Document Rp0qr8DKpxMNaEjYgXKjNOXMv

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber isnotnoted. The testsample is FC-603EF. Currentinformationindicatesitisa mixtureof 1.30% PFOS, 10% diethyleneglycolbutylether,54.15% water,22.5% ethyleneglycol,2.6% propanesultonefoamer,5% Alkylethersulfate0,.1% N-(3Chloroallyl)hexaminiucmhloride0,.9% Xanthan gum, 0.9% starch,1.0% triethanolaminea,nd 0.05% tolytlriazole. The followingsummary appliesa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the tluorochemical component of the testsample. METHOD: Method: OECD Guideline203. Type: Acute static GLP: No Year completed: 1997 Species: Pimephalespromelas Supplier:In-houseculturemsaintainedatAScl-DETD, Duluth,MN. Analyticalmonitoring:Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: LC50 values were calculatedby the Trimmed Spearman-Karber method. The NOEC was calculatedusing Fisher'sExact Test (Toxstatsoftware,Version3.5). Test fishage: Juveniles Length and weight: Average length 12.7 mm Average weight 0.0165g (wet) Loading: 0.066g/L Pretreatment:None. Test conditions: Dilutionwater: DechlorinateCdityofDuluth,MN, tapwater(post carbon filtetrh)atwas aeratedfor24 hours priorto use. Dilutionwater chemistry: Hardness: 48 mg/L as CaC03 Alkalinity: 46 mg/L as CaC03 pH: 7.80 LightingC:ool-whitfeluorescelnitghtsA.dailpyhotoperiofd16 hourslight and 8 hours dark was maintainedthroughoutthe testingperiod. Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasisand mixed by mechanical stirring. Concentrations dosing rate: Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 4 L glassjarscontaining2.5 L testsolution. Number of replicates:2. Number of fishper replicate:10. Number of concentrations: fiveplus a blank control Water chemistry during the study: pH range (0-96 hours): 7.73 - 7.83 (controlexposure) 7.31- 7.85(1000 mg/L exposure) Temperature range (0-96hours): 21.1- 21.3 OC Conductivityrange (0-96hours): 133 - 149 pmhos/cm (controelxposure) 150 - 159 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 7.1- 9.2 mg/L (controelxposure) 6.7- 9.4 mg/L (IQOG mg/L exposure) Remarks: Solutionswere aeratedcontinuouslyduringthe exposures using singlebubble aerationto minimizefoaming. RESULTS Nominal concentrations: Blank control,1,130,216, 360, 600, and 1000 mg/L Elementvalues: 96-hourLC50=>1000mg/L 96-hour NOEC = 1000 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determined tobe >1000 mg/L,the highestconcentratiotnested.Mortalitoybserved atthis concentrationwas 15%. The 96-hourNo Observed EffectConcentration(NOEC) was determinedto be 1000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabilityK:limischranking= 2. Thisstudymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES Thisstudywas conducted by AScl Corporation,ASci-DuluthEnvironmental TestingDivisionD,uluth,MN atthe requestofthe3M Company, Lab Request number P2581, 1997. OTHER Last changed: 6/26/00 AS,:lCurlx)rmtit)n/ASCI-DuiuLh EiivironinenTteasltingDivision ASci-DETD StudyID# 5010-069 STUDY TITLE ACUTE TOXICITY OF FC-603EF, LIGHT WATER(T,',I) ATC 3%, AR-AFFF TO FATHEAD MINNOW (PIMEPHALES PRO.4fELAS) DATASTANDARD OECD GUIDELINE 203 STLIDY CO@NIPLETED Janitar;28, 1997 TESTING FACILITY ASCI Corporation/AScl-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# PI-1581 Sponsor. 3M Company Sponsor Study ID# P'-1591 ASt:lCtirF%)r;itiiin/AS@f-Dulutn En%iruiiincnTLca%liisii; AS,:I.DETDStudy[Do 5010-068 1.0GENERAL INFOITAIATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand Report Acute Toxicityof FC-603EF LightWater(TM) ATC 3%, AR-AFFF to FatheadNtinnow (Pimeplialepsromelm). OECD Guideline203. 3M EnvironmentalTechnology and SafetyServices,Building 2-3E-09,935 Bush A%-eiiueS,t. Paul,NIN 55133. Susan Beach, 3NI EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St. 13aul,NIN 55133; Tel No. (612)778-7452. P258 1. AScl Corporation/AScl-DulutEhnvironmentalTesting Division,4444 AirparkBoulevard,Duluth.SIN 55811; Tel.No. (218)722-4040. Todd NlcGuire. Joe Amato. Donald NioUni. January21-25, 1997. Alan Mozol Re )OrtSionature- Retentionof Raw Data and Final Two yearsfrom thedate studyiscompleted. Report L=ation of Raw Data and Final Report AScl Corporation/ASc-lDuluth EnvironmentalTesting Division,4444 AirparkBoulevard,Duluth,MN 55811; Tel.No. (218)722-4040. Sponsor; 3&1 Company Sponsor Study [D# PI-581 2 AScl CorpomiionA/ScI-Duluth EnvironmcntaTlebtingDivision ASci-DETD StudyIDO 5010-068 2.0OBJECTIVE(S) To determintehe96-hLCSOand96-hNOEC (noobserveedffecctoncentratioofnt)hetest substancFeC,-603EF,forfatheamdinnow(Pimephatpersonielausn)derstatitcestconditions. This studywas conductedaccordingto the AScl studyplan retainedby the Sponsor and OECD Guideline203 (OECD 1993). 3.0 TEST METIIODS TestSubstance Test Organisms DilutionWater Properties:FC603EF, amber-coloredliquid,specifigcravityca. 1.06 (water= 1),vapor pressureca. 15.8mmHg, stablea,nd completely solublein water. Testconcentratiotu:0 (dilutiownater control),130, '-1'6, 360, 600, and 1000 mg/L. Each testsubstanceconcentrationobtainedby mass a@lditions todilutiownaterfollowedby mechanicalstirringT.hese solutions splitintotwo aliquotsto accommodate testreplicates. Source: Healthy,juvenilefatheadminnows (Pimephalespronields)obtained from AScl-DETD in-houseculture.The batchof fishused inthestudy was hatchedbetween December 18 and 20, 1996. Holdinglacclimation:Held and acclimatedfrom hatchinguntiltest initiati(oanpp.32 days)inthetestdilutiownater.For the48 hours precedingthetest0,% mortalitoyccurred. At testterminationt,he fish were 12.7 2.4 mm in mean lengthand 16.5 8.9 mg in mean weight. Use inrest:Used 10 organisms/replicaatned 2 replicates/test concentration. Source: DechlorinatedCityof Duluth,MN, tapwater.The most recent chemicalanalysisisin Raw Data Package. CliaracteristicAst: testtime,the water had a hardnessof 48 mg/L (as CaCO3) and a pH of 7.80.Aeratedfor24 h priorto use inthetest. Sponsor: 3M Compary Sponsor Study ID# P2591 3 Exposure Chambers Incubafion Observations Endpoint Calculations ASci Corpomtion/ASIC-Duluth EnvironmentaTlestingDivision AScl-DETDStuJyID#501a@068 7@pe:4-Lglasjsars. Use in test:2.5 L of dilutionwater or testsolution/chamber.Solutions were aeratedcontinuouslyduringtheexposuresusing singlebubbleaeration to minimize foaming. Duration: 96 h. Dailyphotoperiod: 16 h lightand 8 h dark, maintainedusingcool white fluorescenltamps. Temperaiiire:21.0* I*C. Biological:Daily- Test or.-,anismswith abnormal behaviorand signsof stressa,nd dead testorganisms. Water cheinisin:(1) At testinitiationt-otalhardnessand alkalinitv(,2) daily-temperature,DO, conductivitya.nd pH. All determinationswere made accordingtoAPHA methods (1995). LC50's: Trimmed Spearman-Karber Nlethod. NOEC: Fisher'sExactTest (TOXSTAT statisticsaolftware,Version3.5, West Inc.,and Universitoyf Wyoming). 3.0 RESULTS TestSubstanceToxicity(Pertinendtataarein TablesI and 2) 24-h LC.50: > 1000 mg/L (Not Calculable). 48-h LC50: > 1000 mg/L (Not Calculable). 72-hLC50: > 1000 mg/L (Not Calculable). 96-hLC5o: > 1000 mgCD/L (Not Calculable). 96-h NOEC. 1000 mg/L. Sponsor: 3&f Company Sponsor Study ID# r-591 4 Rangesof Water ChemistryParameters AS%:lCtjrfx)ratjL)n-iDAuSliu:tlh Envtrt@nmentTa"lting Divisiun ASci-DETD StudyIDS 5010-068 Totalhardness(asCACO, mgll): 48. Alkalinit(vm CaC03 mglL,):46-52. Temperature(*C): 21.1-21.3. DO (ingIL):6.7-9.4. pH: 7.31-7.85. Specificconduciii-i(rWvnhos,cin):133 1591 4.0TEST QA/QC QA/QC Critena Mortalityin controlexposure must not be more than 10%. The lowest DO concentrationa*,test terminationmust be at least60% of the air saturationvalueat testtemperature. Test durationmust be 96 h. Reference SubstanceToxicityTest (thistestis optional). Data 0% mortality. The lowest DO concentrationmeasured was 75% of the airsaturationvalueat 21.0'C. The testsolutiondissolvedoxygen concentrationswere maintainedvia single bubble aerationforthe entiredurationof the test. The testdurationwas 96 h. Controlchart fnr sodium chloridereference toxicitytestingisin Raw Data Package. Sponjior;3M Company Sponsor Study ID# F"-581 5 ASci Ckirix)rAtiLA)Sicti/-Duluth Eii,.in)nnientTAcl.%IiniD;ivisiosi AScl-DETD StudyID# 5010-068 S.0REFERENCES American PublicHealthAssociation(APHA). 1995.StandardMethods fortheExaminationof Water and Wastewater.APHA, Washington,D.C. OrganizationforEconomic Cooperationand Development (OECD). 1981/1993.OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformationCenter,Washington, D.C. Hamilton,M.A., R.C. Russo,and R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatingMedian LethalConcentrationisnToxicityBioassays.Environ.Sci.Tecliiiol1.1('/): 714-719;Correction12 (4):417 (1978). West Inc.,and Universityof Wyoming. TOXSTAT Wyoming. Soft%%-arPeackacye.Version 3.5. CheNcline. Sponsor:3M Company Sponwr SUAJY ID# P2591 6 t AS,:iCorporation/A&-i-Duluih EnvaronmcnialTeiiiingDivision ASci-DETD Study IDN 5010-068 TABLE 1. ACUTE TOXICITY OF FC-603EF LIGHT @VATER(T.Ni)ATC 3% AR-AFFF TO t FATHEAD MMOWS (P.promelas) Test Substance Nominal Concentration (mg[L) 0 (control) 130 216 360 600 1000 Percentages are in parenthesis. Cumulative Numberof Dead Organism and Percent MorWit3r4 24 h 49 h 72 h 96 h 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) o(o) o(o) 1 (s) 0(o) o(o) 0(0) 1 0(0) 4(20) 1 (5) 2(10) 0(0) 3 (15) EFFECT AND NO-EFFECT CONCENTRATIONS %N'ITII CORRESPONDING 95% CONFIDE-%CE INTERI'ALS LC, o and NOEC 24-h LC50 48-h LC50 72-h LC50 96-h LCSO 96-h NOEC Data (Mg/L)b > 1000 > 1000 > 1000 > 1000 1000 95% Confidencelnt,!7al---] Not calculable Not calculable Not calculable Not calculable Not applicable b LCSO values were calculatedbased on testsubstance nominal concentrations. Sponsor: 3M Company Sponsor Study ID# P2591 7