Document RJ5R2BrxDbm716N2bLVvRRwqX

ar. * a 6 _ o6gs TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:_________________________________________________________ Method: NS-EN ISO 10253, 1998: W ater quality - Marine algal growth inhibition test Type: Acute Static GLP: No Year completed: 2000 Species: Skeletonema costatum Source: Strain NIVA BAC1, stock culture maintained at RF - Rogaland Research, Stavanger, Norway. Element basis: Estimate biomass measuring fluorescence. Exposure period: 72 hours Statistical Methods: Growth rates by Log linear regression on biomass (fluorescence) vs. time, as described in NS-EN ISO 10253. Calculation of NOEC done by single factor ANOVA followed by Bonferronis procedure. Analytical monitoring: pH Test Conditions: Algal Nutrient Medium: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway, enriched with nutrients according to ISO 10253 and recommendations from the Paris Commission. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.3. Stock and test solution preparation: A 511.2 mg/L primary stock solution was prepared by diluting 0.5112 grams of test substance in 1 liter of growth 000197 medium. Aliquots were then added directly to growth medium in test vessels to create test solutions. Exposure vessels: 100 ml flat-bottomed flasks containing 50 ml test solution. Agitation: Shaken continuously on an orbital shaker. Number of replicates: 3 Initial cell loading: 2.0 x 103 cells/mL Number of concentrations: seven plus a blank control Lighting: Continuous fluorescent lighting at >50 pE/m2 sek. Water Chemistry: pH range (0-96 hours): 8.3 - 8.4 (control exposure) 8.2 - 8.4 (100 mg/L exposure) Test temperature range: 20 2 C RESULTS___________________________ _____________________________ Nominal concentrations: Bk control, 0.1, 0.32,1, 3.2,10, 32, and 100 mg/L. Algal Growth Response ErCso values (based on growth rate) 72-hour ErCso = ~50 mg/L 72-hour NOEC = 10 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not conducted. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance exhibits a 72-hour ErCso growth rate value of ~50 mg/L. The 72-hour No Observed Effect Concentration (NOEC) is 10 mg/L for growth rate. Validity criteria fulfilled. Control cell density increased by a factor of more than 16 in the test period and reference substance was within normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ 000198 Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, purity of the test substance was not property characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-1, 2000. O T H E R ___________________________________________________________ Last changed: 6/26/00 000199 Lab.ref.no.: 99312-1 Page 1 o f 3 1 lOl NORSK AKKREDITERING ROGALAND RESEARCH "FC-201 AF Light Water Brand AFFF" Toxicity tests Report RF-2000/001 Lab. ref. no.: 99312-1 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 /0 5 .0 1 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-201 AF Light Water Brand AFFF': Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC 48 h LCgj 10 d LCju (mg/i) : -5 0 (mg/!) : 34 (mg/kg dry weight) : >3020 The results relate to the product as supplied by Novatech. Key-words: ---- Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this docum ent may be reproduced in any form without permission in writing by RF Rogaland Research O . jS tu d y lire c to r / Inger Lisa Andersen for RF - Rogaland Research Kre Netland 000200 Lab.ref.no.: 99312-1 Page 2 o f 31 CONTENTS: 1. Preface.......................................................................................................................................................3 2. Test for toxicity with Skeletonema costatum.........................................................................4 3. Test for toxicity with Acartu tonsa.......................................................................................... 10 4. Test for toxicity w rra Corophium volutator......................................................................... 15 5. Data sheet(s) ......................................................................................................................................... 20 000201 Lab.ref.no.: 99312-1 Page 3 o f 31 PREFACE: Ecotoxicological tests were performed on "FC-201 AF Light Water BrandAFFF*in agreement with Novatech. The sample is a concentrate that normally will be used in a 1% solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 / SF T1998). The project was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Miljelab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to theproduct as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and workingproceduresfo r all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in interlaboratory comparisons, and the use o f certified reference materials and secondary reference materials. The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without thepermission in writing by RF - Miljolab. 000202 Lab.ref.no.: 99312-1 Page 4 o f 31 T est fo r T o xicity Marine alga: Skeletonema costatum 000203 Lab.ref.no.: 99312-1 Page 5 o f 31 TEST REPORT GROWTH INHIBITION TEST WITH THE MARINE a l g a Skeletonema costatum Irli ti NORSK AKKREDITERING Nr. POI* RESEARCH TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The 72 hours EC50 was calculated to ~50 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NS-EN ISO 10253,1998: Water quality - Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricomutum. TEST ORGANISM: skeletonema costatum, strain NIVA BAC 1. A stock culture of the test organism is kept at RF - Rogaland Research. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the growth medium. The water was filtered through a GF/C filter, and briefly heated to about 75C prior to use. The seawater was enriched with nutrients according to ISO 10253, and recommendations from the Paris Commission. Test solutions were prepared from a stock solution. A stock solution o f 511.2 mg/1 was prepared by stirring 0.5112 grams of the product in 1 litre of growth medium. Other concentrations were prepared from this stock solution. INCUBATION: Test cultures o f 50 ml were incubated in 100 ml flat-bottomed flasks on an orbital shaker. 3 replicates for each concentration. Test cultures were inocu lated to about 2000 cells/ml from an exponentially growing inoculum culture. The test was run under continuous light (> 50 pE/nr-sek) from fluorescent tubes o f universal natural white type, at a temperature o f 20 2C. The test was run for about 72 hours, and subsamples of 5 ml were taken from the test flasks each day for growth measurements. Algal growth was measured by fluorescence. pH measurements were performed as indicated on data sheet. 000204 Lab.ref.no.: 99312-1 Page 6 o f 3 1 DATA ANALYSIS: CALCULATIONS: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described in NS-EN ISO 10253. ErCso: The inhibitory effect on the growth of Skeletonema costatum is expressed as the concentration of a product giving 50% reduction of growth rate (ErCso). This effect concentration was calculated by interpolation between 32mg/l and 100mg/l.. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonierronis procedure for comparing each concentration mean v/ith the control mean. TEST RESULTS: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance "FC-201 AF Light Water Brand AFFF" ErCio _ ErCso ~50 EfCoo _ NOEC: 10 Values indicate concentrations giving 10%. 50% and 90% inhibition o f growth rate. Units in mg/l. Values are calculated by interpolation. NOEC are calculated by using the computer programme GraphPad InStat 3.0. COMMENTS: The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor of more than 16 in the test period (corresponding to a growth rate of more than 0,9 d'1), the control pH did not vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-1 20.09.99 04-07.10.99 05.01.2000 Elin Horve Engineer Analytical technician ge Molversmyr ' Senior Research Scientist Quality Assurance Enclosed: Data sheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljolab. 000205 Lab.ref.no.: 99312-1 Page 7 o f 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 Start date: 04.10.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence: values in fluorescence units Time: 04.10.99 05.10.99 06.10.99 07.10.99 Concentration 14:05 09:25 10:30 10:40 Growth pH pH rate Corre- at start at end day -1 lation of test of test 0 0.11 0.59 9.2 56 2.18 0.997 0 0.15 0.63 7.9 59 2.09 0.999 0 0.13 0.62 9 54 2.11 0.998 8.3 8.4 0 0.14 0.63 6.4 47 2.04 1.000 0 0.14 0.62 6.3 48 2.04 1.000 0 0.12 0.71 7.1 55 2.14 1.000 0.1 0.16 0.74 7.2 60 2.07 1.000 0.1 0.13 0.61 5.5 55 2.12 0.999 8.4 8.5 0.1 0.14 0.55 8.3 64 2.14 0.998 0.32 0.32 0.32 1 1 1 3.2 3.2 3.2 10 10 10 32 32 32 0.14 0.74 0.14 0.68 0.14 0.68 8.7 8.6 9.2 58 63 62 0.14 0.83 0.15 0.88 0.13 0.93 8.2 9 8.9 59 61 61 0.14 0.86 0.16 0.76 0.15 0.63 9.2 8.9 7.3 67 73 63 0.16 ' 0.15 0.16 0.45 0.46 0.46 5.5 5.2 6.2 57 52 49 0.14 0.15 0.13 0.4 0.3 0.4 3.4 25.5 2.7 17 4.4 33 2.11 0.999 2.14 0.999 2.13 0.999 2.11 1.000 2.10 0.999 2.15 0.999 2.16 0.999 2.14 0.999 2.11 0.999 2.06 0.994 2.05 0.996 2.00 0.995 1.82 0.996 1.66 0.991 1.94 0.997 8.4 8.4 8.4 8.4 8.4 8.4 8.4 8.5 8.5 8.4 100 0.15 0 0 0 0.00 100 0.18 0.01 0.03 0 0.00 100 0.13 0 0 0 0.00 8.4 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.14 0.47 1.85 1.5 mg/l 0.16 0.43 1.45 1.5 mo/l 0.16 0.45 1.45 4.2 3.3 2.9 1.19 0.992 1.06 0.996 1.01 0.992 8.3 8.2 Mean values: 0 0.1 0.32 1 3.2 10 32 hnn 0.13 0.6 8 53 0.14 0.6 7 60 0.14 0.7 9 61 0.14 0.9 9 60 0.15 0.8 8 68 0.16 0.5 6 53 0.14 0.4 4 25 n 4cr n, r\ A r\ 2.10 0.999 2.11 0.999 2.13 0.999 2.12 0.999 2.14 1.000 2.04 0.995 1.80 r\r\ 0.995 Sr'""00206 Lab. ref.no.: 99312-1 Page 8 o f 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 5 OS <33. 1 O C 1 0 0 m g /l 24 48 72 test duration (hours) 000207 Lab.ref.no.: 99312-1 Page 9 o f 31 Inhibition of the growth of Skeletonema costatum with: mg/1 ("FC-201 AF Light Water Brand AFFF") Experimental data: Concentration in Growth rate "FC-201 AF Light Water Brand AFFF" Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.10 0.10 0.10 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 2.18 2.09 2.11 2.04 2.04 2.14 2.10 2.07 2.12 2.14 2.11 2.14 2.13 2.11 2.10 2.15 2.16 2.14 2.11 2.06 2.05 2.00 1.82 1.66 1.94 0.00 0.00 0.00 Inhibition in percent - ~ " 0 1.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.9 2.4 4.8 13 3 ? 21.0 X /4 7.6 100.0 100.0 100.0 000208 Lab.ref.no.: 99312-1 Page 10 o f 31 T est fo r T oxicity Marine herbivore: Acartia toma 000209 Lab.rej.no.: 99312-1 Page 11 o f 31 TEST REPORT ACUTE TOXICITY TEST WITH THE MARINE COPEPOD Acartia tOtlSCl (0) NORSK AKKREDITERING Nr. POI ROGALAND RESEARCH TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The 48 hours LC50 was calculated to 34 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ISO/DIS 14669 - 1997: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). TEST ORGANISM: Acartia tonsa (Dana), supplied by the Marine Biological Laboratory, University of Copenhagen. CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance with the "Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. Preparatio ns: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the dilution water. The water was filtered through a GF/C filter prior to use. Test solutions were prepared from a stock solution. A stock solution of 999.4 mg/1 was prepared by stirring 0.4997 grams of the product in 0.5 litre of dilution water. Other concentrations were prepared from this stock solution. INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml of test solution and 5 animals, 4 replicates for each concentration. The vials were kept under a 16h/8h light/dark photoperiod at a temperature of 20 1 C. pH and oxygen measurements were performed as indicated on the data sheet. L-. 000210 .J L -- rm i Cl-- Klnrwnv Tel.: + 47 51 8 7 5 0 00. Fax: + 47 51 8 7 5 2 00. Enterprise number.: 950884800. Lab.ref.no.: 99312-1 Page 12 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Acartia tonsa is expressed as the concentration of a product lethal to 50% of the test animals (LC50). The combined mortality per test concentration were used for calculation. The 24 hours and 48 hours LC50 values and the 95% confidence limit were calculated by using the standard procedure PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. T e s t RESULTS: Table 1. LC and NOEC values obtained witb:"FC-201 AF Light Water Brand AFFF" Acartia tonsa 24 hours 48 hours LCio LCjo LC90 20 (10-2 9 ) 52 (37-71) 130 (92-260) 12 ( - 29) 34 ( - ) 93 (39- ) NOEC: 32 1 0 Values indicate 10%, 50% and 90% lethality after 24 and 48 hours. Units in mg/l. Values are calculated by PROBIT analysis, and 95% confidence intervals are indicated. NOEC are calculated by the computerprogramme GraphPad InStat 3.0. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-1 20.09.99 06-08.10.99 05.01.2000 Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance Enclosed: Datasheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljelab. m 000211 Lab.ref.no.: 99312-1 Page 13 o f 31 PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 06.10.99 Number of dead Concentration (mg/l) Control 10 32 100 320 999 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 555 30 5 5 5 5 55 20 5 5 55 20 5 555 20 5 5 55 20 5 555 20 5 NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE B DF Total 0 00 0 01 -0 0 0 1 101 2 0 01 0 1 11 10 3 0 20 121 1 31 -5 4 44 5 5 55 17 5 20 5 55 5 555 20 5 20 5 55 555 5 20 5 20 - Reference substance (3,5-dichlorophenol): 1.0 mg/l 555 0 20 254 30 555 100 3 130 The result with the reference substance is within the range normally found. 15 I I 000212 Lab.ref.no.: 99312-1 Page 14 o f 31 Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: 99312-1 06.10.1999 Measurements of oxygen and pH. Start 24 hours 4i hours Concentration (mg/i) Oxygen (mg/l) pH Oxygen pH Oxygen (mg/l) (mg/l) Control 7.6 8.1 7.2 10 7.6 8.1 7.0 32 8.1 8.1 6.5 100 7.7 8.1 5.6 320 7.7 8.1 6.6 8.1 999 7.5 8.1 6.5 8.1 pH 8.1 8.1 8.1 8.0 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.7 8.1 7.3 8.2 000213 Lab.ref.no.: 99312,1 Page 15 o f 31 T est fo r to xicity Marine Amphipode: Corophium volutator 000214 Lab.ref.no.: 99312-1 Page 16 o f 31 TEST REPORT ACUTE TOXICITY SEDIMENT TEST WITH THE MARINE AMPHIPOD CorophilWl V O lu td tO Y fQl NORSK AKKREDITERING Nr. POIS ROGAIAND RESEARCH TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The lOd LC50 was calculated to >3020 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E1367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount o f natural seaweed was used for feeding. Pr e p a r a t io n : Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside Stavanger was used for dilution water. Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grammes of damp sediment in a plastic bucket. 200 ml of seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of the damp sediment was analysed for water content, and all concentrations are calculated as mg kg'1sediment dry weight. In c u b a t io n : The test was run in glass containers (600 ml) each containing 200 g of test sediment, app. 400 ml of seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a temperature o f 15 1C. pH and oxygen measurements were performed as indicated on the data sheet. ! 000215 IL Professor Olav Hanssensvei 15, P.O. Box 2503 Ullandhaug, 4091 Stavanger, Norway. Tel.: +47 51 8 7 5 0 00. Fax: + 47 51 8 7 5 2 00. Enterprise number.: 950884800. ocptscm - TtwrrmiliUnsnt 55..4008Bergen, Norway. Tel.: +47 55 54 38 50. Fax: +47 55 54 38 60 Lab.ref.no.: 99312-1 Page 17 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen tration of a product lethal to 50% of the test animals (LC50). The combined mortality per test concentration were used for calculation. The 10 day LC50 value and the 95% confidence limit are calculated by using the standard PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% o f the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with: "FC-201 AF Light Water Brand AFFF' Corophium volutator 1 0 days LCio 800 LC50 >3020 LC90 >3020 NOEC: 495 Values indicate 10%, 50% and 90% lethality after 10 days. Units in mg kg'1sediment dry weight. Values are calculated by PROBIT analysis. NOEC are calculated by the computer programme GraphPad InStat 3.0. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the percentage mortality in the controls were less than 15% and a test with Fluorantene (reference) was in the range normally found, but the dissolved oxygen concentration was less than recommended (60 %) in one o f the test concentrations (3020 mg kg"1 dry weight sediment) after five days and in one o f the replicates after ten days. The sediment used is found to be within accepted limits for organic content but the silt fraction is recommended higher. Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0 .8 Lab. ref. no.: Sample received.: Test period: Report date: 99312-1 20.09.99 03-13.12.99 05.01.2000 Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance Enclosed: Data sheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljelab. 000216 Lab.ref.no.: 99312-1 Page IS o f 31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 03.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 92.4 10 10 10 30 10 10 10 495 30 1030 10 10 10 30 3020 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE BDF Total ACE B DF Total 0 00 0 00 0 100 1 0 11 2 121 4 1 22 5 - Reference substance (fluoranthene): 20.3 10 10 10 30 322 The result with the reference substance Is within the range normally found. 7I 000217 Lab.ref.no.: 99312-1 Page 19 o f 31 Test substance: "FC-201 AF Light W ater Brand AFFF" Lab. ref.: Start date: 99312-1 03.12.99 Measurements of oxygen and pH. Start 5 days Concentration Oxygen (mg/kg dry weight) (%) pH Oxygen {%) pH Control 94 8.1 91 8.1 92.4 94 8.1 76 8.1 495 94 8.1 91 8.1 1030 95 8.1 87 8.0 3020 97 8.1 49 7.8 10 days Oxygen (%) pH 91 8.0 88 8.1 89 8.1 89 8.1 77 8.0 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 000218 Lab.ref.no.: 99312-1 Page 20 o f 31 D ata Sheets 000219 Lab.ref.no.: 99312-1 rage i oj si HMS-DATA3LAD {Heise- xaaaaaasaaaiasssBsssaaaaaasasaaaaaiaaaataaia og Sikkerhetsdatablad) Dokumentr.: Ersratcer : 10-4145-6 Utgitt : 11/11/98 Versjon - : 06.01 03/11/93 Skrevet ut : 04/01/99 Side 1 av ll KMS-dacabiadet er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIES JON AV KJEMIKALIET OG ANSVARLIG FIRMA. Emprter: 3M Norge A/S Har.delsr.avn: FC-602 LIGHT WATER 3RAND ATC PLUS Deklarasjonsr.ummer, Procuktregisteret: ?-nr.: 035337. Telefon, Gifrinformasjonssantralen: 22 59 13 00 2 OPPLYSNINGER OM KJEMISK SAMMENSETNING. Informas io n on en k eltkorr.D onenter, faresymbol(er) og r a s ik o s e t m n g e r STOFFNAVN CAS-nr VEKT-PROSENT VANN 1,2-ETANDIOL Faresymboler/risikosetninger: Xn; 2-(2-BUTOKSYETOKSY)-ETANOL / (DIETYLENGLYXOLMONOBUTYLETER) Faresymboler/risikosetninger: Xi; FLUORALKYL OVERFLATEAXTIVE STOFFER SYNTETISKE OVERFLATEAXTIVE STOFFER MAGNESIUMSULFAT TYXNERE RESTER AV ORGANISEE FLUORXJEMIKALIER 7732-18-5 107-21-1 22 112-34-5 36 Trade Secret Trade Secret 7487-83-9 Trade Secret Trade Secret 58 - 63 20 * 12 1-5 1- 5 =i 0.5 - 1.5 Ikke kjent II ! ! 0002^0 Lab.ref.no.: 99312-1 Page 22 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 3 VIXTIGSTE FAREMOMENTSX. Vikrigsze skadevirkninger Helsefare: Kan irritiere oynene, huden og luftveiene. Sikkerhet: Miljafare : Spesiaiavfall. 4 FGRSTEHJELPSTILTAK. Tilrak evr.e: Skyll srraks oyner.e med rikelige mengder var.n. Kontakt lege unticdelbarr. Tiltak hud: Skyll huden med rikalige mengder vann. Evis irritasjonen vedvarer, kor.rakt lege. Tilrak innancing: Evis symptomer oppstir, bring vedkommer.de bort fra eksponeringskilden. Sorg for frisk lut. Hvis symptomene vedvarer, kontakr lege. Tilrak svelging: Kontakt lege umidcelbart. Fremkall brekninger kun i samrid med medisinsk personell. Gi aldri noe gjen.nom mu.nr.en til en bevisstlos person. . 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsraiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: Lab.ref.no.: 99312-1 Page 23 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS Forbrer.nir.gsprodukzer ved brann Prosedyrer ved slukking av brann: I i 6 TILTAX VED UTILSIXTET UTSLIPP. Personlige vernetilcak: Se vernetiltak under andre punkter i dette EMS-databladet. iltak ved utilsiktez utslipp: Ventiler omrcez. Spill avgrenses umiddelbartu Dekk til med et absorberende materiale. Spill samles opp umidcelbart. Rengjar omrdet med vann. Piasseres i en egnet beholder med lokk. Fjerr.ing av k jemikalieavf all : Se punkz 13, Fjernir.g av k jemikalieavf all. ii ! I 7 hAn DTERING OC- 0PP3VARIMC-. j ___________ '______________________________________________._______________ I Betingelser for sikker cppbevaring: Mi ikke lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader Celsius. Lagres ved temperaturer over 0 grader Celsius. Hold beholceren tett lukket nr produktet ikke er i bruk. j Uforenelige materialer: Ventilasjon: Oppbevar embaliasjen pi et godt ventilert sted. Sikkerhetstiltak for i forhindre brann: i I I i Sikkerhetstiltak for i forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for forhindre statisk elektrisitet iI i i r Forholdsregler ved bruk: Hold emballasjer. tett lukket. Rayking forbudt: Under bruk av produktet kan rayking forirsake forurensning av tobakken og/eller rayken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. Lab.ref.no.: 99312-1 Page 24 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 3 e :<s ?o n i r :::g s x o n t r o l l c g p e r s o m l i g v e r n e u t s t y r . Oyeverr. : Unngi i fa damp, spray eller take i aynene. Bruk tette veraebriller med ventiler. Hindverr.: 3ruk egnede vernehansker av hensiktsmessig materiale ved hindtaring av produktet. Vernehansker av folgende materiale(r) ancefales: Butylgumni. Ar.net hudvern enn hindvern: Unngi hudkontakt. Andedrettsvern: Unngi ir.ninding av luftbiret materiale. Basert pi konsentrasjonen av leftiorure.nsringer i arbeidsatmosfaren velges er. av folgende type(r) godkjer.t ir.dedrettsvern: halvmaske med filter mot orgar.iske damper og vaskeformige partikler: A/P2SL. Svelcing: Det mi ikke spises, drikkes eller raykes under arbeid med Produkten. Vask utsatte omrider grundig med sipe og vann. Vask herders enter hirctering og far spising. Anbefalt ventilasjon: Kvis utsugningsventilasjon ikke er tilstrekkeiig, mi egnet godkjer.t indedrettsvern benyttes. Sarg for tilstrekkeiig ventilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger: Ingen adm. norm. 1,2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stav) 8-timers verdi mg/m3: 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2-(2-BUTOKSYSTOKSY)-ETANOL / (DIETYLENGLYKOLMONOBUTYLSTER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). FLUORALKYL OVERFLATEAKTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. SYNTETISXE OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. Lab.ref.no.: 99312-1 s Page 25 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS ----- rwjnss- i'-TAT-- (1ivrr-uj-'j ; ..... -- Anmerkninger: Ingen adm. norm. TYXNERE (Trade Secret) Anmerkninger: Ingen adm. norm. a RESTER AV ORGANISXE FLUORXJEMIXALIER {Trade Secret) 8-rimers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. 9 FYSI3XE OG XJSMISXE EGENSKAPER. Tiiscandsform, farge, lukt: Ugjennomsiktig, ravfarvet, viskos vaske. pH : ca. 8.5 Kokepur.kt/-intervall : ca. 93.00 C (3egynnende) SiteItepurkt/-interval I : Ikke relevant Flammepunkt: Selvantennelighet: Sksplosjonsgrenser, nedre: Ingen (Setaflash CC) Ikke relevant Ikke relevant Eksplosjonsgrenser, ovre: Ikke relevant Damptrykk: ca. 16 mmHg {Kalkulert v/20 C) Loselighet i vann: 31andbar. Spesifikk vekt: ca. 1.06 (vann=l) Damprert'net: ca. 0.85 (luft=l) (Kalkulert v/20 C) Flyktige organiske forbindelser (VOC) : = 339 g/1 SCAQMD (Kalkulert v/20 C) Fordampningshastighet: < 1 (butylacetat-1) Viskositet: Ikke kjent 10 STA3ILITET OG REAXTIVITET. 0002 Lab.ref.no.: 99312-1 Page 26 oj 31 FC-602 LIGHT WATER BRANO ATC PLUS Forholc som skal unngis : Materialer som ska! unngs: Ikke kjer.t. Fariige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eiler partikler. Termisk dekomponering av bruksferdig blanding forve.ntes ikke representere noen risiko. Stabilirei og reakrivitet: Stabilt. Farlig polymerisasjon vii ikke forekomme. 11 OPPLYSNINGER OM KSLSEFARE. Effekter ayr.e: Mild aveirritasjon: symptomer kan vare redhet, hevelse, smerter og Effekter hud: Mild hucirritasjon (etter langvarig eller gjentatt kontakt): symptomer kar. vare radhet, hevelse og kloe. Effekter ir.r.nding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fare til: i j i j j Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, savnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Svelging kan fare til: .Pvirkning av nyrene: symptomer kan vare redusert urinmengde, blod i urinen og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er 00 Lab.ref.no.: 99312-1 Page 27 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS oppkast.): svmpzomer kan vare hosting, pustevansker, gisping, hoste opp blod og lungebetennelse som kan vare livstruende. Informasjon on allergi, overfolsomhet : " Kreizfremkalier.de egenskaper : Arveszoffskadelige egenskaper : Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dezze produkzez inneholder en eller flere organiske iiuorkjemikaiier, som muligens kan absorberes og lagres i kroppen over lengra tid, er.tan som utgangsmolekyiet eller som r.edbrytningsszof fer (mezabolitter), og kan akkumuleres ved gjer.tatte ekspor.eringer. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befclkningen og underpopulasjoner som arbeidere, har varz p.ublisert siden 1970 arene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige efSekter. Et resyrrT vedrarende produktets giftighet er tilgjengelig p forespersel. 12 OPPLYSNINGZR OM MILJOFARE. Persistens/3iologisk nedbrytbarhet : Dette produktez inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljaet (persistente) . akotoksikologiske data: Et 3M miljo-produkzdatablad er tilgjengelig pk foresparsel. 'Andre skadevirkninger, milja: Nedbrytbarhet: COD (Chemical Oxygen Demand): 0,552 g/g; BOD 5 dagers (Biochemical Oxygen Demand): 0,254 g/g; BOD 20 dagers: 0,422 g/g; 20 dagers BOD/COD: 0,76. Akvatisk giftighez: ______ 96 timers LCSO for fisk (Fathead minnow (Pimeohales promelas)): > Lab.ref.no.: 99312-1 Page 28 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 1000 mg/1 ; 43 timers EC50 for daphnia (Daphnia magna): 1000 mg/1; 5 minutcers og 15 mir.utters EC50, Photobacterium phosphoreum (Microrox System): henholdsvis 210 mg/1 og 220 mg/1. * Sorg for avf ailshr.dcering ihr. gjeldende forskrifrer. 13 FJERNING AV XJEMIKALIEAVFALL. Me roder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mcttaks- eller behar.dlingsanlegg for.'spesialavfall. Anbeiaide avfallsgrupper: HOVEDKATSGORI 07: AVFALL FRA ORGANISKE PROSESSER. . UNDERXATSGORI 07 06: Avfall fra P3DB av fettstoffer, slpe, rengjoringsmidier, desinfeksjonsmidler og kosmetikk. EAX-koce 07 06 03: Halogenerte organiske losemidier, vaskevasker og mocerlut. Avfallsroffnummer: 7151 Organisk avfall med halogen. Spesielle komponenter: Ecylenglykol, fluorforbindelser. 14 OPP1YSNINGER OM TRANSPORT. ADR-klasse: siffer: bokstav: margnummer: CEcIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: IMDG-side: EMS-nr.: MF AG: 000227 Lab.ref.no.: 99312-1 Page 29 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS Ovrige ooplysninger: Ikke transportfarlic gods. 15 OPrLYSNINGER OM LOVER OG FORSKR2FTER. Merkeerikett: Symbol!e r ) : KELSESXADELIG (Xn) Ir.r.eholder : Etylenglykol 18-22 % 2-(3utoksyetoksy)-etanol 10-15 % R-seeringer : Farlig ved svelging. Kan ere aynene, huden og luftveiene. Ur.ngi innlnding av damp/spravtet4ke. Unr.g4 kontakt med ayneae og iangvarig kontakr med huden. Mi bare anvendes p4 godt ventilerte sreder. F k r man sto'ffet i ayr.ene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask med sape og vann. Ved svelging, kontakt lege omgence og vis denne beholderen eller etiketten. Oppbevares utilgjengelig for barn. V Korr, yrkeshyg. luftbehov: 0-30 ml/l Ovrige ooplysninger om merkeetiketten: Ovennevnte merking er b a s e m pi tidligera merkeforskrifter og vil vare 4 firme p4 produktet er. tid fremover. Merkingen vil imidlertid e'rstattes av folgende merking, som er basert p4 reviderte merkeforskrifrer av januar 1998. Symbol: Ingen Ir.neholder: 1,2-etandiol, 2-(2-butoksyetoksy)-etanol Kan irritere aynene, huden og luftveiene. M4 bare anvendes p4 godt ventilerte steder. Unng4 inn4nding av damp/spraytet4ke. Unng4 kontakt med aynene og langvarig kontakt med huden. F4r man Stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt Lege. Etter hudkontakt, vask med s4pe og vann. M4 ikke' svelges. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. Oppbevares utilgjengelig for barn. YL-gruppe: 00 000228 Lab.ref.no.: 99312-1 Page 30 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS I ! Saesielle Lover og bestennelser: { I 1. Forskrift on utarbeidelse og distribusjor. av Heise-, Mil jo- og ! Sikkerhetsdatablad for farlige kjemikalier. : 2. Forskrift oc kiassifisering og merking av farlige kjemikalier. 3. Forskrift ora kiassifisering, merking m.v. av Stoff som kan medfore miljafare. 4. Forskrift on stoffkartotek. 5. Forskrift on spesiaiavfall. 6. Etc. ! t 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUKERNES SIKKERHET OG HELSE. Anvendelsesomrder: Filmdannende skunkonsentrat for slukking av brann i hydrokarboner og polare lasenidler eller vasker. Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. Arsak til oppdatering: HeLe catabladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egr.e opplvsninger: Informas jonen pi dette .'HMS-databladet er i henhold til vre nvarende opplvsninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medfarer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. I i I i i ! Ii j ! ! 00 Lab.ref.no.: 99312-1 Page 31 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS FOLGENDE PRODUXTER DEXXES AV DETTE HMS-DATA3LADET: 3M ID-r.r XF-0008-0221-5 3M AFFF/ATC FC-602 Brannsluknin gsskura Produksjon 3M ID-r.r XF-0008-0100-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602,1000 L 3M ID-r.r XF-0008-0078-9 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602, 20 L 3M ID-r.r XF-0008-0077-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602, 200 L i i I