Document RaZQmVzyq6Ee6mx0vo5zGLvpV

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorcoctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component ofFC-600. (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 628. The testsample is FC-600 (US) (cc8212-19).Currentinformatioinndicateistisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultonefoamer, 1.00% sodium decyl sulfate0,.85% xanthangum, 0.1% N-(3-chloroallyhle)xaminium chloride, 0.80% starch,and 0.05 % benzotriazole. The followingsummafy applies toa mixture with incompletely characterizedconcentration of impuilties.Data may not accurately reflectthe toxicityof the fluorochemicalcomponent of the test sample. METHOD: Method: Not noted. Test type:Acute stati(cRange-finding) GLP: No Year Completed: 1983 Species:Pimephalespromelas Supplier:Commercial supplieirnBrady,Nebraska Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: Not applicableN.o mortalitaythighest concentrationtested. Test fishage: Juvenile Average Length and weight: Length = 3.6cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment:Not noted. Test conditions: Dilutionwater: Carbon-filterewdellwater,St.Paul,MN. Dilutionwater chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 180C Lighting:Not noted Stockand testsolutiopnreparationT:estsolutiownesrecreatebdy directweightsadditions. Concentrations dosing rate:Once Stabilitoyf the testchemical solution:Not noted Exposure vessels:Wide-mouth one gallonglassjarscontaining3 liters of testsolution. Number of replicates:One Number of fishper replicate:5 Number of concentrations: Four plustwo blankcontrols Water chemistry during the study: pH range (0- 96 hours): 8.0- 8.2 (controlexposures) 7.7- 8.2 (1,000mg/L exposure) Dissolved Oxygen range (0- 96 hours): 5.8- 9.6mg/L (controelxposures) 0.9- 9.6mg/L (1,000mg/L exposure) Temperature range (0- 96 hours): 18 - 21OC RESULTS Nominal concentrations: 2 blankcontrols,1,10,100, and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L Element value based on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The value reportedappliestothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hour LC50 forPimephales promelas was determined tobe >1,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. The studymeets allthe criterifaorquality testing.However, the sample puritywas not properlycharacterizedand the studylacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 8875, 1983. OTHER Last changed: 6/28/00 ENVIRONMENTAL LABORATORY AOUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST) LR Type Testako@,, -A,,, Test Organism,@@@ aterial Testo 012rce IVIN t-.*'Ol@)@Ill Lot 5/ 4- -rype- (4fe PW-@- /17 ate Recld-.aZ5 atc-@No.L-6@s Avg. Wt. I-.7A-V grams Avg. Sizq;5&cm No. Organisms per dose 5 Test Solution Volume 3 Liters/All-Glass Tank Tank Dimnsions:W IDE-MOUTH I GALLON GLASS -JAR Time Started- f Date Started- g J(p Zu - Date Complete .z ///I Dilution Water Carbon-filtered.wellwater (Temp. 0oc. D.00 /,!g ppm. 'PH-a2 Investigator (Full Signature) Lcso (95% Confiie2ftL@Leimits) 1!0 Tnit. ,k zone. Control Total Amount Added 24 Total No. Deadl urs Do H opom 48 Total No.Dood urs 7Z ID-0.1 Total H ppa No. De LH 96 0 tal 0. @-,O-D'e.ad H t;m qI Ao Test Temp. Inutimig Date Co@nts: Effects other than Mortality Tank No - Symptoms__or Reactions Control TT 24-Hr. EC so Total No. Affected 48 Hr. 72-Hr. WHr.