Document Rao0ZK4wbwQOND3Nr9d0jaeyn

ar &A6- 0 7 og ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-201F-X. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 1. The test sample is FC201F-X (F -11129). Current information indicates it is a mixture 3% PFOS, 36% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7% sodium octyl sulfate, 6% sulfonated alkyl ether salts, 3% triethanolamine, and 0.15% toluoltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemicai component o f the test sample. M E TH O D _________________________________________________________ Method: OECD Guideline 209 GLP: No Year Com pleted: 1992 Analytical m onitoring: Dissolved oxygen concentrations, temperature. Statistical m ethods: Not applicable. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution w ater: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A 500 mg/L stock solution of the reference substance, 3,5-dichlorophenol, was prepared. The pH of the reference solution was measured to be 7.8. Test substance was added directly to the test vessels. Test vessels: Not given Num ber o f concentrations: one plus blank controls, all in duplicate Tem perature: 17.7 - 19.0 C Total suspended solids and pH on day o f testing: 3.4 g/L (TSS in final: 1.36 g/L. Initial pH 7.5 and final pH 7.5. Elem ent Basis: Respiration inhibition as determined by oxygen consumption. Rem arks: Uptake rates corrected to 20C for calculations. RESULTS________________________________________________________ Nom inal concentrations: Blank controls and 1000 mg/L, all in duplicate Elem ent values: 30-minute EC50 = >1000 mg/L 000400 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ _ The test substance 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ R eliability: Klimisch ranking 3. Several pages are missing from this study. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number K1126-1,1992. O T H E R ___________________________________________________________ Last changed: 6/26/00 000401 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED UQUOR LR K ) l 2 L - i Date _ 2 > n \ t o Z Analyst K&n Page _L_ of "7 Type Test Q ^ - C D / \ < - T m t o S lu o b i R ^sP ut^noN X */ i3 rm N T zsr *2 0 ) ACTIVATED SLUDGE MIXED LIQUOR Date Obtained 3 / 2 3 / g) 2 Date Used 3 / 2 3 h z Mixed Liquor Suspended Solids IMLSS) 3 . ^ g/L Adjusted to - g/L MLSS in Anal test solution g/L Initial pH ~7.5~ (1st test soln. prepared) Final pH "7 -5 " (last soln. prepared) 'Comments _____________________________ : ___________________________________ TEST SUBSTANCE Sample Description V-C- 20 l ______ V --) ) ) Lot I____________ Initial pH of highest cone, tested ( loco Adjusted to pH ______ Comments Lta~r>______ _____ _____________________________________ BEESBSNCg SUBSTANCE Sample Description _____ 3 . ^ - ^cH tjortcPH gN ci___________________________ Initial pH of stock solution ( 5~oC mg/L) ~7-1T Adjusted to pH ~~ ~ Comments _________ ^ ____________________________ ;____________________________ EXPOSURE CONDITIONS Test Duration _______ 3 ) YAc-j^ _____ _________________________________________ Test Temperature Range: IT.") ~1^-0 C ------- Comments _______________________________________________________________ _ 000-G02 ENVIRONMENTAL LABORATORY LR 5 cf- 1 OSCO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION Test Substance: Sample Description: Analyst: Date: V .9 N * C~ \ \ ----- X __________~ \ \ \ ^ ) L O T 1 ____________________ ;_____________ Test Cone. mg added `ma/L oer 500 mL ^ L -L cHTHOL mL MOO ) water mL syn. sewage mL microbial feed oer 500 mL inoculum Der 500 mL Total Volume mL ______a s a ____ _______ \U ____________________________ ______ 5700___ YOCO-A -COO ______2 S 4 _____ \b 500 5 00 \o c o -(S _____ S o o ___ ______ oU_vqwS *___________ 1 vocO - A - ^ 7 ______________________ \OOQ -fr> ~ *7>~) _______ a o Q __________ ______ ~00__ (1) Millipore Milli-Q*^ high purity water 000403 ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS Test Substance: FC - 201F-X Analyst: KSN Lr: K1126:1 Date: 2 /2 3 / 9 2 mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp, "C R (D Ra (2) % Inh. (3) %\ * % v s BK1 8.7 8.5 8.1 7.8 7.5 7.2 6.9 6.6 6.2 5.9 5.5 17.7 0.6473 0.7595 - 1000.00 2.7 2.4 1.9 1.4 0.9 0.4 - -- - - 18.5 1.0000 1.1099 0 1000.00 4.1 3.6 3.3 2.8 2.4 1.8 1.4 0.9 0.5 - - 18.6 0.9000 0.9920 0 BK2 8.3 7.9 7.7 7.4 7.0 6.7 6.4 6.1 5.7 5.3 5.0 % diff in controls (4): 0.5 EC50 (95% Cl): >1000 ma/L 17.9 0.6594 0.7631 - RaCm (5): 0.7613 3-Hour Exposure ______ ______ ______ Dissolved Oxygen Concentrations, mg/L mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 .. 5..x5.x... Temp, "C _ R ,( i) Ra (2) % Inh. (3) BK1 7.9 7.7 7.4 7.1 6.8 6.4 6.1 5.7 5.4 5.1 4.8 1000.00 8.3 8.0 7.6 7.3 6.8 6.3 5.9 5.4 5.1 4.7 4.3 1000.00 8.8 8.5 8.1 7.9 7.5 7.2 6.9 6.5 6.1 5.7 5.4 18.1 19.0 18.1 0.6582 0.8388 0.6909 0.7511 0.8992 0.7885 - 0 0 000404 BK2 8.6 8.4 8.1 7.7 7.4 7.1 6.7 6.5 6.1 5.8 5.3 % diff in controls (4): 0.8 EC 50 (95% Cl): >1000 ma/L 18.2 0.6679 0.7569 - RaCm (5): 0.7540 (1) R= Oxygen uptake rate (mg 02/L-min) (2) Ra= R adjusted uj 20C= [R/e.Exj (3) % Inhibition- ((RaCm)-RaS/(RaCm)]100 (4) % dHterence in controls: [IRaBk1-RaBk2l/RaCm]100 (Two controls must be within 15% o( each other.) Idbmn adliifited R controlfin [RaBkURaBk2l/2 0 * 1.072 R aC tW mean adjusted R, controls Bk= blank control, S * sample Tobss sample temp x - Tobs-20*C