Document RjpqQLgdv0834mb482D34nJNv

FILE NAME Quebec Asbestos Mining Association QAMA DATE 1952 Aug 1 DOC QAMA062 DOCUMENT DESCRIPTION Saranac Lab Report to QAMA - Asbestosis and Pulmonary Cancer with Several Cover Memos Mr. Ivan Sabourin Aldred Building Montreal Quebes Canada Dear Ivan Thank you very much for the copies of the first report of May 7 1952 covering the work done from Saranac on to date for the ASBESTOS Q. A. M. interin interin AND PULMONARY CANCER A. on this subject to mention that you and Carmen vould You were thoughtful happy to have Hervil and me see you home have been most pleasant oms opportunity that presents itself to tumately I don't know at this time be there Our short visits at your We shall take the very first see you tuo again though unforjust when we might be up that way we do hope you will rake the point see and stop with us and, We both appreciate very much your suggestion that we come be in meantime should either or both of you in this area the to With were personal regards and very best wishes to you both m Sincerely yours T + Ss. J. P. Woodard VE ee ert EPRI ORT 1s + Ser er 1952 AA ARE 4 - . : & -~ rey ng ta { - . . , Ls no ARO on AN tee a mg ek IS - - atoe , , e ae . . 1 we? .. St ~ , x* 2 rentone fen Sneek tae. aoe NO . wes a ey fof wde ~ .* , ae , : . * % : etapa t a we oe ey dy pow oem, AeBA sate opran Mudine we NAL Pre orm -. , t ea a ty * ? 24 ab: Sit . Te SOeaed at was 4 J ads * i|q 0: vt, + | ele 7% : -. . .y,ee ry 4 Mudinine Mudinine 4 oe Dim sinettyriaBlolisvus oy a York Dim Motionl Motionl { Se eye 4+. 4 InstituteFirst Jd rs . \ Yor1k 6 sity ory ros, York , YokYok "ye Ba New age 3 ANS of peta leter 10a" ene wes At va + A Dear on 8 bestosia bestosbieastosia julmonary interia is aera andjulmonaryjulmonary interia study that exe aewp bestosia : a, . | attached firstthitshis , -. . . au 19572 of is 7 1957 oon n t isis Me tefe: 6a", 2 e : . ; = = eS 7 t . = . , reg yt an Pos een Ce re change animatlherunnfawvorsabluenfavoreblxepHoesleunreeMfeinadlings three 8, neEeNB ARICA 8, exposre eb7eyesoaned! tfhourteen conclusions coalusiong ScoaluRsiong Beak ef - 4 - You . a 4 You of animal oA , +, ? eee e ^' . ek a) a a, not ind m te7 . + that the do - findings ' course lata cooalusiong on ry vits* 7 - AR oe coatem d x, 4 c > : + 4 oh af ee - < fe o> sey sod % War. tee eg we Lt oo 7% ee Se _- . 4 ae ie , wae ee ' : th e le a : age ey@-! o . z Mea 2 Rhy + 7 a ge oy \r ee .- a Nad 2 2 on s . ae See 4 . "op Sanat ot _ A . aye igh Cie wy, : . gs ~ . yak Set oe Ro .. ; ; ~ Herbert HerbertF. mS zy ne eens . roe y / . tede bos Herbert Ball OR bos * one xtia1 eeea Herbert Ball one one x . a eaSy vf tw Az % - R 7 fo = . ; Z: er et. aie ir Pa es ige es ae f *e ve . a f , 2 Sorte I oY wa a ) net raing i . at . ; a wo. Es weg Sare ae a . . oe < "9 ay we 2 .Y '. 7? oo- . aae aa a {[.- r: . yA Me . 4 .v ad c eo . _ . . oy . . . mire . ra af * se NM THE TRUDEAU FOUNDATION pm: . FOR % ; ~~ *, . , - THE oan ~t N.T. a BARAMAC Fae AT BARAMAC LANE N.N.TT. . N.T. CLINICAL . x, AND EXPERIMENTAL ee - Sah oS soe we . " i re . STUDY OF PULMONARY DISEASE ap4 . - mea sete hee ed ae we! THE a AY TRUDEA N.U Y. . DEPARTMENT OF PHYSIOLOGY ee wo. vk * 2 a ty, THE SARANAC LABORATORY j 2 THE TRUDEAU FOUNDATION T+ SCHOOL a THE TRUDEAU . = - * . Sr aa : a | - . ; : Nes rr: 2s mo a8 - ms - : . | ~~" THE DEPARTMENT OF BIOCHEMISTRY / RADIOLOG ZC. THE OFPANT Far - q . THE TR THE bs an ORATORY . f ne > 4 ae Nee ve . peas . et Tm, ee 6, e "se SE a ne eas ~ as Mena ce se oS iad = YR Ny ~ Pa Sn 2 ee len Oe oe ae -% ws? io ye, ot ; 7 any ee? pear Wr. Sabourini ee a Tp 2 : wy tee Be ae . te me baal PT ow . O ee RO. tem we . . ened ad - ne we deta ee q Be-3 | ~ a6 -OG ~. tt RR: . oe - Se Nat ie) . 8 on 7 nan ere ot 7 or CANCER which study is Mining Association is alling on Timrsday let us know the member owhom they are to be being supported by the Quebec Asbestos and vill beready for in preparation week Will you please this Friday of needed of copies that will be and eat ete . ge . ft = -- . TE | Ree. . mes 40 (A wrt eR -E~ SP. . . AO Ni 5 Sincerely PR Sr yours wae o 2+ Me _- Hillim 1. Nine Fre A.3 L. ae NN a oe ln an ~ ade v 83ane ae a an ' _* te Pearetary to = 7 Executive J. we we ws -&, Pearetary . Director sole r ~ ~ sts ; te Mae Tee . wt ~ , &, woe ie if ~ we \ . gSa ?- wt ; - CO Mr. J. P. Woodard + ry ~ . KEdye. A ot } Se a Ns. Tee fe FR oe . Soe as) 3 wp Rp he rr. a 2 os oS one . * ae .- ts a: 4 ener ware & i . . tee OF + - , ie 4 Ve mew 3 ware arb . ttee ! y _ 4 - a. I Ses . eo re eg! ePtia kae! + won hae? a . ; ner: . cole ra ar @ 7. ae : . . * rd & CO LE : . L . <7 ' x . os : A Cw ame oenak -* Re . . . oN os o ~ ~~ wae - OT i . Mes en ae vor 3 1c , . . x oem o Be, Aes . - . . aan r yours m* . . . J. P. Woodard ~ * , a Sars mb . sm Pee .F Ty . os oO. - Ee EP pee | oe . wv mae RT * ~ Pedy os was a. i re | . s ee ae e . t vee us , vrs bd oe so. t , IVAN SABOURIN ADVOKATK ADVOKATK ADVOKATK Paige Woodard Esq Executive Offices Manville Corporation 22 East 40th Street New York 16 N.Y. Montreal July 9th 1952 E - Dear Paige Re Saranac Asbestosis and Pulmonary Cancer First Interim Report I was away for a couple of weeks and regret much delay in forwarding you the five copies which you will find enclosed Carmen and I hope much to have the pleasure to see you and Mervyl soon at the house There are many things to talk about 10/18 encl 5 Cordial regards , (4v tae : see Ivan Sabourin ^' we hp Pirct borin Report 4 14 00 Cte seme Pe te he te tr rene toto to to Asbestos AsbestoAs sbestos Asbestos Asbestos Asbestos Asbetos Asbestos Asbestos Mining Mining Mining AccolationAccolationAccolation Acolation --- ----- ---- -- -------- -------- - -- ------- FEES OTS 0A Fe ean toe eee Submitted by eS ee Arthuri. fv etVerale DirectDoirrectorDirector , and The Trudeau Peinlation LaboratoryLaboratory Boe . Quoboc Aspostos Dining Assoc 1st I ^ inReport - 5-7-52 ASBESTOSIS AND PULMONARY CANCER . I. INTRODUCTION An experiment has boon in progress at The Saranac laboratory for somo e 14 months in an attempt to ascertain the influence of inhaled asbestos duct oe upon the incidence of pulmonary tumors in mice The purpose of this study is to obtain oxporituental data for correlation with experience portaining to pulmonary cancer in industrial workers exposed occupationally to the inhalation inhalation inhalation of asbestos dust . Two strains of mice one susceptiblo and the other resistant to the development of pulmonary tusors have been exposed to the acbestos dust comparable numbers of control animals of each strain have been kopt in normal air but under conditions of housing and dict identical with those of the experimental amirals The experiment has been designed to reveal a possible carlier onzot of tumors in the exposed animals than in the control group as well as to determine the total incidence of tumors occurring ovor + the entire poriod of the experiment 2 It may be stated lore that the evidence eae ne ere oo 2 obtained reveals were no tondency awe we , towards an carlier onset of tumors among exposed animals in comparison with TT re wena! - control animals control Nevertheless there does ~e" ee appear to be a trend toward a oe greator incidence of tumors in the exposed arimals than in the control animals TT NN ee wets -- ae ae It is important to note however that statistical analysis of the data obtained up to the pro.ont shows that the difforeness bobeen the oxpori- montal and tho control animals are no greater than might be expected to cccur by chance alone The experiment is not completed and it likely that if the ratios of tumor incidence at the next period when animals i . of 4 . b . ae e = ) tyros tee re LES en bue, Tyce 1st 23 5-7-53 - 11 stst 1st LM! jee anReport Report Report 5-7-53 5-7-53 5-7-53 . are killed killed killed are skilar to thone for tho is now notod may finally be shot to to month intorvel tho trond which statistically statistically significant because of the larger total number of animals observed . II MATERIALS pa ee n pan m n etme dhe v A. Dusting Material Dusting Dusting Dusting Hees Material Material Material 2 ee The dust vead in this experiment was was received experiment on January 5 1951 from theJohnson Company Acbostos Ninos of Thetford Quebec and was given the Saranac LaboratLory aboratory number 136 Koray diffraction chalysis revealed that the material con- ; tains a variety of murals of which the serpontine group amounted to - 70 por cont + Though the particulate mineral sorentine sorentine and the fibrousfibrous mineral chrysobilo yield the same diffraction pattern potrographic oxuring- tion showedshowed that there there was considerably more considerably chrysotilo chrysotilo chrysotilo chrysotilo chrysotilo chrysotilo chrysotilo chrysotilo thanthanthan serpentino serpentino serpentino serpentino serpentino serpentino serpentinoserpentino serpentino serpentino serpentino serpentino present in the material Much of tho chrysotile occurred in thick unoponed bundles but it was colinated that at least 25 per cent of the chrysotilo wan prosent as individual fibers or partially penedbundles Other components componentscomponents largely particulate included a few per cent of namotito chronito brucits tals and quarts and traces of musellanoous mineralo The quarts content was about 1 to 2 per cont per Of the particulate material particulate particlos loast 85 per cent of the less wore less less less less than than than than than 5 microns microns microns microns microns microns microns microns in in size size size , Studies of sangles of ginosphoric dust taken from the animal exposure exposure room revealed with poot to the length of the chrysotile fibers that the fibers occurred in the following distributdistributionion of cizos ~ Fibers loss than 5 herons in length 1 from 5 to 10 1 11 11 t 30 to 20 = 1 H a = 11 20 to 50 11 " EO to 100 +1 t 11 32.3 % 35.3 20.7 8.3 2.7 2.7 2.7 99.87 99.87 99.87 99.87 99.87 4 e ere unimals - unimals unimals waren The accused experiment wore obtaineod btained from the Joelison Joelison Joelison Joelison Joelison MMemeorimaloriMemorial al Laboratory Laboratory Laboratory Laboratory Laboratory at at HHaar r Kartor Kartor Leire Leire That That That Juboratory carries colorion of strains of rice identified Strain A and Strain to pulmonary pulmonary pulmonary pulmonary turers turers turers usualprostico offering the observation for tumor until : mice approach incidint of pulmonary tumers that appronizates 95 y unfse similer cibomutenses cibomutenses has an incidenct of the tumors of about 60 yor cont Since ta the rcs experirent it was proposed to study the fur fur of Gustave Gustave upon the cor incidence of twior incidence incidence above the wait ra na wait wait f f oo r rthe invligation invligation invligation invligation invligation any becaues becauebecsaues any increased increased increased increased the shel rate of 95 por ocut stavld be difficult to ascor tain + en Ca of lo bed loobed loobed loobed therefore therefore therefore therefore boca ene it lower it it had had had a lower incidence of pulmonary turer boonrue and and boonrue boonrue it it off off offered certain certain certain other other other advantages advantages cong cong Ridch was reary reary reary reary which night night Of the sovable sovable sovable stains of of pulmonnay toner streio mie mie mie available available du sufficient sufficient suf icient sufficient colony of an age 4 ratio ratio emparable the pulmonery pulmonerpyulmonery pulmonery losionls osions was low nice rumitant rumitant rumitant to to the the development development development development chiron chiron chiron chiron broaubtrooauto broauto mist to pornit to pornit obtaining NICTHOLS NICTHOLSwe tle fe Dunt Link Ons Me eee ma tee Berlinda dorised ab To fiftca yous apo for the dun into it nto h th e e are are are are are expornowal expornowal exporn wal expornowal expornowal expornowal expornowal expornowal expornowal ae i study study study study study the consists ossenbelly in is doing used is doing doing used in agitatioan gitation at 0 : ; = 0 ~ Quebec Interim Interimboatos Hining Ascoc 1st Interim Report 5-7-52 of the asbestos dust by a rotating paddlo in a large hopper filled with asbestos dust to approximately the level of the axle of the paddle The edges of the paddle are equipped with stiff wire brushes to break up the small balls of asbestos which tend to form The dusting Dochanism is kept in operation inside the foot cubical dust roon for 8 hours a day 5 1/2 days per week Animals which live continuously in the room which the ee is > 7 . . toe - en wn vontilated with freshfresh cleanaiwrhen the dust disseminator is not in oport- tion Atmospheric samples are collected in the room at regular intervals ee <<" 2 with Greenburg midget impinger and dustcounts made by the light field technic with a microprojector The have dust coqut aves raged dust 77 million foot particles cubic ae te eee particles por cubic of air Te ee In generals the fibers havo total a ee Seed total represented about 6.6 per cent of the ee ee ae .. ert count or 53,8 million of tee ne er fibers about are longer the total number of , about 11 per cent are . a than than 20 microns oe, ee Tee. as It will be recalled that in regard to the production of asbestosis , the longth fibers greater than 20 micronsin one _ _. longth are ofthe greatest importance n are T ana start B. Animals At the start of the experiment260 mice wore placed in the dust room 130 of these were Strain C nico and 130 were Strain C57 black nice Of cech strain 30 more males and 100 were fonales This sex ratio was dictated by space requirements since rale mico tend to fight and must be kept in small groups while many females may safely be placed in a single coge The total number of control mice was 340 witwhith con distribution con a distribution distribution similar similar to that of the exposed groupo In order to followthe development of tumors and to observe any possible earlier onset of tumors among the exposed mico experimental were animals and control quincho quincho killkeildled at at regular intervals after the regular . o 0 . Quebec pecics Haning Assoc 1st Inturin Roport 5-7-52 beginning of the exposure to dust The intervals chosen were 2 4 6 10 and 11 months the next interval has been set for 16 months At each period 3 experimental and 3 control animals were killed of each group of 34 animals 17 more Strain C and 17 wore Strain C57 black In cech caso the 17 animals included |, males and 13 Cowles IV RESULTS Preree. The observations made at cach of the periods when animals were killedkilled periods are presented in table 1. It will be noted that the total number of animals examined in cach of the different groups varies slightly This ver^>ationver^>ation in the result of a few spontaneous deaths in each group The observations nade in such cases harte beon incorporated in the data for the next period In many instances animals dying spontaneously have toon eaten by their cagemates It has bosh necessary to discard those animals completely from tho record of experimental observations It will be noted blat within Strain G there is a higior percentage of tumors among animals exposed to asbostes dust then among the controls The samo situation is true within the Strain 057 black Obviously the same condition is true also of the overall figures combining both strains The significance of all those porcentage incidence figures has been tested statistically by means of the recopied formula S.E. oo Standard orror of difference in incidence between two groups Total number of animals in the grov^ j _ 0 ay ~ wtiwbe 425001 1st1st1 rim Report Report 5-7-52 P1 = Per cent of animals with tumor in a specific group of animals * * 8 " Qs without tumor in the same specific group Thus Q1 = 100 ) Ya F2 02 Corresponding symbols for another group of animals It will be recalled that if an observed difference in porcentage incidence between two groups is greater than twice the standard error of that difference the difference may beconsidered to be significant and due to factors other than chance If the observed difference is less than twice the standard error it is very likely due simply to chanco In table2 the observed difference in the incidence of pulmonary tumor and the critical value of twice the standard error of difference are compared for the various groups I will be noted by comparing the values that none of the observations nodo to dato tre statistically significant An example of the method of calculation follows Calculation of standard error of difference between over incidence of tumors in the control animals and in the experimental mice from data in tablo 1 : Incidonco of turiors poriental animals 9.5 Control anuals 5.5 Number of animals observed Experimental animals 379 Control animals 1.31 S.E. \r9.5 . : x 909 .5 4 5.5. a 5 94.5 > 1.79 381 2.77 4.80 2.87 4.80 4.+80 2.87 * 17.07 17.07 : CO . . ; ~ Mining sbestos Assoc 1st Lrin Report 5-7-52 Thus a difference up to 5.5 per cent is likely to occur by chance The observed difference of 1 per cent 9.5-5.5 9.5-5.5 9.5-5.5 = 4.0 may therefore have occurred simply by chance distribution of enimals enimals and is not statistically significant V. CONCENT It will be apparent from a review of table 1 that the experiment is now in its most important phase The incidence of pulmonary tumors has begun to rice for animals killedat control animals and for the animals the month period both exposed to asbestos dust for tho The incrcos incidence is moro pronounced for the group exposed to dust but the rise has occurred at about the same time in both groups The differences betwoon the two groups however as has been noted are not statistically cignifi- cant The impression is gained from an inspection of table 1 that the large numbers of animals killed ourly in the exporinent aro weighting the final figuroo and detracting from the magnitude of the incidence of tumor It must be noted that this study been an experimental one and that it has been necessary to make observations by killing animals for study at regularintervals It appears that by means of a rather rather involved statisti cal procedure the disproportionate weighting of the values by the data of the early observations can be eliminated This procedure will be carried out after final observations have been made since it is too laborious an undertaking to be performed at the present stage when the values obtainobtaeined d would be only of academic interest  Quebc sbestos Mining Assoc 1st Interim Report 5-7-52 o It may he noted in pessing that if the over incidence of tumor anong the exposed animals had been only slightly greater per cont rather than 9.5 per cent difference between the incidence for the , exposed group and for the control group would have been statistically significant An experiment concerning the influence of inhaled asbestos dust upon the incidence of pulmonary tumors in mice has been in progress for 14 months Analysis of the results of this experiment reveals that thus fab after the animals have been exposed to asbestos dust for 14 months the dust has not exerted an influence of a degree sufficient to cause statistically significant alterations in the incidence of pulmonary tumors The of final phases of this exporiment will be of great importance since at the nost rocont period when animals were killed for study the incidence of tumors had increased and the increase seems to be more pronounced among the animals exposed to asbestos dust than among the exposed control animals AJV je 280 1st 1 in Mining 09070 MinMiningin MininggMining Assoc Mining Assoc Assoc Assoc Report 5-7-52 Lf Tablo i Occurrence of Pulmonary Tutors in Mice Exposed to Asbostos Dust and in Mico Not Exposed to Dust ; Strain of nice Strain C Tuner susceptible strain of nice Period of observation mouths NJORA NJORA NJORA Mumber of nico with tumor in the groep exposed to asbestos dust Hala 0 Penalo Number exmined Incidence of tumor Strain C57 Black Tumor susceptiblo - 0 strain of mice 20 14 Number examined Incidence of turer warn. 94 129412.36 129412.36 129412.36 129412.36 129412.36 0 2 OHN 585 : : : 5.95 5.95 5.95 Number of mice with tumor in the control group not exposed to duot llala Perklo 0 1 oO oO oe Oogo 20 69 Incidence of terror in both groups | 27179 27179 27179 -9.58 10181 10181 Zy Mclignant Mclignant Mclignant Mclignant Mclignant tt . wutts ae * a. . 0 0 Table 2 . Quabsbestos Quabsbestos ining issoc 1st Inderin 5-7-52 1st Inderin Report 5-7-52 . Difference in incidence of Pulmonary Tumors in Nice Exxposed to Asbestos Dust and in Nice Not Exposed to Dust Groups of mico compared Strain C asbestos group VS Strain C control group StrainStrain C57 black asbestos asbestos group 178 Strain C57 black control group All asbestos croups both strains V3 All control groups both strains All asbestos group at 10 and 11 months VE All control groups at 10 and 14 months All asbestos troups total incidence Va ~, ad All groups total incidenco Observed difference in incidence ver cont 5.2 5.2 5.2 4.0 8.3 Trice the standard error of difference per cont 8.8 Statistical significence of observed difference aed De ae tere ed Not significant 6.5 NotNot significant 5.5 5.5 5.5 13.0 13.0 L.C Not significant 1 Not significent t The values for all groups total incidence are based upon observations for the asbestos and the control groups and also for groups coroposed to tro other dusts oxide and quartz another experiment