Document Rj4d04Bp6beXo38kNOdwOY25k
BIODEGRADATION
TEST SUBSTANCE
Identity:
Perfluorooctylsulfonate, didecyldimethylammonium salt; also be referred to as Fluoroalkyl ammonium derivative.
may [1-
Decaminium, N-decyl-N,N-dimethyl-, salt with
11,22,3,3,4,4,5,5,6,6,7,7,8,8 8-heptadecafiuoro-1-
octanesulfonic acid (1:1), CAS # 251099-16-8]
LR-e1m4a3r9k4s:referTrehdet3o Mbyprtohdeutcetsitolnalbootrantuomrbyears
was Lot P3025.
1. The test sample The sample was
is
labeled in water
F-11615, Lot 1. (approximately
The test sample is a mixture of the test 30-40% test substance, 60-70% water,
substance and 0-5%
of residual perfluorochemicals). The test sample appears to be a
All values reported relate to this 2-phase dispersion (clear liquid
mixture. with
opaque solid) `were made to
which adjust
rapidly for the
saectpuaarlatceosncaefntterraatgiiotnatoifont.heNtoesctaslcuublsattainoncse
in
the test sample.
METHOD:
Method: OECD Method 301D
Type: Aerobic
GLP: No
Year completed: 1987
Contact time: 28 days Inoculum: Polyseed-
NX
(with
nitrification
inhibitor),
lot
A306,
Polybac
Corp, Bethlehem, PA
RESULTS
COD: 125,000 mg Oy / kg chemical
cBOD Exposure period, Day
[51 [0
20 28
First Replicate CBOD
Mean Value
(mg/kg)
<40,000 <40,000 <40,000 55,038
Second Replicate CBOD
Mean Value _ (<m4g0/,k0g0)0
<40,000 <40,000 1 <40,000
04222
`Rweemraermkasd:e toVaadljuuesst froerptohretecdonacreenftorratthieonteosfttshaemtpelset.suNbostcaanlcceuliantitohnestest sample.
CONCLUSIONS
This testing indicated that some portion of the test sample biodegraded in
caearnonboitcuelnevioruotntmheentposssciabpialbtlyethoaftscuopnpdoirttiionngs
biodegradation. could be found
This study that would
ealxlcolwudfeortbhieopdoesgsriabdilaittiyotnhaotf athmeifclruooorrogcahenmiiscmalcocuolmdpeovneennttua,llnyorbecoeunlrdiicthed
that could metabolically alter this chemical.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
cRehlairaabcitleirtiyz:atiKoinimoifscthheratensktinsgub2.staThnicse spturuidtyylaancdksansauflfyitciiceanltmeasurement cofontecsltucdoenwcehnattraptoirotniso.nsBoefctaheusteesat smaimxtpulreewwearsetreesstpeod,nstihbeletefsotrintghecannot apparent biodegradation values.
REFERENCES
_
TLhaibosrsattuordyy,wSatsPcaounl,duMcNt,edLbaybtRheeq3uMesCtonmupmabneyr, PE3n0v2i5r.onmental
OTHER
Last changed: 5/24/00
C04223
3M ENVIRONMENTAL LABORATORY
DETERMINATION OF THE 28-DAY CARBONACEOUS BIOCHEMCIAL
OXYGE`ONECDDCEloMseAd NBolDte T(esCt,B#O3D01)0 (FMoOditRied) P3025
STUDY COMPLETED: March 28, 1987 FINAL REPORT COMPLETED: May 20, 1987
Env. Lab Request Number P3025
PNraempea:red by: Tie: Laboratory: Address:
S`AudsvaanncA.edBEenavcihronmental B3iMoloEgnivsitronmental LBuaiblodriantgor2y3-09 S9t35PaBulu,shMNAv5e5n1u4e4
C04224
2P8a-gDeay1 CBOD| May 20, 1997
In
this study
the
aerobic,
aquatic
ready
SUMMARY. biodegradability of P3025
in
general
accordance with
the
"Closed
Bottle
Test", OECD with removal
vMaeltuhesodofN<o.323%01aDnwda4s4%det(deurpmliinceadt.e)
The test obtained
safutbesrt2a8ncdeayws.as
found
to
be
not
readily
biodegradable,
OBJECTIVE
`Tehxepopsuurrpeousseinofg tahimsosdtiufdiyedwfaosrmtoofevOalEuCatDe Mtehethroedad3y01bioDd.egradability of P3025 after 5, 10, 20, and 28 days of
MATEARNDIMEATHLODSS
`TestSubstance: Appearance Aqueous Solubility
P3025, date 10/7/96, Lot 1 `Two-phase dispersion (clear liquid with opaque soli) Apparently not completely soluble in water at tested concentrations.
Available Physicochemical Properties.
BVoaiploirngPProeisnstu:re: Vapor Density: Evaporation Rate: Solubilty in water: `Specific Gravity: Percent Volatile: pH:
c1a0,018CmmH@g 20C <1 <1 Insoluble ca 12 60-70% 6-8
Date Received Storage. Test Solutions
Reported values
10116196
Inthe dark at ambient room temperature (22 + 2C)
Tsetsotckssoolluuttiioonnssw,eeraechprienpdaurpeldicaasteW,aotfearp-pArcoxciommamtoedlyat6e,d12F,ra2c5t,ioannsd(5W0AFmsg)./L wFoeurre
prepared overnight,
ininatehreatdeadrkm,inateraamlbniuetnritenrtomoemditae.mpeEraatcuhrew.asTcesatppseudbsatnadncaellwoawsed
to
mix
o`abpspearrevnetdlyinneotaccohmsptloectkelsyolmuitsicoinbalfetewritmhixwiantger.. ThAefcelweawrhiltiequpidarptoirctuiloantewsawsere.
`withdrawn for biodegradation testing.
`Tmheediraepaotrttheed bveagliunensiwngeroef tbhaesetodxiocnitynotemsit.nal concentrations in the test
C04225
2P8a-gDeay2 CBOD May 20, 1997
Test Organisms: Type Source Lot number Preparation
Polyseed-NX (with nitrification inhibitor)
Polybac Corp., Bethlehem, PA
A308 Cmoinnetreanltsmeodfiao.neSPoloultyisoneesdt-iNXrfocrrasipexstudylemipnluatceesd oinntoma5g0n0etmii.c amiexreart.ed Solution kept stirring while aliquots withdrawn to inoculate test bottles.
TestCongitions: Protoperiod TestVessels
Test Voume Loading
No. Repicates
Mineral Medum
24-hours dark 3S0ea0lemdLwictlheaarwgaltasesr sWehaelaatnodncBapOpDedb. ots with ground glass stoppers
300 mL b5lmanLksrewchoincsthitruetceediviendocnuolnuem added to each bottle except for media t2esetaccohncfeorntmraetdiionu.m bOlnalnky,osneeedreepdlibclaatnekf,orretfoexirceintcyechsetcakn.dard and each eParcephadriesdsowlivtehdHianc1h9 BitOerDs MNiultlriipeonrte MBiulfie-rQ Pwaitlelro.ws G(lcaastaslcoagrubeo#ys1a4e6r3a-t9ed8) whioutrhsl.aboCraartbooryyaoipefniilnegsd ctohvreourgehdawi0t.h2nuemwffioearmfoprlaupgpsraonxidmaaltleolwye2d-to stand overnight n the dark at ambient room temperalure.
Initial Stock Solution Loading-s nominal
6Fi7rstmRoeplllicate. 2162.39 mmgoilLl 51.6 mg
5D.6uplmigcaltLe 120493 mmggiiLL 542 mg/l
Test Bottles
fEraocmhttheestapBpOroDprbioatttleewwaalsers-eaecdceodmmaonddaftieldedfcraocmtpiolne.tely full with solution
Reference Standard
`CaGtl.uc#os1e4l8g6l5ut1a0m.ic aAcdiddemdix1tu.r5e.mLHoafcsholButOiDonsttoaendaacrhdrseofleurteionnceamsptualnedsa,rd 80D bottle
Toxicity Check
Abodtdtleedc1o.5ntamiLninofg HthaechtegstluscuobssetlaginucteamPi3c0a2c5idatmitxnteurheig1h0eastsleoeaddiendg.BOD
C4226
2P8a.g0eay3 CBOD May 20, 1997
Test Tep,C Stock Solutions pH Exposure Period Measurements
DDaattee TTeesstt TInetramtiendated Procedure
21 22C, continuously recorded. Initial pH values ranged from 6.5 10.6.7. 28days cDailsisborlavteedd boyxymgeeannasto0f,W5i,nk1l0e,r2t0i,atainodn 2o8f daaeyrsa.tedDiMsisloll-vQedwaotxeyrgeeancmhedtaeyr.
22182/8/9977 GTuhiedetlesitnegsenfeorraTlelsytifnoglloofwCehdetmhiecarlescommendations of the OECD
CALQULATIONS cCTaohlneccuerlneattpreoa,rttiieodpnovssastlieubseltseedtarhaeenbmdagtsheoefdccooanrrrbneoosnmpaiocnnaedloiunssgtobrciekoscsphoolenumstieicoadnlaltooaxadydeigrneignvsedadetmftrhaeonmbdetpgheierndnkuigpnigocfoaftteteshtdestsuetbsustdtyaa.rnecTeuhs(eeBdODt)o. The pmeurltciepnltyitnigodbeygroandeabhiulnldyreisd.hen cetermined by viding the CBOD by the Chemical Oxygen Demand (COD) and
RESULTS TPearbcleen1t.biodegradabilty values obtained ater5, 10, 20, and 28 days of exposure 1o P3025 are summarized in Ionxdyugpelnicdaetemdanmde.asurements, th percent biodegradability of P3025 was reported as <32% and 44% of chemical
ACCEPTABILITY CRITERIA
"This biodegradation test meets quality reria provided in test guidelines: 1) T`hseeegnliuncotshes-glalbuotraatmoircy.aciSdereefFeirgeunrcee1.standard 5-day C-B0D results were within the range normally 2) The oxygen depletion of the medium blanks was < 0.5 mg/L after S-days. 3) The oxygen depletion of the medium blanks was< 1.5 after 28-day. 4) TghreeatCe-rBtOhDanreishueltvfaolrutefhoeutnoxdifcoirtygcihueccokse(-hliguhleasmtitcesatceidd caolnoncee.ntrTahtiisoninpdliucsatgelducthoeset-egsltustuabmsitcaanccied)diwdansol innit the microorganisms. 5) Odnilsysotlhvoesdeodxaytgaenpocionnscewnhtrearteiotnheicotrhreebcottetdledwisassol>ve0d.5oxmygg/eLnwderroepuwsaesd f>o2c0aimcuoait.eaCn-d80thDe.final
o4227
28-Day CBOD Page 4 May 20, 1997
This test was conducted by Trudy K. Stiefenhoefer as requested by Pam Wolf of 3M Specialty Chemicals Division.
BEFERENCES
APHA, AWWA, WEF. 1995. Standard Methods for the Examination of Water and Wastewater. Method 5210 8, 5-Day BOD Test.
APHA, AWWA, WEF. Oxygen (Dissolved),
Az1i9d9e5.MoSdtifaincdaatriodn.Methods
for
the
Examination
of
Water
and
Wastewater.
Method 4500-O C.
OECD. 1993. `operation and
301 D Closed Development,
Bottle Paris,
FTresatn.ce.Guidelines
for
Testing
of
Chemicals.
Organisation for Economic Co-
OECD. Testing
o1f9C8h1.emiPcrainlcsi.plOesrgoafnGisoaotdioLnabfoorraEtcoornyoPmriacctCioceo,peArnanteixon2,aCn(d81D)ev3e0lo(pFimnealn)t:,7-P2a8r.is,OFEraCnDceG.uidelines
for
Polybac. 1997. Polyseed-NX, "The Original CBOD Seed Inoculum. Polybac Corporation, Bethlehem, PA.
C0428
2P8a-g0eay5 CBOD May 20, 1997
TABLE 1
SUMMARY OF READY BIODEGRADABILITY OF P3025
Exposure Period
5-Days Duplicate 10-Days Duplicate 20-Days Duplicate 28-Days Duplicate
cBOD, mg/Kg
<40,000 <40,000 <40,000 <40,000
<40,000 <40,000 55,039 <40,000
Percent of COD"
<32 <32 <2 <32
<3 <32 44 <32
*COD reported as 125,000 mg/kg
C0429
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C91231