Document RaRzON4n7RbqvXdLvvmGmQZB8
A # 22 - (S 7 /
Analytical Report
\ Occupational and Environmental H ealth
Analysis o f ^frfiuorooctanolo Add (PFOA) In Human Serum Samples
\ Exygen Report No. L0003000
\ Testing Laboratory
\ Exygen Research \ 3058 Research Drive Stale College, PA 16801
\
\ Requestor Dr^tamha BaCey Occupational and Environmental Health 4 RcsiemarCircle Parkarabui^ WV 28101 (304J-757-0270
\ \ \ \1
\
\\ \
m an cm
E ID 8 7 1401
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S .'fc S g R 'fc6801, Tf:t8801042387123.1201199
USA
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Reauta >reportedlor9 analple atpeDuorooctanoteadd(PFOA) h humanaarumsamples received 1 fi^gen from Or. M int Bafleytt Occupational and Bftonmantal Heaih. The Btygenprojectnumb*aaalgnedtotheampleis P859.
A total at Iwatw aamp* were received at Exygen In S mL acmwcap vlaia labetad wtti pamunantmarker. A copyat a l aamptatag-lnInformation la presentedIn AttaohmantA Tha hrotoi samptaswere recaiwd on07/23/0*. Thesampfca were shipped frozen on dryice viaFedEx. 71eampleawerestoredtroaritorntimeotrec|p*ijnt9analVi.
3.1 Sam ple Preparation
T2par1pho1per.JapraFatmotltutlpyoslnemacfowclruuapmrtieltneoar.tettraoacfcastteaomdnfpctildeewmeaaesswsaededddeadfcoctroetnhtttiewnegaxtatormalchptatiaodntaanprderontchtearednvufprceat.aanaUaadstimntogemtthhuoegdtMiEaUxpMSrtpoortooehiane-
3 4 Sampta Analysts by LC/MS and LC/MS/U3
In High PleasureUqpH Chromatography(HPLC),analiquotofattractfc tajecttdandpaaaad
though a tiqukH*wae clmnittograptife ookm Baaad on foa aRMyof foe analyte fortf afeft^phM JnM coktrnrriatiM totoiqUdrnobtiaphaa, to anafctelaretainedfora character!*} amount of tim*. Fntiowtog HPLCaapanttan, maaa apadramatry provMaa a
repErWHeurele maana lor analyzinga wide mngaof orgenb compound*. Molaoufaaare
lontact fragmented and detected. Tto lonecfuradarittic to corioutti are observed eittguarttiatodagain attractedetandarda.
AnHP1100 ayataroInterfacedto a PE SctexAPI4000systemwas isedtoanafyza the sample extra*. A gmdant elution through a <fonaa Chromatography Gamete C-8 SOx 2.1 Rmxapinoolumnwaauaedforaaparetiori.
Ttofotiow foggredfonttw a perform ed
MoMe Phase (A}: MobleRiaae{B):
2mMAmmoniumAcetatein Tjpe I Water Malhanol
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am a
NegaSve 418*308 (PFOA),415*370 (" C-PFOA) 350*C 7.0 bWn
4.1 C illb ratto n
A & p o tt eribntion cwve w wwftfzad thnughout ha anaVticai aaquenca tor ihe auorooonyoundfc Th* cafenon poMa wam preparad atlas, 1,8 , 10, 20, 50 n g ^ for PR3A. ThalnatnjmartwdlpofaaivanwjathaooncrtnUk^WMplottsdforwK^pQfe. Uaing tc m r ragnMialan *h 1A waigMiao, (w ataja, y+rtorcapt and ooeOoianto detamhaon (o viwradataimkMCt AcatoifenaM <Baooaf*al*Vr*fca885.
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4.3 Laboretory Control SpDcea
Laboratoiycontrolaptas h i amtyfcalaatw m pnparad ty addng a towwnoonembadon cttha analtas toocrtrolhumanaatun. UbondoiycantrolapBaaMuaadtoaaaaasmaNxl
aew ao^ ^ laborttovoortret aptaa nuat aiiow raoovariaa batwwan e a ra m fcr tonta at 9 10Q and6S-115%forlavatagreatarhan ha LOQ och a data it rafacted For tha ranea report hsretthaapfcaaara**Hn h a accaptablaranga.
4.4 RtaMxSpIlOM
TV mattc apta* h # analydeafaatwarapnparad ly addhg a InowncomanMion ottha taagManalytetoaapantalwnanaamaiaampiaa. Matifecaplcaaaraussdtoaaaaaamahod
accuracylnhamsttiL l1jiMftfcapl(ssatatt4roreaovwtesbelMtBen65-119%. Forlha reaur*wtadhara,hema(p*wswarew4hlnhaacaiptiitilefaf^. "
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4.5 Sam ple R elated Comments Three samples in the analytical sat were extracted and analyzed in dupfcate. Dupfcate samplereads amporteltongw#hthesampleresultsh Attachmenta.
Pleeseaee Attachment Star a detallad toting of the analytical results. Results are reported In parts per bfflton (ngftnU toranalyse on an as-recehredbasts.
Samples are dspoead ofonemonthaftertha reporttoissued unfaasotherwisespecMsd. Al stsctrenlo data to archfvod on reMavebls media and hard copy reports are stored in data Aidant maintained by Erigen. Hardoopy data to stored or a minimum of (toe years. Occupational and Environmental Health wfl bo nettled 30 days prior to the dbposal of hardoopydata. f' 7.1 AttscTiriantA:ChainofCustody 72 Attachmenta AnalyticalBeaut
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MONOM
EID871404
7
3058 Research Drive State College, PA 16801
Phone: 814-272-1039 Fax: 814-231-1580
L o q in
Login Group; 10003000
LPorogjinec#t:
3110 P00009S9
Company Name: DuPont da Nemoure & Co, tow.
Submitted By. Chartee R. Pawley
Login Type:
Immadiste Receipt of Samples
Started:
T
Date Stare
07/23/2004
Due Date;
08/00/2004
Received Data: 07/23/2004
Received By: Ammerman, Marie
Spread Semple:
Conform COC Sample: True
Conform COC:
True
Conform Sample:
True
Conform Request
True
Oxygen SD/PI: Decker, Emfly Project TWa/Type: Analysis forPFQA in Human Saturn Samples by LC/MS/MS / ROUTINE Login Notea:
Conform Note:
P H h w Cu b a PKOOOTB
Package / Containers
M P f rCondition
SI*** ID
7/23/04 338:30PM Padae* *C m (** UneempremlMd
FEDEX 343330934333
Xw itt SagtrettlEinte
Diy lea 0.0
RECEIVED Anvnerman, Muk
Contain# C0040BU caoMaas eoo4oaaa coownt f'ffVftffM C0O4OSM MnceMM. C004M4t
CQCHQIM
CtfflUHHI
Stow Weight 4.00g ' *1
4.70e 4 . g
4J0e 4.30s
440.8 4.S0S uot
4jo s uot 4.00g
B tt
ContainTvne 5n* arum tube In * arum tube In * mum tube Jmluramtub* 3ml arum lube 3n* nun tube
..ItflJSWRSfo 3mlarum tub*
Jn* anm tube 3ml arum tube In * anm tub* Striatum tub*
Freatvettw
NONE NONE NONE NONE . NONE NOME NONE NONE NONE
NONE NONE
NONE
Mfe- Lat
Mfa. >0
7L/o2g8i/n0-4ipt
Report Version: Jui 1420041 t :39AM Page 1o f2
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Irmence:'.
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SwetlM Sa/nnla Human Sanan : '
DataSnmmadDamHauiiMd gaiOu 071221200a 07123000a 04/06/2004
UQUO Human Suum LIQUID HumanStoini .
C O N FID E N TIA L IN FO R M A TIO N
07/22/2004 0103000* 07122/2004 07/23/2004
06/06/2004 oa/os/2004
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LIQUID HumanSanan UQUN3 Human Sanan uomo HumanSanara . UQUta HumanSanan uom o HumanSanan LIQUID HumanSanan uomo HumanSown
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07/22/2004 07/23/2004 08/04/2004 07/22/2004 0703000* 06/040004
07021200* 0700000* 04/06/2004
07/22/2004 0703000* 04/04/2004
07/220004 07/23/2004 04/04/2004
07/22/2004 ' 0703000* 04/04/2004
0700000* 07/23/2004 04/04/2004
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COLLECTION SUE INFORMATION
Collection SR* (ram * & addranR `IX_J__T _h_r_s_h__a lb a .i)c y --------
. g - H W r * m r C i n r f r --------U ir r r r s b J r c ] yu i V
Wiono: ^ O U ' 'T `b l - C n n -------
j STAFF INVOLVED IN SAMPLE SHIPMENT
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TSDEUSS-
IMPORIANE Each person who enterslnformattoo on Ms form must sddtheirname,signature, Initials,and date
ebove. Une out unused prompts. SpecifyAM or PM fortimeentries. Indudemonlh,<fa anayearfordeles. Contents of
p ad c^ r r ^ t)e vewrHifiehddbqy someone other ttlun theppaacckkager; attest to the verification by htoititiaafflinfand dasttilnng* the
p rw n p tO TTrou|h(v documentthe reason forany me gapbetween the me the serum preparation Iscompleted
snd the time packaged by adding a footnote to the bottom of the page.
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CONFIDENTIAL INFORMATION REDACTED
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V H B 3H L
E w g_ eRESrEArRCH
Aredai Resswdi. \ F W n ReaitaL
Summary IonfHRurnmriadnunSFwouumndS(anmgAplnnL)lor PFOA
SpoPnsor
iPwFO*A)
CONFIDENTIAL INFORMATION REDACTED
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\ \ SpoIDM or INFORMATION
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PFOA 4111000M934000--
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