Document RarbzzX6d6m74BRo5BQoo7VX7

AU9M.70*t ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE_______________________________________________ Identity: Mixture containing periluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-201F. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 1. The test sample is FC-201 F (F-11129). Current information indicates that FC-201 F is a mixture of 3.0% PFOS, 36.0% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7.0% sodium octyl sulfate, 6.0% suifonated alkyl ether sodium salts, 3.0% triethanolamine, and 0.15% tolyl triazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1992 Species: Daphnia magna Supplier: In-house cultures, maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, conductivity and DO Exposure period: 48-hours Test organism age: <24 hours old neonates Statistical method: EC50 calculated by Trimmed Spearman-Karber method. NOEC calculated using Dunnett's procedure. Test conditions: Dilution water: Shallow well water from Two Harbors, MN. Dilution water chemistry: Hardness: 170 mg/L (as CaCOa) Alkalinity: 147 mg/L (as CaCOa) pH: 8.61 Lighting: Cool-white fluorescent lights providing a daily photoperiod of 16 hours light and 8 hours dark. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by dissolving 1000 mg FC-201 F in one L of dilution water. Remaining concentrations prepared by serial dilutions of the stock solution. 000348 Exposure vessels: 250-mL borosilicate glass beakers containing 200 mL test solution. Number of replicates: four Number of daphnids per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: Conductivity range: (0-48 hours) 330 - 349 pmhos/cm (controls) 371 - 376 pmhos/cm (1,000 mg/L exposure) pH range (0-48 hours): 8.51 - 8.61 (controls) 8.47 - 8.48 (1,000 mg/L exposure) Temperature range (0-48 hours): 20.4 - 20.6 C (all exposures) Dissolved oxygen range (0-48 hours): 7.5 - 8.0 mg/L (controls) 7.1 - 8.0 mg/L (1,000 mg/L exposure) RESULTS_________________________________________________ Nominal concentrations: Blank control, 62.5,125, 250, 500, and 1000 mg/L Element values: 24-hour EC50 = >1,000 mg/L 48-hour EC50 = 308 (272 - 348) mg/L 48-hour NOEC = 125 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S __________________________________________________ The FC-201F 48-hour EC50 was determined to be 308 mg/L with a 95% confidence Interval of 272 to 348 mg/L. The 48-hour No Observed Effect Concentration (NOEC) was 125 mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________ ,_______________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and 000349 the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S ______;__________________________________________ This study was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number K1792,1992. O T H E R _______________________________________________________ Last changed: 6/26/00 000350 ASEcAnIAvSRiScreoIepnlcomSortrtucpIndoDyun#ltIiTDo0en1#s/5At5i-nD0Sg3Mc0ID--AD0iv.1Rui5sh3-io0iMt1nh STUDY TITLE ACUTE TOXICITY OF FC-201F TO DAPHNIA MAGNA DATA STANDARD OECD GUIDELINE 202 STUDY COMPLETED August 21, 1992 TESTING FACILITY AScI Corporation AScI-Duluth Environmental Testing Division 112 East Second Street Duluth, MN 55805 Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5030-015-01 3M Company Lab Request# K1792 Sponsor 3M Compsny Sponsor Study ID# K1792 1 000351 AScI corpontioo/AScI-Dululh ASEcnivRiroepnomrtenIDta#l T0e1s5ti-nDgMDAiv.Rii3ioMa AScI Study ID# i 030-015-01 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-201F to Daphnia magna OECD Guideline 202. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. K1792. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Dave Nessa Dinesh Vaishnav Donald Mount August 17-19, 1992. Dinesh Vaishnav Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Sponsor 3M Company Sponsor Study ID# K1792 2 000352 2.0 OBJECTIVE(S) ASEcAnIAvSRiSrcoeIcnpIcmoSorfettupnIdotDyn!#tIiToD0o1a#/5tAm5-D0Sf3Mc0ID--AD0iv.1Rui5sl3-iuo0Mdn1l To determine the 48-h EC50 and 48-h NOEC (no observed effect concentration) of the test substance, FC-201F, for Daphnia magna. This study was conducted according to the AScI study plan I 3.0 TEST METHODSretained by the Sponsor and OECD Guideline 202 (OECD 1984). Test Substance Test Organisms Dilution Water Properties: FC-201F, orange/red liquid, relative density 1.1 (water = 1), stable, and miscible with water. Test concentrations: 0.0 (dilution water control), 62.5, 125, 250, 500, and 1,000 mg/L, prepared by a serial dilution of 1,000 mg/L stock solution. Throughout the test, all test solutions appeared clear with a persistent foam at the surface. Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScI stock culture. Use in test: Gravid.adults isolated in dilution water 24 h before the test initiation. Used 5 organisms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. Source: Shallow well water from the Two Harbors, Minnesota area. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, a hardness of 170 mg/L (as CaC03) and a pH of 8.61. Aerated for 24 h prior to using in the test. Sponsor 3M Company Sponsor Study ID# K1792 3 000353 ASEcAnIAvSRiSrcoeIcnpIcmooSrrtetupnIdoDtyra!#tITiD0oe1o#s5Mt5-inD0Sg3Mc0ID--AD0iv.1Rui5sh3-io0iMt1nh Exposure Chambers Incubation Observations Endpoint Calculations Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observa tions were made. Duration: 48 h. Daily photoperiodz 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20.4-20.6C. Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1) At test initiationtotal hardness and alkalinity, (2) dailytemperature, and (3) at test initiation and termination- DO, pH, and specific conductivity. All determinations were made according to APHA methods (1989). EC50: Trimmed Spearman-Karber method (Hamilton et. al. 1977). NOEC: Dunnett's procedure (TOXSTAT stati stical software, Version 3.1, University of Wyoming 1989). Sponsor 3M Company Sponsor Study ID# K1792 4 000354 3.0 RESULTS ASEcAaIAvSRSicrecIapIucooSnrittepuaoIdDuryml#ItTDi0oe1#orJ/t-5AiDn0S3Mjc0DI-A-0Div.1Ru5iil3-iu0cMto1h Test Substance Toxicity 24-h EC50: >1000 mg/L. (Pertinent data are in Table 1) 48-h EC5 0 : 308 mg/L. 48-h NOEC: 125 mg/L. Ranges of Water Chemistry Parameters Total hardness (as CaC03 mg/L): 170-186. Alkalinity (as CaC03 mg/L) : 147-152. Temperature (C) : 20.4-20.6C DO (mg/L): 7.1-8.2. pH: 8.47-8.64. Specific conductivity (umhos/cm): 330-372. 4.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity test (this test is optional). Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 80% of the air saturation value at 20.4C. The test duration was 48 h. Final report for the most recent test with sodium chloride is in Raw Data Package. Spocuor 3M Company Sponsor Study ID# K1792 5 000355 5.0 REFERENCES EAnvSircoIncmorepnotsrs!tTioeos/tAinSgcDI-Divuisliuotnh AScIARSecpIoSrttuIdDy#ID01#5J-D03M0-A01.R53-0M1 American Public Health Association (APHA). 1989. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Hamilton M.A., R.C. Russo and R.V. Thurston. 1977. Trimmed Spearman-Karber method for estimating median lethal concentration in toxicity bioassays. Environ. Sci. Technol. 11:714--719. Organization for Economic Cooperation and -Development (OECD). 1981/1984. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. University of Wyoming. 1989. Toxstat Version 3.1. Fish Physiology and Toxicology Laboratory, Department of Zoology and Physiology. University of Wyoming, Laramie, W Y ._______ Sponsor 3M Company Sponsor Study ID# K1792 6 000356 ASEcAnIAvSRiSrcoecIpnIcmooSrrtetpunIodDtyraal#tIiTD0oe1n#r5/tA5i-nD0Sg3Mc0ID--A0Div1.Rui5al3-iu0oMt1nh Table 1. Acute toxicity of FC-201F to D. magna Test substance nominal concn (mg/L) 0.0 62.5 125 250 500 1000 Cumulative number and % of dead and immobilized organisms* 24 h 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 4 (20) 48 h 0 (0) 0 (0) 0 (0) 4 (20) 20 (100) 20 (100) `Percentages are in parenthesis. EC50 and NOEC 24-h EC50 48-h EC50 48-h NOEC Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L)b >1000 mg/L 308 mg/L 125 mg/L 95% Confidence interval Not reliable 271.89-348.42 Not applicable I | bEC50 values were calculated based on the test substance nominal concentration. SSppoonnssoorrS3tuMdyCIoDm#paKn1y792 7 000357