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AR226-0500 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; mayalso be referred to as P11F6O, AFCa-m1m26o,niFuC-m16sa9l,t,oArmFmC-o1n4i3.um(pOecrtfalnuooircooacctiad,nopaetnet,aPdeFcOa,fiFuCo-ro, ammonium salt, CAS # 3825-26-1) R14e3m.arIkt'ss :puritTyhweas3Mnoptrsoudfufcitciieonntllyotchnaurmabcteerriwzaesd,8t3h.oTuhgeh test sample is FCcurrent information ainnddicCastepseritflisuoaromiaxntaulroegoufe9c6o.5mp-o1u0nd0s%.test substance and 0 -- 3.5% Cs, Cr, METHOD: Method: Not noted. Type: Static acute GLP: No YSepeacriceosm:pBlleutegeidll: s1u9n7f8ish (Lepomismachrochirus) `Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Not noted Test fish age: Not noted. Length and weight: Average length =3.0 cm Average weight =1.2g Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Temperature: 19C Dissolved Oxygen: 9.9 ppm pH: 76 Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stabilityof the test chemical solutions: Not noted Exposure vessels: Tanks containing 16 liters test solution. Number of replicates: one Number of fish per replicate: 20 Loading rate: 1.5 g/L. Numberof concentrations: five plus a blank control Water chemistry Dissolved during the study: oxygen range (24 - 96 hours): 5.6 -- 6.5 mg/L (control exposure) 49-59mg/L (750 mg/L exposure)" PH range (24 - 96 hours): 03744 +750 7.8 m7g./6L7te.s8t ((c7o5n0trmolgleLxpeoxspuorseu)re)" group (third highest concentration) data given because total mortality occurred in the highest test concentrations within 48 Thoeusrts.temperature (24 - 96 hours): 19-20C RESULTS Nominal Element vcaolnucee:nt9r6a-thioounrs:LCB5k0c=on5tr6o9l,(452000, 560, 750, 1000, ~636) mg/L and 1350 mg/L Element value based on nominal concentrations CONCLUSIONS TmhgelLtewsitthsaam9pl5e%9c6o-nhfoiudrenLcCesoinftoerrvballueogfil5l0s0untfois6h36wamsg/dLe.termined to be 569 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: followed. NKoiiimnifsocrhmartainoknionng 3s.toTceksotrintgesltascoklsutiinofnorpmraetpiaornatoinontsh.e method Sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rM38C4o4m.pany, Environmental Laboratory, OTHER Last changed: 5/24/00 0374S T ST EA ORINRHTFo t RO Gaia Eo Sa ANTE RCLETY LLlozx sas ENVIRONMENTAL ENGINEERING LABORATORY "AQUATIC TOXICITY DATA SHEET 1 ee Cu 32 YR use Ola Howie Ham teria ested F143 100 22 # * Tost orpmisn_RlugidtSunditn Ave. We. 1,2 grams Avg. Size__3,0 ca "1. Test Solution Volume |( Liters/Tank No. Organisms per dose. 20 : Time Started_ 2:00 Py Date Started _S ~2 7Zb3 ats Comploted__S- 22-79 Dilution Nater_Carbon Filter - Well Water (Temp. [9C., 0.0.0.9 ppm, pt 7.5 ) Ee Te lone alee ,. Investigator M.7.fleaSpiny LC 50 (95% ConfidenceListes)at aes. Sf ro 3 = Tu - meal 200 Pfec]l \ Posen] | osfsell | Jrs]cs] nee]|holes=]Jogleo=]lTasca] 4] stoll 2012Delco] 2| [celloPadlcell 16foalss] yee =e]|5 bell]2 elsl 11[alc 14 [ool od15 Tolssl| 20for[So] ao] = [20] D2 NEEWE 7Ed | ET XE 0 EaEE Cr rrr Cr rrr rr a I |NN A e. 1 18%c [I ec J ec [| ae | Comments: EE -------- ee---------------------- e--e --t -- _-- _-- -------------------- eees-- eemsee-- seesse-- -- --r --e -- BESTCOPVAVALABLE --_ -------------------------------------------- 03746