Document Ra75OJbQVo6rJL8ar89EwaxBv
n- edtor
AR226-09FF
Industrial 1B8I10OF-RTONETSATGELaRbOoAraDtonies, Ine. NORTHBROOK. ILLINOIS 60062
REPORT TO
3M COMPANY
REVERSE
MUTATION T-1485
STUDIES
WITH
IN OFINVEESSAALCMCOHNAELRLOACMESYSTRSATIRNASIANND
P.O. NO. LP-003397-400
AUGUST 10, 197
o
1BT NO. 8540-09238
z= 320
2G
5iN
Conizin NO 0B)
414
Y
Industrial 1B 810 -I FRTOENO TSATGELzRbOoArDatovies, Dre,
NORTHBROOK. ILLINOIS 60062
August 10, 1976
Dr. James E. Long Manager, Toxicology Services 3M Company 3M Center St. Paul, Minnesota 55101
Dear Dr. Long:
Re: TB-T148N5o.in85F4i0v-e09S2a3l8mo-neRlelvaerStsreaiMnustaatnidonOSnteudSiaecschwiatrhomyces
Strain = P.O. No. LP-003397-400
We are submitting herewith our laboratory report prepared in
connection with the above study.
Very truly yours,
Jccibp
J.C. Calandra President
2 9/5
| Industrial BIC TEST Laboratories, Jue
REPORT TO
3M COMPANY
REVERSE MUTATT1I4O8N5 STUDIES WITH IN OFINVEESSAACLCMHOANREOLMLYAGESSTRSATIRNASIANND
P.0. NO. LP-003397-400 AUGUST 10, 197
IBT NO. 8540-09238
IL mirodsetion
A sample identified as T-1485 was received from 3M Company for
the purpose of conducting reverse mutation studies. The test material
was evaluated for genetic activity in microbial assays with and without the addition of mammalian metabolic activation preparations. This report presents the results of the investigation.
66
Industrial BIO-TEST Luowbries, Jac.
2
1. Summary
in sTehreietseostfmiatveirtiraol,miTc-r1o4b8i5a,l waasseayesxaumsiinnegdSflormomunteaglenaincd aScatciveihtayzomyces
indicator oxganiarss. The test material was tested dixecty and inthe pre.
cence of liver microsomal snayme prepssations om Aroclor-induced rate,
bDootshethleevnelosmaocfei0v.a2t5i,on0.a5n,d
5,
the
aancdti5v0atuigonofesTt-s1.485Sopmere
plate were used in
physielogica effec
Levels were below tose Loves. was observed at the highest dose level (50 ug/plate). The lower dose The results sbsined with the positive conteol materials demonstrate
hat the tet systems are functional with moun matagens.
The results of the tests conducted with T-1485, both in the absence and
in
the
It
presence of the
was concluded
rat
that
liver activation
T-1485 did no
system, were
exhibit genetic
all negative.
activity in any
of
he 12 ire assays employed in this vestigation.
Respect submited,
INDUSTRIAL BI0-TEST LABORATORIES, BC.
Report prepared by: St ein sHeteade Toscot
Report approved by:
=,
2~
~
2 ne
Sinaier: Torkeiony
som
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--
---- The following strains of indicator microorganisms were used
5
2. Bacteria Strains: Salmonella typhimurium, strains
TA-1538 TA-98 TA-100
The tissue homogenate and 9,000 x g cell fraction were prepared
from the livers of adult male Sprague-Dawley rats. The animals were
treated with 500 mg/kg of Aroclor 1254 five days before kill. `a sufficient
sucrose and completely homogenized with a motor -driven homogenizing
from thts preparation were weed dor the activation teste.
498
Jndustrial BIO TEST Loboralories, Inc.
4
G. Reaction Mixture
testa: The following reaction mixture was employed in the activation
Component
I2R 1. vTTePeoNyoe (tsodnim eunma saalt) Sl hCDaeveirvalheonmto8gen2s5tmegtroacktiton D. Plate Test (overtsy Method
Final Concentration/ml
2365H6 ipbpsmmoottmloeeensns
strain weArpepradodxeidmattoelteyst10t9ubceesllcsonftraoimninaglo2g.0phmalseofcumlotlutreenoaf geaachsuipnpdliecmateonrted with biotin snd a trace of histidine, For nenactivation tests, the 4 dose Levels pofoutrheedteosvtermattheerisaulrfwaecrese oafddseedletcotitvhee acgoanrtepnltasteosf.theInaapcptriovpartiiaotnetetsutbsesthaend 5,000 3g tissue supernatant and required cofactors (reaction mixture) were
added
added
to
to
the
the
overlay tubes. Four dose levels of
appropriate tubes, which were then
the test chemical were
mixed and the contents
poured over the surface of a minimal agar (selective medium) plate and
allowed to
snd scored
for the solidify.
The plates
number of co
were
lonies
i
ncubated
growing
for 48 to 72 hours
on each plate, Bos
at 37
itive
C,
and
solvent controls were run with each assay.
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| Jmthsinial SVQ 1x31 Laborsionies, lnc.
5
E. Positive Control Chemicals
Positive controls using both directly active positive chemicals
and those that require metabolic activation were run with each assay. The following chemicals were used for positive controls in the nonactiva-
tion and activation tests:
Test Nonactivation
Chemicalx Methylnitrosoguanidine 2-Nitrofl(uMoNrNenGe) (NF) Quinacrine mustard (QM)
Solvent Water or Saline WDaimteetrhyolrsuSlalfionxeidests
Probable Mutagenic Specificity
BPsx FFosutr
Activation 22--AAcnetthyrlaammiinneof(lAuNoTreHn)e
Dimethylsulfoxidess* Dimethylsulfoxidess#
BPS#k FSuk
o
8-Aminoqu(iAnFol)ine (AMQ) Dimethylsulfoxidews* Dimethylnitrosamine Saline
BFiSsH
(DMNA)
#4* CBoPnSce=ntBraastei-opnasirgisvuebnstiintuRteisounlts Section *#% PrFeSvi=ouFsrlaymesshhoiwfnt to be nonmutagenic
7 420
Lee Site ssi, ne.
6
IV. Results
A summazy of the plate test results is presented in Table 1, The
test material was evaluated at a series of dose levels such that the highest
level (50 pg/plate) showed some physiological effect. The lower dose
levels (0.25, 0.5, and 5 ug/plate) were below toxic levels. The results
obtained with the positive control materials demonstrate that the test
systems are functional with known mutagens.
The results of the tests conducted with T-1485 were all negative.
o 42/
Industrial BIO TEST Laboratories, Inc.
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