Document Rjm90VXBYn4jNLV1zkYB2Z6jk
AR 286 -- 0752
MICROBICS MICROTOX TOXICITY TEST
EST SUBSTANCE
sm-- s --
Identity:
A mixture containing perfluorooctanesulfonate, which may alsobe referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 519. The test sample is FC203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 31%
sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12%
sodium lauryl sulfate, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely
characterized concentrations of impurities. Data may not accurately reflect the toxicityof the fluorochemical component of the test. sample.
METHOD:
Method: Microbics Microtox Procedure
Test type: Acute static
GLP: No Year Completed: 1989
Species: Photobacterium phosphoreum
Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes `Statistical methods: EC50 values calculated by statistical linear regression
program provided for Microtox
Test organism source: Microbics' Reagent.
Test conditions:
Diluent: Microbics 2% NaCl solution
Test temperature: Not noted.
Stock and test solutionpreparation: A primary 2000 mg/L stock solution was prepared in Millipore Milli-Q" water. The pHofthe stock solution was 6.8 and an osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL stock solution. Appearanceoftest solutions was noted as "completely
miscible, no color apparent". All test solutions were made by proportional
dilutions.
[Exposure vessels: 4 mL cuvettes.
Number of replicates: two
Numberofconcentrations: Four plus a blank control
--
Element basis: Percent light loss
RESULTS Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
001116
Element value:
155--mmiinnuuteteEECCss==372600(3(62050----38207)0)mgm/gL/.L. 30-minute ECzo = 230 (220 - 240) mglL
Element values based on nominal concentrations.
RSuebmsatraknsc:eTReestmianrgkwsafsielcdo.nTduhcetveadlounesarmeipxotrutreed aasppdleystcoritbhaetdmiinxtthuereTeasntd not the fluorochemical proportion alone.
CONCLUSIONS Tdehteetremsitnseudbtsotabnec2e3300-mgm/iLn.uwtietEhCaso9f5o%r Pchoontfoibdaecncteeriinutemrvpahloosfp2h2o0retoum24w0as
mg/L.
SPauublm,itMtNe,r:5531M33Company, Environmental Laboratory, P.O. Box 33331, St.
DATA QUALITY.
RHeolwieabvielri,tys:amKpilmeispcuhrirtyanwkaisngn2o.t pTreospteirnlgymceheatrasctalelrcirzietderiaanfdorthqeuasltituydytelsatcinkgs. analytical confirmation of the amount of fluorochemical proportion in the
solution.
REFERENCES
TSth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMG1C3o5m8p-a1n,y1,98E9n.vironmental Laboratory,
THER
Last changed: 6/26/00.
001117
:
:
'
ENVIRONMENTAHL EATON! MICROBICS MICROTOX" SYSTEM
RAW DATA
Test susstance: EC=RO3CE
TEST DATE:,
=
=
ANALYST:
i
fap509 0
wa/asg-|
P34 Ps y
00
SAMPLE CUVETTE
@) Fo ss cocamanon | FE ma
= | 5-MIN. "Tou
1(5
1(15)
30-MIN. 30;
[a 23
Dp UPLIo CATEn
a5
8I
7
70
82
145"
2 | 55 | 4s H]
Duplicate |96 [59 | 49 | 45
8
A550
9A | 46 87 23
co
Duplicate
47 44 39 35"
8
S00
27 | 3&2 | as| 21
co
Duplicate
93 | 34 [26 | AR
8s
1000
94 | a4 18 | 15
es
Duplicate.
96 | 43 17 | 14
Test Substances tock Solotons Zaopy Jonol Milli-Q wetter
Yellow Ligord
fade ond =2.0
Apparently completely.miscible AoColor apparent-
Comte
AdjustmentsStAedcdkeSdona.copmh=g
6.2
Natll
vo
10m !Stock Solotion
Dilvent 5
Miccobice 29 Maell Solorion
.
Tnitial Dilvtions 2 Tniria\ Con
Couple. +he. Cinal assay Goneninatons
''
menial Se
ns were mired rtothe
TRESS lenininrgint (hm) imei| k1e5
'
PE 2oh HiLen
.
"ag
MICROTOX(r) DATA SHEET BEST COPY AVAILABLE:
LR G1358-1 FC-205CE S-MIN EXPOSURE 6/16/87 SAB
i
PAIR #
T 23
4
5. 78.
BLANK
BLANK
cose
conc,
To/1t
125.008 Ewe
215205..000000
9962..00/7 5496..00
250, 000
97.0/ 49.0
550000..000000 %1000.000
837..00/7 3324..00 54.07 24.0
Bo7/%B1t0=00.09050. /. 8196.07. 23.: 0
RATIO= 0.8526
6-088
5.368 00..378075
0.688
11..331382 + 22..353599,
-= 370 en (38537 TO38L1.99)2 . ) 370
6-EST
Fervent Lant
5.37
--T
00..377052
ayg o cl
0.702
HU el
11..331133 | 2.856
C87ETL
7
x
2.486 1" 5) T7R2
Tr
-
P oDG xI1T0E '
(30--380) mlb v pi3g'
R =0.99915 SLOPE = 1.1045 INTERCEPT = +5.9135
wil
#41 0R.E0S5.4 25 00..005325 a 0.03 S&$ 010..003355
7a 0o.o05m1
GE
.
LIST 2RWN FFCc 0 kK N
EN
PROB9.8 2 o '1 mmmmmmmmmmm mmmm mm mmm3 m mmm mm:m mm mmm mH[Et {Es
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to
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BEST COPY ym eBteLk
Abie
LR 61358-1 FC-203CE 1S-NIN EXPOSURE 6/16/89 SAB
YEABLE
PAIR # con, c. 231 211522055...050000000 as 355000..000000 . 500:000 e7 7%11000000.000000
BLANK Bos/Bt= 95 /
BLANK RATIO= 0.7: 789
oTor/1t 589682i7.0007/7 443597...000 5&77..007/ 2359.000 $5.07 2600
69.4.007/ 1187..00 74
6-085
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500.1559115993
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0T 1.19754739 893Eug RiLR
33..221100
725 Le
Sg
ID. Gobs Yipg
JEGaHmATEy Fh
EC So = 264.385 (254.982 T0 274.134) Abo ( 250-27mg0I)L.
a
R =0.99904 SLOPE = 1.1388 INTERCEPT = +5.5774
TMi
#%1 oR.E0Ss.k 23 00..005367
4 0.037
S& 00..00337
87 00..005559 b
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LR 61388-1 FC-203CE
WiGROTaX(r) DAT SHEET SeyCp. gp
3O-MIN EXPOSURE 6/16/89 SAB
Alay, 3
rama ow
gTiTg 125.m00a0Ju
552 1s2255o0..o000o000.
5 500.000
mms
88707 --41.0
5a926..r00/7os3453e..00
87.07 21.0
om
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10.1.l05057a42z
2.053
sr
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01.1.500777088
2.057
Been bk
37 oP
H23o0%o Ci
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u
%1000,000 #1000, 000
94,07 15,0 96.0/. 14,0
3.618 8.083
Bee. 3.880
Ig 8
1
BBLLAANNKK RABToI/OBSt=0.7953683/ 70
2 wiJai 0T) eGooggmynRlu
ECc 50 =: 20.637 (220.720 T0 241.001) 230 (230----340) mae REIhNr
R =0.99876 SLOPE = 1,0861 = INTERCEPT = 45.4408
"ind
wh RES. PRO:B. 2 A : rima|
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001121