Document RaRQ4e6m4kgxjodJpqq33Zw9E
AR226-2785 """""i^sss
FOR DU FONT USE ONLY
Copies to:
Haskell
E. I. d" Pore de Nemours and Co., Inc.
Laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50,
Newark, Delaware 19711
Medicine
HASKELL LABORATORY REPORT NO. 788-82
Material Tested* Uctanoic acid, pentadecafluoro-, amfflonium salt
Haskell No. 12,037
Study Initiated/Completed 2/3/82-1U/15/82
^ Material Submitted by
CR&D Department ' Haskell Laboratory
ACUTE OttAL TOXIC ITY Of[
A FUNCTION OF AGE
ms Summary: Ammonium perfluorooctanoate
single oral doses to the
was
slightly
toxic
when
administered
in
Bales,
LD50
--
573 mg/k^
following groups and females, LD50
of rats:
580
weanlings (21 days old):
a-lLf weeks rat-s:
)ld): males,
LD50
LD50 =
470
mg/kg
=
and
nig/kg; young females, LD50 =
adults (approx. 453 ing/kg; older
males,
days old): males,
in regards Lo the
= 336 mg/kg and females, LD50 LD5'J = 243 mg kg and females,
sex ot th^
= 343 mg/kg ; newborn 2
LD50 = 258 mg/kg. Toxicity
ynoeuwnbgornad(u.<lt 2 radatsys. old) and older rasthsowhedad nolowseigr nLiDfi5c0anvat ludeisffetrheanncew.eanHlionwgesveorr,
f 'cedure; The
b,.' intragastric
test ."late-rial, intubation in
as
suspensions
in
corn
oil,
was
administered
groups were (1) Male -
single
Newborn 2
doses
to
groups
of
Ctl'.CVl
rats.
The
old); (3) Male - Weanlings
days old); (2) Female (21 days old); (4) Female
Newborn
2 days
old); rats;
^5) and
Female - Young
(7) Female
adults
(approx.
8-10
weeks
- Weanlings (21 days
old); (6) Male - Older
- Older rats. A Range Finding Study was conducted to
draettesr*m*.ine Ththe e suinrvitiviainlg droasets lewveerel wfoerighCeude aLnDd50obtessertveodn dNuewribnogrn a 14-2dadyays old)
trheecomveoryrtapleitryioddataand usthinegn tshaecrmifeitcheodd . of ThDe. JLD. 5F0 invanleuye.s***were calculated from
1 -
TSCACBl Sanrt.zed.0oes"ot contain
Coinp1^
Results;
Dose
(mg/kg)
Average Weight
Male - Newborn
Body
(g)_
2 days
Suspension
W__
old)
Average Dose (nL)
370
6.2
330
6.1
280
6.4
240
6.2
200
6.9
13u
7.6
1.0
0.23
1.0
0.20
1.0
0.18
1.0
0.15
1.0
0.14
1.0
0.10
Deaths occurred up to 6 days after dosing.
Mortality Ratio
10/10 10/10 10/10 4/10 0/10 0/10
LD50 ****
2*3 ag/kg
Female Newborn 2 days old)
320
5.8
1.0
2HU
5.7
24U
1.0
220 b7..12 11..00
210600 8.0 1.0
130 76..70 11..00
Deaths occurred up to 4 days after
0.19 0.16 0.15 0.15 0.16 0.10 0.10
dosing.
10/10 6/10
5/10 0/10 0/10 0/10 0/10
258 ing/kg
Male - Weanlings (21 days old)
710
6a
67o
82
525
69
450
87
-*00
84
350
87
3.0
1.62
2.5
2.21
3.0
1.20
2.5
1.56
2.5
1.34
2.5
1.21
CDelianthics aol ccSuigrrnesd: upSta';ione3d daaynsd awfetterpedroinseinagl . area,
8/10 7/10 3/;.0 2/10 2/10 0/10
weakness and
573 nig/kg
weight loss.
Female Weanlings (21 days old)
b7u
80
450
87
2.5
400
80
2.5
350
82
2.5
2.5
Clinical Signs: Stained and wet
weight loss. Deaths occurred up
2.14 1.55 1.28 1.15
6/10 3/10 3/10 1/10
perineal to 3 days
area, after
stained dosing.
face,
580 ing/kg weakness and
^TSCACSi SanUns'J, 0^^r"'-.'lcon"-'"
Comply
Male - Young Adults (approx. 8-10 weeks
470 og/kg
Female - Young Adults (approx. 8-10 weeks old)
670
185
500
185
425
182
350
180
5.0
2.48
10/10
5.0
1.85
8/10
5.0
1.54
2/10
5.0
1.26
1/10
Clinical Signs: Stained
discharge, diarrhea and
face,
stained
and
wet
perineal
area,
dosing.
weight loss. Deaths occurred up to 4
453 mg/kg
nasal
days after
Male - Older Rats
72U 500 4UU 350 3UO 24LI 20U
Clinical tremors,
occurred
563
10
4.06
558
10
2.79
543
10
2.18
564
10
1.98
501
3
4.95
644985 33 3.96
4.32
Signs: Stained face, stained and wet lethargy, chromodacryorrhea, diarrhea up to 9 days after dosing.
10/10 10/10
10/10 9/10 0/10 0/10 0/10
336 mg/kg
perineal area, weakness, and weight loss. Deaths
female - Older Rats
f)7u 450 40U 350 225
Clinical
tremors, cosing.
335550 1100 21..3640 190//1100 343 mg/kg
365
10
1.46
6/10
358
10
1.26
5/10
346 3 2.55 1/10
Signs: Stained face, lethargy and weight
stained
and
wet
perineal
area,
weakness,
loss. Deaths occurred up to 5 days after
-
3 -
noloo^,nTSCAC3l
Sanit^d-Does
Cow?^
** tonge-flnding Study Results, Newborn Bats;
Dose
"g/te
Mortality
Male
130
0/4
210
0/7
300
2/5
375
4/5
425
3/3
500
1/1
575
7/7
*** Finney,
Press.
D.
J.,
Probit
Analysis,
3rd
Ed.,
1971,
Cambridge University
**** 952 Confidence Limits:
Male - Newborn 2 days old)
female - Newborn 2 days old)
lower (mg/kg) Undefined
244
Male - Weanlings
(21 days old)
514
Female - Weanlings
(21 days old)
470
Male - Young Adults
40J
(approx. 8-10 weeks old)
Female - Young Adulcs
(approx. 8-10 weeks old)
413
Male - Older Rats
Undefined
Fem&ni - Older Rats
285
Upper (mg/kg) 276
55.7 19.9
664
8.0
2,258
4.7
536
8.6
503
15.0
390
8.0
--*-
.
ts"&!?^j^fg - i "iPE'i-;'-}
.-
4 - Ciwipany Sanit^od. Does not conlain
^s^s-^SeSas'isS,
Report by:
an Hind Technician
Reviewed by:
0. Louis Dashlell Study Director
Approved by:
^gL.^-. ^ ^^..^ .
Gerald L. Kennedy1 J^
Section Supervisor'
Acute Investigations
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