Document Rjm85yv2nRDLayvNrwkR74Qzk

Ak _ 0135 ACUTE TO XIC ITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE________________________________________________ Identity: Mixture containing Perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206A. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was cc785-22. The test sample is FC-206A. Current information indicates it is a mixture of 0.92% PFOS, 18.75% diethylene glycol butyl ether, 75.44% water, 1.83% hydroxy foamer, 1.75% sodium octyl sulfate, 1.2% poloxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazde. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:_____________________________________ ___________________ M ethod: U.S. EPA 1975 Type: Acute static GLP: No Y ear com pleted: 1978 Species: Lepomis macrochirus Supplier: Dale Fatting Fish Farm, Brady, NE. A nalytical m onitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical m ethods: LCso values and 95% confidence intervals were calculated using the U.S. EPA (Duluth) probit computer program. Test fish age: Juveniles Length and w eight: Average length = 45 mm Average weight = 0.92 g (wet) Loading: 0.8 g/L Pretreatm ent: None Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Hardness: 235 mg/L as CaC0 3 A lkalinity: 221 mg/L as CaC0 3 C onductivity: 418pm hos/cm Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a 30------minute transition period was maintained throughout the testing period. Stock and te st solution preparation: The test material was added directly to the dilution water on a weight / volume basis. Concentrations dosing rate: Once. 001591 Stability of the test chemical solutions: Not noted. Exposure vessels: Glass aquaria (35x30x20 cm) containing 16 L of test solution. Number of replicates: 2 Number of fish per replicate: 10 Number of concentrations: live plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.9 - 8.0 (control exposure) 7.5 - 7.9 (1350 mg/L exposure)* Temperature range (0-96 hours): 17 -1 8 C Dissolved oxygen range (0-96 hours): 5.8 - 9.9 mg/L (control exposure) 0.1 - 7.3 m g /l (1350 mg/L exposure)* * 1350 mg/L (intermediate concentration) exposure values used because the highest three concentrations used resulted in total mortality by 48 hours. Remarks: Very low dissolved oxygen concentrations ( 0 .1 -1 .8 mg/L) observed in the exposure concentrations (except for the control) by 48 hours. RESULTS________________________________________________________ Nominal concentrations: Blank control, 10 00,135 0,18 00, 2400, and 3200 mg/L (listed in text as g/L) Element values: 96-hour LC50 = 1300 (1100 -1 4 0 0 ) mg/L (Duplicate 1) 1200 (1000 -1 3 0 0 ) mg/L (Duplicate 2) Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test sample 96-hour LC50 range for bluegill sunfish was determined to be 1 2 0 0 -1 3 0 0 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks 001592 analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ______________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1978. O T H E R ___________________________________________________________ Last changed: 6/26/00 359 TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN i Important - I f report is printed on both sides o f paper, send two copies to TCC.) D a ta 11/2/78 O ivjsion Environmental Laboratory (EE & PC) P ro je c t Report T itle Commercial Chemicals FC-206A cc 785-22: Results of Aquatic To D . L . Bacon A u th o r(s ) A. N. Welter N otebook Reference 96-Hr. Dopt. Num ber 0535 P roject N um ber 9970012600 R eport N um ber LC(-n Test: > 003 E m ploye N um ber(s) 09362 No. o f Paget In clu ding Coversheet 6 SECURITY ^ & Open Closed 3M CHEM ICAL ^ REGISTRY * New Chemicals Reported Yes H No KEYWORDS: (Select terms from 3M Thesaurus. Suggest other applicable terms.) EE & PC-DV. (Environmental Lab) Toxicity 'Aquatic) CURRENT OBJECTIVE: To determine the acute LC value for the bluegill sunfisfc (Lepomis maorochirus) exposed to varying concentrations of FC-206A cc 785-22. REPO RT ABSTRACT: (20 0 -2 5 0 words) This abstract inform ation is distributed by the Technical Communications Cam alert 3M `ers to Company R & O . The acute static 96-hr. LC for bluegill sunfish (Lepomit maaroahirus) exposed to varying concentrations of FC-206A cc 785-22 ranged from 1.2 to 1.3 g/1. Information Liaison Initials: J 001594 FC-206A 2- November 2, 1978 CONCLUSIONS In a replicated static 96-hour LCj-q aquatic test, the calculated LC50 values for bluegill sunfish ?Lepomis maoroohirus) were 1.2 andu1.3g/l respectively. Materials possessing an LC5Q of this order of magnitude are considered to present an insignificant hazard based on the NIOSH aquatic toxicity criteria. INTRODUCTION The test substance FC-306A cc 785-22 is an amber liquid. Tests performed on this material evaluated its aquatic toxicity and the data generated form the basis of this report. METHODS A. Aquatic Testing The test protocol utilized for this study was modeled after that described by US/EPA 1975. B. Acclimation Procedure The bluegill sunfish (Lepomis maoroohirus) were obtained from a private hatchery.* Stock fish were held in a fiberglass holding tank filled with carbon-filtered well water at 14-15 C. A daily photoperiod of 16 hours light and 8 hours dark with a 30-miaute transition period was maintained throughout the acclimation and testing period. The fish were fed "Tetra-Min"# daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated for 15 days prior to testing. An acute short-term (96-hour) static bioassay was performed on FC-206A. Carbon-filtered well water of known composition was used as the diluent (Attachment 1). All glass aquaria (35 x 30 x 20 cm) containing 16:1 of diluent or diluent plus toxicant comprised a study chamber. Ten blugills, uniform in size, were tested at each chemical concentration. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. Bluegill experiments were conducted at 17 C. Mortality, temperature, dissolved oxygen level and pH of all test solutions were recorded. General observations relative to behavioral changes were similarly recorded when appropriate. These studies were performed in replicate using the following FC-206A (cc 785-22) concentrations: 1.0, 1.35, 1.8, 2.4,"and 3,2g/l. LC50 values with 95% confidence limits were calculated using the Us/EPA (Duluth) probit computer program. Organisms used in this study were considered to be generally healthy and free of disease. *Dale Fattig Fish Farm, Brady, Nebraska. #Commercial Fish food of known composition. 00159! FC-206A -3- November 10, 1978 RESULTS The bluegill sunfish (Lepomis maaroahirus) used in this study had an average weight of .92+.36 grams and a length of 4.5+0.6 cm. The loading factor for these~studies was 0.6 g/1 below the~maximum recommended loading factor of 0.8 g/1 (1). Temperature and pH ranges throughout this study were 17-18 C. and 7.6-8.0 pH units respectively. Dissolved oxygen values decreased sharply 48-hour post initiation of this study. Test aquaria solutions originally were clear (normal DO levels), became straw colored (low DO), and at termina tion of this study had assumed a grayish-white color. This latter color change was accompanied by a relative increase in the dissolved oxygen content. Although the DO levels determined were in the range of 0.1-0.3 mg/1, there was no apparent increase in mortality due to what would be assumed to be a general hypoxic situation. The absence of an increased mortality attributable to low dissolved oxygen content relationship is taken as presumptive evidence that the calculated LC_0 values are reflections of test chemical induced mortality, per se. In this replicated study, the 96-hour LC5Q values were 1.2, range 1.0-1.3 and 0.3, range 1.1-1.5 g/1 respectively. These values translate to LC5Q values of 1200 and 1300 ppm. Chemicals possessing an LC50 value ifiuthis range are considered to be an insignificant hazarato the aquatic environment (2). REFERENCES (1) US/EPA - Methods for Acute Toxicity Test with Fish, Macro invertebrates and Amphibians, Ecological Research Series 660/3-75-009, 1975. (2) Registry of Toxic Effects of Chemical Substances, '76 Edition, Christensen, H. E., and E. J. Fairchild, Eds. p. XV and C i , U. S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, DC, June, 1976. "1 i'Ll'-ILx ANW/cen Attachments 001596 _-- --"Tffn x -- - i --- l AlB. gE^ur.CT mq. H ai farAmMUe^ POTI r- It" 313 FOUM A H P L t )&$RlfTl*lJ : '0*v Q / u. %ltT TTACHMENT *1 ^ . . t . q n ia m 7 -/fi-ffi m a i /i tU lr n f* <1 P --J * 1 7 -a r-M 7*7 <| VVO * 1 0 p __ *77 *fe <*.| 0 *77 I U <* 7 - 3 / - ? f ________ ; 7t7 f-W -7 7t7 7*4 7 -* '-7 * A 7-'7ir 7#7 - - 7*7 <0 ./ | *1 <| <| / yi* Yii i t i 1B* I*T1 ^ H fe l J 1 222 4 m 17* 9 * 1 1 jMl 0 B E ItL <M 9 r c i L s : ^ <1 .! 4 1 1 4 i<.) 0 In a lo .!! l-a a -7 # 7*7 <0.1 4 m m fi -a i- 7>S <0'l 41' Hor 2 X0 - C> 1 1 3 331 >*/ icIr TaiCc Oo Tr rvnUDrTV taA\V/MaUiilMaDdL Xe- " .... "1 .-1 -- | i ..... -- ,- -* ~o d .ia a i 7 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHEET 'type Test 3 i<U iC 74 h. LCyr> Material Tested f -jC'fc A Lot C .72 'rest Organism 7 / ^ i ! h V e n d o r D a t e R e c'd y / ^ / ; y Batch No _r -J rM X Avg. Wt. . ^ x , 3 .f*i grams Avg. Size ^ )-S - S i _____ cm. Test Solution Volume / C._____ Liters/Tank No. Organisms per dose [ o ___________ /L"vc Time S tart e d j ^ ^ fi Date Started l o / z o / r t i Date Completed j o ) i Hl '7'S t?W r J Dilution Water Carbon f i l t e r e d well (Temp. ' / C . , D.O. y. y ppm, ptt ? 5" ) water In vestigator(J^&it<Mw<J^>(// 7 / ^ LC 50 (95% Confidence Limits) / 3 /. *f (Full Signature!) 001598 "FnV )KNMIInTL NCINLIIULNG -LABORATORY AQUATIC TOXICITY DATA SHEET T/pe Test fctfcc '-ic A '-Cc,-______ Material 'Tested / C Q cL /} /in ? Lot a 7$n- Z l- Test Organism ?Jct^uuXii_____ Vendor , Date Rec 'd V,^v76 Batch No 7 t y / y Avg. Wt. . Q/ J~, 3VQ grams Avg. S ize *y. ,)" - S i _____ cm. Test Solution Volume IL ______ Liters/Tank No. Organisms per dose 0__________ tmv Time Started /ft Date Started jo/f g/;$ Date Completed j O H i h i /rjuw Dilution Water Carbon f i l t e r e d well (Temp. Jjf_ C , D.O. 7, 7 ppm, pit 7. j- . water f Investigator (Full Signature! 4u U / J n \ LC 50 (95% Confidence Limits) \.L ,.3 Cone. e>Jg/l. Total \mount Added Control / i Lk. JJL M A 5V j?'/-Z ?4-Hours Total D.O. No. Dead . j L ?/ 11 Vi 7.3 0 2a iii IX u 21 (,.3 1 Test Temp. C Signature Date__ 1 Comments U Q <LJr$fr\. .6 , I 1 / o / u / 7* 48-Hours Total No.Dead pH 72-Hours D.O. Total D.O. PE.?.. No. Dead H PPtn 96-Hours Total D No.Dead w . 1 O &0 XL 0 Ho fe.O O #0 -3=JL c2 7-1 ,2 2^ .2. ZO. 2cZ_ O k jo Zk. jo ~b> CfcMi\ti*? 7^ rU j& U j 10/ilf?# xJ jcjhJ .a i o' ithjlilAwife, /o<Mii .Aa CjO^U a ^ M > J i.a-------------------------- ~aq-jl Effects other than Mortality Cone. mg/1 Symptoms or Reactions Control . (>3S /. 2X 5X i"nature Date , 4 Comments: 24-Hr. TCfTo Total No. Affected 48-Hr. 72-Hr. 0 O 1 A jo (0 riiiuUH tal in O S 9 rs 0 I a X /?>N 0 * -- -- --i 96-IIr. <b T? a. -- --- BEST C O P Y AVILABLE- 001599