Document R2ymxapBVV3pDd7YmDX4wVKRV

BACK TO MAIN 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECT OF L-13492TO MICROBICS' MICROTOX" TOXICITY ANALYZER SYSTEM REPORT COMPLETED: 7/26/95 Prepared by: 1 Susan A. Beach Adv. Environmental Biologist 3M IEnvironmental laboratory Bldg. 2-3E-09 935 IBush Ave. SI. Paul, MN 551441 BACK TO MAIN .. MlcrotoxN Inhibition Page 1 July 26, 1005 Microbks' MicrotoxTMAnalyzer is a temperature controlled photometric device which measures the reduction in bioluminescence of a naturally occurring marine bacterium, Photobecferium phosphorem, in response to a chemical toxicant The NlicrotoxN System pmvkies a simple and inexpensive method for the rapid screening of microbial toxicity of chemical substances and waste streams. It requires only a small sample she and permits duplicate determinations of one sample in 30-minutes. Toxicity of a chemical compound is reported as a percent decream in light output. Similar to conventional bioassay toxicity data (e.g.. LCso), EC50 is used for MicmtoxN data. EC50 is defined as the concentration of a toxicant causing a 50% reduction in light output firom the base-line level. lk&abmw &P=m -rn L-13492, Lot 2, 45% solids, 5/4/95 Clear, colorless liquid Aqueous Solubility Apparently completely miscible with water. Physicochemical Properties: See MSDS. This sample contains 44.9%product, 27.2?'0isopropyl alcohol and 27.9% water. Date Received Not recorded Storage In the dark at ambient room temperature (22 2%) Stock Solution Reported values Blank. Exwsura: Testdate 200 md100 mL Millipore Milli-Onl Water. No color was apparent. Initial stock solution pH = 4.7; adjusted to pH 6.7 with 0.1 N NaOH. The osmotic strength of the stock solution was adjusted to 2%NaCl by adding mg NaCl to 10 mL stock solution. values were based on n beginning of the microbi Contact time BACK TO MAIN output) derived from duplicated determinations were used to calculate the median effective concentration, 'EC~O', and the 95% confidence limits by least-squaresregression statistics for loglog data using a computer program provided by Microbics. 0 values obtainled after 5, 15, and 30-minutes exposure to the test substance L-13492 are summarized in Table 1. The 30-minute median effective con determinations and was reported as 260 m n, 'EC50' - was derived from duplicate dence ilnterval of 220 300 m@. Gary W.Jorgensen. All raw data test facility, Blclg. 2-3E,SI. I ntrol correction factors calculated 95% confidence r-. I 51 The correlation coefficient for the EC50 calculation for all three exposure periods #as > 0.9. i The coefficient of delerminatio ree exposure periods was > OB. The remrted values were base assay. lest media at thn heninning of BACK TO MAIN Microtox" Inhibition p-3 July 26, 1995 This study was not conducted under strict Good Laboratory Prectices, but was conducted under the stendard operating procedures for this laboratory. The raw data was reviewed by S A Beach prior to reporting. Copies of all raw data are included with this report. This study was conducted under 3M Environmental Lab IRequest number N2169. 1 ) Microbics Corporation. 1989. How to run toxicity tests using the MicrotoxTYModel 500. carlbbad. California. (1) Mean light loss of two replicates. i BACK TO MAIN ENVIRONMENTALLABORATORY: MICROTOX PREP SHEET p j I b f l C ' TEST SUBSTANCE: - 92 1. Preparation of Stock Solution 2.li * MarSNol. Test Substance: F h lDilution Volume: Dilrznt: fl,'/l*k/,O 1/mfLl Final Concentrationof Stock Solution: -?3 / L 2. pH Adjustment of Stock Solution InitialpH of Stock Solution: L/: ';r pH Aqusted to: 'pHAdjuted wirh: &. 6,3 J&u0L!! A& I of a , s d +Qdud 1d c o p u f O . o t ~ & ~ ~ 3. Osmotic Adjustment of Stock Solution W W I. ts: 5. Comments BACK TO MAIN -' J G .3 I; .? M? M 3 ci d BACK TO MAIN A PE STATISTICA REPORT ` BACK TO MAIN an a al :3 Erse phEd :$ sSiekrosnnss Tsiias pyeflc LRN216D Voie ifis Gs Aiw cots ae ui nine Caen a-- rae no omen men mm am EE 627.7010 anil 193% COMTITEIGE RANGES 92.2359 10 6a. 4155: BACK TO MAIN Aaar-ovad uv: -. . . '5 .3 n -5 *"' .3 if! .5 ;I .? BACK TO MAIN pqCefies LR N2I61 Be ---- conseLaTion GopBFIGIENTs R= b.991 Ty -- Sol Suh ar SSH R mana 14 ExrmssoLATED RESTS BACK TO MAIN Pg ToFIC LR Nzleq i 20000r oiars pr LEE BACK TO MAIN - 4 BACK TO MAIN 4 PIefic LR Nazi] STATISTIC. RERCRT FoR GTA FILE 210960450 STONATIN CORIO 05C eR oF Dara Fates used: = 0.9972 3 4 Las 8 oss Ast aRINCES 0.00005 Bm a (mmo EI IIELL Cumin um net BACK TO MAIN I 2 3 03/29/2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT 1BACK TO MAIN LAB REQUEST NO. N2169 REQUESTOR NAME: SAB/S.J. MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 05/17/1995 DESC: L-13492 ECOTOX TESTING CONTRACT LAB(S): EXP COMP DATE: 07/03/1995 DATE COMPLETED: 01/30 / 1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651 -778-6176 Results from Microtox Test: Test Conc. mg/L 125 250 500 1000 EC50 95% C . I . sab 5/19/95 PERCENT LIGHT LOSS 5-min exp. 15-min exp. 13 29 19 43 37 65 75 92 630 30 0 (590 - 665) (270 - 330) 30-min exp. 34 51 75 97 260 (220 - 300) Activated Sludge Respiration I n h i b i t i o n Test Results: There were some d i f f i c u l t i e s encountered i n o b t a i n i n g t e s t r e s u l t s . The i n i t i a l screening ( l i m i t ) t e s t i n d i c a t e d t o x i c i t y and t h e need f o r a study w i t h l o w e r c o n c e n t r a t i o n s . The second s t u d y showed low t o x i c i t y . The t h i r d s t u d y was i n v a l i d due t o unacceptable d i f f e r e n c e s i n c o n t r o l r e s p i r a t i o n r a t e s . The f o u r t h s t u d y a l s o i n d i c a t e d low t o x i c i t y . The f i n a l s t u d y was i n i t i a t e d t o c o n f i r m t h e second and f o u r t h study r e s u l t s . It i s p o s s i b l e t h a t i n t h e f i r s t study an e r r o r i n t e s t s o l u t i o n preparation r e s u l t e d i n l o a d i n g t h e t e s t system w i t h more t e s t substance t h a n planned. T h i s c o u l d have induced a g r e a t e r i n h i b i t i o n i n r e s p i r a t i o n r a t e . SAB 7/31/95 Test Conc. mg/L 1000 Duplicate EC50 Percent I n h i b i t i o n i n Respiration Rate 30 - min Exposure 3-Hour Exposure 0 18 0 22 >loo0 >loo0 SAB 7/31/95 NOTE: 3-HR r e s u l t s updated 2/19/96. An e r r o r was discovered i n c a l c u l a t i o n s o f percent i n h i b i t i o n . sab 2/19/96 SEE LR N2332 FOR FISH, DAPHNIA, AND ALGAE TOXICITY TEST RESULTS. SAB 7/31/95 1 05/04/95 L-13492 LIQUID MICROTOX MICROTOX - EC50 260 MG/L 03/29/2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT (220 - 300) 2 1 05/04/95 L-13492 LIQUID SLUDG-RESP SLUDGE RESPIRATION - EC50 > l o 0 0 MG/L BACK TO MAIN