Document RjGb0yvQX2XrL0XLExazBZLB
MICROTOX@ TOXICITY TEST
TEST SUBSTANCE
Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateF,C116,FC-126,FC-169,orFC-143. (Octanoicacid, pentadecafluoroa-m,monium saltC,AS # 3825-26-1)
Remarks: 3M productionlotnumber 390. The testsample isFC-126,a whitepowdery solid.It'psuritywas not sufficientclhyaracterizedt,hough currentinformationindicatesitisa mixtureof78 -93% testsubstance and 7 -22% C5, C6, and C7 perfluoroanalogue compounds.
METHOD:
Method: Beckman's Microtox@ "BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1987 Species: Photobacteriumphosphoreum Analyticalmonitoring:Lightloss. Exposure period:30-minutes Statisticamlethods: Not noted. Test organism source: Not noted. Test conditions:
Diluent:2% NaCl Microbic'Rseagent Deionizedwater pH: 6.5 Stock and testsolutionpreparation:A 2000 mg/L stocksolutiownas prepared by dissolvin2g00 mg ofthe testsolutionin100 mL diluent.Test solutionswere prepared usingaliquotsofthestocksolution. Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: Cuvettes. Number of replicates:two Number of concentrations:Four plusa blankcontrol
RESULTS
Nominal Element
concentrations:Blank control4,20, 560, 750, and value: 5-minute EC20 = 810 mg/L
5-minute EC5o = >1 000 mg/L 5-minute EC80 = >1 000 mg/L 15-minute EC20 = 520 mg/L 15-minute EC50 = >1000 mg/L 15-minute EC80 = >1000 mg/L
1000
mg/L
30-minutEeC20 = 420 mg/L 30-minuteEC5o = 870 (810-930)mg/L 30-minute EC8o = >1000 mg/L
Allelement valuesbased on nominal concentrations.
CONCLUSIONS
The FC-1 26 30-minute EC5o forPhotobacteriumphosphoreum was determined tobe 870 mg/L witha 95% Confidence Intervaolf810 to930 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking2. Testingmeets allcriterifaorquality testingb,utlacksanalyticaclonfirmatioonftestsubstance concentrations. There isa lackofcharacterizatioonfthetestsample.
REFERENCES
This study was conducted by the 3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number E1282.
OTHER
Last changed: 5/24/00
LR
ENVIRONMENTAL LABORATORY
PA
BECKMAN"S MICROTOX R SYSTEM
micROTOX R RAW DATA
TYM SUBSTANCE: TEST DATE: ANALYST:
Ec-L,A2
9 -7
to4 --3A 1)
SAMPLE CuVrrjrE
BI ci
FINAL ASSAY CONCENTRATION
rv%
L
BLANK CONTROL
DUPLICATE
INITrAU---
FINAL READINGS
READING
5-MIN.
15-MIN. 30-MIN
1(0)
1(5)
1(15) 1(30)
Cf 0
@g 1
1;7
.92
Is(C
ell
B2
YAO,
q3
7
t,I
C2
t@ u p It'ca+
7q
IB3
C3
u p ll'C&-ve-
7
71
71
B4
760
C4
u
lot
9 C) lo7
IB5
L4
39
C5
38
Tt-S4 twilite-
p)i= S-7
eo mt coi-ylrle4e(y
A,13us.4e4 -+o to-7
tui+@,
k"'CI4
bt,koe-n4
rows
409 VOW ikC
ig 4c),
'The-
dilav'isisaatys
a vxJL
DVICL -tIviel-oci-,
BECKMANOS mjCltoTOXR SYSTEM R
MICROTOX DATA REDUCTION
BLAW ITATIOS*
Final Initial
ago
BLANK C'UUVEVTETNEE BI ci
*The blank ratios
5-MIN. ATTN DIFFEREMN
DTFFERPNCF
OL190z,-0-q 7 L)-L)I
for BI
and
Cl
sho ulCdltgIreewti
t
r,-tA77 iffi )gng
withifi--0.0;2@.--
MEAN BLANK RATIOS
BI BK RATIO + Cl BK RATIO 2
LR
Date_A_:_@q -179@ Analyst
30-MIN. AMIC RITTN I nTVVV--..
z-0 -ciL71 C)CI
15-MTN-
q 9.
in-MTN-
gi@,7
NORMALIZED LIGHT LOSS (PEFFECT)
Mean Blank Ratio x Initial Sample Reading Fina-ISam-ple Reading
EAMPLE CUVETFE-rest Concentration
9,o Bi 3 B4 C4 7-6 :85 @5 /C)oo 1
5-MIN. f, o.iol
o-osi
0r,.iili o. I q 6
-AC4 'tis-
15-MIN. FT
0 -AO-3
0.1197
o - A6;k c ,A L q 0 C) c@ q
30-MIN.
0.303-
0.
0. -r,. o.(094 o &.3
% LIGHT LOSS (2,&)
x 100
SAMPLE CUV
Test Concentration
S2
.,2 149c>
hi
33
3 5& C)
14 -75C>
z_-
--
35
1.5 c> C>C)
EFFECTIVE CONCENTRATIONS
5-mlN. Z4& If
i r4 16 17
(EC's)
5-MIN.
LC 20 ic)z sic) 50
17 0
'80 li,@0- 19 190)
3 9 @0
15-MIN. 241@17
30-MIN. Z A
-13 3
A .9 Iz 9
(Calculated using "11,C"data nairs.)
15-MIN.
15- M@Al
6ac)
@7 C 5b
00
(1050 - 11,50)
L--cPo = @s 30
30-MIN.
870 (BID-q3o)
> )ooc>