Document RJOboQRY14ONzzn18RMY8w5kV

AROCLORS METHODOLOGY 1. Does Monsanto have an active analytical program? For how long? Scope? Level of activity? 2. Does Monsanto have methodology? 3. What is the principal of the method? Is the procedure written for issuance to the scientific community? .4 What types of materials can be analyzed with this procedure? 5. What is the accuracy? 6. How are the calculations made for the chromatographic data - for the mass data? 7. Are reference materials available? What isomers? How do we obtain these isomers? What is the purity? ' 8. What is the specificity? Tranrteranor from pesticides? ' How are these handled by the method? What about PCV peaks that have shifted from the standard peaks? What about absolute identifications? Is the GC/Mass proof positive? Does the methodology have other limitations? 9. What is the sensitivity - for GC? - for mass? 10. How much have we exchanged with others on methodology? 11. Do we know of alternate or better methods? 12. Where has Monsanto used this methodology? HONS 035868 (J Environmental Residues AROCLORS Page 2 1. Do river waters and sediment contain PCBs? Which PCBs? Is there evidence for Monsanto's products and what is the source of these products? What do we know about photo-oxidation of DDT or DDE? Can pesticides contain trace contaminants of the PCB type? What about chlorination processes - sewage plants? 2. Does wildlife, including birds and fish, contain PCBs? Which PCBs? 3. What about air - can PCBs be found? .4 Has Monsanto looked at soils for presence of PCBs and pesticides? Process 1. Is methodology being used to monitor plant effluents? Existing Product 1. What does Monsanto know about compositions of Aroclor products, per se7 Can typical product material be provided? How many components have been separated by GC? Can this data be provided? 2. What about biodegradation of Aroclors? Which isomers? Under what type of conditions? Evidence for isomer ratio changes? 3. Thermal stability - is Monsanto looking into this? " Toxicity Tests 1. Does Aroclor build up in tissues? What animals have been studied? Is Monsanto correlating build up with toxicity? Other 1. What do we find regarding Aroclors in dishwater detergents? What about trace toxic impurities of another type in Aroclors, Any knowledge? Has Monsanto looked at milk samples? Findings? 6 9 9 S E 0 SNOW HONS 0 3 5 8 7 0 MONS 0 3 5 8 7 1 MONS 0 3 5 8 7 2 jj 3k__ U j--j--t Aroclor 1221 Aroclor 1242 Aroclor 1248 Gas Chromatographic Conditions lastrument: Hewlett-Packard 5750 Research Chromatogram Detector: High Temperature 63^ Operating Temperatures Inj. Port: 225C Column Oven: 190*C Detector: 290*C Pulse Interval: 150 Attenuation: 8X10 Flow Rates Carrier: 60 ml/min (He) Purge: 120 ul/ain (Ar/CH4-9/l) 1254 Aroclor 1260 o o :) Semi-Continuous Activated Sludge Biodegradation A Aroclor 1242 Mixed Liquor Before Feeding 72 Hour Exposure ' , Aroclor 1242 Mixed Liquor After Feeding II II L_III-----------------------1-----------------------1-----------------------L- 2 4 8 'O !% HONS 035874 ANIMAL LONG TERM STUDIES INTERIM STATUS 2-year rat chronic At 1, 10, 100 ppm Status-9 months 2-year dog chronic Status--9 months . 1242 1254 1260 100 ppm Poss. liver enlargement Males 10 ppm O.K. 100 ppm Poss. Liver enlargement Males 10 ppm O.K. 100 ppm Poss. liver enlargement M&F 10 ppm O.K. 100 ppm O.K. 100 ppm Body wt. dec. 10 ppm O.K. 1M-1F died-- cause unrelated to exp't 100 ppm 1M-1F died refused food-- no other gross abn. 10 ppm possible body wt. effect MONS 035076 E L .. \Z 68 .1 MONS 0 3 5 8 7 8 MQNS 0 3 5 8 7 9 A - ii HONS 0 3 5 8 8 0 jnitno v / n$b c t tCihES. a qS BHC (BETH BHC BHC. ~ /VSECTHZf OE 1-} gprACHLofc. HePTACHLoit e^oK\bt ALbRlM MONS 035881 cHQ?i CJL 0 bbT" ODD l^^^ * HONS 035882