Document Rpn02aa0Mm4wmkX0wMKyj22Dz
Lo
AR226 _ 0775
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity:
A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203S. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M product lot number was 3086. The test sample is FC203S (F2132-1). Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 2.66% Sultone foamer, 3% sodium octyl sulfate, 0.10% sodium lauryl sulfate, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely
characterized concentrationsof impurities. Data may nota.ccurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD:
Method: U.S. EPA
Type: Acute flow-through
GLP: No
Year completed: 1988
Species: Salmo gairdneri
Supplier: Mt. Lassen Trout Farm, Red Bluff, California.
Analytical monitoring: Temperature, pH, and DO
Exposure period: 96-hours
Statistical methods: LCs values calculated using the Stephanetal.
computer program, 1978.
Test fish age: Juveniles
Length and weight: Average length = 39 mm
Average weight = 0.86 g (wet)
Loading: 0.57 g fish/L
Pretreatment: Unknown
Test conditions:
Dilution water: Blended water which is a mixture of reverse osmosis
and ABC well water.
Dilution water chemistry:
Hardness: 40 - 48 mg/L as CaCOs
Alkalinity:
44 - 56 mg/L as CaCO3
Lighting: A daily photoperiod of 16 hours light and 8 hours dark was
`maintained throughout the testing period.
`Stock and test solution preparation: The primary stock solution of 160,000 mg/L was prepared by dissolving 160 goftest material in 1 L of deionized water. Test solutions were intermittently introduced to the test vessels via a
001393
proportional diluter system with a flow rate of 1 L of test solution at an average rateof 8 times per hour. Flow-through rate: Approximately 6.4 times per day. Stabilityofthe test chemical solutions: Not noted Exposure vessels: Aquaria containing 30 L of test solution Number of replicates: 1 Numobfefirsh per replicate: 20 Number of concentrations: five plus a blank control Water chemistry during the study:
pH range (0-96 hours): 7.4 -7.8 (control exposure) 7.4 - 7.8 (400 mg/L exposure)
Temperature range (0-96 hours): 13-13 C (control exposure) 12-13 C (400 mg/L exposure)
Dissolved oxygen range (0-96 hours): 7.7- 8.9 mglL (control exposure) 7.2-9.0 mgL (400 mg/L exposure)
RESULTS
Nominal concentrations: Bk control, 25, 50, 100, 200, and 400 mg/L
Element values: 96-hour LCso = >400 mg/L 96-hour NOEC = 400 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
The testing laboratory greater than 400 mg/L
was due
unable to conduct the to insufficient sample
study size.
at
concentrations
CONCLUSIONS
The test mglL.
sample
96-hour
LCso
for
rainbow
trout
was
determined
to
be
>400
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY HReolwiaebvileirty,: saKmipimliescphurriatynkwiansg 2n.otTpersotpienrglymecehtarsatchteercirzietedriaanfdortqhueaslittuydytesltaicnkgs,
001394
analytical confirmationof the amountoffluorochemical proportion in the solution. The first five pages of the final report are missing. REFERENCES Test was conducted by ABC Labs, of Columbia, MO at the request of the 3M Company, 1988. OTHER Last changed: 6/28/00
001395
"Internal Correspondence/3M
EAI $9022
ce: HK.l TW.. PLiikveerm-or2e36--3A2-3061-2a-01 MD.. TB.l NEelunmaabnara-wy23-6-22A1--0214-05
To:
D. R. RICKER - 236-18-10
FROM: EB. A. REINER - ENVIRONMENTAL LAB/EE & PC - 21-20-05
SUBJECT:A
----
DATE: DECEMBER 28, 1988
-
oTInhneFrheCis-gp2ho0en3ss5te Ltcotooncy3oe0u8n6rt.rarteiqTuohenestrt,eesstuaeldtssalwamosofn4t0hf0irsymgbb/iilooaassassndaayytwahareserecawtoetnradecuhcetndeo.d atdhivsersweaseftfheecthsighaetstthicsoncceonntcreanttiroatniotnh.at Acocuclodrdibneg tteosteAdBCwiLtahbs,the amount of sample provided. Hhaovpeefualnlyy,quetshtesieonsreosrultcsoncaerrensa,deqpulaetaese tofeemleetfryeeourtoneceodnst.act Ifme yooun 778-5079.
EAR/mb Attachment: Bioassay Results on FC-203S
001396,
suoARy
troutA (9S6a-lhmoourgaatcrudtneerfil)ow-wtahsroucgohndutcotxeidcitfyorstu3dXy CoofmApRanGyMOEfsrtomo Orcationbbeorw c14o,ntr1o98l8wetroeOcutsoebderin18a, n1o9m8t3n.al Tdwoesnitnyg friesghimpeerofte2s5t, c5o0n,ce1n0c0r,ati20o0n, plaunsd t40e0mpemrga/tlu.re Wwaetreer ccohnesmiisstternyt tmheraosuugrheomuetntstheoftedirssooflvetdhe oexxypgeerni,mepnit aanndd were within the protocol specifications.
Fron the data collected during
confidence limits was calculated to
this study, a
be 600 mg/l
96-hour LC, with 95%
based on fhe nominal
cefofneccetnstraotcicounrsreodf aFtC-2a0n3y. exNpeoistuhreer cmoonrcteanltirtaytionnor orsubcloentthraoll/.behaAvsiordael-
tteorxniicnietdy tboy rtahiinsbowstturdoyu,t awas964-0h0ourmg/mlo-beafsfeedctoncoancleanctkraotfiomnorotfaliFtCy-20o3r
abaormal responses.
A #372426 001397
INTRODUCTION
Blo-C3he4ntCaomtpraynyLacboonrtartaocrtieeds,thIencA.q,uattoiccoTnodxuicctoloagyfiDvievicsoinocnenotfraAtniaolnytipclauls ctoontrraoilnbofwlotwr-otuhtrou(Sgahlmtooxgiaciirtdynetreis)t. toThedetperrimmianrey otbhjeecttoixvieciotfy tohfisFCs-t2u0d3y wtaesrnitnoinegstitmhaetemetdhieanaculteethatloxiccointcyentofratFiCo-n203(LCt,o Faimibfowpotsrsoiubtleb,y adnedr Tehxiasminviansgacscuobmlpeltihsahle/dbebhyaveixoproaslinrges2p0onsreastnbdouvritnrgoufthepecroucrosneceonftrtahteiontesatn.d con96trohlouranedxpmoosnuirteordiunrgatsiuornv.ival and sublethal/behavioral responses over
C-203ThecatuesrimngLSCO,Y, maosrtausleidty inafttehrisa resppoercti,fiedds etxhpatosucroencpeenrtiroadt.ion Thoef AsBtCudyPrvoatsococlondNuucatbeedr f8r0o0m7 O(cAtPoPbEeNrDIX1)4, as198a8pprtooveOcdtobbyerMo1h8a,me1d988Elnfaoblalroawwiyn.g Ttheicshnriecpaolrtappprreosaecnhtsusetdhetroesmuelettsthoef ptrhoejetcotxicobijteyctsitvuedsy.and describes the
METHODS AND MATERIALS
analysTehse, buisoeldogiincatlhismetshtouddsy,vearse wtehlolse adsesctrhieberdebcyomethnedeUd.Ss.tatEinsvtiircoanlmeatal Protection Agency (1)
I. Test Fish
in thTihsest1u2d0yrvaeirnebowobttarionuetd (fSraolmmoHeg.airLdansesresn)Tr(oluott F#a3r7a38)inusReedd
LBalbufofr,atoCraileisforinnia.ABCThseofftishblveenrdeedrewaarteedranadndmawienrteainfeedd anteAwBlCy
hdaatilcyh.ed Tbhreinleabsohrraitmopry oernviarocnommemnetrcviaasllmyainatvaaiinlebdleon fais1h6-fhoooudr
dtahyolseighdtescprhiobteodperbiyodB.rauhFnishet caull.tur(e2).tecAhnidqauielsy wreerceordbaosficaFlilsyh
-
otbicseravnadtitohnesradpueruitnigc tdhiesehaosleditnregatpmeernitosd,, ailsonigncvliutdhedprionpAhpypleanc--
dix 1.
vere Sreevmeonvteyd-tfwroomhotuhres cbuelftourree tthaenkinaintdiaptliaocnedof4nthethetestte,mpfeirsahwtiutrheouatccfloiomda.tioAnt utneistt. inDiutriiantgiont,hioratiinsbeo,w ttrhoeutfivsehrewerraendhoemlldy pCloancterdol ingrotuhpe dtuersitnigng thniise.studTyhe hardainabomweatnrouwteiguhsted ofas 0.t8h6e m(e2a0s.2u6r)edgatantdheaenmdeaonf stthaendtaesrtd. leTnhgethbioofmas3s9 lo(a1d4:i2n)g rmantewvhaesn 0.57 8/1." These measurements are provided in Appendix I. -
IL Test System
BrungsA (p3r)opaonrdtiaonHaalmildtiolnutMeordelsy4s2t0emsydreisncgreibdeidspebnyserMovuanst uasnedd
ABC LABS #372627
001398
dfiolruetnhte ivnatteerrmiatttoenteaicnhtrtoedsutctcihoanmboefr.FC-F2i0v3e tceosntcesnotlruattiioonnssanodf tchoencteensttramtaitoenriaanld acnodntcroonltrhoelldcotmwpernitsyedfitshhe. teCshtemdiecsailgn.charEaacc-h Ttaebrliesti1.cs, typical of ABC soft blended water, are provided in
controTlhe wadtielrutteor tdheelitveesrtedvesosneelsliatteranofavetreasgte sroaltuetioofn 8o.r0 wtaisnessupfefriciheonutr tooverreptlhaececotuhrese30oflittheer svtoulduys.e wTihtihsinflthoew rtaetset cahacmibrecrusla6t.i4ngtiwnaetserperbadtahy. thTathe wtaessttahqeuramroisatawteircealliymmehresledd aitn 12 21C.
III. Test Material
on SeTphteemFbCe-r2038,(F 12918382-1i)n (gLoootdNoc.on3d0i8t6i)onw.as rTehceeivseadmpflreomva3s4
otbursee.rvedA tdoilbueteransatomcbkersolilquutiidonan(d16w0a,s000stmogr/eld)atwasroopmreptaermepderbay-
dThiessoilnvjiencgtor16v0olgumeofwaFsC-a2d0j3usitned1.t0o dleiltievrerof5.d0eimolniozfedstwoactkert.o
ttwroatilointerresgiomfe
doifluFtCi-o2n03wawtaesr.25,
T5h0e,
r1e0s0u,lt2i0n0g,
nominal and 400
concenmg/l.
IV. Test Procedure - Biological
conduActedpretloimdientaerryminsetaaticconc9e6n-throautrionrarnagneg-efionfdiFnCg-20t3estto wuasse ifnrontheSepdteefmibneirtiv1e5, te1s9t8.8 toThiSseptpermebleirmin19a,ry 19t8e8stusviansg c1o.n0d,uct1e0,d naenidthe10r0. momrgt/alliteyxponsourreabncoornmcaelntreaftfieocntss. werDeurionbgsertvheids attesatn,y lreevperle.sentTahteisvee arnedsulitts vawsereagredeidscutshsaetd twhiethdeftihneitisvpeonsfolro'wst4h0r0omugg/h1 toefstFCw-o2u0l3d. be run at a nominal high concentration of
tionsThheaddebfeiennitfilvoewitnegstthwraosughintihteiataeqduaraifaterforthaepptreosxtimastoelluy4t8o heoaucrhs. teTswtentcyonrtaaiimnbeorw. trOobusterwvearteioinmsparftoirallmyortdailsittryibutanedd s96ublhoeutrhaltesrtesppeornisoeds. were made once every 24 hours during the
V. Test Procedure - Chemical and Physical
measurTeedmpeirnattuhereco(ntCr)o,l,dilsosvo,lvmeidddloexyagnedn hi(mggh/els)t, coanncdenptHravteiroen Tchoensteainriensgultssuravrievipnrgovfiidsehd aitn A0p,pe4n8diaxndI o9f6 thhoiusrsreopfortt.esting.
ABC LABS #37242-8
001393
VI. Statistical Analyses analyzTehde (r4e)suflotrs 2o4f, 4t8h,e 7d2efainnditi9v6e-hosutrudLyCsw)ervealusetsatainsdtictahleliyr pceorrfroersnpeodndionng an957IBMcoPnCf-iXdTencceompluitmeirtsu.sinAgLPt'hcealncoumliantailoncsonwceerne LEergagtioonrs95o7f FcCo-n2f0i3d.enceBeclaiumsietsnwoermeortdaelteirtnyinoecdc.urred, neither an
RESTLTS withinVattehre scpheecmiifsiterdy lpiamriatmsetefrosr cmoenadsuucrteidngataqu0a,ti4c8 taonxdici9t6y htoeusrtss w(e1r).e D7i.s8so(lTvaebdleox2y)g.en Prearncgeendt fwraotmer7.s2attuora9t.i0onmgo/fl,diasnsdolpvHedraonxgyegdenfrgomive7n.3thteo a1b3oCv,e rmeesapseucrteimveenltys. ranged from 70% to 892 |at temperatures of 12 and 48-, F7r2o+n atnhde Sd6a-thaourcoLlClse,ctevdaludeusriwnegretheisstifmlaotwe-dthtrooubgeh >sit0u0dyu,g/lthe(T2a6b-l,e 3a)n.y" Aesxposshuorwen ilnevTealbleor4) cnoontmroorlt,alitFyoror tahbinsormraelsseofnf,ecttshewermeo seeefnfeactt c40o0ncmegn/t1r,atitohne mfaoxrimtahecoanccuetnetrattoixoincittyestoefd.FC-203 to raisbow trout vas PractTihcee sRteugduylavtaisoncsond(u5c)teadndfoltlhoewipnrgeltihmeinianrtyentreopfortthevaGsoodreLvaibeowreadtorbyy AorniagliyntailcalravBidoa-tCahewmiilsltrbye pLraobvoirdaetdoriteos'3K QCuoamlpiatnyy wAistshurtahnecefinUanlit.reporAtI,L vith a copy retained at Analycical Bio-Chemistry Laboratories.
ABC LABS #372629
001400
TABLE 1 CheatcalbyCAhBaCr'asctAeqruiasttiiccsToofxicSoofltogyBlDeinvdeidsioWnager Used
Parameter
Temperature Dissolved Oxygen oH Hardness (CaCO) Alkalinity (CaCOy) Conducttvity Suspended Solids Aluntnun Arsentc Boron Cadatun Chrontun Cobalt Copper Tron Lead Mercury Nickel Silver zine
Concentration
18 - 23% 8.0 - 9.3 mg/1
71-79 40 - 48 ng/1 4b - 56 mg/1 100 - 160 ymhos/ca 0- 1.4m
<0.2 ug/L <0.001 mg/1 0.181 ng/1 <0.005 mg/1 <0.010 mg/1, 0.169 mg/1 <0.025 mg/1 0.127 ag/1 <0.003 mg/1 <0.0004 mg/1 0.048 mg/1 <0.001 mg/1 <0.020 mg/1
"ABBlCendweeldlwvaatteerristoaamcihxiteuvreeaoffirnaelverhsaerdnoessmsosiosf b(eRt.0w.e)enand 40 - 48 mg/l. Paster aeration tNoitmee: perTihoed rAapnrgiels tohfrotuhgehseJusnee,lec1t9e8d8 poarrafmreotmertshearmeostfrormecetnhte ~~ qtuhaersteerrlayngesscraereen ofnorfilceonattamAiBnCanLtasb.oraAtLoLriersa.w data supporting
ABC LABS #37262-10
001401
TaBLE 2 TWoaxtiecritQyuTaelsittyoMfeaFsCu-r2e0m3enttosRDautrnibnogw TtrhoeuAtcu(tSealmFologwa-iTrhdrnoeurgih)
ConcNeonmtirnatailon (ag/1) control 25 50 100 200 400
Tohour
Water48-Qhuoaulrity
ETT
Tceap.mDl.0. pf Temcp. Dm.g0/.l pi TemCp. mDa.0/.i pn
13 8s 77 13 7.7 8 13 89 7.4
1386 77 13 S77 27 13 9.0 7.4
139.0 77 13 7.9 77 13 83 7.3
139.0 7.8 12 7.2 7.8 13 8.6 7.4
#546)a.solved oxygen concentrations - Dissolved Oxygen Probe (YSI Model
PaHnd-oVpHmePtreorb.e (Corning Model 476182) used with a Corning Model 125 pH NOTE: A`ltetmipteurdaet-uardesjusotfed12diansdsol1v3edC o1xsyg1e0n.3saantudra1t0i,1onmga/tl,therestpeestctively.
ABC LABS #37242-11
001402
TABLE 3 The ETstoixmiactietdy SLCtga)dyVoaflueFsC-w2i03thto95%RaCionnbfoiwdeTnrcoeutLi(mSiatlsmofgoarirtdhneer9s6)-Hour
Pertod HoofurEsxposure,
" 50
24, 48, 72, 96
>400*
(ag/1) 95% Conftdence Limits
-
"LNCog,morvtaaslictoynsoidcecruerdredgraetatearnytehxapnostuhreemacxoinmceunptreaxtpioosnu;re tchoenrceefnotrer,atitohne. No onfidence linits could be determined.
ABC LABS #37242-12
001403
ns 4 oeeei eeTaat-- atantfe-- sToon
cmeSraprIeion
a etos rotons stones si"nie tort, Shortens =nmSsevaonsoSmn thsevstons oomm,. Soerastns
weer mo wn 0 mx 0 mx 0 ax
sm 0 wx 0 mx 0 mx oan
wm 0 mx 0 mx o mx o ax
wm 0 wx 0 mx 0 mn o wx
mm o mx 0 ax o mx 0 ax
:
wm 0 mw 0 mx 0 ax o mx
Eo ovomctons 1etom
ie2g222
LiTeRaTURE C1TED
(1) wC(iCo.tmhmiE.ttFeSietsehp,ohnanM,MaecCtrhhoaoitdmrsvaearnfyto.erbrTa1ot3xe7is5c.itayMnedtTheosAdtmssphfiwobiritahAncsu.AtqeuaTtEoinxcvticriOcorywgsaTeneniscstamsls APprortielct1i9o7n3", A6g1enpcey, Ecological Research Series EPA-660/3-75-009,
(2) BoafrnaduRheBnsl,euasJrg.cihlLl.Fsi.asnhd: RE.nRvaAii.rnobmSoacwehaotTeartlotugteP,rr.oFtaetc1ht9e7ia5od.n MAiAgmcenqnoucviyss,,itCiEhocanonlnaoengldicCCauallttfiuRsrehe-
search Series EPA-660/3-75-011, May, 1975. 45 p.
(3) Mtouusntf,orD.FisIh. TaonxdicWo.loAg.icBarlunSgtsu.die1s9.67.WatAerSiRmepsl.ifLi:e2d1-2D9o.sing Appara@ S1t9e7p8.han,A Cc.omBp.u,terK.prAo.grBaunscfho,r R.calScauiltaht,iJng. aBnurLkoegga.nd UR..S.W. EAnnvdirreown.:
mental Protection Agency, Duluth, Minnesoti. pre-publication
`manuscript, August, 1978.
(5) OGrugiadneilzianteisonfofrorTeEsctoinnogntocf CCohoepmeircaatliso,n ParnidncDiepvleelsopomfentG.ood 1L9a81b.oratOEoCrDy
Practice Annex 2, C(81) 30(Final):7-28.
ABC LABS #37242-14
_
001405
quay Assuaice staTniENT Quality Assurance Statement for preliminary report #37242 encicled,
"Acute Flow-Through Toxicity of FC-203 to Rainbow Trout (Salmo gairdneri)", for Mo Elnabarawy, 3M Company, St. Paul Minnesota.
7a, avcortesce, with A30 Labezstories' futene that sll srulies soe
ducted at our facilities are designed and function in compliance with
Ee EE good laboratory practice i regulations and the Lmn arL re=e: protocols for individual CE Brrooacneidoumrse apurdiiotr vtaos tcheo.ndiuncitteide atoinonOcotfobetrhe 13s:ted1y9.38inwgiitchateesdeitehenyecwaerpesS .rcien 143101 Tdaabtoe erat"aonrdiee sse:comnadnsag"eomnenNtovoennbNeorveTmovyer 119;3819"8i6n:dicAatebdinr etdhati.nestphaeceeisoenpoovfe prebrrueRevvg egitim Boligne gr alle Boog iri
conducted by ABC Laboratories.
Soe yur fivae 9e 57 Qeniiens Sletinm g to the SOfEeLiohTpraaiieed
contact the Quality Assurance Unit at your convenience.
TJonie TtL G?TentiGaaatste lH T1er2e-087
Quality Assurance Officer
ABC 001406 LABS #37242-15
STUDY COMPLIANCE STATEMENT
TSthurdoyughComTpolxiiacnictey SotfateFmCe-n2t03fortoABRCairnebpoowrtTr#o3u7t242(Seanltmiotlgeadi,rd"nAecruit)e",Flofwo-r Ho Elnabaravy, 34 Company, St. Paul, Minnesota. city Itnesatsccocrodnadnuccetewditbhy AoBuCr Lfaabcoirlaittoyriefso'lloiwntgeonotd thlaatboraaltlorayquaptriacctitcoexsi,wAaBsC'sconsdtuucdtyeddiirnecctoomrplfioarnctehewiatbhovPeritnecsitplheesreoifn GcooondfiLrambsortahtaotrythPeracsttiucdey Annex 2, C(81). retaiAnLeLd aotriAginnaallytircaavldBaitoa-Chweimlilstbrey pLraobvoirdaetdortioes3.4 Company, with a copy
fue 1. Bowanan C Study Director
12-2-f
Date
ABC LABS #37262-16
001407
