exposed to the higher ECD dose (250-150 ppm) was seen; the semiquantitative assay does not permit statistical analysis. Urinary ketones, bilirubin, casts (Tables 71,76). No differences between exposed and controls. Urinary leukocytes, crystals, mucus, epithelial cells and microorganisms (Tables 77-86). No obvious differences between exposed and ECD-exposed animals could be-detected. COMMENT : On the basis of the data so far obtained, the ECD treatments employed would seem to be reasonably tolerated by the animals. Although changes in some of the parameters investigated (LDH, SGOT, Cholesterol, Bilirubin, Uric acid levels) were seen, in no case these differences reached statistica significance nor was a dose-response relationship clearly evident; accordingly the biological significance of these results for a possible involvment of the liver and the kidneys, is at the moment very uncertain. SL 031241 I* T7iBLE 1 - BUN values in control and ECD-exposed in female rats. CONTROL Rat No. rrg 811 812 813 814 815 816 817 818 20 23 16 23 25 11 21 18 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 15 15 13 12 12 18 14 9 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 16 15 15 15 16 15 11 17 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 15 12 16 12 17 22 16 16 GROUP 4 Rat No. ing% 9 21 10 20 11 18 12 20 13 17 14 18 15 20 16 16 X S.E. 19.6 2 1.60 13.50 0.94 Statistical analysis : Group C vs 1 : p <0.001 J| C vs 2 : p <0.005 C vs 3 : p < 0.05 " 1 vs 4 : p < 0.005 " 2 vs 4 : p Z0.05 15.00 0.62 15.75 1.11 18.75 0.62 SL 031242 TABLE 2 - BUN values in control and ECD-exposed male rats. e t CONTROL Rat No. mg% 854 855 856 857 858 859 860 861 19 18 19 15 17 15 20 21 GROUP Rat No. 1 mg% 654 10 655 14 656 14 657 12 658 10 659 12 660 9 661 13 GROUP 2 Rat No. mg% 4 54 25 455 12 456 14 457 16 458 17 459 16 461 13 462 14 GROUP 3 Rat No. mg% 254 15 255 16 256 15 257 16 258 13 259 17 260 17 261 19 GROUP 4 Rat No. mg% 54 15 55 19 56 22 57 18 58 15 59 18 60 16 61 17 S.E. 18.00 0.78 11.75 0.67 15.87 1.43 Statistical analysis : Group C vs 1 : p <0.001 II 1 vs 2 : p < 0.05 II 1 vs 3 : p <0.05 16.00 0.63 17.50 O TABLE 3 - Serum glucose in control and ECD exposed female rats. V 7 Z IE 0 CONTROL Rat No. mg% 811 90 812 95 813 75 V* 814 110 815 80 816 60 817 100 818 125 GROUP 1 Rat No. mg% 609 95 610 80 611 105 612 145 613 75 614 85 615 95 616 125 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 130 105 105 90 80 85 90 120 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 115 85 110 115 85 100 140 95 GROUP 4 Rat No. mg% 9 115 10 85 11 95 12 125 13 100 14 95 15 loo 16 80 X S.E. 91.87 7.25 100.00 8.18 101.25 6.53 No statistically significant differences were found between any group. 105.60 6.51 99.37 5.21 TABLE 4 - Serum glucose in control and ECD-exposed male rats s,t 0 CONTROL Rat No. mg% 854 855 856 857 858 859 860 861 80 90 65 95 75 100 100 120 GROUP 1 Rat No. mg% 654 150 655 115 656 120 657 115 658 115 659 125 660 135 661 125 GROUP 2 Rat No. mg% 454 150 455 125 456 75 457 85 458 95 459 90 461 120 462 110 GROUP 3 Rat No. mg % 254 255 256 257 258 259 260 261 100 80 85 75 65 105 90 135 GROUP Rat No. 4 mg% 54 90 55 105 56 105 57 95 58 90 59 90 60 90 61 135 X S.E. 90.62 6.08 112.00 14.85 106.25 8.75 No statistically significant differences were found between any group. 91.87 7.67 100.00 5.51 TABLE 5 Serum bilirubin levels in control and ECD-exposed female rats COM I'ROL Rat No mg? 811 812 813 814 815 816 817 818 0.7 1.4 1.1 0.6 0.6 0.5 0.9 1.2 GROUP 1 Rat No. mg?. 609 610 611 612 613 614 615 616 0.8 1.1 1.0 1.0 0.7 1.1 0.8 1.1 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 1.2 1.0 1.7 1.5 1.4 0.7 0.9 0.8 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 1.4 0.5 1.2 0.6 1.2 1.5 1.0 1.5 GROUP Rat No. 4 mg% 9 1.7 10 0.9 11 1.0 12 1.2 13 1.5 14 1.4 15 1.5 16 1.0 X S.E. 0.875 0.11 0.95 0.057 1.15 0.12 Mo statistically significant differences were found between any group. 1.11 0.13 1.275 0.10 SL 031246 TABLE 8 Serum total protein levels in control and ECD-exposed male rats SL 031247 CON TROL Rat Mo. g% 854 855 856 857 858 859 860 861 6.5 6.5 6.2 6.2 7.5 6.5 6.9 7.5 GROUP 1 Rat No. g% 654 5.8 655 7.5 656 6.3 657 6.3 658 5.9 659 5.9 660 6.3 661 7.0 CROUP 2 Rat Ho. g* 454 455 456 457 458 459 461 462 7.9 6.8 6.5 6.4 7.4 7.0 7.1 6.7 GROUP 3 Rat No. g% 254 255 256 257 258 259 260 261 6.7 7.0 6.7 7.3 6.0 6.0 7.3 7.4 X S.E. 6.72 0.18 6.40 0.21 6.97 0.17 6.80 0.20 No statistically significant differences were found between any group. GROUP 4 Rat No. ' g s 54 6.9 55 6.5 56 7.0 57 7.0 58 7.3 59 7 .0 60 6.3 61 6.9 6.86 6.11 TABLE 6 - Serum bilirubin levels in control and ECD-exposed male rats -- CONTROL Rat No. mg% GROUP 1 Rat No. mg% GROUP 2 Rat No. mg% GROUP 3 Rat No. mql 854 855 856 857 858 859 8 60 861 0.9 1 .2 0.6 0.9 1.6 0.4 0.7 0.8 654 655 656 657 658 659 660 661 0.7 3.1 0.6 0.6 0.8 1.2 1 .0 1 .0 454 455 456 457 458 459 461 462 1.1 1.3 1.7 0.7 1.4 1.4 1.1 1.3 254 1.3 255 1.2 256 0.4 257 0.6 258 0.4 259 0.9 260 1.1 261 1 .2 W X| 0.88 0.82 1.25 0.88 0.13 0.29 0.10 0.13 No statistically significant differences were found between any group. GROUP 4 Rat No. ITLQ % 54 2.0 55 i. i 56 1.2 57 1 . 3 58 0.8 59 1.7 60 0.6 61 1.8 1.31 0.17 TABLE ? Serum total protein levels in control and ECD-esposed female rats SL 031249 CONTROL Rat No. gZ 811 812 813 814 815 816 817 818 7.6 7.0 7.1 6.6 7.0 5.8 8.3 7.0 GROUP 1 Rat No. g% 609 610 611 612 613 614 615 616 7.3 7.2 6.6 6.4 5.5 7.5 7.2 6.8 GROUP 2 Rat No. gZ 409 410 411 412 413 414 415 416 6.7 6.6 7.0 7.7 7.9 7.6 5.6 7.2 GROUP 3 Rat No. g% 209 210 211 212 213 214 215 216 6.2 6.5 7.2 7.9 7.5 8.5 7.5 7.6 X S .E. 7.05 0.25 6.81 0.23 7.03 0.26 No statistically significant differences were found between any group 7.36 0.26 GROUP 4 Rat No. g% 9 8.1 10 6.7 11 7.3 12 7.5 13 7.8 14 7.3 15 8.4 16 7.3 7.55 0.19 TABLE 9 - Serum albumin levels in control and ECD-exposed female rats. SL 031250 C 0 N TRO L Rat No. g% 811 812 813 814 815 816 817 818 3.6 3.3 3.2 2.9 3.5 2.2 3.9 3.2 GROUP 1 Rat No. g% 609 610 611 612 613 614 615 616 3.2 3.3 3.0 2.9 2.0 3.7 3.5 3.7 GROUP 2 Rat No. g% 409 410 411 412 413 414 415 416 3.2 2.7 3.3 3.6 3.8 3.3 2.6 3.4 X S. E. 3.225 0.18 3.16 0.19 3.23 0.14 No statistically differences were found between any group. GROUP 3 Rat No. g% 209 210 211 212 213 214 215 216 2.8 3.1 3.2 3 .7 3.2 3.9 4.0 3.8 GROUP 4 Rat No. gZ 9 4 .1 10 2.7 11 3.7 12 3 .4 13 3.6 14 3.1 15 3.7 16 3.2 3.46 0.16 3.437 0.15 TABLE 10 Serum albumin levels in control and ECD-exposed male rats CONTROL Ra t No. g% 854 855 856 857 858 859 8 60 861 2.7 2.6 2.4 2.7 3.3 2.7 2.8 2.6 GROUP 1 Rat No. g% 654 2.4 655 3.1 656 2.6 657 2.8 658 2.4 659 2.6 660 2.7 661 2.5 GROUP 2 Rat No. q% 4 54 3.1 455 2.4 456 2.3 457 2.6 458 3.0 459 2.8 461 2.4 462 2.8 GROUP '3 Rat No. g% 254 255 256 257 258 259 260 261 2.7 2.7 2.3 2.9 2.3 2.5 2.8 2.8 X S.E. 2.72 2.63 2.67 0.09 0.08 0.10 No statistically significant differences were found between any group. 2.62 0.08 GROUP 4 Rat No. q% 54 2.9 55 2.8 56 3.2 57 2.7 58 2.5 59 2.8 60 2.6 61 2.7 2.77 0.07 TABLE 1 1 Serum GOT levels in control and ECD-exposed female rats. CONTROL Rat No. n'.U/ral 811 812 813 814 815 816 817 818 96 - - 79 64 85 69 GROUP 1 Rat No. mU/irtl 609 610 611 612 613 614 615 616 90 84 78 - 84 85 GROUP 2 Rat No. mU/ml . 409 410 411 412 413 43 4 415 416 88 95 73 71 79 GROUP 3 Rat No. mU/ml 209 210 211 212 213 214 215 216 77 96 83 91 62 78 GROUP 4 Rat No. mU/ml 9 93 10 11 57 12 13 103 14 15 98 16 99 X S.E. 84.20 1.91 81.20 4.54. No statistically significant differences were found between any group ol .17 4.88 91.00 8.55 TABLE 12- Serum GOT levels in control and ECD-exposed male rats CONTROL Rat No. mU/ml 854 855 856 857 858 359 8 60 861 67 - - 95 77 63 GROUP 1 Rat No. mU/ml 654 66 655 - 656 70 657 88 658 88 659 60 660 88 661 85 GROUP Rat No. 2 mU/ml 4 54 455 456 457 458 459 461 462 97 87 93 88 - 90 99 GROUP Rat No. 3 mU/ml 254 255 256 257 258 259 260 261 100 - 63 - 71 95 - X S.E 75.50 6.05 77.85 4.58 92.33 2-01 No statistically significant differences were found between any group. 82.25 9.00 GROUP 4 Rat No. mU/ml 54 63 55 75 56 57 87 58 59 98 60 100 61 63 64.33 5.73 q TABLE 13 - Serum LDH levels in Control and ECD-exposed female rats CONTROL Rat No. mU/ml 611 812 813 814 815 816 817 818 4 80 450 290 320 460 3 70 500 GROUP 1 Rat No. mU/ml 609 610 611 612 613 614 615 616 - 540 550 590 510 520 450 410 GROUP 2 Ra t No. mU/ml 409 410 411 412 413 414 415 416 370 320 390 350 550 490 620 GROUP Rat No. 3 mU/ml 209 210 211 212 213 214 215 216 640 360 640 610 660 380 GROUP 4 Rat No. mU/ml 9 550 10 640 11 510 12 13 550 14 560 15 340 16 360 SL 031254 X S.E. 410.00 31.32 Statistical analysis: Group C vs 3 : P<0.05 510.00 23.20 441.40 42.79 548.30 56.75 501.42 41 .83 TABLE 14 - Serum LDH levels in control and ECD-exposed male rats CONTROL Rat No. mU/ml 854 855 856 857 858 859 860 861 400 510 570 620 340 400 640 GROUP 1 Rat No. mU/ml 654 580 655 540 656 4 60 657 6 30 658 680 659 360 660 660 661 530 GROUP Rat Mo. 2 mU/ml 4 54 455 456 457 458 459 461 462 410 - 630 550 - 510 660 350 GROUP 3 Rat No. mU/ml 254 255 256 257 258 259 260 261 370 550 380 660 590 400 670 550 GROUP Rat No. 4 mU/ml 54 540 55 670 56 57 600 58 6 50 59 610 60 5 30 61 550 X S.E. 497.14 44.86 555.00 37.97 518.33 49.56 521.00 43.34 No statistically significant differences were found between any group 592.80 20.75 TABLE 15 - Serum cholesterol levels in control and ECD-exposed female rats CONTROL Rat No. mg% 811 812 813 814 815 816 817 818 130 110 115 95 100 95 130 110 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 105 110 110 110 80 125 -120 140 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 115 95 115 125 140 105 90 120 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 110 100 115 105 105 115 120 115 GROUP 4 Rat No. mgl 9 1 35 10 95 11 95 12 110 13 120 14 110 15 115 16 no X S.E. 110.62 4.95 112.50 6.12 112.60 5.42 No statistically significant differences were found between any group 110.60 2.40 110.875 4.58 SL 031256 < f *f TABLE 16- Serum cholesterol levels in control and ECD-exposed malerats. CONTROL Rat No. mg% 854 110 855 110 856 115 857 115 858 105 859 95 860 115 861 100 GROUP 1 Ra t No. mg% 654 100 655 155 656 130 657 135 658 150 659 100 660 120 661 155 GROUP 2 Rat No. mg% 454 110 455 130 456 135 457 135 458 150 459 1 35 461 140 462 130 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 115 115 115 125 115 105 120 120 GROUP Rat No. 4 mg% 54 135 55 115 56 105 57 120 58 120 59 115 60 120 61 1 10 X S.E. 108.125 2.66 130 .00 7 .99 133.12 4 .00 116.25 2.06 No statistically significant differences were found between any group. 11 7.50 3.13 SL 031257 TABLE 17 Uric acid serum levels in control and ECD-exposed female rats CON TROL Rat No . mg% 811 812 813 814 815 816 817 818 2.3 2.7 3.3 2.5 2.5 3.5 3.9 4.4 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 2.9 3.2 3.3 3.2 2.6 3.5 3.0 2,6 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 3.6 4.1 2.7 4.1 3.6 2.5 3.6 3.3 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 3.6 2.1 4.5 2.8 3 .4 3.7 3.4 3.1 X S. E. 3.13 0.27 3.03 0.11 3 .43 0.21 No statistically significant differences were found between any group. 3.32 0.25 GROUP 4 Rat No. mg% 9 3.6 10 3.1 11 4.7 12 4.8 13 4.1 14 3.6 15 4 .0 16 2.8 3.84 0.25 SL 031258 TABLE 18-Uric acid serum levels in control and ECD-exposed male rats CONTROL Rat No. mg% 854 855 856 B57 358 859 8 60 861 3.3 3.3 3.0 2.7 3.3 2.5 3.1 3.3 GROUP 1 Rat No. mg% 654 3.3 655 3.6 656 3.9 657 3.7 658 3.4 659 3.5 660 3.9 661 3.8 GROUP Rat No. 2 mg% 454 3.1 455 4 .0 456 3.7 457 3.3 458 3.2 459 3.1 461 3.9 462 3.8 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 3.4 2.9 2.7 3.2 3. 4 3.2 2.9 3.4 GROUP 4 Rat No. mg% 54 3 .7 55 3.5 56 3.3 57 3.2 58 3.9 59 3.8 60 3.4 61 4.4 X S.E. 3.06 0.11 3.63 0.08 3.51 0.13 3.13 0.09 No statistically significant differences were found between any group 3.65 0.13 TABLE 19 - Serum levels of alkaline phosphatase in Control and ECD-exposed female rats SL 031260 CONTROL Rat No. mU/ml 811 812 813 814 815 816 817 818 72 1 36 116 120 104 84 126 62 GROUP 1 Rat No. mU/ml 609 100 610 118 611 70 612 104 613 68 614 96 615 92 616 52 GROUP 2 Rat No. mU/ml 409 410 411 412 413 414 415 416 60 76 80 106 60 114 88 90 GROUP 3 Rat No. mU/ml 209 210 211 212 213 214 215 216 88 84 66 64 112 94 100 122 GROUP 4 Rat No. mU/ml 9 150 10 84 11 102 12 84 13 118 14 108 15 128 16 100 X S.E. 102.50 9.51 87.50 7.79 84.25 6.90 Ko statistically significant differences were found between the groups . 91.25 7.20 96.65 15.73 > SL 031261 TABLE 20 Serum levels of alkaline phosphatase in control and ECD-exposed male rats. CO NTROL Rat No . niU/ml 854 1 76 855 90 856 152 857 68 858 168 859 144 860 861 162 GROUP 1 Rat No. mU/ml 654 58 655 78 656 50 657 50 658 56 659 58 660 72 661 48 GROUP 2 Rat No. mU/ml 454 102 455 80 456 76 457 102 458 102 459 118 461 98 462 122 GROUP 3 Rat No. mU/ml 254 255 256 257 258 259 260 261 194 186 146 154 78 124 108 142 GROUP 4 Rat No. mU/ml 54 94 55 62 56 122 57 1 34 58 134 59 96 60 60 61 116 X S.E. 137.14 14.68 58.75 3.83 100.00 5.66 Statistical analysis : Group 1 vs 4 : P 40.05 <1 1 vs 2 : P <0.05 l II 1 vs C : P <0.001 1 vs 3 : P <0.001 141.5 13.61 102.25 10.44 TABLE 21 Percent concentration of albumin in control and ECD-exposed female rats CONTROL Rat No. mg% 811 812 813 814 815 616 817 818 57.1 51 .0 55.3 50 .0 53.7 50.9 53.3 41.2 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 50.7 50.1 52.9 50.3 52.2 52.3 55.5 47.9 GROUP 2 Rat No. mg% 409 410 411 412 413 414 415 416 50.0 48.8 55.3 50.8 50.8 49.2 47.5 48.0 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 48.5 52.0 52.6 54.8 48.4 57.0 49.3 52.1 GROUP 4 Rat No. mg% 9 49.8 10 51.2 11 47.1 12 48.0 13 53.7 14 43.3 15 50.7 16 49.1 X S.E. 51.56 1.70 51.48 0.80 50.05 0.86 No statistically significant differnecs were found between the groups . 51.83 1 .08 49.11 1.10 SL 031262 TABLE 22 - Percent concentration of albumin in control and ECD-exposed male rats SL 031263 CONTROL Rat No. mg% 854 855 856 857 858 859 860 861 58.8 47.6 45.0 48.9 45.9 55.1 51.3 41.2 GROUP 1 Rat No. mg% 654 655 656 657 658 659 660 661 44.9 43.0 58.3 48.0 48.5 44.4 46.7 44.5 GROUP 2 Rat No. mg% 4 54 455 456 457 458 459 461 462 48.6 41.4 34.1 50.0 41.2 49.2 38.8 41.2 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 45.6 54.4 47.1 47.8 50.2 45.7 48.3 48.3 GROUP 4 Rat No. mg % 54 -47.3 55 44.7 56 45.1 57 54.2 58 54.6 59 40.8 60 47.9 61 40.0 X S.E. 49.26 2.00 47.28 1.71 43.06 2.00 48.42 1.00 No statistically significant differences were found between any group 46.82 5.41 '> CO CO T" 031264 TABLE 23 - Percent concentration of alpha-l-globulins in control and ECD exposed female rats. CONTROL Rat No. mg% 811 812 813 814 815 816 817 818 11.4 1 3 .0 14.6 16.3 14.8 16.9 14.8 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 13.7 14.5 16.4 17.0 14.2 17.7 15.4 16.0 GROUP 2 Rat No. mg% , 409 410 411 412 413 414 415 416 14.9 15.4 14.6 15.9 16.0 19.7 15.0 19.2 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 15.2 18.2 13.3 15.1 13.2 11.6 17.0 15.1 GROUP 4 Rat No. ir.g% 9 13.9 10 16.5 11 16.2 12 12.0 13 14.5 14 16.1 15 12.1 16 18.2 X S.E 15.07 0.81 15.61 0.50 16.33 0.70 No statistically significant differences were found between the groups. 14.80 0.75 14 .93 0.78 table 24 - Percent concentration of alfa- 1 - globulins in control and ECD-exposed male rats SL 031265 CONTROL j Rat No. mg% 854 655 856 857 858 859 8 60 8 61 11.4 16.0 20.0 20.0 16.5 10.9 17.5 20.2 GROUP 1 Rat No. mg% 654 22.5 655 16.0 656 12.1 657 17.6 658 12.5 659 20.3 660 20.4 661 18.1 GROUP 2 Rat No. mg% 4 54 455 456 457 458 459 461 462 16.7 22.7 22.0 14.0 20.6 14.7 21.3 20.7 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 17.8 12.0 17.7 19.3 16.7 17.7 16.5 16.1 GROUP 4 Rat No. mg% 54 14.3 55 15.4 56 18.4 57 13.6 58 16.7 59 19.8 60 16.4 61 19.0 X S.E. 16.56 1.31 16.68 1.62 19.08 1.21 16.725 0.76 No statistically significant differences were found between the groups 16.70 0.79 TABLE 25 - Percent concentration of alpha-2-globulins in 1 and ECD-exposed female rats 9 9 Z t0 CONTROL Rat No. mg% 811 8.2 812 10.2 613 9.4 814 7.9 815 6.4 816 8.5 817 6.5 818 10.3 GROUP 1 Rat No. mg% 609 9.6 610 8.2 611 9 .0 612 10.2 613 10.9 614 8.6 615 6.4 616 8.7 GROUP 2 Rat No. mg% , 409 410 411 412 413 414 415 416 10.7 10.3 7.3 9.6 9.5 9.9 12.5 8.3 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 13.7 7.9 7.6 8.3 13.2 6.7 8.5 12.4 X S.E. 8.42 0.53 8.95 0.48 9.76 0.55 No statistically significant differences were found between any group 9.78 0.99 CROUP 4 Rat No. ir.g% 9 6.9 10 6.5 11 11 .8 12 8.0 13 11.6 14 12.4 15 12.1 16 9.1 9.80 0.87 I\ /.9 ^ 0 TABLE 26 - Percent concentration of alfa-2-globulins in control and ECD-exposed male rats. ------- - - - CONTROL GROUP 1 Rat No. ing% Rat No. mg% GROUP 2 Rat No. mg% GROUP 3 Rat No. mg% GROUP 4 Rat No. mg% 854 855 856 857 858 859 860 861 9.3 8.7 8.4 11.2 9.5 10.9 10.0 10.5 654 8.2 655 13.0 656 9.9 657 8.3 658 10.1 659 9.5 660 10.7 661 8.4 4 54 10.8 455 14.7 456 17.6 4 57 10.0 458 13.3 459 11.5 461 12.5 462 12.7 254 255 256 257 258 259 260 261 7.6 6.6 9.9 10.1 8.4 10.6 9.5 11.5 54 13.2 55 12.4 56 9.9 57 10.8 58 13.1 59 14.5 60 6.6 61 13.7 X S.E. 9.81 0.36 9.76 0.57 12.88 0.85 9.27 0.57 No statistically significant differences were found between the groups 11 .77 0.91 It TABLE 27 - Percent concentration of beta globulins in control and ECD-exposed female rats. CONTROL Rat No. mg% 811 812 813 814 815 816 817 818 14 16.7 14.6 17.6 13.6 16.4 18.2 22.1 GROUP 1 Rat No. mg% 609 610 611 612 613 614 615 616 16.5 19.9 15.4 14.1 16.4 12.8 15.2 18.9 GROUP 2 Rat No. mg% . 409 410 411 412 413 414 415 416 14.9 16.7 15.7 15.9 15.5 16.4 15 .0 16.5 GROUP 3 Rat No. mg% 209 210 211 212 213 214 215 216 15.2 15.8 15.1 13.7 15.4 16.2 15 .5 13.7 X S.E. 15.20 1 .96 16.15 0.83 15.82 0.24 No statistically significant differences were found between any group 15.07 0.32 GROUP 4 Rat No. mg% 9 22.0 10 17.9 11 17.7 12 20.0 13 16.0 14 18.6 15 14.5 16 17.3 18.10 0.87 89ZX TABLE 28 - Percent concentration of beta-globulins in control and ECD-exposed male rats- CONTROL Rat 1-Jo. ng% 854 12.4 855 20,3 cn r1 856 18.4 857 16.9 031269 858 21 .2 00 14.9 860 15.0 861 19.4 GROUP 1 Rat No. mg% 654 16.4 655 21.0 656 12.1 657 17.8 658 18.0 659 17.6 660 14.6 661 19.5 GROUP 2 Rat No. mg% 454 17.9 455 13.4 456 16.5 457 19 .0 458 16.2 459 16.4 461 20.0 462 17.3 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 21.5 20.7 17.7 16.3 18.8 16.5 14.2 17.3 GROUP 4 Rat No. mg i 54 17.6 55 17.0 56 18.4 57 13.6 58 10.8 59 17.2 60 19.6 61 19.0 X S.E. 17.31 1 .07 17.12 0.98 17.08 0.71 17.87 0.84 No statistically significant differences were found between any group 16.65 1 .05 TABLE 29 Percent concentration of gamma-globulins in control and ECD-exposed female rats CONTROL Rat No. mgS GROUP 1 Rat No. mg% GROUP 2 Rat No. mg% GROUP 3 Rat No. mg% 811 9.4 812 9.9 813 6.3 814 6.3 815 10.0 816 9.4 1A 817 5.2 a 05 818 11.8 v-* L 609 610 611 612 613 614 615 616 9.6 7.3 6 .4 8.4 6.6 8.5 7.6 8.7 . 409 410 411 412 413 414 415 416 9.6 9 .0 7.3 8.0 8.3 5.0 10.0 8.3 209 210 211 212 213 214 215 216 IX w 8.53 0.81 7.88 0.39 8.18 0.55 No statistically significant differences were found between any group . 7.5 6.3 11.4 8.3 9.9 8.6 9.9 6.9 8.50 0.60 GROUP 4 Rat No. mg% 9 7.6 10 7.9 11 7.4 12 12.0 13 4.4 14 9.9 15 10.6 16 6.4 8.28 0.87 TABLE 30 - Percent concentration of gamma globulins in control and ECD-exposed male rats. SL 031271 CONTROL Rat No. mg% 854 855 856 857 858 859 860 661 8.3 7.3 8.4 3.2 7.1 8.2 6.3 \ 8.9 GROUP 1 Rat No. mg% 654 8.2 655 7.0 656 7.7 657 8.3 658 10.9 659 8.2 660 7.8 661 9.7 GROUP 2 Rat No. mg% 454 4 55 456 457 458 459 461 462 6 .0 8.0 9.9 7.0 8.9 8.2 7.5 8.1 GROUP 3 Rat No. mg% 254 255 256 257 258 259 260 261 7.5 6.6 7.9 6.7 6.1 9.5 11.8 6.9 X S.E. 7.21 0.64 8.47 0.44 7.95 0.41 7.87 0.67 No statistically significant differences were found between any group GROUP 4 Rat No. mg 5, 54 7.7 55 10.5 56 8.2 57 8.2 58 4.8 59 7.9 60 9.8 61 8.5 8.20 0.59 + TABLE 31 - Na concentration in control and ECD-exposed female rats CONTROL Rat No. mEa/ml 811 812 813 814 815 816 817 818 148 142 150 145 149 150 152 145 GROUP 1 Rat No. mEq/ml 609 610 611 612 613 614 615 616 150 140 147 145 152 147 147 148 GROUP 2 Rat No. mEq/ml . 409 410 411 412 413 414 415 416 150 152 143 150 145 152 141 140 GROUP 3 Rat No. mEq/ml 209 210 211 212 213 214 215 216 150 151 140 147 137 145 148 140 GROUP 4 Rat No. mEq/m 9 143 10 148 11 147 12 143 13 150 14 143 15 1 40 16 140 u 0 X S.E. 147.62 1.18 147.00 1.25 146.62 1.75 No statistically significant differences were found between the groups. 144.75 1.83 144.25 1.31 TABLE 32 Na concentration in control and ECD-exposed male rats SL 031273 CONTROL Ra t No. mEq/ml GROUP 1 Rat No. mEq/ml 854 855 856 857 858 859 860 861 145 152 152 152 145 150 148 145 654 144 655 147 656 147 657 145 658 143 659 143 660 152 661 151 GROUP 2 Rat No. mEq/ml 454 455 456 457 458 459 461 462 147 145 145 143 155 150 148 152 GROUP 3 Rat No. mEq/ml 254 255 256 257 258 ^59 260 261 146 150 150 150 147 145 147 146 GROUP 4 Rat No. mEq/ml 54 55 56 57 58 59 60 61 143 150 140 152 152 147 148 150 X S.E. 148.62 1.16 146.50 1 .22 148.12 1.42 147.60 0.73 No statistically significant differences were found between any group 147.75 1.52 TABLE 33 K concentration in control and ECD-exposed female rats. CONTROL Rat No. mEq/ml 811 812 813 814 815 816 817 818 9.5 8.7 8.5 7.8 8.2 9.5 7.7 9.8 GROUP 1 Rat No. mEq/ml 609 610 611 612 613 614 615 616 9.5 9.9 9.3 9.7 8.7 9.9 9.6 8.2 GROUP 2 Rat No. mEq/ml 409 410 411 412 413 414 415 416 9.6 9.7 9.4 9.1 9.9 8.4 9.8 9.2 GROUP 3 Rat No. mEq/ml 209 210 211 212 213 214 215 216 9.9 7.9 9.9 . 7.6 9.7 9.0 9.8 9.9 GROUP 4 Rat No. mEq/ml 9 9.2 10 9.8 11 9.2 12 9.8 13 9.9 14 9.8 15 9.2 16 9.6 X S.E. 8.35 0.19 9.35 0.21 9.38 0.17 No statistically significant differences were found between any group. 9.21 0.34 9.56 0. 11 SL 031274 TABLE 34 K concentration in control and ECD-exposed male rats. LZT*0 CONTROL Rat No. mEq/ml GROUP 1 Rat No. mEq/ml 854 855 856 857 858 859 860 861 8.2 9.4 8.8 9.5 9.0 8.4 8.5 8.9 654 9 .0 655 9.6 656 9.4 657 9 .0 658 9.8 659 9.2 660 9.8 661 8.9 GROUP 2 Rat No. mEq/ml 4 54 455 456 j r- -t 458 459 461 462 9.9 9.5 9.5 n1 o Vrf 9.8 9.6 8.8 9.9 GROUP 3 Rat No. mEq/ml 254 255 256 257 258 259 260 261 9.6 7.0 7.9 8.4 9.3 9.8 9.6 9.9 GROUP 4 Rat No. mEq/ml 54 9.9 55 9.5 56 9.8 57 9.8 58 9.7 59 9.4 60 7.9 61 9.6 X S.E. 8.83 0.16 9.33 0.13 9.35 0.25 8.93 0.37 No statistically significant differences were found between the groups 9.45 0.23 TABLE 35 ++ Ca concentration in control and ECD-exposcd female rats. CONTROL Rat No. mEq/ml 811 812 813 814 815 816 817 818 10.8 9.4 9.1 9.1 9.3 7.0 12.0 9.1 GROUP 1 Rat No. mEq/ml 609 610 611 612 613 614 615 616 9.7 8.5 9 .0 9 .0 6.7 10.3 9.7 9.3 GROUP 2 Rat No. mEq/ml ' 409 410 411 412 413 414 415 416 9.6 7.7 10.2 8.5 9.0 10.2 8.0 9.9 GROUP 3 Rat No. mEq/ml 209 210 211 212 213 214 215 216 9 .0 9.9 9.5 10.5 9.5 10.8 11.0 9.9 GROUP 4 Rat No. mEq/ml 9 10.5 lO 7.6 11 10.0 12 9.9 13 8.7 14 9.3 15 9.1 16 8.7 X S.E. 9.47 0.51 9.02 0.38 9.13 0.35 10.01 0.24 No statistically significant differences were found between any group. 9.225 0.32 ++ TABLE 36 - Ca concentration in control and ECD-exposed male rats 031277 CONTROL Rat No. mEq/ml GROUP 1 Rat No. mEq/ral 854 855 856 857 858 859 860 861 9.5 9.8 10.4 9.2 9.3 9.3 8.4 9.9 654 8.9 655 6.9 656 8 .0 657 9.8 658 9.3 659 8.3 660 8.8 661 9.6 GROUP 2 Rat No. mEq/ml 454 455 456 457 458 459 461 462 8.8 9.8 8.3 9.9 8.7 8.9 8.8 9.4 GROUP 3 Rat No. mEq/ml 254 255 256 257 258 259 260 261 8.7 9.7 8.2 11.1 8.7 9.3 9.6 10.0 GROUP 4 Rat No. mEq/ml 54 8.5 55 11.1 56 10.3 57 9.3 58 9.0 59 8.9 60 10.2 61 8.6 X S.E. 9.47 0.21 8.57 0.33 9 .07 0.20 9.41 0.32 No statistically significant differences were found between any group 9.48 0.33 TABLE 37 - Cl concentration in control and ECD-exposed female rats SL 03X278 CONTROL Rat No. mEq/ml 811 812 813 814 815 816 817 818 112 97 104 98 93 95 98 101 GROUP 1 Rat No. mEq/ml 609 610 611 612 613 614 615 616 95 105 100 100 108 105 106 107 GROUP 2 Rat No. mEq/ml , 409 410 411 412 413 414 415 416 104 99 100 107 105 118 102 108 GROUP 3 Rat No. mEq/ml 209 210 211 212 213 214 215 216 101 110 107 110 100 102 97 120 GROUP 4 Rat No. mEq/ml 9 102 10 109 11 120 12 97 13 112 14 116 15 103 16 103 X S.E 99.75 2.12 103.25 1.58 105.37 2.12 105.80 2.63 No statistically significant differences were found between any group. 107.75 3.09 SL 031279 TABLE 38 - Cl concentration in control and ECD-exposed male rats. CON TROL Rat No. mEq/ml 854 855 856 857 858 859 860 861 107 119 98 102 118 117 99 114 GROUP 1 Rat No. mEq/ml 654 104 655 98 656 103 657 99 658 102 659 105 660 117 661 110 GROUP 2 Rat No. mEq/ml 454 98 455 98 456 98 457 121 458 107 459 106 461 96 462 100 GROUP 3 Rat No. mEq/ml GROUP 4 Rat No, mEq/ml 254 97 255 94 256 98 257 106 258 100 259 102 260 100 261 109 54 104 55 111 56 108 57 99 58 102 59 100 60 102 61 97 X S.E. 109.25 3.11 104.75 2.18 103.00 2.93 100.75 1 .72 No statistically significant differences were found between any group 102.87 1.65 SL 031280 TABLE 39 - Inorganic P concentration in control and ECD-exposea female rats. CONTROL Rat No. mEq/ml 811 812 813 814 815 816 817 818 7.0 5.8 6.0 4.7 4.9 4.5 5.5 7.2 GROUP 1 Rat No. mEq/ml 609 610 611 612 613 614 615 616 6.4 6.6 4.7 5.6 4.5 5.8 6.0 5.6 GROUP 2 Rat No. mEq/ml 409 410 411 412 413 414 415 416 6.6 5.8 5.5 5.2 7.2 6.2 4.7 5.0 GROUP 3 Rat No. mEq/ml 209 210 211 212 213 214 215 216 6.5 7.5 7.6 3.9 7.8 6.7 6.7 8.0 X S.E. 5.70 0.36 5.65 0.26 5.77 0.30 No statistically significant differences were found between the groups. 6.83 0.46 GROUP 4 Rat No. mEq/ml 9 7.0 10 4.2 11 5 .9 12 7.4 13 7.6 14 8.0 15 6.7 16 5.9 6.59 0.43 TABLE 40 Inorganic P in control and ECD-exposed male rats CONTROL Ra t No. rnEq/ml 854 855 856 857 858 859 860 861 6.2 6.7 5.8 4.3 9.3 4.7 5.0 6.7 GROUP 1 Rat No. mEq/ml 654 655 656 657 658 659 660 661 6.5 10.0 6.4 7.3 6.0 6.3 7.6 6.6 GROUP 2 Rat No. raEq/ml 4 54 455 456 457 458 459 461 4 62 6.9 7.9 7.2 5.0 8.1 6.3 7.0 6.3 GROUP 3 Rat No. mEq/ml GROUP 4 Rat No. mEq/ml 254 255 256 257 258 259 260 261 5.7 6.2 ' 5.8 6.5 4.4 6.2 6.9 7.1 54 9.4 55 7.6 56 5.0 57 6.7 58 7.8 59 8.7 60 6.0 61 7.0 X S.E. 6.08 0.55 7.08 0.46 6.82 0.34 6.10 0.30 No statistically significant differences were found between any group. 7.27 0.50 Ji t* TABLE 41 - Leukocyte numbers in control and ECD-exposed female rats Z 8 ^ C0 CONTROL Rat No. (xIO3) 811 812 813 814 815 816 817 818 10.8 17.0 7.6 6.6 9.6 6.4 1.6 6.4 GROUP 1 Rat No. (xIO3} 609 610 611 612 613 614 615 616 6 .0 8.4 12.8 5.0 12.4 5 .4 10.8 7.6 GROUP 2 Rat No. (xIO3) . 409 410 411 412 413 414 415 416 7.8 9.6 5.4 6.6 8.6 7.6 8.2 7.8 GROUP 3 Rat No. (xIO3) 209 210 211 212 213 214 215 216 8.0 8.0 5.8 10.0 7.8 12.2 8.0 5.4 X S.E. 8.25 1.57 8.55 1.10 7.70 0.45 No statistically significant differences were found between the groups. 8.02 0.78 GROUP 4 Rat No. (xIO3) 9 6 .0 10 8.0 11 4.8 12 7.2 13 5.6 14 6.6 15 5.8 16 6.6 6.325 0.35 SL 031283 TADLE 42 - Leukocyte numbers in control and ECD-exposed male rats. CONT ROL Rat No. (xlOJ) 854 855 856 857 858 859 860 861 6.0 5.6 21 .8 9.6 11 .0 7.4 9 .0 3.8 GROUP 1 . Rat No. (xIO3) 654 655 656 657 658 659 660 661 6.4 11 .0 6.8 5.6 4 .0 4.2 4.6 9.2 GROUP 2 Rat No. (xIO3) 4 54 455 456 457 458 459 461 462 9.8 6.4 6.8 12.0 7.8 5.6 10.8 15.6 GROUP 3 Rat No. (x103) 254 255 256 257 258 259 260 261 9.2 14.0 13.0 7.6 8.4 7.6 6.0 14.6 GROUP 4 Rat No. (xIO3) 54 5.0 55 8.0 56 6.2 57 8.0 58 13.6 59 11.8 60 6.4 61 2.6 X S.E. 9.27 1.97 6.47 0.88 9.35 1.19 10.05 1.17 No statistically significant differences were found between the groups 7.70 1.26 TABLE 4 3 - Hematocrit value in control and ECD-exposed female rats. CONTROL Rat No. % 811 812 813 814 815 816 817 818 40 37 35 43 41 34 38 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 39 31 42 40 44 37 35 39 GROUP 2 Rat No. % 409 410 411 412 413 414 415 416 38 36 30 36 35 37 44 41 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 37 40 33 - 34 47 39 26 X S.E. 32.57 1.78 38.37 1.44 37.125 1.47 No statistically significant differences were found between the groups. 36.57 2.48 GROUP 4 Rat No. % 9 41 10 31 11 43 12 38 13 35 14 28 15 39 16 40 36.87 1.83 SL 031285 TABLE 44 - Hematocrit value in control and ECD-exposed male rats. CONTROL Rat No. % 854 855 856 : 857 858 859 860 861 44 43 39 43 43 46 40 43 GROUP 1 Rat No. % 654 37 655 44 656 37 657 - 658 42 659 34 660 45 661 41 GROUP 2 Rat No. % 454 45 455 45 456 36 457 45 458 39 459 43 461 41 462 45 GROUP 3 Rat No. % 254 255 256 257 258 259 260 261 43 43 48 43 41 41 45 50 GROUP 4 Rat No. % 54 40 55 34 56 40 57 37 58 48 59 40 60 43 61 34 X S.E. 42.62 0.77 40.00 1.54 42.37 1.21 44.25 1.15 No statistically significant differences were found between the groups 40. 33 1 .97 TABLE 45 - Hemoglobin concentration in control and ECD-exposed female rats SL 031286 CONTROL Rat No. % 811 812 813 814 815 816 817 818 20.0 14.0 14.4 15.0 14.8 13.6 8.8 19.0 GROUP 1 Rat No. % 609 1 3.0 610 11 .8 611 12.4 612 15.6 613 13.6 614 11 .6 615 12.2 616 13.4 GROUP 2 Rat No. % 409 410 411 412 413 414 415 416 14.6 18.0 11.8 14.6 12.4 13.2 15.8 13.0 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 12.8 14.6 14.2 14.2 15.6 14.6 13.2 14.2 GROUP 4 Rat No. % 9 13.6 10 13.1 11 13.2 12 14.8 13 6.2 14 15.2 15 15.0 16 15.4 X S.E. 14.95 1.21 11.51 1.51 14.165 0.71 No statistically significant differences were found between the groups 14.17 0.31 13.31 1 .06 TABLE 46 - Hemoglobin concentration in control and ECD-exposed male rats. CO! TROL Rat No. o"b_ 854 855 856 [ 857 858 859 860 861 15.2 15.2 14.2 13.6 15.8 13.2 13.6 15.4 GROUP 1 Rat No. % 654 13.0 655 15.4 656 13.4 657 15.6 658 14.8 659 14.0 660 15.0 661 15.0 GROUP 2 Rat No. % 4 54 455 456 457 458 459 461 462 16.4 14.6 15.0 13.8 15.4 13.2 14.4 15.0 GROUP 3 Rat No. % 254 255 256 257 258 259 260 261 15.4 15.4 13.4 13.4 14.8 13.6 15.0 15.6 GROUP 4 Rat No. % 54 13.0 55 12.2 56 13.4 57 15.0 58 14.8 59 15.2 60 16.0 61 4.0 X S.E. 14.52 0.35 14.52 0.33 14.72 0.34 14.57 0.34 No statistically significant differences were found between any group 12.95 1 .35 TABLE 47 - Erythrocyte numbers in control and ECD-exposed female rats 88?Teo CONTROL Rat No. (xIO6) 811 812 813 814 815 816 817 818 8.2 7.8 7.0 7.3 8.0 3.9 0.8 6.9 GROUP 1 Rat No. (xIO6) 609 610 611 612 613 614 615 616 4.8 8.4 7.4 7.4 8.4 7.0 8.2 4.4 GROUP 2 Rat No. (xIO6) 409 410 411 412 413 414 415 416 6.3 6.0 2.6 7.2 4.8 2.8 7.6 6.9 GROUP 3 Rat No. (x10fa) 209 210 211 212 213 214 215 216 3.4 6.6 7.5 7.2 8.0 8.0 6.4 7.0 GROUP 4 Rat No. (x10b) 9 4.9 10 7.3 11 3.2 12 8.6 13 0.7 14 7. 1 15 8.4 16 8.4 X S.E 6.23 0.91 7.00 0.56 5.52 0.68 No statistically significant differences were found between any group- 6.76 0.52 6.075 1.02 TABLE 48 - Erythrocyte numbers in control and ECD-exposed male rats SL 031289 CON TROL Rat No. (x 10^1 854 855 856 857 858 859 860 861 7.4 6.8 7.0 7.5 9.2 3.5 3.9 7.8 GROUP 1 Rat No. (x10b) 654 655 656 657 658 659 660 661 4.8 7.2 5.7 8.0 7.0 5.8 7.8 8.6 GROUP 2 Rat No. (x10b) 454 455 456 457 458 459 461 462 8.2 8.0 6.4 7.6 8.0 4.2 7.6 8.0 GROUP 3 Rat No. {xio^r GROUP 4 Rat No. (x10b) 254 255 256 257 258 259 260 261 9.0 8.2 7.8 4.0 6.6 5.8 7.6 7.8 54 5.6 55 7.4 56 6.6 57 7.4 58 7.4 59 8.2 60 8.2 61 0.8 X S.E. 6.63 0.69 6.86 0.46 7.25 0.48 7.10 0.56 No statistically significant differences were found between any group 6.45 0.86 TABLE 49 - Mean erythrocyte volume in control and ECD-exposed female rats. SL 031290 CONTROL Rat No. % 811 812 813 814 815 816 817 818 55 50 46 59 55 45 52 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 80 40 57 59 75 50 47 86 GROUP 2 Rat No. % ' 409 410 411 412 413 414 415 416 58 44 99 71 126 60 57 GROUP 3 Rat No. fot 209 210 211 212 213 214 215 216 104 55 42 99 45 67 59 ** GROUP Rat No. 4 % 9 81 10 40 11 131 12 53 13 14 36 15 51 16 53 X S.E 51.71 1.80 61.75 5.91 73.57 10.18 No statistically significant differences were found between the group. 67.28 32.5 63.57 12.48 TABLE 50 - Mean erythrocyte volume in control and ECD-exposed male rats. SL 031291 CONTROL Ra t No. % 854 59 855 71 856 53 ; 857 99 858 59 859 128 860 102 861 58 GROUP Ra t No. 1 Cl 19 654 655 656 657 658 659 660 661 75 59 63 99 57 56 61 55 GROUP Rat No. 2 % 454 455 456 457 458 459 461 462 61 62 48 62 93 100 56 61 GROUP Rat Ho. 3 % 254 255 256 257 258 259 260 261 58 59 65 107 55 69 62 69 X S. E. 78.62 9.74 66.87 5.05 67.87 6.49 68.00 5.85 No statistically significant differences were found between the groups. GROUP 4 Rat No. % 54 69 55 99 56 58 57 50 58 65 59 54 60 58 61 - 64 .71 6 .20 TABLE 51 - Platelet numbers in control and BCD-exposed female iats CONTROL Rat No. {xIO J 811 812 813 814 815 816 817 818 693 280 350 280 371 210 270 579 GROUP 1 Rat No. (xIO3) 609 610 611 612 613 614 615 616 705 527 2 70 573 360 617 317 690 GROUP 2 Rat No. (xIO3) 409 410 411 412 413 414 415 416 330 400 231 250 500 715 412 213 GROUP 3 Rat No. (xIO3) GROUP 4 Rat No. (xIO3) 209 210 211 212 213 214 215 216 300 440 200 350 404 280 280 620 9 231 10 442 11 270 12 580 13 761 14 225 15 305 16 500 X S.E. 380.12 59.44 507.37 60.27 381.00 59.39 No statistically significant differences were found between the groups. 359.00 45.91 414.25 67. 87 031293 TABLE 52 - Platelet numbers in control and ECD-exposed male rats cc c* CONTROL Rat No. (xIO3) GROUP 1 Rat No. (xIO3) GROUP 2 Rat No. (x103) GROUP 3 Rat No. (xIO3) GROUP 4 Rat No. (xIO3) 854 855 856 857 858 859 860 861 441 200 618 698 404 600 271 400 654 720 655 757 656 650 657 877 658 650 659 379 660 397 661 501 4 54 455 456 457 458 459 461 462 612 536 177 680 183 379 312 567 254 255 256 257 258 259 260 261 761 270 740 439 500 637 4 30 320 54 206 55 197 56 306 57 366 58 387 59 540 60 510 61 236 X S.E. 454.00 61.43 616.37 62.46 Statistical analysis : Group 1 vs 2 : P<0.05 " 1 vs 4 : P< 0.005 430.75 69,-07 512.00 65.09 343.5 46.67 V 6Zt0 TABLE 53- Basophilic leukocytes in control and ECD-exposed female rats CONTROL Rat No. o&, 811 812 813 814 815 816 817 818 0 o 0 0 0 0 0 0 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 0 0 0 0 0 0 0 0 GROUP 2 Rat No. % ' 409 410 411 412 413 414 415 416 0 0 0 0 0 0 0 0 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 0 0 0 0 o 0 0 0 X S.E No statistically significant differences were found between any group GROUP - 4 Rat No. % 90 10 0 11 0 12 0 13 0 14 0 15 0 16 0 S 6 Z *0 TABLE 54 - Basophilic leukocytes in control and ECD-exposed male rats CONTROL Rat No. tp 'o 854 855 856 857 858 859 860 861 0 0 0 0 0 0 0 0 GROUP 1 Rat No. % 654 0 655 o 656 0 657 0 658 0 659 0 660 0 661 0 GROUP 2 Rat No. % 4 54 0 455 0 456 0 4 57 0 458 0 459 0 461 0 462 0 GROUP 3 Rat No. % 254 255 256 257 258 259 260 261 0 0 0 0 0 0 0 0 X S.E No statistically significant differences were found between any group GROUP 4 Rat No. % 54 0 55 0 56 0 57 0 58 0 59 0 60 0 61 0 SL 031296 TABLE 55- Eosinophilic'leukocytes in control and ECD-cxposed female rats CONTROL Rat Ho. % 811 812 813 814 815 816 817 818 0 0 o 0 0 0 0 0 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 0 0 o 0 0 1 0 0 GROUP 2 Rat No. % , 409 410 411 412 413 414 415 416 0 0 1 0 1 2 0 0 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 0 1 0 1; 0 0 0 0 -- S.E. No statistically significant differences were found between any group. GROUP Rat No. 4 % 90 10 2 11 0 12 1 13 0 14 1 15 1 16 0 i6 Z te C/S r* TABLE 56 - Eosinophilic leukocytes in control and ECD-exposed male rats. CONTROL Rat No. % 854 855 856 857 858 859 860 861 0 0 0 0 1 0 0 1 X S.E. GROUP 1 Rat No. % 654 0 655 0 656 0 657 2 658 0 659 0 660 0 661 0 GROUP 2 Rat No. % 454 455 456 457 458 459 461 462 0 0 0 1 0 0 0 0 GROUP Rat No. 3 o o 254 255 256 257 258 259 260 261 1 0 0 0 0 0 2 0 GROUP 4 Rat No. % 54 0 55 0 56 0 57 0 58 0 59 1 60 0 61 0 TABLE 57 - Segmented neutrophilic leukocytes in control and bub-exposed female rats. SL 031298 CONTROL Rat No. % 811 20 812 9 813 5 814 9 815 10 816 10 817 11 813 13 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 12 7 8 26 12 19 10 12 GROUP 2 Rat No. % . 409 410 411 412 413 414 415 416 8 7 7 10 20 5 4 9 GROUP 3 Rat No. % 209 7 210 16 211 7 212 23 213 36 214 12 215 12 216 8 GROUP 4 Rat No. % 9 11 lO 17 11 10 12 2 13 15 14 28 15 10 16 16 X S.E. 10.87 1.53 13.37 2.33 8.75 1.75 No statistically significant differences were found between the groups. 15.12 3.54 13.625 2.65 SL 031299 TABLE 58 - Segmented neutrophilic leukocytes in control and ECD-exposed male rats. CONTROL Rat No. % 854 855 856 857 858 859 860 861 17 16 17 31 19 18 20 9 GROUP 1 Rat No. % 654 2 655 2 656 11 657 12 658 11 659 14 660 10 661 2 GROUP 2 Rat No. % 4 54 1 455 9 456 35 457 9 458 1 459 14 461 27 462 19 X S.E. 18.37 2.15 8.00 1.80 Statistical analysis : Group C vs 1 : 14.37 4.24 P <0.005 GROUP 3 Rat No. % 254 255 256 257 258 259 260 261 15 13 18 12 15 19 12 13 GROUP 4 Rat No. % 54 6 55 15 56 7 57 3 58 26 59 20 60 11 61 12 14.62 0.94 12.50 2.70 TABLE 59 - Non segmented neutrophilic leukocytes in control and ECD-exposed female rats oereo CONTROL Rat No. 'o. 811 812 813 814 815 816 817 818 1 0 0 0 0 0 0 0 GROUP 1 Rat No. % 609 0 610 0 611 0 612 0 613 0 614 0 615 0 616 0 GROUP 2 Rat No. % 409 410 411 412 413 414 415 416 0 0 0 0 0 0 0 0 GROUP 3 Rat No. % 209 0 210 0 211 0 212 0 213 0 214 1 215 0 216 0 GROUP 4 Rat No. % 90 10 O 11 o 12 o 13 0 14 o 15 0 16 0 X S.E No statistically significant differences were found between any group TABLE 60 - Non segmented neutrophilic leukocytes in control and ECD- exposed male rats. CONTROL Rat No. % 854 55 856 857 858 859 860 861 0 0 0 0 1 0 0 0 GROUP Rat No. 1 pO. 654 655 656 657 658 659 660 661 0 0 0 0 0 0 0 0 GROUP 2 Rat No. % 4 54 455 456 457 458 459 461 462 0 0 0 0 0 0 0 0 GROUP 3 Rat No. % 254 25 5 256 257 258 259 260 261 0 2 0 1 0 0 0 0 GROUP 4 Rat No. % 54 0 55 O 56 0 57 0 58 1 59 0 60 0 61 0 X S.E. Co TABLE 61 - Lymphocyte percentage in control and ECD-exposed female rats ZT0 CONTROL Rat No. \> 811 812 813 814 815 816 817 818 79 90 95 90 89 90 89 87 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 88 93 92 73 88 80 89 87 GROUP 2 Rat No. % 409 410 411 412 413 414 415 416 92 93 92 90 79 93 96 91 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 93 81 93 76 64 87 88 92 GROUP 4 Rat No. % 9 89 10 81 11 90 12 94 13 85 14 71 15 89 16 84 X S.E. 88.62 1.59 86.25 2.34 90.75 1.79 84.25 3.59 No statistically significart differences were found between any group 85.37 2.50 S t 031303 TABLE 62 - Lymphocyte percentage in control and ECD-exposed male rats. CONTROL Rat No. a'O 854 855 856 857 858 859 860 861 82 84 83 68 79 92 80 90 GROUP 1 Rat No. % 654 98 655 98 656 88 657 86 658 88 659 86 660 90 661 87 GROUP 2 Rat No. % 4 54 455 456 4 57 458 459 461 462 98 91 65 85 99 86 73 81 GROUP 3 Rat No. % 254 255 256 257 258 259 260 261 85 84 80 86 85 81 85 87 GROUP 4 Rat No. ~s----------------- 54 94 55 83 56 93 57 97 58 72 59 89 60 88 61 88 X S.E. 82.25 2.59 90.125 1.77 84.75 4.14 84.125 0.85 No statistically significant differences were found between any group 88 .00 2.75 TABLE 63 - Monocyte percentage in control and ECD-exposed female rats. CONTROL Rat No. O"o. 811 812 813 814 815 816 817 818 0 1 0 1 1 0 1 0 GROUP 1 Rat No. % 609 610 611 612 613 614 615 616 0 0 0 0 0 0 1 7 GROUP 2 Rat No. % 409 410 411 412 413 414 415 416 O 0 0 0 0 0 0 0 GROUP 3 Rat No. % 209 210 211 212 213 214 215 216 0 2 0 0 0 0 0 0 GROUP 4 Rat No. % 90 lO 0 11 0 12 3 13 0 14 0 15 0 16 0 IX w No statistically significant differences were found between any group SL 031305 TABLE 64 - Monocyte percentage in control and ECD-exposed male rats oo TROL Rat No. % 654 855 856 857 858 859 860 861 1 0 0 1 0 0 0 0 GROUP 1 Rat No. % 654 655 656 657 658 659 660 661 0 0 1 0 1 0 0 1 GROUP 2 Rat No. % 454 455 456 457 458 459 461 462 1 0 0 5 0 o. 0 0 GROUP Rat No. 3 % 254 255 256 257 258 259 260 261 0 1 2 1 O O 1 O X GROUP 4 Rat No. % 54 0 55 2 56 0 57 0 58 0 59 0 60 1 61 0 TABLE 65 Urinary ery thro'cyte in control and ECD-exposed female rats. CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 1 3 0 1 0 0 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 0 1 0 1 0 1 0 GROUP 2 Rat No. 409 410 411 412 413 414 415 416 0 0 4 0 0 0 0 0 0 GROUP 3 Rat No. 209 210 211 212 213 214 215 216 0 0 0 0 0 1 0 0 GROUP 4 Rat No. 90 10 0 11 0 12 1 13 0 14 0 15 0 16 0 X S.E. 0.555 0.428 0 0.125 0.125 0 = Absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 031307 TABLE 66 - Urinary erythrocytes in control and ECD-exposed male rats. co CONTROL Rat No. GROUP 1 Rat No. GROUP 2 Rat No. GROUP 3 . Rat No. 854 855 856 857 858 859 860 861 0 0 0 0 0 0 0 0 654 0 655 1 656 0 657 658 0 659 1 660 1 661 0 4 34 1 455 1 456 457 0 458 0 459 0 461 0 462 1 254 255 256 257 258 259 260 261 0 0 0 0 0 0 0 0 GROUP 4 Rat No. 54 55 1 56 0 57 1 58 o 59 0 60 0 61 0 X S.E. 0 0.428 0.428 0 0.285 O = absent ; 1= traces; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. TABLE 67 - Urinary albumin in control and ECD-exposea remale rats. SL 031308 CONTROL Rat No. 811 812 813 814 815 816 817 818 0 2 1 0 0 1 1 0 GROUP 1 Rat No. 609 610 ' 611 612 613 614 615 616 1 1 1 2 1 1 1 GROUP 2 Rat No. 409 410 411 412 413 414 415 416 0 0 1 1 1 0 0 1 GROUP 3 Rat No. 209 210 211 212 213 214 215 216 1 1 1 2 1 O 1 1 GROUP 4 Rat No. 93 10 O 11 1 12 1 13 1 14 1 15 3 16 O X S.E. 0.55 1.14 0.5 1 .00 0 = absent; 1 = traces; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 1.25 V TABLE 68 - Urinary albumin in control and ECD-exposed male rats. SL 031309 CONTROL Rat No. 854 855 856 857 858 859 860 861 1 3 1 1 0 3 2 1 GROUP 1 Rat No. 654 655 656 657 658 659 660 661 1 0 3 0 1 0 1 GROUP 2 Rat No. ' 4 54 455 456 457 458 459 461 462 1 1 - 2 1 2 2 1 GROUP 3 Rat No. 254 1 255 2 256 ' 1 257 1 258 1 259 1 260 3 261 1 GROUP 4 Rat No. 54 . 55 3 56 3 57 3 58 O 59 2 60 2 61 2 X S.E. 1.50 0.87 1.41 1.37 2.14 O = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. TABLE 69 - Urinary sugar in control and ECD-exposed female rats. 031310 CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 1 0 1 1 1 1 0 GROUP 2 Rat No. 409 410 411 412 413 414 415 416 '0 0 0 0 0 0 0 0 GROUP 3 Rat No. 209 . 210 211 212 213 214 215 216 0 1 0 0 0 0 0 0 GROUP 4 Rat No. 90 10 0 11 0 12 0 13 0 14 0 15 0 16 0 X S.E 0 0.571 0 0.125 O = absent; 1 = traces ; 2 = discrete quantities; 3 = high quantity; 4 = very high quantity O * ts t TABLE 70 - Urinary sugar in control and ECD-exposed male rats. CONTROL Rat No. 854 855 656 857 858 859 860 861 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 654 0 655 0 656 0 657 658 0 659 0 660 0 661 0 GROUP' 2 Rat No. 454 455 456 457 458 459 461 462 0 1 0 0 0 1 1 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 0 0 0 - 0 0 0 0 GROUP 4 Rat No. 54 0 55 0 56 0 57 0 58 0 59 0 60 0 61 0 X S.E. 0 0 O. 428 00 0 = absent ; 1 = traces ; 2 = discrete quantities; 3 = high quantity ; 4 = very high quantity TABLE 71 - Urinary ketone bodies .in control and ECD-ex^Oiaed female rats. L 031312 CONTROL Rat Ho. 811 812 813 814 815 816 817 818 1 0 0 0 0 0 0 1 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 0 0 0 1 0 0 0 GROUP 2 Rat No. , 409 410 411 412 413 414 415 416 0 0 0 0 0 0 1 O GROUP 3 Rat No. 209 210 211 212 213 214 215 216 0 0 0 0 0 0 1 0 GROUP 4 Rat No. 90 10 0 11 0 12 o 13 0 14 0 15 o 16 0 X S.E 0-22 0.14 0.25 0.25 0 = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. O TABLE 72 - Urinary ketone bodies in control and ECD-exposed male rats. c m t u it* CONTROL Rat No. 854 855 856 857 858 859 860 861 1 0 1 0 0 0 0 0 GROUP 1 Rat No. 654 1 655 0 656 0 657 658 0 659 0 660 0 661 0 GROUP 2 Rat No. 454 455 456 4 57 458 459 461 462 0 1 0 0 0 0 1 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 1 0 0 0 0 0 0 0 GROUP 4 Rat No. 54 0 55 0 56 0 57 0 58 0 59 0 60 0 61 0 X S.E. 0.25 0.14 0.28 0.25 0 0 = absent; 1 = traces; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. TABLE 73 - Urinary bilirubin in control and ECD-exposed female rats CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 0 0 0 0 0 0 0 0 GROUP 2 Rat No. 409 410 411 412 413 414 415 416 0 0 0 0 0 0 0 0 GROUP 3 Rat No. 209 210 211 212 213 214 215 216 0 0 0 0 0 0 0 0 GROUP 4 Rat No. 90 10 0 11 o 12 0 13 0 14 0 15 0 16 0 X S.E. 0 0 = absent ; 1 = traces 0 00 0 ; 2 = discrete quantities ; 3 = high quantity ; 4 -- very high quantity. ! SL 031315 TABLE 74 - Urinary bilirubin in control and ECD-exposed male rats. CONTROL Rat No. 854 855 856 857 858 859 860 861 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 654 655 656 657 658 659 660 661 0 0 0 - 0 0 0 0 GROUP 2 Rat No. 454 455 456 457 458 459 461 462 0 0 0 0 0 0 0 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 0 0 0 0 0 0 0 0 GROUP 4 Rat No. 54 0 55 0 56 0 57 0 58 0 59 0 60 0 61 0 X S.E 000 00 O = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity; 4 = very high quantity. TABLE 75 - Urinary casts in control and ECD-exposed female rats CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 0 0 0 0 0 0 o 0 GROUP 2 Rat No. . 409 410 411 412 413 414 415 416 0 0 0 0 O 0 0 0 GROUP 3 Rat No. 209 210 211 212 213 214 215 216 0 0 0 0 0 0 0 0 GROUP 4 Rat No. 90 10 0 11 o 12 o 13 0 14 o 15 0 16 o X S.E. 0 0 00 0 = absent ;-1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. o TABLE 76 - Urinary casts in control and ECD-exposed male rats. n cX t CONTROL Rat No. 854 0 855 0 856 0 857 0 858 0 859 0 860 0 861 0 GROUP 1 Rat No. 654 0 655 0 656 0 657 - 658 0 659 0 660 0 661 0 GROUP 2 Rat No. - 454 0 455 0 456 - 457 0 458 0 459 0 461 0 462 0 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 0 0 0 .0 0 0 0 0 GROUP 4 Rat No. 54 0 55 0 56 0 57 0 58 0 59 0 60 0 61 0 X S.E 00 0 00 O = absent; 1 = traces ; 2 = discrete quantities ; 3 = high quantity; 4 = very high quantity. I SL 031318 TABLE 77 - Urinary leukocytes in control and ECD-exposed female rats. CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 1 1 1 1 0 0 GROUP 1 Rat No. 609 1 610 ` 0 611 1 612 1 613 1 614 0 615 1 616 GROUP 2 Rat No. , 409 410 411 412 413 414 415 416 0 0 1 0 0 0 1 o GROUP 3 Rat No. 209 210 211 212 213 214 215 216 1 0 1 1 1 1 0 1 GROUP 4 Rat No. 90 10 0 11 o 12 1 13 0 14 o 15 0 16 1 X S.E. 0.44 0.71 0.25 0.75 0 = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 0.25 TABLE 78 - Urinary leukocytes in control and ECD-exposed male rats SL 031319 CONTROL Rat No. 854 855 856 857 858 859 860 861 0 0 0 0 0 0 1 0 GROUP 1 Rat No. 654 0 655 0 656 0 657 658 0 659 0 660 0 661 1 GROUP 2 Rat No. 4 54 455 4 56 457 458 459 461 462 0 '1 1 0 1 0 0 GROUP 3 Rat No. 254 0 255 1 256 0 257 1 258 0 259 0 260 0 261 0 GROUP 4 Rat No. 54 -- 55 0 56 0 57 0 58 0 59 0 60 0 61 1 X S. E. 0.125 0.14 0.42 0.25 0.125 O = absent ; 1 = traces ; 2 = discrete- quantities ; 3 = high quantity ; 4 = very high quantity. TABLE_ 79 - Urinary crystals in control and ECD-exposed female rats. CONTROL Rat No. 811 812 813 Co 814 815 816 817 818 1 1 1 1 1 1 2 3 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 1 2 2 3 3 1 1 GROUP 2 Rat No. 409 410 411 412 413 414 415 416 1 0 2 2 3 3 2 2 ' GROUP 3 Rat No. 209 210 211 212 213 214 215 216 2 3 1 1 1 1 1 1 GROUP 4 Rat No. 92 10 1 11 3 12 1 13 1 14 2 15 3 16 O < ^ r 0 X S.E. 1.33 2.00 2.00 1.375 O = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 1.625 TABLE80 - Urinary crystals in control and ECD-exposed male rats. SL 031321 CONTROL Rat No. 854 855 856 857 858 859 860 861 2 1 3 1 2 1 1 2 GROUP 1 Rat No. 654 ' 655 656 657 658 659 660 661 1 1 1 1 2 0 3 GROUP 2 Rat No. 4 54 2 455 1 4 56 457 1 458 2 459 2 461 1 462 1 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 1 1 1 3 2 1 2 3 GROUP 4 Rat No. 54 . 55 1 56 1 57 1 58 1 59 2 60 1 61 1 X S.E. ,, 0.625 1.285 1.428 1.75 1.142 0 = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity; 4 = very high quantity. TABLE 81 - Urinary mucus in control and ECD-expose,d female rats. 1322 CONTROL Rat No. 811 812 813 814 815 816 817 818 0 1 1 1 0 1 3 1 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 1 1 1 1 1 1 1 'GROUP 2 Rat No. 409 410 411 412 413 414 415 416 1 0 1 2 1 0 1 0 GROUP 3 Rat No. 209 210 21-1 212 213 214 215 216 1 1 0 1 1 0 1 1 GROUP 4 Rat No. 90 10 O 11 1 12 0 13 o 14 1 15 o 16 1 X S.E. 0.888 1 .00 0.75 0.75 O = absent ; 1 = traces ; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 0.866 )* SL 031323 TABLE 82 - Urinary mucus in control and ECD-exposed male rats. CONTROL' Rat No. 854 855 856 857 858 859 860 861 0 1 0 0 1 1 1 0 GROUP 1 Rat No. 654 655 656 657 658 659 660 661 '0 1 1 0 1 0 1 GROUP 2 Rat No. 4 54 455 456 457 458 459 461 462 1 1 1 3 1 0 1 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 1 0 1 4 1 1 1 1 GROUP 4 Rat No. 54 55 1 56 1 57 1 58 0 59 1 60 1 61 1 X S.E. 0.50 0.571 0.142 1 .25 0.857 O = absent ; 1 = traces; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. TABLE 83 - Urinary epithelial cells in control and ECD-exposed female rats. SL 031324 CONTROL Rat No. 811 812 813 814 815 816 817 818 0 0 0 0 0 0 0 0 GROUP 1 Rat No. 609 1 610 0 611 0 612 s0 613 i 614 0 615 0 616 0 GROUP 2 Rat No. , 409 410 411 412 .413 414 415 416 0 0 0 0 0 0 0 0 GROUP 3 Rat No.- 209 210 211 212 213 214 215 216 0 0 0 0 0 0 0 0 GROUP 4 Rat No. 91 10 O 11 0 12 0 13 0 14 0 15 0 16 1 X S.E. 0 0.25 0.16 00 0 = absent; 1 = traces; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 0.25 0.16 TABLE 84 - Urinary epithelial cells in control and ECD-exposed male rats. CONTROL Rat No. 854 855 856 857 858 859 860 861 0 0 0 0 0 0 0 1 GROUP 1 Rat No. 654 0 655 1 656 0 657 658 0 659 0 660 0 661 0 GROUP 2 Rat No. 454 455 4 56 457 458 459 461 462 0 0 0 0 1 0 1 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 0 0 0 0 0 O 0 0 GROUP 4 Rat No. 54 55 1 56 0 57 0 58 0 59 1 60 o 61 0 X S.E. 0.25 0.14 0.42 O 0.28 0 = absent; 1 = traces ; 2 = discrete quantities; 3 = high quantity; 4 = very high quantity. TABLE " Urinary microorganisms in control and ECD-exposed female rats. SL 031326 CONTROL Rat No. 811 812 813 814 815 816 817 818 3 3 3 3 3 3 3 3 GROUP 1 Rat No. 609 610 611 612 613 614 615 616 1 1 1 1 2 1 3 * GROUP 2 Rat No. 409 410 411 412 413 414 . 415 416 3 2 3 3 1 2 3 3 GROUP 3 Rat No. 209 210 211 212 213 214 215 216 3 1 2 3 3 2 3 3 GROUP 4 Rat No. 92 10 3 11 3 12 3 13 3 14 3 15 3 16 3 X S.E. 3.00 1.428 2.50 2.50 O =? absent ; 1 = traces _; 2 = discrete quantities ; 3 = high quantity ; 4 = very high quantity. 2.87 TABLE 86 - Urinary microorganisms in control and ECD-exposed male rats CONTROL Rat No. 854 855 856 857 ' 858 859 860 861 2 3 3 3 3 3 3 3 GROUP 1 Rat No. 654 655 656 657 658 659 660 ' 661 3 3 3 3 3 2 3 GROUP 2 Rat No. 454 455 456 457 458 459 461 462 3 1 - 1 1 1 1 2 GROUP 3 Rat No. 254 255 256 257 258 259 260 261 3 3 3 3 3 3 3 3 GROUP 4 Rat No. 54 55 3 56 2 57 3 58 3 59 3 60 3 61 3 X S.E. 2.875 2.85 ' 1.428 3.00 - O - absent; 1= traces ; 2 f discrete quantities; 3 = high quantity; 4 ,= very high quantity. 2.85