Document RpxjaEwxbrz72bdwQeLRDe02B
ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUBSTANCE
(DAPHNIA MAGNA)
Identity:A mixturecontainingperfluorooctanesulfonatmea;y also be referredto as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfoniacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 101. The testsample isFC206CF. Currentinformationindicatesitisa mixtureof0.5% PFOS, 10% diethyleneglycolmonobutyl ether,78.08% water,1.375% acrylicfoamer, 1.5% sodium octylsulfate1,% sulfonatedalkylethersalts,6.0% urea,0.5% triethanolaminea,nd 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: OECD Guideline202 Type: Acute static GLP: No
Year completed: 1991
Species: Daphnia magna
Supplier: In-houseculturesmaintainedatEnviroSystemsDivisionR,esource Analysts,Inc.,Hampton, NH.
Analyticalmonitoring: Temperature,pH, conductivitya,nd dissolved oxygen.
Exposure period: 48-hours
Test organism age: <24 hours old
Statisticalmethods: No calculationpserformed, based on study results. Test conditions:
Dilutionwater: Filterednaturalwellwater Dilutionwater chemistry:
Conductivity: 840 pmhos/cm
pH:
7.6
Lighting: Cool-whitefluorescentlightsat20 pEin/seC/M2. A daily photoperiodof 16 hours lightand 8 hours dark was maintainedthroughoutthe testingperiod.
Stock and testsolution preparation: A primary 1000 mg/L stocksolution was prepared and added directltyo createtestsolutions. Exposure vessels: 250 mL beakers containing200 mL exposure solutionat a water depth ofapproximately6 cm. Number of replicates:4 Number of daphnids per replicate:5
Number ofconcentrationsf:iveplusa blankcontrol Water chemistryduringthestudy:
pH range(0-48hours): 7.6- 8.3(contreoxlposure) 7.7- 8.3(1000 mg/L exposure)
Temperature range (0-48hours): 19.0- 20.2 'C (controelxposure) 19.1- 20.3-C (1000 mg/L exposure)
Conductivityrange (0-48hours): 840 - 880 pmhos/cm (controelxposure) 850 - 880 pmhos/cm (1000 mg/L exposure)
Dissolved oxygen range (0-48hours): 7.6- 8.3 mg/L (controelxposure) 7.7 - 8.3 mg/L (1000 mg/L exposure)
RESULTS
Nominal concentrations:Blank control1,50,250,400, 600, and 1000 mg/L.
Element values: 48-hourEC50 = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 48-hour EC50 was determined to be >1000 mg/L. No mortalitoyr sublethaleffectswere observed.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 2.This study meets the criterifaorquality testing.However, the sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportion inthe solution.
REFERENCES
This testwas conducted by EnviroSystems DivisionR,esource Analysts, Incorporatedo,f Hampton, NH atthe requestofthe 3M Company, St.Paul, MN, Lab Request number J1884-4,1991.
OTHER Last changed: 6/28/00.
Study Title Static Acute Toxicity of rC-206cr
to the Daphnid, Dapbais magns
Authors Timothy J. Ward Robert L. Boori
Study Comploted June, 1991
P*rformi@a 16aborit@@ --ZnviroSystess Divisi Resource Inalysts,Incori*rated
One Lafayette Road-'@' Hampton, Now Hampshire- 03842
Fag* :L of 14
1. SURMT
The &cut* toxicity of FC-206CY to the daphnid, Daphnis magna, is described in this final report. The test was conducted for 3H Company for 48 hours during June 10 to 12, 1991, at the EaviroSystess Division of Resource Analysts, Inc. in Hampton, New Hampshire. it was conducted by Jeanne Nagazu, Peter Kowalski, Zllen Stanford, Robert Doeri, and Timothy Ward.
The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 t IOC. The dilution water was filtered natural well water collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC-206CF were: 0 mg/L (control),150 mg/L, 250 mg/L, 400 mg/L, 600 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations.
Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control dapbnids had an average wet weight (blotted dry) of 0.0006 g, resultingin a loading rate of 0.015 g/L. All daphnids were in good condition at the beginning of the study.
The test substance was not toxic to daphnids. Exposure of dapbaids to the test substance resulted in a 48 hour IC50 greater than 1,000 mg/L rC-206CF.
page. 2 of -14
II. TABLB Or CONTEM
SECTION:
PAGI
1.
Summary
2
ii. Table of contents
3
III. Index of Tables
4
IV. Introduction
5
V.
Methods and Materials
5
Vi. Results
8
VIZ. References
Appendix A. Water Quaiii bita from Toxicity Test
12
"t 7:t
Page 3
ixr.nmimorTuLzs nD rrcnzs
Table 1.
Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test
Table 2. Survival data from toxicity test
Table 3.
Median effective concentrations (EcSos) from toxicity test
Table A.l. Conductivity, pff,temperature, and dissolved oxygen concentration measured during toxicity test
PAGE 6
9 10 13
Page. of 14
KIIFORE
nmommon
This study was sponsored by 3H Company, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC-206Cr to the dapbnid, a freshwater invertebrate. The report contains sections that describe the methods and materials esploy*d in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test.
TEST SUBSTANCE:
V. METHODS AND MATERIALS
FC-206er (EnviroSystess Sample Number 29382) was delivered to EnviroSystess on Kay 23, 1991. It was contained in a 4 oz. Plastic bottle that was labelled with the following Information:IJ1884-4, FC206CF". rC-206CF (a light brown liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately1.0 grans) will be retained at EnviroSystess for a minimum of 10 years.
DILUTION WATER:
Water used for acclimationof test organismsand for all toxicity testing was collected from wells at EaviroSystem in Hampton, New
Hampshire. , Water was stored in 500-gallon polyethylene tanks where it was
aerated'. Results of chemical analysis of a representative sample of water are.presented in Tible@l.
TEST ORGMSN:
-Juvenile daphaids employed as test organisms were from a single
source -'andL@w@ere .identified using an appropriate taxonomic key. Daphnids
'used'@ tliei-e7st'iere' produced from an in-houie culture and were ess than
24-;
tha' start of the test'."Control dapbuids were weighed at@z'
the toxicity test. -,Priorto testing, daphnids were
iaintained-..:.1i0n0.% dilution.water under.static conditions in 4 liter glass
D@iini'.,,.,.icclizatdiaopnhaids were @not:,treated for disease and they. of apparent,@sickness, injarlei,' and abnormalities'a b
begin'n g'::'.-"of7"t@els?t.b,e During the.acclimation period 24 hours"prior to'---@,
the-,.t-e,st'--i@nitiation the temperature was 19.5*C. Daphnids were fed yeast
trout"
and/or the freshwater alga'.S-o@lonastrus capricoroutuwonce
or twice'dailybefore the test.
k'@
NOU=Ar&^W-&bd*qd Olt
Table 1.
Chemical characteriz@lion of
representative sample of
natural well water used as'dilutionwater for toxicity test
Parameter
PH
Conductivity
Organochlorine pesticides
Organophosphorus pesticides
Polychlorinated biphenyis
Unit of Measurement
PH units
unhos/cm
ug/L
Reporting Limit
0.5
ug/L
0.5
ug/L
0.5
Measured Value 7.6 840 I(D
ND
ND
Notes: I. None detected at or above the reportino limit.
2. Ph and conductivity were measured in dilution water
collected from a control test,vesselprior to
introduction of collected
organizas'arid,-Pesticidaend
PCB
d ata is
during.routine biannual.water quality testing.
7,@ 7
P e 6 of 14
TOXICITY TESTING:
The definitivetoxicity test was performed during June 10 to 12, 1991. It is based on procedures of the OECD (1984). The test was conducted at a target temperatureof 20 t 10C with five concentrations of test substance and a dilution water control. A 1,000 mg/L stock solution was formulated without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control),150 mg/L, 250 mg/L, 400 mg/L, 600 mg/L, and 1,000 mtr/L.
Twenty daphuids were randomly and equally distributed among four
replicates of each treatment. The test was performed in 250 al glass
beakers that contained 200 ol of test solution (water depth was approximately 6 ca). Test vessels were randomly arranged in an
incubator during the 48 hour test (a random numbers table was used to
select the location of each vessel). A 16 bour light and 8 hour dark
photoperiod was automatically maintained with cool-white fluorescent
lights that provided a light intensity of 20 UES-la-2.
Aeration
was not required to maintain dissolved oxygen concentrationsabove
acceptable levels. Dapbnids were not fed during the test.
The number of surviving organisms and the occurrence of imobilization (inabilityto swim within 15 seconds after gentle
agitation) or other sublethal effects (lossof equilibrium,erratic swimming, loss of reflex, excitability, discoloration,or change in behavior) were determinedvisually and recorded initiallyand after 24 and 48 hours. Dissolved oxygen (YSI Model 57 meter; instrument number PRL-3), pE (Beckman model pHl 12 meter; instrument number PRL-51),
conductivity (Cole Parser model number 1491-60, instrument number PRL-50), and temperature (ASTM mercury thermometer; thermometer number 2265) were measured and recorded daily in each test chamber that contained live animals.
STATISTICAL EMODS:
Results of. the toxicity test could not be interpreted by standard statistical techniques (Stephan, 198.3)because 50% survival or greater occurred in all tested concentrations.
7 of 14
R
ArooP^kV@Sut)WdkdNMyTLFM
vr. RESULTS
All tsst vessels containing FC-206CP were initially clear and remained clear throughout the test. Biological and water quality data
generated by the acute toxicity test are presentedin Table 2 and
Appendix A, respectively. One hundred percent survival occurred in the
control exposure.
dry)
Control dapbnids bad an average wet weight (blotted
of 0.0006 g at the end of the test. Loading rate during the
toxicity test was approximately 0.015 g/L.
The 24 and 48 hour EC50s for daphnids exposed to FC-206CF are presented in Table 3. The 48 hour ICSO was greater than 1,000 mg/L FC-206CF.
Page 3 of 14'
PosaumAnotvfWt- SbddbV d
Table 2. Survival data from toxicitytest
Nominal Concentration
(mg/L)
rep.
0 control 1 2 3 4
150
1
2
3
4
250
1
2
3
4
400
1
2
3
4
600
1
2
3
4
1 000 2
Number Alive 0 hr 24 hr 48 hr
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5,
5
5
5
5
5.
5
5
5
Number Affected
Ohr
24hr 49hr
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
o
0
0
0
0
0
0
0
0
0
0
0
0
0
0
o
0
0
0
0
0
o
0
4t
Table 3. Nsdi&u effOctivO concentrations (ICCSO,)from toxicity test
Exposure period
95 percent
EC50
confidence limits
Calculation method
24 hours )1,000 mg/L 48 hours )1,000 mg/L
'V@7 )f 14
vir.zznmcics
OECD. 1981. Decision of the Council Concerning the Mutual Acceptance of
Data in the Assessment of Chemicals. Aznex 2. OECD Principles of Good LaboratoryPractice. C (81) 30 (Final).
030.
1984. Guidelines for Testing of Chemicals. Section 2: Effects On
Biotic Systeas. Method 202, Dapbuid sp., Acute Iamobilization Test and Reproduction Test. Adopted April 4, 1984.
-tIk
@4- -1"
d
AppendiX A. WATER OUALITY DATT FRom ToxrciTy TEST
tie r
12,nr -IL4
Table A.I. Conductivity, pn, tempo-sture, And dissolved oxygen concentration measured during toxicity test
Nominal concentration
(no/L)
Rep.
0 control 1 2 3
4
150
1
2
3
4
250
1
2 3
4
400
1
2
3 4
600
1
2
3
4,
11000
1-
2
3
4
Conductivity (usho/cm)
0
24
48
hr
hr
hr
870
ego
$40
870
880
840
870
880
840
870
880
840
860
900
840
860
900
840
860
900
840
860
900
840
860
880
$40
860
880
840
860
880
840
860
880
940
860
870
$40
860
870
840
860
870
840
860
870
850
860
870
850
860
870
850
860
870
850
860
870
850
860'
8801@,
$50
860
880
$50
-860
-880,-
860. 880
0
hr
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.7 7.7 7.7 7.7
PH
24
hr
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7,
7.7 7.7
7.7
48
hr
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 8.3 8.3 @S. 3
83
.8.3 8.3
.3 of@ld,
Table A.I. Continued.
nominal
concentration
(mg/L)
Rep.
0 control 1 2 3 4
150
1
2
3
4
250
1
2
3
4
400
1
2 3-
4
600
1
3
Temperature (OC)
0
24
48
hr
hr
hr
20.0 20.2 20.1 20.2
20.2 20.2 20.1 20.2
20.3 20.1 20.2 20.1
20.1 20.2 20.2 20.3
20.0 20.1 20.1 20 .2
2
19.0 19.0 19.1 19.1
@19.1 19.3 19.4 19.3
19.1 19.0 19.2 19.1
19.4 19.3 19.2 19.3
19.1 19.2 19.0 19.0
19.3 19.3 19.3 19.3
19.0 19.1
19.2 19.1
19.3, 19.4.
19.4
19.5
19
19.3,: 19.4
19.2
19 2,,-'i@'.19
19.1'
19-'319.'3i-
Dissolved Oxygen (mg/L)
0
24
48
br
hr
hr
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7.6 7.6 7.6 7.6
7 .7
77 7.7.
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 7.7 7.7 7.7
7.7 77 7.7 7.7
7.7 7.7 7 7. 7:7
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 8.3 8.3 8.3
8.3 .3
@3 S.@ 3
8 31,?,;,
, .1@ps -14@ li7