Document Rj55pZjVLMy45y0eoLZvXLQQ7

O R iU crO sn MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE______________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, FC-143 or as the major component of FC-118. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS# 3825-26-1) Remarks: The test sample is FC-118. It's purity was not sufficiently characterized, though current information indicates is 20% FC-143 in 80% water. The 3M product lot number was not noted. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD:___________________________________________________ Method: Microbios Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Stock and test solution preparation: A primary 10 g/L stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was 6.0 (no adjustment) and an osmotic adjustment was made using 200 mg NaCI in 10 mL stock solution. Appearance of test solutions was noted as "clear and solorless". All test solutions were made by proportional dilutions. Exposure vessels: 3 mL Cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss. RESULTS___________________________________________________ 004028 Nominal concentrations: Blank control, 625, 1250, 2500, and 5000 mg/L Element value: 5-minute EC50 = 4460 (4020 - 4950) mg/L 15-minute EC50 = 3360 (3090 - 3641) mg/L 30-minute EC5o = 3150 (2910 - 3420) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. CONCLUSIONS______________________________________________ The FC-118 30-minute EC50for Photobacterium phosphoreum was determined to be 3150 mg/L with a 95% Confidence Interval of 2910 to 3420 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES_______________________________________________ This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number P1626. OTHER____________________________________________________ Last changed: 5/25/00 004029 BESTCOPYAVAIUBil 004030 BtSlCON H cJr Z JR, & P l I o U k 'b F IL E F C - llf.K K F161'6 ' t i t. F O T O < O A 'tA FvFFFOF-T O-A'al.c Ti.?**- Lm e : 1 5 m i n u ts> NOMKiifk ...__ _ Con t r o l Ccir>t r 1>1 10 l T - 9*j . 4 4 / / 9 . i 9.'" ,. Si ' i 7 9 , 9 0 r it jm o i i*. : Ac h > t triHri t ! N C 1 <:>jo, - . .... ........... . ..... Vo c.V j . 6rV t<vs ^ C fir/S A M M A ______ _ UC r>-*> Loas * , V O , &?*.** H ' . 0 2 9 9 n -- t 0 7 6 '/ - V.i V / / . . 8 9 . 1 7 / 40 4 .4o/ 04. . ! . 4 7 / 19.. 4 7 c> 8 / . 7 9 / 4 5 . ,7 v i 97. ,, * y ; / 7 7 . 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