Document Rak9b76Jd8Mnpn58Qdmk9J6b8

FOR DU PONT USE ONLY AR226-2938 Du Pont HLR 55-89 Study Title Statib Acute 96-Hour 1050* of to Fathead! MInnow^PlinephaTes promeTas) Author. David G. Hutton Study Completed On February 20, 1985 Performing Laboratory E. I. du Pont de Nemours and Company, Inc. Haskell Laboratory br Toxicology and Industrial Medicine Elktion Road, P. 0. Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 55-89 Page 1 of 9 company SanRized. Does TI(A contain TSGA CB8 Ou Pont HLR 55-89 GOOD LABORATPORARCYTICE STATEMENT This study was conducted;accord Ing to EPA 606d Laboratory Practice Regulations (40 CFR 792). Anfr areas of noncompMance are documented In the study records No deviations'existed that significantly affected the I validity of the study. ' Submitted: E. I. du Pont de Nemours and Company, Inc. Sponsor?: \ Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Study Director*: ^4^^ ^ ^^n^L 0!v1d G. Hutton Consultant Environmental Sciences A / f l7tf 2 - Compaq Sariui^ed. Does not contain TSCA CBE GENERAL INFORMATION Du Pont HLR 55-89 Material Tested: Medical Research Ho.; h'askell No.; Purity: Synonyms; 17.503 Submitter's Notebook Number: Sponsor: Material Submitted By: Study Initiated - Completed: Notebooks; There are 9 pages in this report Distribution: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Wilmlngton, Delaware Inc. Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Jackson Laboratory Inc. Test initiated: Test completed: Study completed: 1/10/89 1/14/89 1/26/89 D 3 - company Sanitized. Does not contain TSCA CBi Ou Pont HLR 55-89 Title of Report Sthtl^cute 9g_HourLCSO8of to Fathfead M1nnows(P1iephaTespromeTas) Suinmary (H-17,503) exhibited no acute toxiclty to fathead minnows, a warm freshwater fish, under static unaerated test cond1^1onsjiur1rKi^J)6-hour exposure period. The 96-hour LC50 was >1000 mg/Lc because there were no mortal 11:1es' at nominal test-concentrattons-ap to-and- Including 1000 mg/L. The solubility of ------------iMW--------r (H-17,503) was found to be less than 360 mg/L, as there was undissolved material tn all test concentrations. Reported by: James U. Wetzel Technticlan / - '.{}. ^i,<'//_- ^ Mo/Day/yr Study Director; i. ^^>-^^ David ;G. Mutton ^). ^-^^r^. Consultant Environmental Sciences :zA/^ Mo/Day/yr Approved by: -?A.^.l^ Frank X. Phllllps.;Ph.D. ^-Zf^^ Mo/Day/Yr .cect1(in Supervisor' EnvironmentaT Toxicology and Information Services Reviewed and Approved for Issue: ^.LiL A David'G. Hutton Study Director ^t^vv //w<^ Mo/Day/Yr JWy/OSH/FXP/smk/AQRP28.4 4 - Company Sanitized. Does not contain TSCA CBI Du Font HLR 55-89 ' QUALITY ASSURANCE DOCUMENTATION Static Acute 96-Hour LC50 of to Fathead Hinnovs (Pimephales promelas) Audit Dates 1/6/89 ^ W SHORT-TERM AUDIT REPORT NUMBER;! DATE FINDINGS REPORTED TO MANAGEMENTAND STUDY DIRECTOR; 1/6/89 Reported by: ^W^/- g^T^----- William J. Lynam Quality Assurance Auditor ^//t/8^ Date ^^any Sanitized. Does not'contai-n TSCA CBt DU Pont HLR 55-89 Introduction/Purpose To det ------ ( ermine H-17,50 th 3) e t s o t atic fathe ac ad ute'toxi minnows c , l ty a o f warm _fr_es_h _wa_te_r _fi_sh_. Materials/Methods (Aquatic SQ('-T02) prepared as a l^'ing/Sntock^oTutlor^nHaskelTLaboratory well water, placed Into 5-liter aquaria, and diluted with laboratory well water to yield the desired nominal exposure concentrations In 4 liter final volumes (12-cm liquid depth). Two 1denticalivessels containing only laboratory well water were designated as the H^Ocohtrols. Chemical stability of the test material In well water was not determined. Ten juvenile, unsexed fathead; ml nnows (Pimephales promelas) from Hasken-bred stock with a 1.6 cm mean standard length (range 1.2 to 1.8 cm) and 0.046 g mean wet weight (range 0.015 to 0.078 g) were randomly placed In the test vessels, two test vessels per' concentration. Loading was 0.12 g ftsh/L. Fathead minnows are Identifier by labeling the culture and test vessels. The fish were maintained at Haskejn Laboratory for approximately 163 days, with the last 110 days In a 272-liter Fiberglas tank (84-cm in diameter by about 49-cm liquid depth) before being used for this test. Holding temperature was within 3C of testing temperature throughout the holding period. The dissolved oxygen concentration during acclimatization was assumed to be at or near saturation because air was continuously bubbled through the water. No treatment of the fish for diseases was required during the holding period. Fish were not fed for approximately 72 hours prior to nor during the exposure. The test solutions! were not aerated, and temperature was maintained between 21.7C an4 22.6C. Photoperiod was maintained at 16 hours 11ght:8 hours dark. Mortality counts and observations were made approximately every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were .measured in the IU) control and in the low, medium, and high test concentrations at the beginning of the test and at approximately 24-hour intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA), and conductivity were measured at the beginning of the test in the HnO control. Records All records from this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Del aware) or archived at the Ou Pont Records Management Center (Wilniington, Delaware). (Company Sanitized. Does not contain TSCA C81 Du Pont HLR 65-89 Results results for fathead minnows exposed to '(H-17,503) are presented In Table 1. our LC50 was >1000 mg/ because there were no mortalities at nominal test concentrations up to and Including 1000 mg/L. All test solutions (from 360 to 1000 mg/L) were milky with undlssolved test material floating on the surface and settling to the bottom. The chemical and physical parameters measured during the test of |R acceptable ranges for this itudy. D1scussion/Cond usion __ has been tested for acute toxiclty with fathead minnows, a warm fre' water fish. Under the conditions of this test, ------t wa^ found to exhibit no acute toxiclty at test concentrations abov^ Its solubility limit In water. A preliminary rangeflnding test on this compound was run but data are not reported here. In this test} all the fish survived at dose levels of 1000, 100, 10, 1, and 0.1 mg/L, plus a HgOcontrol. Footnote ^CSO = That concentration which 1s lethal to 50X of a test population during the specified time period. D company Sanitized. Does not contain TSCA CBi Ou Pont HLR 55-89 TABLE 1 RESULTS Op A 961-HBUSTRATIC ASMTE T9XICITY TEST WITH FATHEADMINNOKS EXPOSEDTOJ1 fH-17.503) Nominal Test Concentrations .-(nig/U- H^OControl 360 600 1000 Cumulatlve Observed -'24-Hr. -48 -HFi- At 6* 'A* fiV- Mortal An* - ,^Hy (1i} -% -Kf~ TP"~ Mr. fe*" 1 0 0 0 0 0 0 0 0 do 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 *Rep11cate exposure chambers containing ten fish 1n each. 8 - Csmpany Sanitized. Does not contain TSCA C8l Du Pont HLR 55-89 RESULTS OF PHrSICAL AND CHEMICAL PARAMETERS MEASUREDDURING A |96-HOURSTATIC ACUTETOXICITy TEj FATHEAD MIHHQUS EXPOSED TO|;~ Physical/Chemical Parameters NomlinalTest Concentrations ----1885----------go5-------- (HUgh) (Medium) Dissolved Oxygen (mg/L) 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 8.6 8.7 8.7 7,6 7.7 7.7 6k8 7.1 7.3 6}. 5 6.9 7.1 6^9 7.1 7.5 H 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 7^6 7.6 7.6 7+1 7.1 7.1 7,4 7.4 7.4 7+3 7.3 7.3 7*4 7.4 7.5 HgO Control 8.5 8.0 7.7 7.5 7.8 7.6 7.5 7.7 7.6 7.fi Water Chemistry on the HoO Control at 0 Hours Total Alkalinity (mg/L as CaCOj) EDTA Hardness (nig/I, as CaCO-J 90 70 Conductivity Ojmhos/cm) 150 D ; .mpany Sanitized. Does not contain TSCA CBB