Document RJJRNpoVk0j3nEjMEj306oEzn

MICROBICS MICROTOX@ TOXICITY TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-787 or FC787X. (1-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 101. The testsample isFC787. Currentinformationindicatesitisa mixtureof0.73% PFOS, 10% diethyleneglycolmonobutyl ether,79.74% water,5% ethyleneglycol,1.47% propane Sultonefoamer,0.73% sodium octylsulfate0,.73% sodium decyl sulfate0,.75% xanthan gum, 0.75% magnesium sulfate0,.10% N-(3chloroallyl)hexaminiucmhloridea,nd 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: MicrobicsMicrotox@"BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1990. Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions: Diluent: Microbics2% NaC[ solution Test temperature: Not noted. Stock and testsolutionpreparation:A primary2000 mg/L stock solutionwas prepared by dissolvin2g00 mg oftestmaterialin100 mL ofMilliporMeilli_QTM water. The pH ofthe stocksolutionwas then adjustedfrom 5.5to6.8 usingdiluteNAOH. An osmotic adjustment was made using200 mg NaCl dissolvedin10 mL stocksolution.All testsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlighltoss RESULTS NominalconcentrationBsl:ankcontro1l2,5,250,500,and 1000mg/L Element value: 5-minuteEC50 = 330 (300 - 360) mg/L 15-minuteEC50 = 250 (240 - 270) mg/L 30-minuteEC50 = 210 (200 - 230) mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 30-minuteEC50 forPhotobacteriumphosphoreum was determinedto be 210 mg/L witha 95% confidenceintervaolf 200 to230 mg/L. Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133 DATA QUALITY ReliabilitKyl:imischranking2. The study meets the criterifaorquality testing.However, thesample puritywas not propedy characterizedand the studylacksanalyticaclonfirmationofthe amount offluorochemicalproportion inthe solution. REFERENCES Thisstudywas conducted by the 3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number H1608, 1990. OTHER Last changed: 6/28/00. gnv Aittro-VoA -79 7 LR Resul+s + /e)I 500 /000 Per-een t Ia%I s-s 5-rnin- L-*P. 15- rnin.Efif!. 3o-rnin. Etip. 3 ;z 3q ql 51 103 70 76 7 (,1 11-50 330 (300-3bo) R670 (aqo - a 70) @z10 (;too - ;?so ENVIRONMENTAL LABORATORY MICROBICS MICROTOXR SYSTEM RAW DATA LR pq) TEST SUBSTANCE: TEST DATE ANALYST: SAMPLE CUVETTE C- 7-9 -7 L04 @ot FINAL ASSAY CONCENTRATION INITIAL READITIG 1(0) FINAL REAL)ING5 5-MIN. 15-MIN. 30-miti. 1(5) 1(15) 1(30) BI ci BZ C2 83 C3 .B4 C4 B5 C5 'Te-5,iyellowti501A BLANK CONTROL DUPLICATE 1;715 vp1 5-*3 q #5 3 Cl 35- #5- 3& 3,3 50 Dvpl@,co-4e 5-00 >vpl;c&4C 3,q .@l a:Z q3 317 @s IRq /01 :2q 17 )3 97 oZq 17 /DOC) qs 16 10 Cl9 /,57 7 S+or-Y- 20orAId leornt AA I)I-LI -10PA (,-SW141%eLtlv4eNmo44 t:> Tnt*+iA,L A),act golv+ioh ontev)+railC"% ftxnfionea lhtrc>4LIc,eL lri=.fke %hVo 4ke neA+ +wo rows. +k -C,ot aosnss vweie P% :'----787 5-MI@j EXF'OSURE !ICROTOX(t-) DATA StiEET CJAE( r-Alr( cotic. to/It 45. i:v 125. 1)01) 9 t:) 45. -45f).1)00 4 25().ci(,)o 9 7.6. 4-w 5 f)f) I()1. '-74. t: 51)(1.ooo 0/ --')4. 7 % @(*.(10(.'i() 9e.o/ @j % 1 'ot'i).). 'oi)(--) 98.4)/ 15. eLANtr Do/Bt= 93 / bt.) ELAPIII F<ATID= ('.@.645'-, G-OBS 419 4-.).746 C,.667 1.715 1.608 2.952 15 @-zr-5(.*j ;L, 94;p426 '-"27 300 SLOPE T#3 @@57.799 - 31.0 ) rr),l 95*:.9 INTERCEFT +5.7967 G-EE-T 17:76 7 0. 3!@7 0.75'@@' 1).75--., 1.543 1.54@@ 3.lb5 7L..16t, Li9a,kC+L)osse.) 3u A5 q -Ij &b3@3&3 76- 76a 7& 6-06S --.@ (6-obs, + )( job #0 RES. PROE4. :).1'-17 76 0.146 100 ().4-91 @9 4 ().096 44 5 0.096 43 6 0.092 40 7 0.132 e2 a 0.139 91 - ----------------- --------------- ---------------- i L 0 1> ----7-< Fj ------- C3 1A -6 --- m m A > s --- ------------ --------------- 4 5 LOS CONC. 6 7 MICROTOX is a Registered Trade Marlt of Microbics Corporatiori. p%3 r-c--,97 15-MIN EXPOSURE MICROTOX(R) DATA SHEET 6/16/90 SAB CONC. IC/It G-OE-IS 1:.,5. 4 99.o/ 7,9.() 72.0/ '26.1) 93.()/ 2EI. 11)1.4)/ 17.0 97. (:)/17.0 90. o / 1,). ':).447 C@.472 1.()17 C).693 2.7,86 25'-@' 4.5135 4.585 5 G-EST 0.45b 0.995 o. 1?95 1 6e 2.169 4. 7--'f, 4. 7'-)!@, 6 q7 70 b9 192, S.2 3 x ICkD 70 s;z_ c-7C 5,:) --@51.L@71 2--.7.9G7 TO 265.931 g,5c)(.2qo - ;Z7o) rh,%Ii- R =C).99784 SLOP,E = C).Be5g TNTERCEPT +5.5277 ,:2 ----------------V ---------------v ---------------- RES. PROF,,. I t)e6 1f)() C)O 94 C)55 41 --- 8-i 4 t).058 5 ().058 6 0.057 46 46 43 1> ----- L 0 <6 7 o.087, 94 #1 5--- (3 0 0'-T 94 -6- 6 A m m A 0> s -------------------------------- 4 5 LOG CONC.- 6 MICROTOX is a Registered Trade Mark of Microbics Corporation. q ilCF<OTOX@r@) D@)TA IZ@IEET FC-707 TIO-MINIJTE EXPOSURE a.;16 @oi) SAD F'AIR co@1C. Io/It 250. ,@0() 22.( 4 250. Ot:),:) 24.(.) 5 500. 1(,)1.0/1 (-@ lb 50o.O()() 97.(.)/1 7 %loo4).,)(*.@O El % I 00o. (@4*.)O G-OBS 1 521 t'.515 1 1.083 @-'.177 3.012 5.586 6.5Z,7 910 G-EST L,* LOS& 3m 0.524 3 3q 0.5"@4 1. 2q)7 1. 2i:@7 37(o 5,@7,3 2.78() 2. 78i'l 713 7b 75 6. 41.15 135 6.4o5 87 SLANT:' Eic)/Bt= 93 -L -)N'-,R:ATIO= 0.57-76 x too =-C 5t') "-@'14.76E3 =o.99597 FLOPE 1 ?7 . 7 T0 4- 1-n,%IL IIATERCEPT +5.3694b RES. 0.116 0.117 0.075 4 080 5 0.0(31 1) 0.079 7 0.108 E3 0.117 PROB. 9c? lo(-) 41 46 47 45 85 100 ----------------v ---------------v ----------------8-- i -7-: L 5---- 0 1 -6--- <6 G A m m A > <s --------------- --------------- ---------------- 4 5 LOS CONC. 6 7 miCROTOX is a Reqistered Trade Mark o+ Microbics Cor,poration.