Document RpOawk7reNjKoxgJkL9KBx5QV
MICROBICS MICROTOX@ TOXICITY TEST
TEST SUBSTANCE
Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, or as a component ofFC-3003. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003. Currentinformatioinndicatesitisa mixtureof I% PFOS, 15% diethyleneglycolbutylether,79.39% water,2% Sultonefoamer, 1.5% sodium octylsulfate1,% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotdazole.
The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample.
MET
Method: MicrobicsMicrotox@ "BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1997 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearregression program providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-QTM water Test temperature: Not noted. Stock and testsolutionpreparation: A 1 g/L primarystocksolution was prepared bX dissolving100 mg oftestsubstance in100 mL of MilliporMeilli-Qwater. The pH ofthe stocksolutionwas then adjustedfrom 5.7to 7.7 using1.0N NAOH. An osmoticadjustment was made using200 mg NaCi dissolvedin10 mL stocksolution. Appearance oftestsolutionwsas noted as "cleaC'A.lltestsolutions were made by proportionadlilutions. Exposure vessels: 4 mL cuvettes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESJLTS
NominalconcentrationBsl:ankcontro6l2,.5,1252,50,and 500 mg/L
Element value:
5-minute EC50 = 311 (289 - 335) mg/L 15-minute EC50 = 171 (161 - 181) mg/L 30-minute EC50 = 120 (118 - 122) mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacteriumphosphoreum was determined to be 120 mg/L witha 95% confidenceintervaolf 118 to 122 mg/L.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
Reliability:Klimischranking= 2. The studymeets allthe criterifaorquality testingH.owever, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmatioonftheamount offluorochemicalproportioninthe solution.
REFERENCES
This studywas conducted by the3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number R1331, 1997.
OTHER
Last changed: 6/26/00.
ENVMONMENTAL
LABORATORY: AUCROTOX PREP SHICET
TESTSUBSTANCE:
F'c-3 C)OB N-IFFV:P-,.o.5T9, PkL IPA
LR: Date.* Arwyst:'Tvs
C-CONDo (zo
1. Prep&-radonofStock Solution
MassIVot TestSubstance.
Fbud LWudon Volume.-
*Dih=r
M"
Is% @L@,
Fmal Concenuadon ofStock Solution:
L
2.pH Adjustment ofStock Solution
InitialpHofStockSolution: S.-7
pH.4 djustedto:
-7.-?
pHAdjiistedwidl-
< ( c@re,
oD
3.Osmotic Adjustment of Stock Solution
OsmoticAdjuster.
Commma:
kovlk
C)v
IA2CtS
4.Appearance ofpH and osmoticallyAdjustedStock Solution
OP.4QUE (circloene)
color.-
Q@leckx,
Comments:
S.Comments
2- 0
MICROTOX DATA REPORT
Basic Test
FILE: R1331-2.K5 Second Run FC-')'00@)' AFFF
(Australia) 3--13-97 TKS
Test Time: NI]MBER
b rr.-intite--, 10/IT
CONC.
Osmotic CR/GAMMA
Adjustme!il-..Nt@(.',l
% EFFEC:T AV
q I)IP'P.
C<-)ntrol
96.39/ 84.,)8 98.56/ 8 1
1
93.56/ 7().36
2
92.15/ 70.94
Q..1
99.36/ 64.@,7
4
98.54/ 63.'70
5
94.67/ 48..@8
94.86/ 4"1 .@)7
7
102.12/ 29.35
a
99.19/ 30.22
0.0 ().0
611.@.5000 62.5000 125.0000 121@-).0000 250.0000 11!5(()).000 500.0000 500.0000
0.8'(23 0.844":;
0.141- # 0.115 4 0.321 # 0.328 # 0.679 # 0.711 1.986 # 1.817 9
12.4 io.,:,@
11.3 q 1.0
'@4.'7
24.5 q 0.2
40..'.)
4 t .k"
41
0.
66.tl
E',,.l
1,
1 t.1
CR
Co(.'ORRP:(.I'I(-F.A)CNTOR, - 0. 8,1)83
# Used for calotilat.ioiis
F.(',@)3011.0 mg/L (95% (,'(-)NFIDENCREANGE:289.0 ']'(3::)34.7)
S.tffziattire CON'RIN[JED TO NEXI' PAGF ...
TEST DATE: 3TIME:
-2-
FILE:Rl331-2.K5
PAGF :2
10 ZDDDDDDDDDDDDDI)DDDDDD-r)DDDDDDI)I)DDI)DDDDDDDDDDDI)DDDD.L),)DDDDDI)DI)J)' o'SLOPE 1.2796
3
G
A
m3
m3
A3
8
3 6
3
4
3 EC50
2
.1o (.-,jDDDDDDDDDDj)D.DDDDDDD-DDDDDDDDD.r)DDDDDDDD-DDDi)DDDDDDDDDDDDDD-Dy
10
100 CONCENTRATION 1000
13
<
3 3 3
3 3 3 lOE4
ESTTMATINCT EQUATION: LOG C -: 0.7769 x LOG b
95% CONFIDENCE FACTOR: 1.07603 FOR EC50
COEFFICIENT OE' DF'PP:RMINATION: R-2
0.99416
+2.4928
FILE: R1331-2.Kl5 Second Run FC-3003
MICROTOX DATA REPORT Basic Test
AFFF
(Australia) 3-13-97 TKS
Test Time: 15 mitiutes
Osmotic A(J,itistmentt4,iCl
NUMBER
IO./TT
------- ------------
Control 96.39/ 86.73 ("Olitrol- 98.56,/ 87.78
CONC.
t'JP/GAMMA @k FFFECI' AV %
----------- ---------- -------
0.() ().0
0.8998 0 0.8906 #
DIFF.
9:@).5 6 64.20
6'-1.5000
0.305
2
92.15/ 65. @19
62.5000
0.263
99.36/ 57,.26
121-).0000
0.670 #
4
98.54/ 51.49
125.1)000
0,713 #
f@
94.67/ 3 i".8':@
@150.0000
1.581 #
94.86 '/' 33.21
25n.c)ono
1.557 4
7
102.12/ IS.73
500.1)()Ot) 4.812
8
99.19/ 15.38
500.0000
4.773
40.1 41.@',
9 8-9.8
1 .2 40.9 q 0.8 6 t. I q 0. 82.7 q 0.1
CR COCORRECTI"ON FACTOR = 0.8952 A Used for oalculatioris
EC50 170.8 mg/L
CONF.Tj)EN(,b:RANGE: 161..0 TO 181
Signatur CONT!NRJED
67N rO NEXT
v
PAGE ...
TEST DATE: -13
TIME:
PIS
y@,)2.
FILE:Rl:331-2.Kl!') E@AGE
t3 -c-1-7 S
io L?DI)DDDDDDDDDI)DDDI)DDI)DT)Dr)DDDDDDDI)Dr)1)1)])DDDDI)I)T)DDDDDDDDDDDL)I)fyl)D?
.YSLOPE 1.1826
3
3
G
A3
3
m3
m3
.3
A
3
6
3
4
3
3
3
3
3
3
FC50
3
.10 CIVILI,1)1)1)1)DDDDDDDDDDDDD-DDDD.i@DDDDDDDDDDDI)O'Di)I)DDDDI)f)I)I)f)DDD.I)DDDY
10
100 CONCENTRATION
1000
lOE4
ESTIMATING EQI)ATION: I,OG C = 0.8430 x LOG b +2.2325
95% ('ONFIDENCE FACTOR: 1.06065 FOR FC,')O
,,"OED'FICIENT OF DE'I'ERMINATION: R^2
0.99691.
MI(3ROTOX DATA REPORT
L)
Basic Test
FELE: R13:11-2.K30
@iL,-o.oiiRduzi F(@'-3 0 0')' AFFF
(Australia) 3-13-97
TKS
Timf--: 30 minut.e-.@,
Osmotj.(:.A-djust.meiit:NaCl
tqumBER
I()/Ilr
CONC,
CR/GAMMA
EFFECT AV % I)IFF.
96.39/ 93.39 C,otitrol. 98.56/ 9'1-3!-)
1
93.56/ 62.39
92.15/ 62.84
3
99.36/ 46.35
4
98.54/ 45.93
5
94.67/ 26.50
6
94.56/ 26.61
7
102.12/ 11.29
8
99.19/ 10.50
().0 ().0
62.5000 6',-1.5000 125.0000 125.0000 250.0000 250.0000 500.0000 500.0000
0.9689 0 0.94'71 #
0.437 0.405 # 1.054 # 1.055 # 2.422 2.415 7.665 8.050
30.4 28.8 51.3 51.3 70.8 70.7 88.5 89.0
29. F) 0. 8 51.3 q 0.0 70.7 q 0.0 88.7 q 0.2
CR = COCORRECTION FACTOR 9 Used for calculations
z 0.9580
E(,*@DO1,-'0.3 mg/L (").5%CONF'IDP,N(7,E RANGE.
TI) !::,:.41
Signaturt@_,-@@s@r@@@
0,/-
1
CONTINOED TO NEXT PAGE...
TEST DATE: TIME:
Z3-7
FILE:RI331-2.K30
PAGE 2
10 ZDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDI)DDDDDDDDDDDDDDDDDDDDDDDDI)?
.roLOPE
1. 3811
3
3
--
G .3
A
I?
m
3
m
A3
4 3 3
3
F@C50
3
10 c-)DDDL)I)DDDD1)L)DDf)f@[)I)DD- D. DI)DDDDDD-DDDDDDDDDDDDDDDDDI.)DDDI).[)DD-r)I)DY
I ()
100 CONC',ENTRATION 1000
E,;,riMA'rTNGEQI)ATION: LOG C = 0.7240 x LOG b
9b% CONFIDENCE FACTOR: 1.01758 FOR EC50
i'OFF'P*lc,'TFNTt')FF)FTP,'RMINATIC)N:
0.99999
+2.0802
3 3 3 3 I? 3
3 3
3 1 (rF-.4