Document RqBaBOj9Mgp12gKDepMxEOvV

A H A A 6 - O iS G ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1996 Species: Daphnia magna Supplier: In-house cultures, maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: The EC50 values were calculated by the Trimmed Spearman-Karber method. The NOEC was calculated by Steel's Many-One Rank test (Toxstat software, Version 3.1). Test conditions: Dilution water: Dechlorinated City of Duluth, MN, tap water (post carbon filter) that was aerated for 24 hours prior to use. Dilution water chemistry: Hardness: 45 mg/L as CaC03 Alkalinity: 43 mg/L as CaC03 pH: 7.92 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 2000 mg/L primary stock solution was prepared by diluting 2000 mg test substance in 2 L of dilution water. The 002307 remaining exposure concentrations were prepared using a 0.5 serial dilution scheme. Stability of the test chemical solutions: Not noted. Exposure vessels: 250 ml_ borosilicate glass beakers containing 200 ml_ exposure solution. Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.69 - 8.13 (control exposure) 7.78 - 7.94 (1000 mg/L exposure) Temperature range (0-48 hours): 20.1 - 22.4 C Conductivity range (0-48 hours): 152 pmhos/cm (control exposure) 1 4 7 - 1 5 5 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.3 - 8.6 mg/L (control exposure) 7.4 - 8.7 mg/L (1000 mg/L exposure) RESULTS___________________________________________________ Nominal concentrations: Blank control, 62.5,125, 250, 500, and 1000 mg/L. Element values: 48-hour EC50 = 479 (378 - 607) mg/L 48-hour NOEC = 250 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 48-hour EC50 was determined to be 479 mg/L with a 95% confidence interval of 378 to 607 mg/L. The 48-hour No Observed Effect Concentration (NOEC) was determined to be 250 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________ _____________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study 002308 lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES____________________________________________________ This study was conducted by AScI Corporation, AScl-Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number P 2581,1996. OTHER___________________________________________________________ Last changed: 6/26/00 002309 UK?COPY AVAILABLE AScI CoriMiMlioti'ASvl Duluth environmental Tenting Division Submitted lo .1M Co. - I ASvi SluUy ID# 5010 0(,S STUDY TUFF. ACUTE TOXICITY OF FC-'.>03EF. FIGH I' WATER(TM) ATC 3%. AR-AFFF ID PA PUMA AJACXA DA TA STANDARD OECD GUIDELINE 202 s t u d y c o m im .e t e d November 7. 19% TESTING FACILITY AScI Corporation/ASel-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (2 IS) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS :i r 5' ; i BEST COPY a v a il a b l e AScI Ct'rpruijui''ASc1 Duluih Cuvtruiiincnlai Testing Diwmom Submitted to 3M Co 11/9b ASel Study IDa 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-603EF Light Water(TM) A'l'C 3c{ , AR-AFFF Duphnia iihi^mi. OECD Guideline 202. 3M Environmental Technology and Services, Building 2-312-09. 935 Bush Avenue, St. Paul. MN 55133. Susan A. Beach. 3M En\ironmental Technology and Services. Building 2-3E-09. s>35 Bush Asenue. St. Paul, MN 55133. Tel No. (012) 778-7452. { P25S1. AScI Corporation AScI-Duluth Environmental Testing Disision. 4444 Airpark Boulevard. Duluth, MN 55811; Tel. No. (218) 722-4040. Joe Dierkes. Joe Amato. Donald Mount. October 29-31. 199b. Alan Mozol. Report Signature' Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScl-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. &Hi*>AVf;'t-4Y' ' 4K-?f <n * V, , t ^ R -AH' . '> *' X . JW H. -S p o n s o r: 3M Company -, ' Sponsor Study ID# P4581 ''. ,>T. ;, . r-hi .>-UaVv Vv`..-ui-*4v^'aar<.- * . \4~ W ;:' BEST COPY AVAILABLE ASvl C\r|H>rjlnMi''AScl-Duluili t i n iriTimcnlul To-lti*: D ivmuii Submitted III 3.M Cu - I l -9() A S d Study ID# 5010 0(>S 2.0 OBJECTIVE(S) To determine the 48-h ECSu and 48-h NOEC (no observed effect concentration) of the test substance, FC-603EF, for Daphnia magna. This study was conducted according to the ASel study plan retained by the Sponsor and OECD Guideline 202 (OECD 1993). Test Substance Test Organisms Dilution Water Exposure Chambers 3.0 TEST METHODS Properties: FC603EF. amber-colored liquid. s|>ccific gravity ca. 1.06 (water = 1), vapor pressure ca. 15.S mmHg. stable, and completely soluble in water. Test concentrations: 0 (dilution water control). 62.5. 125, 250. 500. and 1000 ing/L. High test concentration solution prepared by diluting 2000 mg of test substance in 2 L of dilution water. Lesser concentrations were prepared using a 0.5 serial dilution scheme. Source: Healthy, less than 24-h old Daplwia magna neonates obtained from AScl stock cultures maintained in water of test dilution water quality. Use in rest: Gravid adults isolated in dilution water 24 It before the test initiation. Used 5 organ isms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 45 mg/L (as CaCOj) and a pH of 7.92. Aerated for 24 h prior to using in the test. .- Type: 250-ml borosilicate glass beakers. Use in test: 200 nil of dilution water or test solution/chamber. During the test,2kept covered, except when experimental observations were made. b e s t c o p y a v a il a b l e ASwl AScI Dulutl* l ; n \ i r - 'fitstv'i.l.il t ' c v h i i ^ D i \ iM t> n SiiliimiU'J lu 3M C o - 1 1 A S J Stovtv ID* 5 0 t 0 063 Incubation Observations Endpoint Calculations Duraion: 48 h. Daily pftoioju'riod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Ti'nipi'ninoi-: 21'C . Bioioi'ical: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure clumbers were gently agitated). Did not remove anv daphnids from the exposure chambers at 24 h. Wawr cht'iniMi'. : (1) At lest initiation- total hardness and alkalinity. (2) daily- temperature. pH. DO, and conductivity. All determinations were made according to APHA methods (1995). EC,,: Trimmed Spearman-Karber method. NOEC: Steel's Many-One Rank Test (TOXSTAT statistical software, Version 3.1, University of Wyoming). ffi*"'r,L'Sy,* Sjx>n*or: 3M Company . Sponsor Study ID# P2581 5* *f f i ' V4V.'i.t'M-'.' S && r-. >-'*-'>v.'iv4*^ ,"-"-v,i-'Mi xvji-..>&*:. . , -V;-'-/*<. *' -*V-.. '4ti- vA *v--& . ,u . ;W v; I I ?f, ', * ' tirj v"^r'' ' ',* <-: - ^ - ~` f* * ' **I& r BEST c o p y a v a il a b l e AS*. I C*f|Mralin`ASv-l -Dululh Ln.iriuuncal.il Tcsuiij: Division SulMIHllCil lo 3M Co - 1I Oft AS* I ShaU ID* 5010 068 3. RESULTS Test Substance Toxicity (Pertinent data are in Table 1) Ranges of Water Chemistry Parameters 24-h 12C\,,: > 1000 ing/L (Not Calculable). 4S-h LC\,: 479 mg/L (378-607). 4S-h XUICC: 250 mg/L. Tom! hardness ms CaCOs ing/Lt: 45. Alkalinity uis CnCO, my 'L)\ 43-51. Temperature t Ci: 20.1-22.4 C. Range exceeded bv 4-0.4 "C. Exceedance likelx had no bearing on toxicity measurement. DO mipd.,: 7 4-9.0. 7.69-8.13. Specific conductivity tinhos/cnir. 132-19!. 4.0 TEST Q.VQC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity test (this test is optional). ' Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 83% of the air saturation value at 21.0C. The test duration was 48 h. Current control chart for D. magna acute testing is included in the raw data package. Sponsor: 3M Company Sjwnsor Study ID# P2581 ' 'i ~j - / ,'S '$VV` 0 f X ' !?Tx` S '' V *^ .`fe'J.-f ' U U i.O I 'i ASel O irpnrition- AScI-Duluth L m ir.-nm cnlal Testini! D iwsmii SuhuiHlcd t*> 3M Co - 11 K)b ASel Study ID# 5010 068 5.0 REFERENCES American Public Health Association (APHA). 1095. Standard Methods l'or the Examination o Water and Wastewater. APHA, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassavs. Environ. Sci. Technol. 11(7):714-719; Correction 12(4):417 (1978). " '- Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington. D.C. University of Wyoming. 1989. Toxstat Version 3.1. Fish Physiology and Toxicology Laboratory, Department of Zoology and Physiology. University of Wxommg, Laramie. WY. S|*m*or: 3M Company Sjwnsor Study iDU P2581 11 6 AScI Corporation/ASel-Duluih Environmental Testini; Division Submitted to 3M Co. - 11/96 ASel Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF FC-603EF LIGHT WATER(TM) ATC 3% AR-AFFF TO D. MAGNA Test Substance C o n cen tratio n (m g/L) 0 62.5 125 250 500 1000 C um ulative N um ber and Percent o f Dead and Im m obilized Organisms* 24 h 4S h 0 .(0 ) 0 (0 ) 0 (0 ) 0 (0 ) 3 (15) 5 (25) 0 (0) 0 (0 ) 0 (0 ) 0 (0) 12 (6 0 ) 17 (S5) * Percentages are in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS ECm and NOEC 24-h EC 48-h E C 48-h NOEC D ata (m g/L) >1000 479 250 95% C onfidence Interval N ot Calculable 378-607 N ot A pplicable \ www* BEST COPY AVAILABLE Sponsor:. 3M Company Sponsor Study ID# P258I 3 : W M : >' '\v ;?h`fS/? x?.i/fs W ;\ ` '*S'` 002316