Document Raa1VybGYG2L0QyOX9wnk4v8V

am Lest 7/ 2(/ AR 226 _ 350 SHENVIRONM`ENPTARL LONASOJOERE0A5TC0ORT2Y Analytical Report Coma. ~ Cay Definitive Study: Quantitative Screening of Commercial Lots of Human Serum Obtained in 2004 for Endogenous Fluorochemicals Using Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry LaboratoryRequest Number: 05-0442 Testing Laboratory 3M Environmental Health and Safety Operations 3M Environmental Laboratory - eee 8 3M Environmental Laboratory 5 UR MaPrrkojEe.ctEfLleeafdson BPuiOldBinogx23-333E3-109 Saint Paul, MN 55133-3331 Phone: 651-778-5405 2 z 3 g=z m= s Te`sWtiilnigamFaKc.ilRiteyaMgaenn,agPehm.De.nt BPuiOldBinogx23-333E3-109 Saint Paul, MN 55133-3331 Phone: 651-778-6565 Requestor BuiDladliengB2a-co3n-0 PO Box 33331 SaintPaul, MN 55133-3331 Phone: 651-778-4736 Page tof1o ----------------ee-- e ---- ee-- e SEWNVIRONMEPNRTAOLILNEAOBCOER0TA8T0O4R2Y 3M Environmental Laboratory Analytical Report Project Number: E05-0442 1 Introduction / Summary `The following analytical report presents data from commercially available normal `pooled human serum that were purchased by the 3M Environmental Laboratory and. subsequently screened for endogenous fluorochemicals. `Thiny-six separate lotsofpooled normal human serum were extracted using a solid `phase extraction technique and scresaed for sixteen different fluorochemicals using liquid chromatographyandemn mass spectrometry (LC/MS/MS). Serum samples. were received from various suppliers between9/9/04and 12/2/04. Sampleswere extracted on 5/11/2008 and analyzed 5/23/2005. Heptafluorobutyric acid (PFBA) was includedinthe original lstofsixtoen target analytes. However, duc to instability in the methanol extract, analytical data for PFBAwillnotbe included in this report. Instead, aseparatestudywillbe conducted including the re-extractionofthe thirty-six lots ofcommercial serum with acetonitrile and analysis using a newly developed method that provides isomer resolution. Those results will be reported in a separate report. `SampleresultsaresummariniTazbleesd1and2. Theavevarlueasregporeted represent samples in which there was detectable analyte. Sample values that were. less than the limit ofquantitation were not included in the average value calculations. Please refer to Section 5 for individual sample results. Page2019 OT oso: Si. Son ESR eon rn [ro rox|con [ron [weone [ool[oSon o ounfomt [omn [[ oom [owa e[|oma] Er [Medien |105 [owes] ate|a21 |orwr |oa |014[cow| [ [n tiogw h Tzouur[[oommean ooamns:[|sorme oimeer||oosoe|[ooort[oaooen| [StandardDeviation |0378 [oooe22|ait| 127 ows|ors |ors|oous| Detectable Analyte. 1 Tai2. Summaofteeanpeed nd HoyFated ArtRests Sr [Meawn eoorn T|ewomaaT[eoeuwnrs[[oooooowmms[[[o2emsw[||oswesww |weeo |ouommm||] Sartrowouster |emma|[ocoomm |soomve[oovmor||somw ||wi|oouvrrs|| Number of Samples with Detectable Analyte NA" =NotApplicablebecausethisanalytewasonlydetected in oneof thesamples. INA? =NotApplicablebecause thisanalytewas notdetectedinanyofthesamples. brn wenoST yosers 2 i. rnc Stren Reference Substances arene we [rdro [mE mN s[mAeonm| | wemr [orsomron|wor[| oerercmto]r rPeotttiss ||ovninrcein||Gireeemroms|| wtoawn |orm ||ooomr]] [sreetmostmicied || tOoresni||ctoowr ||romonoamnan| iow| om] Const | owmeoarins| Ttonre weonre | oomm retniodenaicaid | _ CizAcd ree wen |om| Tetum | oA ||W Tonieo0imosn wine| owe Perfluorooctanesulfonwmide | FOSA 1 rs Someone va wm TCR-280(99131-038)| 98.94% Nome TM Rese | men oon om [oe] BpESae TE T E|mew | ween n | S|| Perfluorooctano acid, PrOA [12% : in z . NiMsoRmerre,saunldttsihnediPcFaOteAtrheaftetrheencPeFsOuSbsrteafnecreencconessiusbtsstoafnc2e2c.o1n%sibsrtasnocfh3e0d.i8s%omberra.nched Thesevalues wereusedforthedetermination ofthe isomerratiosintheserum samples (presented in Table 10). . WMENVIRONMR ENTALO LWAOBCER0C A6bTOtRY 3 Sample Preparation "The samples fortis study were prepared as follows: 1. Theserum samples wereallowed tothawandwarmtoroom temperature. 2. Four millilitersofserum (or water in the caseof method blanks) were added to 15 mL screw-capped polypropylene centrifuge tube. 3. Matrixspikesandmethodblanks werepreparedby spikingtheserum(or water) with the analytesof interest. In addition, ''C PFOA and *0; PFOS `wereadded assurrogatestoallserumsamples,calibrationstandards,matrix `blanks, and method blanks at concentrations of 1.04 and 0.950 ng/mL serum respectively o evaluate extraction method performance. 4. Ten millilitersofasedoniile were added to the serum and the centrifuge tubes were capped and vortex mixed, allowing the serum proteins to precipitate. 5. The tubes were centrifuged at approximately 3500 rpm for 20 minutes to pellet the precipitate. 6. After removalfromthecentrifuge,thesupermatantwastransferred10 abottle containing 70 mLofwater. 7. The samples were passed through preconditioned SPE cartridge and cluted with 2 mL ofmethanol. `Quantitation was accomplished using high performance liquid chromatography tandem mass spectrometry. The following parameters were used: Liquid Chromatograph: Hewlett-Packard Series 1100 Liquid Chromatograph sAynasltyetmical column: Keystone BetasilTM Cis 2.1x100mm, Susparticle size: `Column temperature: 30 C Stop Time: 20.0 minutes Flow rate: 300 wL/min Injection volume: 5 pL Mobile phase componens: Solvent A: 2.0 mM ammonium acetate in ASTM Type I water Solvent B: HPLC Grade Methanol Solvent Gradient: Imow ew m%a8w uBoe xew wmo0 wmww Page Sof19 eee. ENVIRO"MPERNOTUAELCTLNAOBOERSAToOuRYs SMoafstswaSrep:ecAtnraomleytseTMtr:VeMrDsiXonS1c.i4ex" API 4000 Q-Trap Mass Spectrometer Detector Tonization Mode: Electrospray Negative Temperature: 450C GSl:35 G82: 45 Entrance Potential: -10 `Analysis Type: Multiple Reaction Monitoring (MRM) Table4. Acquisition Parameters [comms [am [| oovnum| [NFPAGAGe) | ae | ao | [PEAGAc)| wee 1 meres Tw | [OHA Ace) | ams |weriesnesTw | [[FFOACsAd) | wwe | wmeawows 4| [GAsd 1wo | werwmozes | as | |Cofdd 1 smo | emszmsm | ws | [CucaTwo | wrens | 4s | [Caos--Teme TT smresser | wm | [rasa Tae 17 me Te | [FosA Tas 177 7s wm | [pres 1 ams 1 mame [es | [Peis 1 amo | mawo Tw | [ pros | wee | women ow | [meres 1 ese1 wsaws mw | [[mFreoarl2sGg | | somwse| [a wsmiwwe oo || [Frosiog [sme| wees | ow | `When multiple transitions were monitored,thetotalioncurrent.(TIC)was used to quantify cach analyte, thercby providing greater sensitivity than individual Q3 ion measurements. 4 Analysis 4.1 Calibration 411 CalibrationCurve `Anextractedcalibration curvewasformedbyspikingrabbitseruma prescribed levelsandextractingthecurveinthesamemannerasthesamples. Stock . solutions were individually prepared from neat flvorochemicals and diluted in methanol. Sampleswerequantifiedversus a 15-pointextractedcalibrationcurve. Page 6019 WMEWVIRONMPERNOTUAECLLTAMOB.ORGAST0OuRsYr `Theregression analysiswasperformedusing aquadraticcurvefitweighted 1x. Calibration taidardswerewithin + 25% (230% fortheLowerLimit of caQluiabnrsattaitoinosnta(ndarLdswhwLiicthhOwaecoreQ efnfioct)iweintthoifnde+t3e0r%miwneartieonno>t0i.n9c9l0u.deLdoiwn-tlheev:el calibration curve. Calibrationstandardsthatwerenotgreaterthan2xthemethod blankswere notincludedinthecalibrationcurve,exceptwher noted. 412 ContinuingCalibration Verification(CCV) AContinuingCalibrationVerification (CCV)wasrun a leastonceevery 10 samples,bracketingth sampleswiththecalibration curve.TheCCVrecovery waswithin 225%foralanalytes 413 SystemSuitability Fivesystemsuitabilitystandardswereanalyzedbeforethe initiacalibrationcurve. andafterthelastCCV.Thesesampleshad area counts witha %RSDof<5%and aretentiontime %4RSDof 2%whenevaluatedindependentlyexceptforthe: followinginstances: `Thesystemsuitabilitysamples oreachoftheperfluorinatedacidsandFOSA failed.Ineachcasethe%4RSDoftheretention timeswaswellwithintolerance limits,buttheareacountsvariedbyas mucha520.3%.Thefailureofevery pertluorinatedacidtested suggeststhepresenceof asystematiceffectonthatclass `ofanalytes. Thisshouldbekeptinmind whenevaluatingthedatapresentedin thisreport. 414 LowerLimitofQuantit(aLtLiOoQn) `TheLLOQforeachanalytewasequal t thenominalconcentration ofthelowest standardinthecalibrationcurvethatmetaccuracyrequireanentsof70% -130% andthathada leasttwiceasmanyareacourtsasthemethodblanks. 42 Blanks 421 MethodBlanks MethodblanksconsistedofaliquotsofMilli-Qwaterextractedinthesame m<50a% onafstthhneeLsaLemOpQlresfo.r Athleliofrteshpeemcteitvheaondabllyatneksswiintthhtihsesftouldlyohwaindgarexecaecpotuinotnss: PFHA:Twooutofthree methodblankswasgreaterthan 50%of the LLOQ;nonewereabovetheLLOQ. C7Acid:Oneofthroemethodblankshadareacountsgreaterthan50% of the LLOQ;nonewereabovetheLLOQ. C1 Acid:Oneofthreemethodblankshadareacountsgreaterthan 50% oftheLLOQ;nonewereabovetheLLOQ = FOSA:Two outof threemethodblanksweregreaterthan 50%ofthe 'LLOG;none wereabovetheLLOQ. Page 70019 -------- IW ENVIRONMFENRTALOLNLABOORR0A5CT04OT4R2Y PFBS:Twomethodblankshadareacoc greaterthan50%ofthe LLOQ; oneshowedanomalouscontamination andwasabovetheLLOQ. 422 MatrixBlanks Matrix blanks consistedofaliquots ofblankrabbit serum extractedinthe same `maastnhensameplers. Threematrixblankswerepreparedandanalyzedwiththe samples. Matixblanksconfainingendogenous levelsofanalytethatwereabove the LLOQ are detailed below. Table 5. Endogenous Analyte In Rabbit Serum [sample 1TORA |GiiAch| pres |Tnpros| wHPeDs| [Lcoamgmi)| 0.00504|oouesz | 000506 | 000520 |000503 | [MxBik0011-1| oooes | oows | ooo: | ower | cous | [(MxBik 0505112| oes | ooss | coors | ooo | oom | [MxBic0505113| ooo:| oows | ooorrs | oowe | oom | Pein fr o OEaod TT Gen heCAMA OTEcropen00 . `Theaverageendogenousvalueswereuse 0correctthematrixspikeresponse. 423 SolventBlanks `Methanol blankswereanalyzedthroughouttheanalytical un. Analyteresponse: waslessthan % theLLOQ inal cass. Surrogate PFOA (1,2-C] and PFOS [*0;] were added as surrogates to all serum samples, calibration standards, matrix blanks, and method blanks to evaluate the extraction bmuetthtowdoopfetrhfoermPaFnOcSe.[*A0lyl)PrFeOcoAve[r1i,e2s-w"e'Cr]esouurtrsoigdateer3e0c%ov,ereixetsenwdeirnegwtihtehsiunrro3g0at%e,. recovery range to +/-34%. Specific surrogaterocovery data i presented below. Page 8019 -------------- ee ee-- esee-- esm-- ee-- IM ENVIRONPMERNTOALTLAOBOERAOTONRY Table. Surrogate Recovery Sammpelleei0p ||PRFeOcAoIv1e.r2y-,%%C]|| RPeFcOovSer[y,0% PRFeOcoAv[e1r,y2,-%C]|| RPeFcOovSer[y0,g% [roonomosria| wow [ww TT vwassse | owe | aw | [oomossriz| vox | ow || meas | ew | mw | [uemwososvit| oom |ww | vwasess | ew | wen | [Moowososna| oom | ves | | veasswr | am | ms | [Csosestiet| wanToon TT earom | vee | mw | [somsuse | wow | ow 1[ mwas| om | om | [Foossvies | aon |ox || menses | am | rox | [rocsstist| wn | ow | | meamw | vex | ow | wsosssz | vex | ew [|meee | ew | ex | Wsoeosiss | wow | ew | | eases | eex | ew | [vnssoTewTem TT meses | vex | ew | [ress [osm Tem TTTveases ww| ew | [ewes Trew Ten TT aes ex|ee | [mass ieaw [rm | vwasses ww | ew | [rewsessTew | ew ||Twasess ww | vx | [owaeou [ow[vex |[easm ex |ow | [wages TemTew |] meas me | vw | [meaooso | vow | ee | | Temsm wx | am | [owaseor|"ow | esw |[Temes ww | ves | [owwsors Tew | ven ||Twasem tow | em | [mowers |Tew | ew |T veasss wx|ow | [owassr | TemTrew ease we | ew | [owesTe[ex | ] memes wx |ww | 4.4 Laboratory Control Spikes (LCS) Six Laboratory Control Spikes (LCS)wereprepared in rabbit serum and analyzed with the samples. Results were correctedforendogenous levelsofanalyte in the `matrix (where appropriate), and are presented below. Page9or19 U4ENVIRONM`EPNTRALOLVAEBOCR0AT8T0NO6R4OY2 Table 7. Matrix Spike Recoveries Nominal | Observed [sosesust| ose | os | vem | [is[o oes|oos m u[| s vaa m | [isosostiss| ooo | ose | tom | | Hsosostiss| 308 | ase | ew | [Hsosostisa| a0 | au | wus | [ws | o 30ss | o asss|sviea w | ---- Lsosts1152[|os][om|[I Beossuiss| soo | ae |ww | beosstsz| sos | ar | eee| to% boowsss| so | soe [ome | 1 11 1 11 Page 100119 aw EmRMERTEACLTLANBOOERsATsOaRYs Table 7. Matrix Spike Recoveries (continued) [wCiososmsis | ooamn | |oomme || amomn || [usmosnssTor| om | won | [hT somsiesTaw T 27 1 om | FE EEEEE E EWT YS CS [Coessmoomssiussr[Taooma2 n 7 on | CCorrssoomsurszTi| sasomsT]so toe | cone [(us ss soomso oeaT[Toom oosnnT| e |omoos mm w = w em | ws triosTan TamTom] [Crossooomosssesa|| aaun | | 3aime | owoown|] TCiosomsseiiiseT[oowsTToomm T| waeus|] ov [[iussoossossrtiissa[|20o0m1|2o0a1n || orrusns || [eC somiis ssT[aao moTm sase s 1| var os wne || ux Bc: Cr 1 1 1 on J Fuge 11019 Table 7. Matrix Ske Recoveries (continued) SMENVIROWMR ENTALR LIWBOOERoC AThOeRsY nam. nom [Cmiseemosmiirss[| oammo | 1 oomw [ |wam| ] 2% CCiossewoossnissta|| o2m0 || oaan | swoenx || Cesmosiss| ams sus | som] ----r[Cesosvss-- T2 002T[r| 3 ox | [CSiesoemmmsse|TooomnT |oo eTaa own || 20% Cosommiss| ows Tan os | [wsososuist| a1s | are [oo | --Crsowsa |--aw--1 ----20--|--em | [ceommeon |T owe | o= w |sx | Cocwomnooe | se | 20 | ere| Page 120019 envmosER NTALR LAscRS ATORY aTabvlei7). Matrix Spike Recoveries (continuedandcorrectfeodr endogenous target jafe=r C[ oteo ,|| oeC rsrasodtsor Som [isososrist|ose|owe |oooee | os | sw | Hemera] [us ososties| ose |oss | oooset | om | sosw | w| [o Esmee e|oo | we ow|e oom| ams |wx| [rome sor |am |owes ze |em] r [rMseor mmisusssa||2zor 0r ||22802r || osoowws z2osr | | oamm|] pros [[u1sSoassesmriasa|| oassos||oosaeie ||oomctsertss ooswse ||rwrxe || vwss oossteorrtiszr|| ssooxi||a2enr ||oocoomsss a2m8 || ormsw|| 20% [rirsmosis 04 a1 se |T omens T an | so] on| Seamreer|owe a ome----we --| -- wn] eros Lisomeriss | oars ose| ome om | tom| Msossusi| 312 zou | ome 20 | em | e [Smosrss|osu ome|e oom awe we| Mma | : ETT ------ Ie [reomosnss sez zee| sows 2m 1| om 1| | cNRoeoonerR.ecpoortedSeamsemeaso urablearps5o9f rSytosyesweeves sxAcaevedx. Page 13019 e ee ee ENVIRONMEPNTRALOLMAR0BOR0CA8T4OTR2Y 4.5 Accuracy `The accuracy for each analyte reported was calculatedbytaking the averoafgthee matrix spike recoveriesforthat analyte. The resul's are presented in Table 8. Table 8. Method Accuracy for Individual Analytes [re Tus|csocame m[osacoem]e | [ween 1sz |1 Fesa | wow | [prea |ew |T FOSA | em | [momaTex [pr |e ews| [proATaare|[PFs | Teen | [conc T sox | | Pros | zm | [CioAsd| ww |[THPFOS [| sien | [CiiAsa| sax | THPFOS| +s% | [cease|wun TT 77 1 4.6 Sample Related Comments The extraction and analysisofthe samples was performed uilizinga non-validated `method, The matrix spike results, PFOA [1,2-C] and PFOS ['*0,] surrogate recoveries, and the calibration curve should be considered when evaluating the accuracy and precisionofthe sample results. 5 Individual Sample Data `Tables 9:and 10 summarize individual sample data. Table 8. Prfiuorinated Acid Results |[ar|0e:12aosusers asus aoaralot |aazz |saoises|=oso | 0.0256 [Taser|vs6[oows|ome|ass|orse|oxo |ows | acer| |muaososToes [oooews |oozsa |s72| wi |oss |ores|ocese | [aoa |v0 [ooosos| ooves|sas| vir|oss |ose | oom | [wos rasT<ioalozo | ser| 118| oss |oe| ows| [aoso[ores [oooees|ores |252 | 106 |ore | ors | osm | [rmaooe0 "oss [nosso|ozo|20n|ostr| 0x0 | erat | como| [naove7[oso|<ioolozo|as|ost|ome | ore| cows| [nmeLom Tom [osm |sso Josu |om |oe|oom | TToAe GooSm| oosfse2i|o3oor(resd|5o8 Beol|4WS02 R|oooop6|eos1e5|% ome|ooo | Page 14of19 ---------------------------------------- ` [--es--sa--y Tabla. PoriuornatAced Results (continued) [F|icoorntyT|s0a10r2 ooTeuuetsoesaTlosvaioumsesT]eoaesT|oamsteesn{||saoass s |ooocrmoemwse2{||ocsvioeirse||| [oom ym|ction ow Ts3eTotesTose aia --oome| [[o[kToowrmo | rt32oas | cccuttiiioooann||"oaovmmrea[|aaassose0[[|rataomrr || oazst|eomo [ssw |a || osoors]] soo] [[e[wTaaormmmoT[|arrosns|[TceotuviuoosanTossaeiensse || goo3srssT[|sataaommo||saosmsere||]oaoszssss|| sssoooumwos--]|| |Tunis |~rc2 |ctionTomes |sr |ter |aT ose | sour] [e[iwosises [[|i+ors T|ccctttiiiooooanT|aotvsieossn{|saoseT||aosamemme[|sseserarwr ||a ooavriss ||sssomouoms]]] ([ciaiormsse|||igaoseeosv +t<<iiisaooonTa<ouiia oo|| aaoor[|o-oosmmes||assaaaim t|"oiowosa|| sssooaunese]]] [Fines ~via <iioo|ors| rs om | saw oi sous] [horsw|1esF Tsiioa "oe s a[T omeme] [[[coeosmnovaacrtso occuttiisoonn||oooaguueess[[|ztoiorms[|oooiwseso||oeaozam |ooeiwosse[|[so<imioseona]]| [[Mta edoiaen 11a0asrrTooomoemsT|ooomsueus[|o3s21se |oartes| |s onzosre| o [ooosnm TToeonaowesr| ] [T CSSTaenntes?i| oar us{osommaT]ee Taarms||r1r2 | oss our |om| ous| ERroSAr Page 150019 -_ WEnvIROWMPENTARLLuWSEoOERoAGThOeRsY Table 8. Sample Results for AddHtonal Peruorinatad and Highly Fluorinated Analytes [[T10aas(goimt) 0<.w00o5o01|[0a.0s04ssa|]0o.o05o06|| s0.o15r8||07t1003||u0w0oezno|oo.m0seus|| [[aTemses <<uuooaa||aocaesrsT|aocoomssu[|o16m2 [|a2r4[|<cuuocaa|oones|| [[owweess |<cuuicoaa[|oossmu ||oowoerrz||1110s5 ||au0s||<<uuwooaa[|doooorr|| [tow |<uioa|sors oomes|ass | 16s |cuca |aoe| [iwes0 |<uo0[aie |oowr| az |wo[won|omes| [[wresnss |ccuuoaa[|oowts[[oooommms|ssosr || t1e600[|ccuucoao[oawoss|] [ [aesr m <<iuoooaT|oovme |ocoucoa| ivevm ||2t0as0||ccuuccaa || seocuws|| [(CTomeesz ccuuceo |oewvee[oomom[1smes ||awns cuusoaa|oamomno|| TAs <uoa |oa |nous |am | 1s cuco|om | [r[mT swwAoo|T oomooeo sTToomoo uosw[|0 s3e4r5c||wu 2z5[|i u<ucooooooa am|| [[TTAeGsst [TeTshsA TAs ccuuccaa[|aocweses[|oooomm |2tee0s |z6i6ss|Tc<iuicoaa[|oooumn|| <cuuwiooanTwcoosus [Tooooosoemse||2s0m4 || 241901|<cwuooao||oouursn|| <tioa oom aos|om |am |cuca our| [mss [[wTaeons cuoa |aww T<<iuioco0||oosres |oe Ioocmosm [ ase |m1|uo 3s1m0 || twem3a|<<uuccoo ||oooomr|| oo] [[emesras <<iiioo0a|[oostu oommeerr 2 oso|| 2w2u1sT|u<cuooo||oooouos|| [ [keem i Tuccuioo0[|aoowe (owooro[|1i5se3 || evezz ||c<iuiccoo||oomseer|] rey ee on Eo A ma TT Page 160119 BC , Enum oro 4 1% PROJECTNO.E0S-0442 Table8. SampleResultsforAddonsPririated and Highly uorinatsd Analytes (continued) [Coamno0moi [Zooenutna[oaoise]coaostes]oramr s|rartes)[cnopos oauss|| [Tae |<uoco [oor|<uwoo| 1a | ar |<uoa|oss | [Mean Twa [ows [ootees | 258 | as| na | cosa| [[MoeI dwan To|"e"7 nsaT[0r1o7 2c4a |sY ou0eisor[| a28es ||2issT s[|NnTnaomCooTses|| A[T osT sor viE nl1et T [ooycT arveg|arseor)|4a.s|on|omer| Table 10. isomerRatios for indiiSdamupslets BFrcaoRnmcehOred||| BF"rlRaenOocmSheTerd mweswT e]w ce ae we oo |n eam]] eweer | Teme ai eaan mm|e a ex]] mmeeow eveees Taanm Taaw]] meeess veomm TTaam m[ooew]] [CC wessr ews | aioe | wom | wtosr | asmese|aiomn{[oanm || I wes-- T ew 1Y wor | ex | T mnoemss c ces T Taae mmT|aae]] IESIT Ioo-- n --ew---- ow -- | I ST Page 170119 e ------e ee ------ ENVIRONMPENRTALOLAhLBoOECRoACsTtOTeRrY [wes 7 evn Tien [sew | [wee[vo | aoa | s ww | [amet | vena | aioe | sm | [owes[ve | awa |s am | [wero eva T aioor | aw | [wer wed | aw | aes | [twee 1 Wassacheew | ior| ew | [we | eschew | ose |a | [oes Tem | osx | am | [owes "ems [aio | am | [owes Gem | wow | am | [owes[ve wow | s ww | nr --T [T Aww T awe | am | "The branched omer he simple|wis<cLLuOQrsor | wT | aw | "++V+TTNh.eA-aGve5r7a7gaeanndd6s9t7an8daardfdervaimattihoensrapmteesloctotfapnoyolheodsseesraunmp.leswith oervaluesabove theLLOQ. 6 Data/Sample Retention AElavoirriognimneanltraalwdLaabtoaraatnodrtyh.eaTnhaelytteistcamlarteeproiratlshaanvdebaenaelnytairccahlirveefdeartentchees3taMndard reserve samples, as well asthesamples pertotthiaspriojecntariearnchigved at the. 3M Environmental Laboratory. Rreetsaeirnveed sinamtphleeasr,cdhiigvietsalocfo3piMesEonfvoirriognimneanltdaaltaLaabnodraatloroyrfiogirnalpepraipeord doatafa wlielalstbe: 10 years following report signing. 7 Conclusion `prUensdeenrttihne calolnsdiatmipolnesso.ftNhFePAst,udPyF,OeAn,doCgsenAociuds,fCliuoorAoccihde,miCcyaylAscwiedr,eFfOoSuAnd,tPoHbSe, PdeFtOecSteadndinTaHlPbFutDStwhreereesadmeptieecst,edanidn tehveerCyzloAtocfisdewrausm.detTecDtHedAinan3d0PofFB36Sswaemrpeles. Cdeotnevcetresdeliyn,1PofF3H6Aswaamsploensl.y dTeHtePcFteOdSinwa9osfno3t6dseatmepclteeds,inanadnyFofBtShAewsaasmpolnelsyusing the present methodology. PFOA [1,2-C] surrogate recoveries were all within 30% `fwohriilendPivFiOduSal['a*n0a,l]ytseusrrroagnagteedrefcroovmer+i/e-s1w%erfeo-a+l/l-w2it7h%inwh+/i-le34o%ve.raMlletmehtohdoadccuracy uncertainty (based on isotopically labeled surrogate recovery) was +/- 34%. Page 180019 awENVIROMTENRTAACLTLANBOoRAoTORrY ETE TN FS Trees Toi BhRR 2d T_end s2p fo1/ e 05 = WaleReagan, LaboratoryManager /2/63/5 Tots "The 3MEnvironmental Laboratory's Quality Assurance Unithasaudited the dataand refoprthoisprrojetct. Qualty. Representative 12-1- 2068 Dato Page 190019