Document REdaNNQ0adnnM4wrYpev2Gwz
M S .& 6
0 < 3 0 ,7
MICROBIOS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity: Mixture containing Perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-3072. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-3072. Current information indicates it is a mixture of 1.67% test substance, 33.33% diethylene glycol butyl ether, 29.23% water, 20.83% urea, 5% sodium octyl sulfate, 3.33% sulfonated alkyl ether sulfate salts, 4.60% acrylic foamer, 1.67% triethanolamine, and 0.33% tolyl triazole.
The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the fluorochemical component
METHOD:
Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions:
Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 2000 mg/L primary stock solution was prepared by dissolving 200 mg test substance in 100 mL Millipore Milli-QTM water. A secondary stock solution of 520 mg/L was prepared by diluting 26 mL of initial stock solution with water. The pH of the stock solution was 6.8. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. All test solutions were made by proportional dilutions
Exposure vessels: 4 mL cuvettes Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
002935
RESULTS
Nominal concentrations: Blank control, 32.5, 65, 130, and 260 mg/L
Element value:
5-minute ECso = 305 (282 - 330) mg/L 15-minute ECso = 184 (177 -192) mg/L 30-minute ECso = 139 (134 -144) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS
The test substance 30-minute ECso for Photobacterium phosphoreum was determined to be 139 mg/L with a 95% confidence interval of 134 to 144 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES
This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number M3597, 1995.
OTHER
Last changed: 6/25/00.
002936
06/25/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
1
LAB REQUEST NO. M3597 REQUESTOR NAME: SBS/KEN GOEBEL DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 11/29/1994 DESC: FC-3072 AFFF BASE
CONTRACT LAB(S): ASCI EXP COMP DATE: 02/06/1995 DATE COMPLETED: 03/24/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
TESTING IS TO BE DONE IN-HOUSE.
Sample sent to contract lab for toxicity to fish evlauation due to problems
with lab fish cultures. SAB 2/20/95 Results from Microtox testing:
Test Cone. mg/L 32.5 65 130 260
Percent Light Loss as Compared to the Control
5-min exp.
15-min exp.
3 0 -min exp
10 12 15
16 21 27
27 37 47
46 62 74"
EC50
305 184 139
sab 3/16/95
Results from Fathead minnow toxicity test: After 96-hours of exposure, all test fish were still alive in 1000 mg/L test substance.
SAB 3/22/95
Activated Sludge respiration inhibition test results: After 3-hours of exposure, there was 15% inhibition in respiration at 1000mg/L
SAB 3/22/95 Results from Daphnia magna toxicity test: No mortality or immobilization was observed at 1000 mg/L after 48-hours. SAB 3/23/95
SAMPLE DATE
CODE
DESCRIPTION RESULT
MDL or (95% C.I.)
1
FC-3072
LIQUID
ASCI
48-HR-EC50 DAPHNIA ACUTE TEST 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR MICROTOX MICROTOX - EC50 SLUDG-RESP SLUDGE RESPIRATION - EC50
* = CONTRACT LAB DATA
>1000 MG/L >1000 MG/L 139 MG/L >1000 MG/L
(-) (-) (134
(-)
002937
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET
TEST SUBSTANCE:
L. *-)C-~7 Z-
LR:_ M j / r l 2 _
Date:
S'
Analyst: ( jts j f
1. Preparation of Stock Solution
* Mass/Vol. Test Substance: .ya / /_ >(
* Final D ilution Volume:
,?.Y ! :
* Diluent:
/ /"< - /w !
i l ^i. i i *
* Final Concentration of Stock Solution:
. { _c_ . 0 )
f
2. pH Adjustment of Stock Solution
* in itia l p H o f Stock S o lu tio n :___ y
*p H Adjusted to:
_____ -
*p H Adjusted with:
_____ -
3. Osmotic Adjustment of Stock Solution
* Osmotic Adjuster:
j^
1Comments: -OC'Cs-.d A 1 * U J / i . o L <
/ ---
4. Appearance of pH and Osmotically Adjusted Stock Solution
f * CLEAR or OPAQUE (circle one)
* Color: * Comments:
v::
Sr. file 1
i i-V - 1 1>*
Ceauidf ,;V
1
u /lrr^ J /y j
J7
if/iC s t-
/// ^ cVtC r f i r <>*>
o
' /u / '
5. Comments
ir,*. -f
V: Ok ICO.Zv t>, 'k .'i r . S- f"<1c]
.3. >:
S P S S 'S .
/ l ) S k c . L S c /su. ; Sl G ...CL. vjdC"e . <v!rjy.*J
Irr Tja i k i - +r. +6.j kv*
U
y (J / a;
-v
s | I f;: ;'i'.S. "fi:.
, / -o.fi;,'4",5'r^* . - 7 >
. - . *w>w,
7v`::4;
J 1t^.h-jsf.KVSfr>'
002958
BEST COPY AVAILABLE
BEST COPY AVAILABLE
4 1 *-.' 5 ? , i l V.
I^T
*>
f*L -
J (-
T *l '
'
-1 -v
--u-JiK- ' `
`* 5ij
"3 c f J Q |>
*`i *
C*'* ,, -,A%
r<voC*fyOu
STATt^nCA
^ J r 4 r ':^V.........
iTIN tQijrii lCH-'r t OB ct
^7JL^a:!0 i*
. O..W. isJk3.5TMtf*?, i>: 'f&zxC**
-2,4847 ' *- it ,v < i
^OFjipA.TA ^ l A S ' t J S g
& 'S,r$?
I k fi * * 1
v. ...... V,*>i-K-.Jtvy*..
^^APIANCLi \A j|j
^ F l t * HOT f A C TO R II
w ^ l iV-kvi'i* '-" w ..
irii
cfc* -
>
' ' ^ 1 >J*>
I eHT Ufv:'DHTEkM
'$3*. ** . *
P&FOR ECb
t t i'
-, J > .
_ .. f.--wVi-ft'lI ;-iy
mi
I "&'
M-
zv*
fciir
-.- iy 'y if tf f' s -
. yf We 'i
!?*;... '-ra fM
;r<*'1SH
002940
BEST COPY AVAILABLE
BEST COPY AVAILABLE
- ':-M
- ' v?\;
^5v'
*f 8g 3^ fs^ 'V l r: > >aV*w W
<r> a >
- ^ r VTj'- -it*
' ' -' 'I /A *
> < 4 .
-
'
* ""I i i - ' *
10'
;H2*m 35T7
V! ki , 'i f < V '* - < s''
002943
....... ........... .......
BEST COPY AVAILABLE
*J
* "7c f i o
,rf^ N U M 5 E iS i /. ': k C-ONCEN tftA Tl;>-
. ;, . ....-
...........GV j&:fWJ S ^
S i .>< ` X V&5? - 'y
'O.JWSiS* .^4 4
002944
BEST COPY AVAILALt
BEST COPY AVAILABLE
ti A
>,?** Xi.' -t 'i * ^ '
N?
i? 1
... - . . i
.*
- * '
'. ' V'*r *
- *` ' " * * ' ' * .j *'j - v V k* F"'iV
JAflOKi UK - C f".(W/i V C P B o ? , 1 4 j 4
- . (*
5%
* . ,
fflfltee t J <?*<?o'0\
- ^
tv \ i
"v >, A v >
IDFWCf- rACTJPns ^ f C A V f c
liv ij
&tdJj^i-VJ*I*!y?**yV/ *
> ** , ti* '1
^ w`:
V ?*
^ &^W--
IS *$ & **
i'iShiij r - lVo;9i
` v fcfe?
in <
feSSAU
S&:
002946
BEST COPY AVAILABLE
