Document Rj5qg8mmJOKkp0VL3Z3o4XNKk

$nduUtfal B I O T E S T Jab&uti&tieS; 9ttc. REPORT TO MONSANTO COMPANY TOXICITY, REPRODUCTION AND RESIDUE STUDY ON AROCLOR 1242, LOT #AK-255 . AROCLOR 1254, LOT #AK-38 ' AROCLOR 1260, LOT ffAIC-3 IN WHITE LEGHORN CHICKENS JUNE 4, 1970 IBT NO. J7300 1, Introduction At the request of Monsanto Company, a toxicity, reproduction and residue study on Aroclor 1242, Lot #AK~255, Aroclor 1254, Lot #AK-38 and Aroclor 1260, Lot #AK-3 in white Leghorn chickens was initiated. This report presents the results of the investigation conducted at Wedge's Creek Research Farm Division, Industrial BIO-TEST Laboratories, Inc, 0207075 TOXSTUDIES0864 UndniUtol B 1 O - T E S T 9nc. Z II. Summary ' White Leghorn chickens were fed Aroclor 1242, Aroclor 1254 or Aroclor 1260 at 1, 10 or 100 ppm in the diet. Effects on the chickens as well as on egg production, hatchability of the eggs and physical characteristics of the eggs were determined. These effects were as follows: . A. Body Weight The body weights of males fed Aroclor 1242 at 10 or 100 ppm and the body weights of males and females fed Aroclor 1254 at 100 ppm were slightly lower than the weights of control chickens. B. Food Consumption The chickens fed Aroclor 1242 at 100 ppm or Aroclor 1254 at 100 ppm ate less than those fed the control diet. C. Mortality and Reactions About the same number of birds died in all groups, including controls. These animals exhibited anorexia and became progressively weaker, until death. D. Pathology The birds which died showed evidence of malnutrition and extensive growths on the kidneys, gonads, liver or heart. Most of the birds which were sacrificed at the end of the study did not show abnor mal gross changes; however some of those fed Aroclor 1254 at 100 ppm showed changes in the heart, kidneys and ovaries. 0207076 TOXSTUDIES0865 9nduiUial B I O T E S T Xa&MatMiei, 9nc. 3 E. Egg Production Egg production in hens fed Aroclor 1242 at 100 ppm or Aroclor 1254 at 100 ppm was much lower than the egg production of the control group. F HatchabUity of Eggs There was poor hatchability of eggs from hens fed Aroclor 1242 at 10 ppm. None of the eggs hatched from hens fed Aroclor 1242 at 100 ppm or Aroclor 1254 at 100 ppm. Eggs from hens fed Aroclor 1242 at 10 or 100 ppm, that did not hatch had a large number of em bryos in the 1 to 3 cm size. G. Physical Characteristics of Eggs There were no significant differences in specific gravity among eggs from treated or from control hens. The egg shells, however, from hens fed Aroclor 1242 at 10 or 100 ppm or fed Aroclor 1254 at 100 ppm were not as thick as the shells of eggs from hens fed the con trol diet. H. Conclusions Therefore the ingestion of Aroclor 1242 at 1 ppm, Aroclor 1254 at 1 ^or 10^ppm or Aroclor 1260 at 1, 10 or 100 ppm caused no apparent effects on the mature chickens or on their eggs, including hatchability and physical characteristics of the eggs. However, the TOXSTUDIES0866 $ndiiifoial B I O - T E S T Jahi'uik'Uei, 9kc. 4 chickens fed Aroclor 1242 at 10 or 100 ppm or Aroclor 1254 at 100 ppm did not produce as many eggs as the chickens fed the control diet. The eggs from these three groups of chickens had thin shells and did not hatch well after incubation. Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC. Report prepared by: James L. Stephens, B.S. Technical Supervisor Wedge's Creek Research Farm Report approved by: Donald H. Jenkins, D. V.M. Manager and Technical Director Wedge's Creek Research Farm June 4, 1970 al:nk Manager, Toxicology Otis E. Fancher, Ph. D. Director ozoi078 TOXSTUDIES0867 PtidaiUial B IO * T E S T Xabyiaiwivi, 9*ic, 5 HI. Procedure A. Experimental Animals The animals employed in this study were white Leghorn chickens. A total of 200 hens and 40 roosters were used. B, Organization of Groups The animals were divided into ten equal groups; one Control Group (C) and nine Test Groups (TI through TIX). A structural outline of the experiment is presented in Table I. 0207079 TOXSTUDIES0868 B I O T E S T jEah&idt&ile&, Ht 0207080 TABLE I TEST MATERIAL: Three Aroclors Outline of Experiment White Leghorn Chickens Group Number of Animals on Test Males Females Test Material Control (C) -4 . 20 None Test I (TI) 4 20 Aroclbrl242, Lpt_#AK-255 Test II (Til) 4 20 Aroclor 1242, Lot #AK-255 Test IK (Till) 4 20 Aroclor 1242, Lot #AK-255 Test TV (TIV) 4 20 Aroclor 1254, Lot #AK-38 Test V (TV) ' 4 20 Aroclor 1254, Lot #AK-33 Test VI (TVI) 4 20 Aroclor 1254, Lot ffAK-38 Test VH (TVH) 4 20 Aroclor 1260, Lot #AK-3 Test vm (TVni) 4 20 Aroclor 1260, Lot #AK-3 Test IX (IX) 4 20 Aroclor 1260, Lot #AK-3 * Purina Flock Chow, Ralston Purina Co. , St. Louis, Missouri. Dose Levels in Feed* (ppm) - 1 10 100 1 10 100 1 10 100 TOXSTUDIES0869 Pwfttsl'tial B 1 O - T E S T ab&\atfr'uei, 9tic. 7 C* Dosage and Feeding All groups received the same base feed*. Test diets were prepared weekly. The test materials were diluted with acetone before addition to the feed to assure even distribution throughout the ration. An equal amount of acetone, without test material, was added to the control feed and the feed was then blended in the same manner as the test feeds. All animals were allowed water and their respective control pr test feed ad libitum. Dosing began when the birds were 12 weeks of age and continued until hatching results were available. D, Observations Observations were made daily to ascertain the presence or absence of clinical signs of toxicity indicative of test material effect. All chickens were individually weighed and the group feed consumption determined weekly, . As each group began laying, the eggs were collected daily and the egg weights recorded. When the egg weights were consistently between 50 and 60 grams, and at least 50 percent of the hens in the group were laying, egg collection for that group was started. The time required to meet these criteria was recorded for each group. 0207061 * Purina Flock Chow, Ralston Purina Co., St. Louis, Missouri. ~ TOXSTUDIES0870 $nd*iibUal B 10 - T E S T labywivilei, $nc. The egg production was collected for the following studies: 1. Egg Production and Quality Eggs were collected, weighed and candled for a number of seven-day periods. The total number and individual weights of eggs collected, the total number of uncracked and unblemished eggs and the total number of each type of defective egg were recorded. 2. Hatchability : One-half of all non-defective eggs were randomly selected for hatching. These eggs were turned twice daily during the seven-day , collection period, then placed in an incubator maintained at 99 to 100 F, with a wet bulb reading of 85 F, This procedure was followed until a minimum of 100 eggs from the control group and each no-effect test group had been .set for hatching. The following was recorded: a. Number hatched b. Number unhatched c. Viability of chicks over a' 30-day period during which feed* and water were permitted ad libitum. d. Examination of unhatched eggs for fertility and stage of development. ' e. Weight of chicks after Day 1 and Day 30. f. Gross pathological examination of chicks at sacrifice after the 30-day observation period. ozC08* * Purina Startina, Ralston Purina Company, St. Louis, Missouri. TOXSTUDIES0871 huiuiUkil B I O - T E $ T Jtabo-'urt&'Uvi, $nc. 9 3. Physical and Chemical Characteristics of Eggs One-fourth of the non-defective eggs and all of the defective eggs were examined to determine the following: a. Specific gravity (intact eggs only) b. Thickness of shells at equatorial circumference (three measurements/egg). . 4. Residue Studies The remaining one-fourth of the non-defective eggs were quick-frozen and shipped to Monsanto Company for residue analysis. After hatching results were available, one-half of the chickens in each group were sacrificed, defeathered and subjected to a gross pathological examination. Two sets of duplicate group composite samples of muscle, liver, fat and kidney were collected, one set from all of the hens and one set from all of the roosters sacrificed. One-half of the tissue samples were quick-frozen and shipped to Monsanto Company for analysis. The duplicate tissue samples and the carcasses were quick- frozen and stored until the results of the analyses were available. The remaining chickens were fed the base diet without test material for 30 days. The eggs were collected, weighed and candled during the recovery period. A minimum of ten eggs per group per week were quick-frozen and shipped to Monsanto Company for analysis. 02070B3 TOXSTUDIES0872 DnJulfolal B I O - T E S T JahwativUel, 9m. 10 IV. Results A. Parent Generation 1. body Weight Data The average male and female body weights during the test diet period are shown in Tables II through XI. The average male and female body weights during the recovery diet period are shown in Table XII. These data are summarized in Table XIIA. The body weights of males fed Aroclor 1242 at 10 or 100 ppm and the body weights of males and females fed Aroclor 1254 at 100 ppm were slightly lower than the weights of control chickens. 0207084 TOXSTUDIES0873 9nJ*tihial B IO -TE S T 9m. Sex M F M F Sex M F Week: 0 1.27 0. 95 1 1,41 0. 99 TABLE H TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Control Group Mean Body Weights in Kilograms 2 345 6 1.53 1. 04 1.64 1.18 1.6? 1.22 1.70 1.29 1.71 1.35 7 1.68 1.39 Week: 9 1.83 1.47 10 1. 89 1. 47 Mean Body Weights in Kilograms 11 12 13 14 15 1.95 1.97 1.99 2.01 2. 06 1.50 1.53 1.52 1.52 1.60 . 16 2. 09 1.61 Week: 18 2.14 1.59 19 2.14 1. 57 Mean Body Weights in Kilograms 20 21 22 23 24 2. 24 2. 27 2. 31 2. 36 2. 38 1.75 1. 75 1.75 1.78 1.81 25 2.35 1. 84 8 1.85 1.41 17 2.11 1. 57 26 2,43 1. 89 0207085 TOXSTUDIES0874 v i s a i - o i a i m ^ f uc Sex M F Week: 0 1.27 0. 95 Sex M F Week: 9 1. 85 1.45 Sex M F Week; 18 2. 20 1.64 . TABLE in TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens .Aroclor 1242 - 1 ppm 1 1.46 Mean Body Weights in Kilograms 2 345 6 1. 57 1.65 1.68 1.72 1. 80 . 1. 00 1.07 1.18 1.25 1.30 1.39 7 1.82 1.45 10 1. 95 1.49 Mean Body Weights in Kilograms 11 12 13 14 15 2. 01 2. 06 2. 08 2.14 2.17 1.55 1.57 1.57 1.58 1.59 16 2.19 1.65 19 2.23 1.66 Mean Body Weights in Kilograms 20 21 22 23 24 2.50 2.53 2. 59 2. 61 2.60 1.81 1.76 1,81 1.86 1.86 25 2.61 1.83 8 1.83 1.47 17 2. 22 1.67 26 2.68 1.88 cv TOXSTUDIES0875 B I O - T E ST Ja&Mai&ti&i, Jnc. 0207087 Sex M F Week: 0 1.28 0. 95 1 1.45 1. 00 TABLE IV TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1242 - 10 ppm Mean. Body Weights in Kilograms 2 345 6 1.41 1.49 1.52 1.55 1.59 1. 05 1. 17 1.22 1.29 1.38 7 1.66 1.41 8 1. 63 1.48 Sex M F Week; 9 1.60 1.41 10 1. 71 I. 52 Mean Body Weights in Kilograms 11 12 13 14 15 1. 71 1. 77 1.80 1.81 1.82 1.50 1. 53 1.57 1.62 1.63 16 1. 84 1.63 17 1. 86 1.63 Sex M F Week: 18 1.89 1.59 19 1. 86 1. 59 Mean Body Weights in Kilograms 20 21 22 23 24 2. 00 2. 03 2.05 2.11 2. 11 1.74 1.79 1.82 1.90 1.82 25 2. 08 1.84 26 2. 11 1.82 TOXSTUDIES0876 In A o h h l B IO -T E S T !< Sex M F Sex M F Sex M F Week: 0 1.28 0.96 1 1.41 1. 02 TABLE V TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1242 - 100 ppm Mean Body Weights in Kilograms 234 5 6 7 1.52 1. 58 1.61 1.67 1.69 1.70 1. 07 1.15 1.19 1. 27 1. 37 1. 38 Week: 9 1.60 1.46 10 1.75 1. 52 Mean 3ody Weights in Kilograms n 12 13 14 15 1.78 1.76 1. 75 1.77 1. 81 1.47 1.52 1.47 1.65 1. 67 16 1. 79 1.66 Week: 18 1.78 1.63 19 1. 74 1. 62 Mean Body Weights in Kilograms 20 21 22 1.79 1.92 2. 05 1.66 1.69 1. 88 8 1.72 1.44 . 17 1.78 1.63 890*020 TOXSTUDIES0877 laJuihial B IO -TE S T A. Sex M F Week: 0 1,28 0. 96 Sex M F Week; 9 1.83 1.51 Sex M F Week: 19 1.85 1.66 1 1,40 1. 00 TABLE VI TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1254 - 1 ppm Mean Body Weights in Kilograms 2 34 5 6 1.46 1, 55 1.58 1,61 1.64 1. 06 1. 15 1. 20 1. 27 1.40 10 1.86 1. 54 Mean Body Weights in Kilograms 11 12 13 14 15 1. 91 1. 99 2. 01 1. 96 1.98 1. 54 1.61 1. 62 1.62 1. 65 20 2. 22 1. 69 Mean Body Weights in Kilograms 21 22 23 24 25 2.21 2.24 2. 22 2. 26 2. 26 1.73 1.73 1. 71 1.74 1. 93 7 1. 76 1.42 8 1.80 -1.49 16 1. 99 1. 66 17 2. 00 1.69 6904020 TOXSTUDIES0878 B IO -TE S T JalmotMiGi, Sex M F Week: 0 1.2$ -o. 96 Sex M F Week: 9 1.73 1.46 Sex M F Week: 18 2.06 1.64 1 1.40 0. 96 TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1254 - 10 ppm Mean Body Weights in Kilograms 234 56 1.49 1.47 1.46 1.60 1.68 1.10 1. 14 1.21 1. 32 1.40 10 1.72 1. 52 Mean Body Weights in Kilograms 11 12 13 14 15 1. 80 1.88 1. 93 2. 00 2. 03 1.50 1.52 1. 55 1.66 1.63 19 2. 03 1.64 Mean Body Weights in Kilograms 20 21 22 23 24 2. 07 2. 08 2. 07 2. 00 2. 14 1. 62 1.66 1. 68 1.61 1. 63 7 1. 73 1. 44 16 2. 01 1.60 25 2. 07 1. 54 8 1-75 1.49 17 2.03 1.60 26 2. 30 1.84 O6OZ0ZO TOXSTUDIES0879 Dndnibtiol B IO -T E S T <. Sex M F Week: 0 1 .'28, 0. 95 1 1.44 0. 98 TABLE VIH TEST MATERIAL: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1254 - 100 ppm Mean Body Weights in Kilograms 2 34 56 1. 54 1.62 1.67 1.70 1.73 1.05 1.12 1.17 1.22 1.27 Sex M F Week: 9 10 1.77 ' 1.73 1.42 1.46 Mean Body Weights in Kilograms 11 12 13 14 15 1.62 1.85 1.85 1.81 1.84 1.49 1. 54 1.52 1.51 1.50 Sex M F Week: 18 1.56 1.48 19 1.70 1. 55 Mean Body Weights in Kilograms 20 21 22 1.75 1.78 1. 83 1. 50 1.54 1.81 7 1. 77 1. 37 16 1.74 1.41 8 1.82 1.40 17 1.70 2.47 160/ 020- TOXSTUDIES0880 9 n d ik ia l B I O * T E 5 T Jahyiato-'ii& i, Hue, 00 Sex M F Sex M F Week: 0 1.28 0. 96 Week: 9 1.86 1.44 TABLE IX TEST MATERIAL: Three Aroclors Test Diet Period Body "Weight Data White Leghorn, Chickens Aroclor 1260 - 1 ppm Mean Body Weights in Kilograms 1 2 34 56 1.48 1.55 I. 65 1.66 1.74 1.88 . 1. 00 1. 07 1.16 1. 22 1. 30 7 1.81 1. 39 10 1.91 1.46 Mean Body Weights in Kilograms 11 12 13 14 15 1.92 2. 02 2. 06 2.11 2.10 1.49 1.55 1. 57 . 1.59 1.61 16 2. 10 1.59 8 1.85 1.44 17 2.18 1.61 Sex M F Week; 18 2.20 1.59 19 2.19 1.65 20 2.24 1.66 21 2.23 1.69 22 2. 24 1.69 23 2.13 1.69 24 1.79 1,79 25 1. 76 1. 76 26 1.84 1.84 ' TOXSTUDIES0881 B IO - T EST M jl&iai&Uei, 9*tc. Sex M F Sex M F Sex M F Week: 0 1.28 0. 95 Week: 9 1.76 1.47 Week: 18 2. 08 2. 08 1 1.47 0. 99 TEST MATERIALS: Three Aroclors Test Diet Period Body Weight Data White Leghorn Chickens Aroclor 1260 - 10 ppm Mean Body Weights in Kilograms 2345 1. 54 1.62 1.68 1.72 1. 05 1.17 1. 24 1.31 6 1.76 1. 39 10 1.80 1.48 Mean Body Weights in Kilograms 11 12 13 14 15 1.89 1.93 1. 98 2. 03 2. 05 1.55 1.59 1. 61 1.63 1.63 19 2. 12 1. 69 Mean Body Weights in Kilograms 20 21 22 23 24 2.13 2.12 2. 19 2. 22 2. 22 1. 68 1.69 1.73 1.75 1.72 7 1.79 1.41 8 1.84 1.51 16 2. 02 1. 62 17 2. 07 1.65 25 2. .18! 1.71 26 2.52 1.92 tbO iozo TOXSTUDIES0882 VU( tatw jm a/cf 1 s 3 1 - O I 8 Sex M F Week; 0 1.28 0. 95 Sex M F Week: 9 1.77 1.48 Sex M F Week; 18 2. 00 1.64 1 1.45 1. 00 TABLE XI ' TEST MATERIAL:Three Aroclors Test Diet Period Body Weight Data White Leghorn. Chickens Aroclor 1260 - 100 ppm Mean Body W eights in Kilograms 234 5 1.46 1.55 1. 59 1.61 1. 03 1.14 1.21 1.27 6 1.70. 1.35 7 1.77 1. 43 10 1.80 1.51 Mean Body Weights in Kilograms 11 12 13 14 1.82 1.69 1. 94 1.93 1.53 1. 55 1. 56 1.57 15 1.97 1. 58 16 1. 97 1. 61 19 1. 90 1.67 Mean Body Weights in Kilograms 20 21 22 23 24 1. 97 1.92 1. 99 I. 99 2. 00 1. 63 1.63 1.68 1. 71 1.68 25 2. 05 1. 36 8 1.73 1.46 17 1. 98 1.64 TOXSTUDIES0883 DiuHnifatal B I O - T E 5 T ^a^fwityuei, 9nc, 21 TABLE XII TEST MATERIAL: Three Aroclors Recovery Diet Period Body Weight Data White Leghorn Chickens Group Sex Week: Mean Body Weights in Kilograms 26 27 28 29 Sacrifice Control M F 2.43 1.89 2.42 1.89 2.42 1.85 2.45 1.85 . 2.44 1.88 Group Sex Week: Mean Body Weights in Kilograms 26 27 28 29 Sacrifice Aroclor 1242 M 1 ppm F 2.68 1.88 2. 68 1.89 2,71 1.92 2.70 1. 90 2.70 1.86 Group Sex Week: Aroclor 1242 M 10 ppm F Mean Body Weights in Kilograms 26 27 28 29 Sacrifice 2.11 1.82 2.16 1.82 2. 14 1.83 2.13 1.79 2. 13 1.79 Group Sex Week: Mean Body Weights in Kilograms 22 23 24 25 Sacrifice Aroclor 1242 M 100 ppm F 2.05 1.88 2.10 1.85 2.06 1.94 2.06 1.91 2.10 1.91 Group Sex Week: Aroclor 1254 M 1 ppm F Mean Body Weights in Kilograms 25 26 27 28 Sacrifice 2.26 1.93 2.23 1.89 2.22 1.92 2. 27 1.94 2,27 1.94 Group Sex Week: Mean Body Weights in Kilograms 26 27 28 29 Sacrifice Aroclor 1254 M 10 ppm F 2. 30 1.84 2. 30 1.83 2. 34 1.87 2.29 1.84 2.28 1.85 Group Sex Week; Mean Body Weights in Kilograms 22 23 24 25 Sacrifice Aroclor 1254 M 100 ppm F 1.83 1.81 1.76 1.79 1.83 1.76 1.97 1.66 1.96 1.68 ?o9 5 TOXSTUDIES0884 DaditiUkil BIO-TEST dakyialo'iici, 9nc. 22 TABLE XII continued TEST MATERIAL: Three Aroclors Recovery Diet Period Body Weight Data White Leghorn Chickens Group Sex Week: Mean Body Weights in Kilograms 26 27 28 29 Sacrifice Aroclor 1260 M 1 ppm F 2. 11 1. 84 2. 17 1.92 2..18 1.94 2. 18 1.92 ; 2. 18 1.82 Group Sex Week: Mean Body Weights in Kilograms 26 27 28 29 Sacrifice Aroclor 1260 M 10 ppm F Group Sex v .. Week: 2. 52 1.92 2.48 1.90 2. 50 1.96 2. 58 2.02 Mean Body Weights in Kilograms 25 26 27 28 2. 57 2.01 Sacrifice Aroclor 1260 M 100 ppm F 2.05 1.86 2. 12 1.90 2. 14 1. 90 2.20 1.93 2.26 1.95 ( " 0207096 TOXSTUDIES0885 J, s 3 1 - O 19 TABLE XII-A TEST MATERIAL: Three Aroclors White Leghorn Chickens Summary of 3ody Weight Data Group and Mean Body Weight Data - Kilograms Test Concentration 0 Day: Through Dosing: At Sacrifice: Material(ppm)MaleFemale_________Male_______FemaleMale______ Female I Cofitrol II Arbclor 1242 III Arbclor 1242 IV Arbclor 1242 V Aroclor 1254 VI Arbclor 1254 VII Aroclor 1254 VIII Aroclor 1260 IX Aroclor 1260 X Aroclor 1260 - 1' 10 100 1 10 100 1 10 100 1.27 . 1.27 1.28 1.28 1.28 1.28 1.28 1.28 1.28 1.28 0. 95 0.95 0. 95 0. 96 0. 96 0. 96 0. 95 0.96 0.95 0.95 . 2.43 2.68 2. 11 2, 05 2.26 2. 30 1.83 2. 11 2. 52 2.05 . 1.89 1.88 1.82 1.88 1. 93 1.84 1.81 1.84 1.92 1.86 2.44 2.70 2. 13 2. 10 2.27 2.28 1.97 2. 18 2.57 2. 26 . 1.88 1.86 1.79 1.91 1.94 1.85 1.68 1.82 2.01 1.95 -I'S oNt rws> >0 TOXSTUDIES0886 $ndtt&btial B I O - T E S T jta&Mai&ii&i, 9nc. 24 2. Food Consumption The food consumption data for the parent generation are ' presented in Table XIII. ' The chickens fed Aroclor 1242 at 100 ppm or Aroclor 1254 at 100 ppm ate less than those fed the control diet. 02070W TOXSTUDIES0887 Qnduifotal B I O * T E $ T Jafartafatiei, 9nc. 25 TABLE XIII TEST MATERIAL: Three Aroclors Average Food Consumption in grams/bird/day White Leghorn Chickens 1242 1242 1242 1254 1254 1254 Week Control 1 ppm 10 ppm 100 ppm 1 ppm 10 ppm 100 ppm 1260 1260 1260 I ppm 10 ppm 100 ppm Test Period i 67 66 78 63 77 77 2 90 67 81 70 82 72 3 97 98 90 77 95 88 4 113 136 101 82 113 107 5 89 110 94 6 147 134 121 61 86 93 95 109 120 7 117 143 120 77 99 111 8 131 125 109 90 9 126 156 132 99 10 232 204 204 . 176 108 129 206 130 127 210 n 172 166 166 175 151 144 12 134 132 140 94 126 114 13 144 136 156 97 148 139 14 135 138 160 78 15 117 118 147 98 16 123 167 185 111 134 140 129 126 170 171 17 121 137 127 18 109 110 111 19 167 149 131 20 86 100 39 31 23 62 20 128 99 102 76 115 139 69 79 21 126 108 99 101 22 266 154 154 100 128 95 117 169 23 247 217 256 - 87 33 24 249 198 197 25 190 156 167 . 134 124 171 102 26 148 144 - - - 122 Recovery Period 22 146 - - 23 - - - 118 -- 24 - - - 126 - 25 - _ 140 111 126 - 26 134 135 135 93 130 in 27 205 169 155 _ 127 177 28 136 104 151 29 257 226 154 _ - 145 168 160 140 30 256 161 * 153 80 54 90 107 80 99 137 140 128 171 164 113 78 74 76 98 38 68 14 28 94 80 - - - - 97 106 148 97 90 - - 82 39 99 90 80 135 141 146 142 254 186 163 162 139 125 178 129 106 138 100 149 150 130 148 143 139 78 66 54 88 98 86 102 95 100 87 107 132 103 87 117 145 154 148 192 221 157 174 128 131 134 148 130 162 126 108 141 153 113 129 103 106 128 129 94 88 101 120 123 120 138 207 135 120 133 191 71 - 0207099 - - - 92 142 154 143 143" - - - in 187 165 159 179 - - 121 145 148 143 161 - TOXSTUDIES0888 Dnduiktel B I O - T E S T lahywt&'Uei, $nc. 26 3, Mortality and Reactions The parent generation mortality chronology is presented in Table XIV. Autopsy of birds dying on test revealed malnutrition and extensive growths on kidneys, gonads, liver, or heart. These ani mals exhibited anorexia and grew progressively weaker until death. (' OZ07100 TOXSTUDIES0889 9tidnst'Ual B I O - T E S T J*<ritMafoUe4-, Dnc. 27 TABLE XIV TEST MATERIAL: Three Aroclors Mortality Data White Leghorn Chickens 1242 1242 1242 Week Control 1 ppm 10 ppm 100 ppm 1254 1254 1254 1260 1260 1260 1 ppm 10 ppm 100 ppm 1 ppm 10 ppm 100 ppm Test Period Mortality During Week Indicated 10 0 0 0 000 00 0 20 0 0 0 000 000 30 0 0 0 000 000 40 0 0 0 000 000 50 0 0 0 000 000 6 0 0 0 IF 0 0 0 000 7 IF 0 0 0 1M 0 IF IF 0 0 8 IF. 1M 0 IF IF 0 IF 0 IF 0 0 90 0 0 2F IF 0 IF 000 10 0 0 0 IF, 1M 0 0 0 0 00 11 0 IF IF 0 000 IF 0 0 12 0 0 2F IF IF IF 2M 0 00 13 0 0 IF 0 0 IF 0 0 0 1M 14 0 IF 0 3F 000 000 15 IF 0 0 0 0 IF IF 0 IF 0 16 IF 2F IF 0 000 000 17 0 0 0 IF 0 0 2F 0 0 0 18 IF 0 0 0 00 0 000 19 IF 0 0 0 00 IF IF 0 0 20 IF 0 0 0 1M 0 IF 0 IF 1M 21 0 0 0 1M IF 0 0 0 0 IF 22 0 0 0 0 IF 0 IF 0 00 23 1M 0 0 - 00- 0 IF IF 24 IF 0 0 - 2F 1M - 2F. 1M IF IF 25 0 0 0 - 00 . 00_ 26 0 0 - .- -0- 00- Recovery Period Mortality During Week Indicated 22 - - - 0 ._0 __ 23 - - - 0 0 __ 24 - - - IF - _ 0 _ _ 25 - - 0 0 0-0 _ _0 26 0 0 0 0 0 IF 0 000 27 0 0 0 - 0 0 - 0 0 0 2B 0 0 0 - '0 0 . 000 29 0 0 0 30 0 0 - _ - 00 _ -0- 000 00 0207101 TOXSTUDIES0890 $ttdnihtol B1 O TEST Jtti&iai&iiei, 28 4. Gross Pathology The results of the gross pathological examinations at sacrifice are presented in Tables XV through XXIV. The results of the gross pathological examinations at recovery sacrifice arc presented in Tables XXV through XXXIV. 0207102 TOXSTUDIES0891 jtnduifoial B I O - T E S T Jakyiak'U&i, 9*ic. 29 Animal Number and Sex 218-M 733-F 790-F 837-F 933-F 938-F 980-F B3-F TABLE XV TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Control Group Results of Gross Pathological Examination Normal Malnutrition - Small, diffuse growth on liver Malnutrition, Pericarditis, Myocarditis. Not Producing mature ova. Normal Normal Normal Normal Normal 0*0*103 TOXSTUDIES0892 QndiUUtol BIO-TEST J?a-&o-'uikfiie-d, $nc. 30 Animal Number and Sex 212-F 226-M 798-F 811-F 894-F 949-F 981-F 423-F 758-F 816-F TABLE XVI TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 1 ppm Results of Gross Pathological Examination Normal Normal Normal Malnutrition - Massive ovarian tumor. Many small tumors on kidneys. 3 cm. fatty encapsulated ball of pus in omentum. Normal Normal Normal Normal Normal - 0207104 TOXSTUDIES0893 frtJuibUal B I O - T E 5 T Jal^aiyUeS, 9*tc. 31 Animal Number and Sex 203-M 230-M 763-F 862-F 970-F 988-F 994-F 925-F 828-F TABLE XVII TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 10 ppm Results of Gross Pathological Examination Normal Normal . Normal Normal . Normal One leathery and one hard shell egg in peritoneal cavity Malnutrition. Ovary and oviduct filled with consolidated white material. Small pale spots on kidneys. Normal Normal ~ 0207105 TOXSTUDIES0894 frufa-ilrfal B I O - T E $ T Xabiyiatjyuei, jW 32 2 i o (M Animal Number and Sex 770-F 96 9-F 772-F 876-F 707-F TABLE XVIII TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 100 ppm Results of Gross Pathological Examination Omental fat depleted. Malnutrition. Not producing mature ova. Thin, semi-transparent ventricle wall. Normal Normal Normal Fat depletion. Ovum in peritoneal cavity. 0ino6 TOXSTUDIES0895 fyiJiiittJal BIO-TEST JaitMatoUeA, $ttc. 33 Animal Number and Sex 233-M 997-F . 877-F 976-F 704-F 775-F 916-F 923-F TABLE XIX TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 - 1 ppm Results of Gross Pathological Examination Normal Normal Normal Normal Normal Normal . Normal Malnutrition, Enlarged, fatty infiltrated spleen. Encapsulated tumors of breast muscle. Fatty infiltrated liver. Encapsulated chick embryo being absorbed. 0207107 TOXSTUDIES0896 $i'dultsual B I O - T E S T li.i/t&iai&ueS' 9*t&. 34 Animal Number and Sex 244-M 715-F 735-F 736-F 813-F 858-F 866-F 902-F 881-F TABLE XX TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 - 10 ppm Results of Gross Pathological ' Examination Normal Malnutrition. Not producing mature ova. Malnutrition. Not producing mature ova. Several _ myocardial and hepatic fatty tumors. Normal Normal Normal Normal Not producing mature ova. Normal 0207108 TOXSTUDIES0897 Juifalal B I O - T E S T Jtakytaiiyiic-i, 9hc-. 35 f Animal Number and Sex 227-M 723-F ' 765-F 826-F 832-F 848-F 983-F TABLE XXI TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 - 100 ppm Results of Gross Pathological Examination ` Malnutrition, Pericarditis, Myocarditis. Hepatic consolidation and hemorrhage. Normal Normal Myocarditis. Pericarditis, Pale kidneys. Resorbing ovum in peritoneal cavity. Weak wall of left ventricle. Malnutrition. Not producing mature ova. Ovary contains many follicles filled with blood or resorbing. Normal 020710') TOXSTUDIES0898 piiJirtfalal B 1 O T E $ T J.abo'iaio'iiei, Due, Animal Number and Sex 222-M 829-F 785-F 792-F 800-F 794-F 731-F 944-F TABLE XXII TEST MATERIAL: Three Aroclore Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 1 ppm Results of Gross Pathological Examination Normal Normal Not producing mature ova. Normal Normal Normal Normal Normal 36 . 0207110 TOXSTUDIES0899 DnJuit'Uai BIO-TEST ja&&iaivUcl. $ho. 37 Animal Number and Sex 249-M 239-M . 915-F 903-F 985-F 999-F 922-1' 789-F 932-F 732-F TABLE XXIII TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 10 ppm Results of Gross Pathological Examination Normal Normal Normal Normal Normal Patent right oviduct filled with 40 ml, white granular -material and covered with growths, 3 fatty encapsulated growths on liver. Normal Normal Malnutrition. Enlarged liver. Ovarian and renal growths. Normal 02071*1 TOXSTUDIES0900 9nd\tsUal B1 O - T E S T 9nc. Animal Number and Sex 211-M 721 -F 806-F 984-F 745-F 896-F 780-1' 979-F 853-F TABLE XXIV TEST MATERIAL: Three Aroclors Residue Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 100 ppm Results of Gross Pathological Examination Normal Normal Malnutrition Normal Normal Normal Normal Fatty growths on spleen and heart. Normal 38 0207112 TOXSTUDIES0901 B I O - T E S T Xalftyiat&rfvl, 9ho. Animal Number and Sex 224-M 987-F 717-F 838-F 912-F 992-F TABLE XXV TEST MATERIALS: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Control Group Results of Gross Pathological Examination Normal Normal Normal Fatty growths throughout kidneys Normal Normal 39 ozom3 TOXSTUDIES0902 UtidaiVUal B I O - T E 5 T 9*ic. Animal Number and Sex 238-M 250-M , 703-F 752-F 782-F 840-F 867-F 709-F 897-F 963-F TABLE XXVI TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 1 ppm Results of Gross Pathological Examination Normal Normal Normal Normal Normal Normal . Fibrous growths in duodenal mesentery. Normal Normal Normal 40 sion" TOXSTUDIES0903 jlfufdiifaicU B IO - T E S T loiMatwii&i, 9m. 41 Animal Number and Sex 235-M 252-M 718-F 796-F 842-F 884-F 888-F 906-F 934-F TABLE XXVII TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 10 ppm Results of Gross Pathological Examination Several large, pale growths on kidneys. Normal Normal Normal 10 ml. fluid-filled vesicle in mesometrium, ' Rubbery-shelled egg in peritoneal cavity* Normal Normal Malnutrition. Mottled and fibrotic liver. Normal 0207115 TOXSTUDIES0904 ftaduitiial B I O - T E S T JaiuViabyii&L, 9n&. 42 Animal Number and Sex 225-M 7V6-F 742-F 869-F 964-F table xxviii TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1242 - 100 ppm Results of Gross Pathological Examination 3 small, white growths on heart. Normal Normal Normal 200 ml. fluid in peritoneal cavity. 25 ml. fluid in pleural cavity. Omentum fragmented and the omental fat necrotic. Ovary necrotic. Two dozen small white growths on heart. TOXSTUDIES0905 Unduihial B I O - T E S T ^a&oJiai&Ued, 9tic. Animal Number and Sex 206-M 7I4-F 810-F 833-F 864-F 891-F 900-F 910-F TABLE XXIX TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 - 1 ppm Results of Gross Pathological Examination Normal . Normal Normal Normal 3 ml. fluid-filled vesicle in mesometrium. Normal Normal Normal 43 G 0207117 TOXSTUDIES0906 tduifoial B I O T E S T 2a&&iaio'Uei, 9h&. ( Animal Number and Sex 217-M 245-M 778-F 801-F 7B6-F 702-F 847-F 720-F 713-F TABLE XXX TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 * 10 ppm Results of Gross Pathological Examination Normal Normal Normal Normal Normal Normal Normal Resorbing ovum in peritoneal cavity. Normal 44 t 0207118 TOXSTUDIES0907 SndMiVtial B I O - T E S T Xa&Ma&vUeA, Puc. 45 Animal Number and Sex 229-M 998-F 724-F 921-F 957-F 959-F 819-F TABLE XXXI TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1254 - 100 ppm Results of Gross Pathological Examination - Malnutrition. 50 ml. pericardial fluid. Enlarged, dark liver. . Malnutrition. Several small, white cardiac growths. Enlarged pale kidneys. No de tectable gall bladder. Not producing mature ova. Normal Normal Normal Normal 30 ml. yellow subcutaneous fluid. 100 ml. fluid in peritoneal cavity. 50 ml granular material and fluid in omentum. Enlarged, pale liver. Several small growths on heart. Enlarged ovary shedding immature ova. Malnutrition. 0207119 TOXSTUDIES0908 Stdailttal B I O - T E S T JatmaiMiel, }m. Animal Number and Sex 232-M 240-M 946-F 1000-F 913-F 885- F 757- F 729-F 759-F TABLE XXXII TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 1 ppm Results of Gross Pathological Examination Normal 3 cm. cage bruise (breast blister) Normal Normal . Normal Normal Normal Normal Resorbing egg in peritoneal cavity. 46 * 0207120 TOXSTUDIES0909 yrJaSfaiat B I O - T E S T Ja-knaio-tiei-, 9nc. Animal Number and Sex 243-M 215-M 716-F 784-F 748-F 849-F 919-F 879-F 839-F 701-F TABLE XXXIII TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 10 ppm Results of Gross Pathological Examination Small cage bruise (breast blister) 3 cm. cage bruise (breast blister) Normal Normal Normal Normal Resorbing ovum in peritoneal cavity. Normal Normal . Resorbing ovum in omentum. 47 0201121 TOXSTUDIES0910 $nduihlal D 1 O - T E S T Ja&Mat&tleS, 9tic. Animal Number and Sex 236-M 737-F 805- F 754-F 924-F 966-F 741-F 926-F B70-F 996-F TABLE XXXIV TEST MATERIAL: Three Aroclors Recovery Sacrifice Gross Pathology White Leghorn Chickens Aroclor 1260 - 100 ppm Results of Gross Pathological Examination Normal Resorbing ovum in peritoneal cavity. Resorbing ovum in peritoneal cavity. Normal Normal Normal Normal Normal Normal . Resorbing ovum in peritoneal cavity. 48 . 020712? TOXSTUDIES0911 Suduihlol B I O - T E S T Ja&Mat&u'cd; Stic. 49 5* Egg Production The weekly test period egg production is presented in Tables XXXV through XLIV. The weekly recovery period egg production is presented in Tables XLV through LIV. Egg production in groups fed Aroclor 1242 at 100 ppm or Aroclor 1254 at 100 ppm was much lower than the production of the control groups. After being on test for 12 or 13 weeks, a maximum of 40 to 43% of the hens in these two groups laid eggs. In the other groups about 90 to 100% of the hens laid eggs. r 0207123 <L TOXSTUDIES0912 !)nduiU<al B I O - T E S T daltjyuit^niei-, 9nc, 50 Test Week 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 21* 22 23 24 25 26 TABLE XXXV TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Control Group Number of Errs Laid Average Number of Egg Weight Errs Weighed (grams) 2 2 42. 9 4 3 39.4 27 27 42.4 41 41 42.7 72 72 44. 3 79 76 44.4 90 90 47. 3 92 91 48. 1 90 90 49.4 91 91 96 96 49.9 52. 5 91 91 52. 5 91 91 54.8 82 82 54. 5 75 75 55. 3 22 22 56.6 28 28 57. 1 28 28 57.7 24 24 58. 3 22 22 60.4 24 24 56.8 Percent Production 1.4 2.9 19. 3 30.6 56.3 62.7 71.4 73.0 71.4 72. 2 76.2 76. 5 81.3 74. 5 71.4 73.3 66.7 66.7 57. 1 59.5 80.0 * Five days. 02071M TOXSTUDIES0913 BI O - T E S T JafaMtoUei, $ho. 51 Test Week 4 5 6 7 8 9 10 11 12 13 14 15 16 17 IS 19 21* 22 23 24 25 26 TABLE XXXVI TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1242 - 1 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 5 9 15 35 69 108 108 97 . 99 97 101 102 94 94 100 89 30 32 40 37 42 38 3 9 15 35 69 104 107 94 96 96 100 100 94 93 100 89 30 32 40 37 42 38 42,0 34,3 40. 1 42.2 43.3 46.0 47. 2 47. 9 50.0 49.8 50.7 51.8 52. 5 52.9 53.4 53.8 55.0 57.4 54.9 56.6 59.2 57.6 Percent Production 3.4 6.1 10.2 23.8 46.9 73. 5 73.5 66.4 70.7 69.3 73,7 76.7 71.8 78.3 84.0 74.8 75.0 57.1 71.4 66.1 75.0 67. 9 * Five days. 02071?s TOXSTUDIES0914 B I O -T E S T Xalo'ioio-'uel, 9*tc. 52 Test Week 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21* 22 23 24 25** TABLE XXXVII TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1242 - 10 ppm Number of Eggs Laid . Number of Eggs Weighed Average Egg Weight (grams) 1 10 33 62 77 89 98 87 95 87 85 74 73 71 ' 73 68 25 39 34 38 17 i 10 32 62 76 89 97 86 94 83 84 74 73 71 73 68 25 39 34 38 17 33.8 34.9 42.3 43. 1 45. 0 47. 0 48. 7 50.8 49.4 50.8 51.6 52.2 53. 1 53.2 55. 2 54.8 ' 56. 4 56.0 58. 2 59.2 59.9 Percent Production 0.7 7. 1 23.6 44.3 57. 9 66.9 74.7 69.0 79.2 77.0 75.9 67.3 70.9 72.4 74.5 69.4 71.4 79.6 69.4 77.6 84.0 * Five days, ** Three days. 02on?b TOXSTUDIES0915 Stufuihlal B I O - T E 5 T JaA&iat&ued, 9nc. 53 Test Week 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21* 22** TABLE XXXVIII TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1242 - 100 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 33 0 0 55 77 15 15 33 33 34 34 32 32 40 40 36 36 23 22 23 23 27 27 18 18 14 14 11 11 99 10 10 37.2 - 34.5 42.7 41.0 46.6 48.3 46.8 47.5 49.5 49.9 48.3 49.9 51.2 52.2 52.8 51.6 53.0 Percent Produ ction 2.1 0 3.7 5.3 12.1 28.7 31.8 31.7 40.8 37. 5 29. 5 29.9 35. 1 25.0 20.0 15.7 18.0 16.6 * Five days. ** Six days. TOXSTUDIES0916 9r.diiSfoJal B I O - T E S T J.al&'iai&xt&i, 9nc. 54 Test Week 6 7 8 9 10 11 12 13 14 15 16 17 18 19 21* 22 23 24 25** TABLE XXXIX TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1254 - 1 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 7 32 68 81 98 91 100 92 105 108 99 105 102 99 68 80 64 73 21 . 7 32 68 81 97 91 99 92 103 107 99 105 102 99 68 80 64 73 21 38.4 40. 1 42.6 44.9 46.3 48.2 48.9 51.5 50.2 51.7 52.0 53. 5 56.1 52. 3 54.3 56.1 56.4 58.4 59.9 Percent Production 5.0 22.9 48.6 57.9 73.7 68.4 75.2 72.4 83.3 85.7 78.6 83.3 81.0 78.6 75.6 67.8 57.1 67.0 47.7 * Five days. ** Three days. 020712# TOXSTUDIES0917 $iufuihlal B I O - T E S T Jakrtato-iiei, 9nc. 55 TABLE XL TEST MATERIAL: Three Aroclora Test Period Egg Production White Leghorn Chickens Aroclor 1254 - 10 ppm Test Week 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21* 22 23 24 25 26** Number of Eggs Laid 2 25 37 56 84 88 87 86 88 88 89 86 93 80 85 88 64 84 ' 62 48 58 54 Number of Eggs Weighed 2 25 37 56 82 88 87 86 86 86 88 86 93 80 85 88 64 84 62 48 58 54 Average Egg Weight (grams) 36. 5 41.9 41.0 42.3 44.9 45.2 48.0 50.7 51. 1 51.2 52.0 52. 1 51.2 52. 3 54.0 54.1 52. 0 55.6 55.2 54.0 56.0 55.9 Percent Produ ction 1.4 17.9 26.4 41.5 63.2 66.2 65.4 65.2 69.8 73.9 75.4 76.7 83.0 71.4 75.9 78.6 80.0 75.0 55.4 42.9 51.8 56.3 * Five days, ## Six days. 0201^ TOXSTUDIES0918 DtiJnStMal B I O - T E S T Jalw'ujfo'Uei, 9nc. 56 Test Week 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21* 22** TABLE XLI TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1254 - 100 ppm Number of . Number of Eggs Laid Eggs Weighed Average Egg Weight (grams) 2 11 28. 43 54 . - 44 44 39 36 27 28 15 15 7 ' 15 10 6 2 a 28 42 53 43 43 39 36 27 28 15 15 6 15 10 6 32.3 36.4 40.0 44.7 45.1 45.5 46.0 46.2 47.9 47.9 45.9 47.5 48.0 49.8 50.7 50.8 51.0 Percent Produ ction 1.4 7.9 21. 1 33.6 42.9 34.9 34.9 31.0 28.6 21.4 23.1 12.6 14.0 6.9 15.3 15.4 7.7 * Five days. ** Six days. 0207130 TOXSTUDIES0919 B I O - T E S T lalo^aUiiei, 9nc. 57 Test Week 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21* 22 23 24 25 26** TABLE XLII TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1260 - 1 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 11 33.6 23 23 34. 5 34 34 40.4 64 64 43.4 76 75 45.7 83 83 46.6 89 89 90 90 82 81 48.3 50.4 51.3 86 85 50.9 84 84 52.1 72 72 52.8 81 79 68 68 53. 1 55.4 75 75 48.6 73 73 55.9 59 59 50.4 59 59 57.8 68 68 56.8 60 60 58.6 70 70 . 59.2 50 50 59.2 Percent Production 0.7 16.4 24.5 48.1 60.3 65.9 70.6 75.0 68.9 72. 3 70.6 60. 5 68.1 57, 1 64.1 65.2 73.8 52.7 60.7 57.7 71.4 59-5 * Five days. ** Six days. 0207131 TOXSTUDIES0920 hufuiUlal BI O - T E S T 9nc. 58 TABLE XLIII TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1260 - 10 ppm Test Week Number of Eggs Laid ' Average Number of Egg Weight Percent Eggs Weighed(grams)______ Production 58 6 18 7 36 8 51 9 85 10 110 11 97 12 99 13 99 14 106 15 101 16 84 17 94 18 100 19 98 21* 67 22 83 23 83 24 76 25 73 26 78 8 41.6 5.7 17 39. 3 12.9 36 37.8 25.7 51 43.3 36.4 85 44.6 60.7 110 45.3 78.6 97 46.6 71.9 99 48. 1 74.4 98 48.7 74.4 105 50. 3 75.7 101 50.3 73.2 84 51.2 63.2 92 51. 5 70.7 100 52. 1 75.1 98 52. 1 73.7 67 50.6 74.4 83 53.4 65.9 83 54. 1 66.9 76 56.3 63.9 73 56.6 64.6 78 56.4 69.6 * Five days. 0207132 TOXSTUDIES0921 Duduifoial B I O - T E S T Ja-fi&iat&'uei, 9nc. 59 Test Week 6 7 8 9 10 11 12 18 14 15 16 17 18 19 21* 22 23 24 25** TABLE XL1V TEST MATERIAL: Three Aroclors Test Period Egg Production White Leghorn Chickens Aroclor 1260 - 100 ppm Number of Eggs Laid . Number of Eggs Weighed Average Egg Weight (grams) 3 3 39.1 20 20 38.1 51 51 41.6 82 81 45.9 98 97 47.0 108 104 47.9 109 107 49. 5 in in 49.8 111 no 50.8 113 113 50.9 100 100 50.6 118 117 55.0 105 105 54.0 106 106 53. 0 69 69 54.0 93 93 53. 9 73 73 * 55.3 76 76 56. 1 20 20 57.3 Percent Production 2. 1 14.3 36.4 58.6 70.0 77.1 77.9 79.3 79.3 80.7 71.4 84.3 75.0 75.7 70.4 69.9 55.7 60.3 37.0 * Five days. ** Three days. 0207133 TOXSTUDIES0922 huiuihial B I O - T E S T Xal&iatoucA., $4t&. 60 TABLE XLV TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Tost Week Number of Eggs Laid Control Group Numbe r of Eggs Weighed Average Egg Weight (grams) Percent Production 26* 27 28 29 30 ' 31* 3 28 28 23 25 ... 3 3 28 . 28 23 25 3 57. 5 59.9 60.0 59.8 60.2 58.9 60. 0 80. 0 80. 0 65.7 71.4 60. 0 * One day. 0207134 TOXSTUDIES0923 fatal B I O T E S T J.afayiaia'iicA, $hc. 61 Test Week 27 28 29 30 31* TABLE XLVI TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1242 - 1 ppm Number of EEC's Laid Number of Eggs Weighed Average Egg Weight (grams) 42 42 57.6 48 48 59. 5 46 46 59.2 47 47 59. 0 10 10 59.1 Percent Production 75. 0 85. 7 82. 1 83. 9 62. 5 * Two days. TOXSTUDIES0924 thutuifalal BIO-TEST Jtaky'ialo'iiM. $nc. 62 Test Week 26 27 28 29 30* TABLE XLVII TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1242 - 10 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 33 33 58. 3 22 22 58. 1 30 30 58.5 31 31 57.6 3 3 59.2 Percent Production 67.3 44. 9 61.. 2 63.3 42.9 * One day. 0lyi3b I TOXSTUDIES0925 B I O - T E S T Ja/io-iaUiiM, Due. 63 TABLE XLVIII TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1242 - 100 ppm Test Week Number of Ekes Laid 22* 23 24 25 26 . 27* ' 0. 9 7 10 7 0 Number of Ekes Weighed 0 9 7 10 7 .o Average Egg Weight (grams) 0 60. 9 61. 8 59. 5 55.8 -_ Percent Production 0 25.7 21., 2 35.7 25.0 0 * One day. *-.v. 0207137 TOXSTUDIES0926 B I O - T E S T akyiafaik4, $nc. 64 TABLE XLDC . TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1254 - 1 ppm Test Week Number of Eer9 Laid Number of Ekes Weighed Average Egg Weight (grams) Percent Production 26 41 41 58.4 83.7 27 40 40 59.2 81.5 28 37 37 58.5 75. 5 29 38 38 54.7 77.6 30* 4 4 58.3 57. 1 * One day* 0207138 TOXSTUDIES0927 BIO-TEST Johyiato-iiM, $ho. 65 TABLE L TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1254 - 10 ppm Test Week Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 26* 27 28 29 30 ' 31* 3 39 41 42 .39 3 3 39 41 42 39 3' 54. 3 57.8 58.2 58.6 58. 7 56.7 Percent Production 37.5 76. 5 83.7 85.7 79.6 42. 9 * One day. 0207139 TOXSTUDIES0928 $nditiVilal B I O - T E S T JotiMatoiteS, 9nc. 66 Test Week 22* 23 24 25 26 27** TABLE LI . TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1254 - 100 ppm Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 4 4 47.0 14 14 50. 1 18 18 52.9 14 14 54.0 9 9 52.0 6 6 54. 2 Percent Production 66. 7 33.3 42; 9 33.3 21.4 50. 0 * One day. ** Two days. 0207140 TOXSTUDIES0929 faufitifoial BIO-TEST Ja&o'iat&iifl, 9nc. 67 TABLE LII TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1260 - 1 ppm Test Week Number of Eggs Laid Number of Eggs Weighed Average Egg Weight (grams) 26* 5 27 37 28 36 29 30 ` 33 .35 31* ' ... 5 5 37 . 36 33 35 5 62. 5 60.7 61.0 60.7 61.9 61.1 Percent Production 71.4 7 5. 5 73w 5 67.3 71.4 71.4 * One day. 0207141 TOXSTUDIES0930 Pitiltiihinl B I O - T E $ T J<ihyiai&\ieA, 9nz 68 Test Week . 27 28 29 30 31* TABLE LIU TEST MATERIAL: Three Aroclors Recovery Period Egg Production White Leghorn Chickens Aroclor 1260 - 10 ppm Number of Eggs Laid Average Number of Egg Weight Eggs Weighed ' (grams) 48 48 55.6 44 44 56. 5 43 43 57.5 45 45 57. 5 13 13 57.5 Percent Production 85.7 78.6 76.8 80.4 81.3 * Two days. '020714? TOXSTUDIES0931 $n4uiiMal B 1 O - T E S T Jtal&'iai&'ifai, 9no. 69 Test Week 26 27 28 29 30* TABLE LIV TEST MATERIAL: Three Aroclora Recovery Period Egg Production White Leghorn Chickens Aroclor 1260 - 100 ppm Number of Eggs Laid 44 52 46 45 7 Number of Eggs Weighed 44 52 46 45 7 Average Egg Weight (grama) 57.0 58.2 57.3 57.4 58.6 Percent Production 69.8 82. 5 73,0 71.4 77.7 * One day. 02071<t3 TOXSTUDIES0932 Undtiikial B I O - T E S T Ja&yiato-'U&t, 9*tc. 70 Eggs 1, Egg Production and Quality The test period weekly egg production is presented in Tables XXXV through XLIV. The recovery period weekly egg production is presented in Tables XLV through LIV. The candling results for a number of seven-day periods are presented in Table LV, with the total number of eggs for each defect tested according to the following code: Br = Broken in nest Mbr = Broken during handling Cr = Cracked shell Dy = Double yolk present Ny = No yolk present Ck e Check shell Sf = Soft or misshapen shell Sp = Blood or meat spot in egg Nas = Air bubble defective or absent Where multiple defects occurred, the egg is tabulated under the defect highest in the list above, TOXSTUDIES0933 itX L O iO c 'f w /'*yy Sa J. -1 1a p>fpuf<>(0 TABLE LV TEST MATERIAL: Three Aroclors Egg Candling Results Summary White Leghorn Chickens Number of Eggs For Each Type of Defect GroupBr Mbr Cr Dy Ny Ck Sf Sp Nas Control 0 1242 1 ppm ' 3 * 1242 10 ppm 1 1242 100 ppm 0 1254 1 ppm 1 1254 10 ppm 2 1254 100 ppm I 1260 1 ppm 1 1260 10 ppm 3 1260 100 ppm 2 0 16 6 0 . 32 6 0 20 4 0 1. 4 0 16 4 1 23 6 0 70 1 34 1 1 35' 5 0 56 10 0 68 . 0 1 0 79 0 . 4 0 89 0 0 1 15 0 0 0 110 0 3 1 98 0 0 0 27 1 0 0 12 0 2 0 78 0 0 0 116 0 0 9 1 1 0 0 0 0 2 0 0 Total Defective Eggs 100 125 115 21 134 131 36 .53 122 .. 184 Total Eggs Percent Defective Eggs 435 23 478 26 444 26 111 19 516 26 521 25 100 36 443 12 477 26 545 34 TOXSTUDIES0934 Pmiitii'Ual Bl O - T E 5 T Jakyiafoue*. 9*tc. 72 2* Hatch ability The hatch data are presented in Table LVI, * Examination of the unhatched eggs revealed the data presented in Table LVII. ** Data from the gross pathological examination of chicks at sacrifice after the 30-day observation period are presented in Tables LVIII through EXV. * There was poor hatchability of eggs from hens fed Aroclor 1242 at 10 or 100 ppm or fed Aroclor 1254 at 100 ppm. ** In eggs from hens fed Aroclor 1242 at 10 or 100 ppm, there were a large number of embryos in the one to three cm size. - 0207,'b TOXSTUDIES0935 HtukiiUial B I O T E S T Hue. 73 TABLE LVI TEST MATERIAL: Three Aroclors Hatch Data White Leghorn Chickens Group Number of Number of Chicks Eggs Set Hatched Hatching Percent Average Weight After Day 1 (grams) Agerage Weight After Day 30 (grams) Control 161 105 65.2 39. 1 248 1242 1 ppm 110 73 66.4 36. 7 281 1242 10 ppm 161 28 17.4 34.6 232 1242 100 ppm 1254 1 ppm 44 105 0 0- 68 64.8 34. 2 251 1254 10 ppm 120 78 65.0 32.6 260 1254 100 ppm 1260 1 ppm 38 127 0 0' - 88 69.3 39.0* 295* 1260 10 ppm 135 94 69.6 34.2** 282** 1260 100 ppm 120 74 61.7 35.9 264 * Does not include seven chicks which crossed into 1242 10 ppm at hatching. ** Includes seven chicks which crossed from 1242 1 ppm at hatching. o?on<i TOXSTUDIES0936 TABLE LYE TEST MATERIAL: Three Aroclors Examination of Unhatched Eggs White Leghorn Chickens Grout) Control Total Unhatched Number of Eggs Per Group Broken Not Embryo Embryo Embryo Shell Fertile Decomposed < 1 cm 1-3 cm Chick Chick 1/2 Term 3/4 Term Chick Chick Partially Full Out of Term Shell 56 0 3 1 17 6 1 892 1242 1 ppm 37 1 7 1242 10 ppm 133 0 8 2 1 72 9 73 3 11 3 12 4 22 0 5 1242 100 ppm 43 1 10 0 11 20 1 000 1254 1 ppm 1254 10 ppm 37 42 0 10 0 13 0 1 6 8. 1 '3 4 1 19 6 13 2 1 1254 100 ppm 1260 1 ppm 1260 10 ppm 1260 100 ppm 38 39 41 46 1 13 13 03 0 10 0 0 0 1 14 9 5 12 79 92 0 0 2 7 01 1 14 8 10 0 13 0 3 2 4 CO O m to ! OZO?!* TOXSTUDIES0937 $tt<f*rtVUal B I O - T E S T Jaioiatoiiei, Dkc. 75 Number of Animals 93 z \ 1 1 TABLE LVIII TEST MATERIAL: Three Aroclors Control Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination . Normal : Possible fatty myocardiac growth Deformed feet Deformed leg , Z. 5 cm round hepatic growth with greensheen 02071*9 TOXSTUDIES0938 Undnifoial BIO-TEST Xo&o'iai&Uci., Une. 76 Number of Animals 70 TABLE LIX TEST MATERIALS: Three Aroclors 1242 1 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination . Normal OJC'*0 TOXSTUDIES0939 Qiufult'Ual B I O - T E S T JaluytaUnieS, 9nc. 77 Number of Animals 20 TABLE LX TEST MATERIALS: Three Aroclors 1242 10 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination Normal 02071^1 TOXSTUDIES0940 fyiduifoial B I O - T E S T 2a'iMatvUcl, 9hc. 78 Humber of Animals 56 1 1 1 TABLE LXI TEST MATERIALS: Three Aroclors 1254 1 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination Normal Down on side. Both feet curled inward Only wings feathered TOXSTUDIES0941 'J'-l'il B I O - T E S T Jakvialcrtici, Ptic. 79 ... TABLE LXII c TEST MATERIALS: Three Aroclors 1254 10 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Number of Animals Results of Gross PatHolgical Examination 56 Normal : 2 Deformed feet i Unable to extend right leg 1 Dark left testicle I Possible fatty tumors on spleen (" C 0207199 TOXSTUDIES0942 Muifoial B I O - T E S T lab.yvitQ'ucl' Hue. 80 Number of Animal 8 74 1 TABLE LXIII TEST MATERIALS: Three Aroclors 1260 1 ppm Gross Pathology 30-Day Chicks While Leghorn Chickens Results of Gross Pathological Examination Normal Possible kidney tumors ozon^ TOXSTUDIES0943 De.&iiUial B I O - T E S T XaJ&iai&u&S., 9tic. 81 Number of Animals 90 1 1 TABLE LXIV TEST MATERIALS: Three Aroclors 1260 10 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination Normal Feet curled in Slight discoloration of left testicle 0207155 TOXSTUDIES0944 fauktiUial B I O - T E $ T Jafaiafoii&t, $nc. 82 Number of Animals 60 2 1 TABLE LXV TEST MATERIALS: Three Aroclors 1260 100 ppm Gross Pathology 30-Day Chicks White Leghorn Chickens Results of Gross Pathological Examination Normal Feet curled under Not eating. Still has chick down. 02071^6 TOXSTUDIES0945 Sadtiihiai B I O T E S T XabviaiyUe'i, 9no. 83 3. Physical Characteristics of Eggs Data on the specific gravity of intact eggs are summarized in Table LXVI. * Data on the average egg shell thickness (without membrane) at the equatorial circumference are presented in Table LXVII. Three measurements were determined for each egg.** * There were no significant differences in specific gravity among eggs from treated or from control hens. ** The egg shells from hens fed Aroclor 1242 at 10 or 100 ppm or fed Aroclor 1254 at 100 ppm were not as thick as the shells of eggs from hens fed the control diet. TOXSTUDIES0946 851*020 wtf 'tw q v w fD ? x s a - o 19i TEST MATERIALS: Three Aroclors Egg Specific Gravity- White Leghorn Chickens Group Normal Eggs Average Number Specific Gravity Defective Eggs Average Number Specific Gravity Total Eggs Average Nuftibe r Specific Gravity Control 81 1.070 83 1.079 167 1.074 1242 1 ppm 88 1.074 99 1.072 187 1.073 1242 10 ppm 89 1.070 94 1.069 183 1.070 1242 100 ppm 160 1.064 19 1.065 35 1.065 1254 1 ppm 95 1.071 117 1.068 212 1.070 1254 10 ppm 109 . 1.070 112 1.070 221 1.070 1254 100 ppm 12 1.065 29 1.064 41 1.064 1260 1 ppm 85 1.070 115 1.070 200 1.070 1260 10 ppm 93 1.070 80 1.069 173 1.070 1260 100 ppm 96 1.070 135 1.063 231 1. 068 09 TOXSTUDIES0947 $*Lutm al B IO -T E S T A. Group Control 1242 1 ppm 1242 10 ppm 1242 100 ppm 1254 1 ppm 1254 10 ppm 1254 100 ppm 1260 1 ppm 1260 10 ppm 1260 100 ppm TABLE LXVH TEST MATERIALS: Three Aroclors Egg Shell Thickness ' White Leghorn Chickens . Normal Eggs Defective Eggs ` Average Average . Thickness Thickness Number. (1/1000 in.)Number(1/1000 in.)Number(1/1000 in) Total Eggs Average Thickness 72 14.58 71 14. 15 143 14. 37 75 14.37 89 14. 04 164 14.19 78 13.85 82 13.48 160 13.66 14 12. 07 15 11.62 29 11.84 73 14. 32 no 14.03 . 183 14.15 120 12.79 127 14.40 247 13.62 12 10. 25 35 11.10 47 10.88 86 14.56 143 14. 17 234 14.28 91 . 14. 75 116 14.93 98 13.99 179 14. 11 207 14.85 277 14.07 (,4U0Z<^ TOXSTUDIES0948  B I O - T E S T Jta&oiaUtiei, Sue. -I 86 4. Residue Studies One fourth of all nondefective eggs during the test period were frozen and shipped to Monsanto for residue analysis. During the recovery period a minimum of ten eggs per group per week were quick frozen and shipped to Monsanto for analyses. o2on>0 TOXSTUDIES0949