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- AR226-2881 B. X. du Font de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 58-80 | MB--------fe--------i Haskell No. 13197 Study Initiated/ Completed 9/12/79-12/10/79 Chemic Jackson ORAL LD50 TEST IN BATS Procedure: The test material, as an aqueous solution, was administered by intragastric intubation in g-roups of 10 young adult ChR-CD male rats in single doses* A range--finding Study was conducted to d level for the LD50 study+. The surviving animals were weighed and observed during a 14-day recovery sacrificed. The LD50 value was calculated fronr the mortality data using the method of D. J. Pinney. Results; Dose (mg/kg) 14,500 Average Body Weight (q) 254 So]-ution (%) 80 Average Dose {ml} 4.60 Mortality Ratio 6/10 14,000 265 80 4.63 10/10 Clinical Signs Stained and wet perineal area,s lacrimation, salivation, diarrh posture, weakness, lethargy, an weight loss. All deaths occurr 4 days after dosing Stained and wet perineal area, salivation, diarrhea, eyes half lethargy, and severe weight los deaths occurred within 3 days a CTOS^,fee<ft a^no Reeuilt-s: Doaa (mg/kg) 13.000 (Con ' <; ) Average Height 261 Body (g) Solution (%) 80 Average Dose (ml) 4.24 Mortality Ratio 10/10 12,000 252 80 3.68 9/10 10,000 258 80 3.22 2/10 Clinical Signs Stained and wet perineal area, st face, diarrhea, congestion, chrom weakness and severe weight loss. occurred within 7 days after dos Stained and wet perineal area, s tacrlmation, chromodacryorrhea, diarrhea, weakness, lethargy, an -weight loss. All deaths occurre 4 days.after dosing. Stainea and wet perineal area, s ''weakneiBfi^. lethargy, and severe w ~":.' Both deaths occurred within'4 'da adainistered orally to young adult ChR-CD male rats in-" single doftes;'its-UD50 Is 10,6'9B 'iag/kg of bod signs seen at most dose levels included: Stained and wet perineal area, .stained face, lacrimation, s weakness, lethargy and severe, weight loss. All deaths? :pebuacr^d- within'7 /(Says after dosing* Synonym: + Animals were given individual doses rari^irig froitt eTS^Si^O'dtf^^r/ligt'^^OeaCli^occurred at 14^600 'ag/ltc 66 Finney, D. J., Probit. Analysis, 3rd Ed., 1971, Cambridge University Press. * 95% confidance Limits are not defined. Slope; 10.1 I.H;jrg ^Date Isaued; Januagv_28, 1980 iteoowx-fte No. 5"i8B--n8i0ft -- Report by: Approved bys ^ -/.cnv^.g. nV^IL^o^nn^ie-' Hri^nc-ikle Technician .^^J ^ t6----.L____ Gerald^i.* Kenn Chief, Acute Investigat ^^y Sanity, ooes