Document RarB9Kkev69woEo3ayqy0baqE

M .6a a - 0216 ACUTE TO XICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not given. The test sample is FC-206CE (5/23/91). It's purity was not sufficiently characterized, though current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: OECD Method 202 Type: Acute static GLP: No Y ear com pleted: 1991 Species: Daphnia magna Supplier: In-house cultures. A nalytical m onitoring: Temperature, pH, conductivity, and DO Exposure period: 48-hours Test organism age: <24 hours old S tatistical method: Not applicable. Survival was greater than 50% at all test concentrations. T est conditions: Dilution w ater: Filtered natural well water Dilution w ater chem istry: C o n d u c tiv ity : 860 pmhos/cm pH: 7.6 Lighting: A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 20 uEs`1m`2. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in dilution water. Aliquots were then added directly to dilution water in test vessels to create test solutions. Exposure vessels: 250 mL Pyrex glass beakers containing 200 mL of test solution and a water depth of ~6 cm. Num ber o f replicates: 4 Num ber o f daphnids per replicate: 5 Num ber o f concentrations: Five plus a blank control 001785 W ater chem istry during the study: Conductivity range (0-48 hours): 860 - 880 |jmhos/cm (control exposure) 860 - 880 pmhos/cm (1000 mg/L exposure) pH range (0-48 hours): 7.6 - 8.3 (control exposure) 7.7 - 8.3 (1000 mg/L exposure) Tem perature range (0-48 hours): 20 - 21 C 19.0 - 20.2 C (control exposure) 19.0 - 20.1 C (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.5 - 9.2 mg/L (control exposure) 8.7 - 9.3 mg/L (1000 mg/L exposure) R E S U L T S ________________________________________________________ Nom inal concentrations: Bk control, 150, 250, 400, 600, and 1000 mg/L Elem ent values: 48-hour EC50 = >1,000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________ ___________ The FC-206CE 48-hour EC50 was determined to be >1000 mg/L, the highest concentration tested. No mortality or sublethal effects were observed at this concentration. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ ' Test was conducted by EnviroSystems Division or Resource Analyst, Incorporated, Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-2, 1991. 001/ OTHER Last changed: 6/26/00 001787 0017S8 1 .Tv'-:-* *;. 4~ %: BEST COPY AVAILABLE I. summ -`4. . The acute toxicity of FC-206CE to the daphnid, Sapboia magna, is described in this final report. The test was conducted for 3H Company for' 48 hours during June 10 to 12, 1991, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Jeanne Nagazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Vard. . The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 1C. The dilution water"was filtered natural well water collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of PC-206CE were: 0 mg/L (control), 150 mg/L, 250 mg/L, 400 mg/L, 600 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average vet weight (blotted dry) of 0.0006 g, resulting in a loading rate of 0.015 g/L. All daphnids were in good condition at the beginning of the study. The test substance was not toxic to daphnids at tested concentrations. Exposure of daphnids to the test substance resulted in a 48 hour EC50 greater than 1,000 mg/L FC-206CE. fe ` i i. I M }. n>*,> , , i-yV! m ft** 'V.- O j M Jr . 4 if5. Vi?1 ,V; ;yv' .ii sr |Page'2fof 14, 'kf. S '"Mix MM Mi* m ml, 4$ToiMiUJPORE t b e s t-c o p y a v a il a b l e i n . IKBEX OP TABLES M B PICURBS Table 1. Cheaical characterization of a representative saaple of natural veil water used as dilution water for toxicity test Table 2. Survival data froe toxicity test , Table 3. Median effective concentrations (EC50s) froa toxicity test Table A.l. Conductivity, pH, teaperature, and dissolved oxygen concentration aeasured during toxicity test PACE 6 9 10 13 b e s t c o p y a v a il a b l e IV. INTRODUCTION This study was sponsored by 3M Cospany, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC-206CE to tbe daphnid, a freshwater invertebrate. The report contains sections that describe the eethods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. - V. METHODS AMD MATERIALS TEST SUBSTANCE: FC-206CE (EnviroSystems Sample Number 2936E) was delivered to EnviroSystems on May 23, 1991. It was contained in a 4 oz. plastic bottle that was labelled with the following information: "J1884-2, FC206CE". FC-20SCE (a yellow liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be archived at EnviroSystems for a minimum of 10 years. DILUTION HATER: Hater used for acclimation of test organisms and for all toxicity testing was collected from wells at EnviroSystems in Hampton, New Hampshire. Hater was stored in 500-gallon polyethylene tanks where it was aerated. Results of chemical analysis of a representative sample of water are presented in Table l.1 TEST ORGANISM: Juvenile , daphnids employed as te s t organisms were from a sin g le | source and. were id e n tifie d using an appropriate taxonomic key. Daphnids /.u se d in the test,w ere produced from an in-bouse culture and were le s s , than {'_24'^hours o ld ia t^ th e v s ta rt.o f the t e s t . Control daphnids were weighed at "the y conclusion of ;= th ei t o x i c i t y - t e s t . . P rior to te s tin g , daphnids were maintained ' in 100% 'dilution.waterunder s ta tic .conditions in 4 l i t e r glass S5'Ja rs."$%During > acclinationfdaphnids.-were hotj. trea ted fo r d is e a s e .and they lyw ere.yfreev of apparent^ h i c k n e s s . ' i i i n j a n d "abnormalities a t $ the beginning/of the ^ tiss^ ^ ^ D u rin g the acclimation period 24 hours p rio r to th e ^ te stV in itia tio n fth 1 > 4 te n ^ ra .U re was 19.5?C. Daphnids were fed y east, P ^ tro u ty ch o w ,' and/or^theUfresliw;ater,ialg*3fdHlnstrum ctp rico ro utum once or.tw ice'daily beforey.thr t e s t ^ ^ l p M ^ i 'i y l i y . v f. V. S-;M t>S,V ~ J fidili', V tefs fOUMUPOK > oor ?1 _&'*!>* fT'T ''VW* .t,- `. ' r-v s. tJVv - ? ','. II V..- ?- ,, .5';, ;' v > ^ -;/;.7 .-.v. a.*VWjJx-v'-. r`v. - BEST COPY AVAILABLE Table 1. Conical characterization of a representative staple of natural veil water used as dilution water for toxicity test Paraaeter Unit of Neasureaent Reporting Liait Measured Value PH pH units -- 7.6 Conductivity uahos/ca -- 860 Organocblorine ug/L 0.5 MU pesticides Organopbospborus ug/L 0.5 HD pesticides Polychlorinated . ug/L 0.5 ND biphenyls . ; > 1 . V, ^ *3Sfeif'''.'W1* -4 Hsotres: 1i..yv<ijoneXietected at;or-above the reporting liait. - ------ dilution water ^ S a S ^ Y ; *.'*1[eUi^^irm/ftaotroZ.test vessel prior to ; feu. ;,,introductionfoivorganisas'and pesticide and PCB data is ; ^liTCtedMurinroutin,biannual water quality testing.: ..................... - <&$ L ... ........ vs* Mi pr fV i-y 001793 i.V V .-l. V *4^*. - ' .* * ' ' . '-'r>>T< ,y ^ - BEST COPY AVAILABLE TOXICITY TESTING: The definitive toxicity test wee performed during June 10 to 12, 1991. It is besed on procedures of the OECD (1984). The test Was conducted at a target tesperature of 20 t 1C with five concentrations of test substance and a dilution water control. X 1,000 mg/L stock solution was formulated without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control), 150 ag/L, 250 mg/L, 400 mg/L, 600 ag/L, and 1,000 ag/L. Twenty daphnids were randomly and equally distributed among four replicates of each treatment. The test was performed in 250 ml glass beakers that contained 200 ml of test solution (water depth was approximately 6 cm). Test vessels were randomly arranged in an incubator during the 48 hour test (a random numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 20 uEs^m-*. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The number of surviving organisms and the occurrence of immobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 48 hours. Dissolved oxygen (TSI Hodel 57 meter; instrument number PRL-3), pH (Beckman model pHI 12 meter; instrument number PRL-51), conductivity (Labcomp SCT meter, instrument number PRL-50), and temperature (ASTH mercury A thermometer; thermometer number 2265) were measured and recorded daily in each test chamber that contained live animals. \ ' STATISTICAL METHODS: , - f t * -V-' i ^Results, of .the`^ t o x i c i t y , test could not be 'interpreted by tahdard.'statistical techniques..(Stephan; 1983) because 50% survival or 'greater occurred in all tested^concentrations. y,,, <w * *-*<*t'ii > fe t M s b ' f.;/; M, OOl ?:>iv'"'-&JMij :%*: **v *. - ^ %V '/&%*,* ^?' l\/ * *.. L v , ^ :> ft `< r ^'e*^?x r... - ...- 1/ 7 fi Table 3. . Median effective concentrationsf<K50a) from toxicity teat Exposure period EC50 95 percent confidence Uni t s Calculation aethod 24 hours >1,000 sg/L 48 hours >1,000 sg/L -- <7- - ' * ;v"l - j ; y. -- -- B IS T COPY AVAILABLE X *& "> w; p - fv . ft* f'-Cii' -A' oolvgv BTr *i>. I*.-- gr;,, .s,/;-: * ' `"%$:1<-r v vs ra. um ncu OECD. 1984. Guideline* for Testing of Cbenicals. Section 2: Effects on Biotic Systems. Method 202, Daphnid >pf Acute Inaobilization Test and Reproduction Test* Adopted April 4, 1984. BEST COPY AVAILABLE y* 'i ,,'y/. \\ * ' ' - '" * *1 -v \ , A Y , , , *:< ' ~vi -, ' Imi-I S|jSaB|H "H a ^ -'-' i ;:~ * ^.r- . S.4 Tz&ii'm ;*. 'i ^ l' ' ' 1-Y --' .^ry-t-f^J >' '1' >` , \ ? - 4**.r V,1*;- / '.y^-l - x-;...y.$. / r-'\ FVV'i-f&J,-.I :iy. 1 I'-t'S-'V; * -e >' V . ^ * tY.^wV . f . V-.'/i. <-v , Sfe& I -<ds k'iv 001798 Iay 'f COPY AVAILABLE Tabl A.l. Conductirity, pH, taiparatura, aod difiolTed oxygen concentration aeasured during toxicity tat / V1' V "Vi - i. f . - ' Hoainal concentration (*g/U tap. Conductirity (uaho/ca) 0 24 48 hr hr hr ?- PH V' > ` ` S'* 0 (control) 1' " 2 3 4 860 860 860 860 150 1 860 2 860 3 860 4 860 250 1 870 2 870 3 870 4 870 .? V . 400 h f# c -: hI i'Sr S \ ", :c f 600 1 . . 870 2 , 870 3 > W 870 4 ' \\ 870 870 880 870 880 870 880 870 880 ; 860 900 860 900 860 900 860 900 870 870 870 870 870 870 870 870 870 870 870 870 r. 860:f 860;* / 860 f '. 87o,, , .860:1 ,870;,.. 860; HVtO'MX' 860 7.6. . 7.7 7.6 7.7 7.6 7.7 4: 7.6 m - . 7.7 'I# '. t'-S ; 7 .7 ^ |y , 7.7 , 7.7v ^ , j 7.7 % >7^te:7.7 LT rX*J,l; f Ci i `I i-*;! -' S ' -.0: % ' A. ' `` * J. v > S r- VflIMfw o o is <