Document R26Z02Vwd8KDLyLbwY7Bj96pk
BIOACCUMULATION (ANALYTICAL ONLY)
TEST SUBSTANCE
Identity: PreefrefrlrueodrotoocatsanPeFsOuSlfAoAn,ylFamCi-d1o2e8thoyr)aacsetthaetem;ajmoarycoalmspoobneent of F[(Ch-e1p2t7ad,ecFaCf-i1uo2r9o,ocFtCy-l)1s0u9l,foonrylFlC--,1p0o9t-aXs.si(uGmlyscailnt,e,CNA-Sst#hy2l9-N9-1517)
aRemmiaxrtukrse:of 8T6h%etteessttssuabmsptlaencies FaCn-d12184.%CCur.r-eCntfliunofrooramlaktyilocnairnbdoixcyaltaetseit is compounds,
METHOD:
Method: bDieovaicsceudmbuyla3tMe fClouomrpoacnheymitcoadlestferrommin3eM'ifsblDueecgialtlurs,unAfLishplant
GLP (VIN):
effluent No
(See
Remarks)
Year completed: 1978
Remarks: The following method was devised and used:
1. 2.
Two lots of 30 fish each were used. One ot was exposed to Decatur plant effluent from Oct. 5 to Oct
25. Oct.
The 3t0
other lot Oct. 25
was
exposed
to
Tennessee
River
water
only
from
3. TthweelrveemafiinsihnfgroemigthhteeeenfflfiusehntweexrpeossaecdrifloitcewderineDceoclalteucrt.edTdheeadriavenrd
water exposed were sacrificed
fish survived in Decatur
through
the
exposure
period
and
all
4. aEfnfdlusehnitp-peexdpoisneddryanidcertiovetrhwea3teMr-EenxvpiorosnemdefnitsahlweLarbeoralaltporreysienrvSte.d
Paul, MN for analysis.
5. 6.
Fish Two
were homogenized using a 2g. portions were weighed
Tekmar Model and extracted
SDT using
tissuemizer. ethylacetate
7. oSnamopnleesanwderteolauneanelyoznedthbeyoGthCe,r.TLC, and GC/MS.
RESULTS
Analytical Observations:
The fish
effluent-exposed fish weighed 33.0 grams.
weighed
25.7
grams.
The river water-exposed
conta
GC/MS did not
daentaelcytsitsheofpretehsyelncaceeotfatFeCe-x1t2r8acted
fish
exposed
to
plant
effluent
No fluorochemicals were detected in the river water-exposed fish.
mRaenmuafrakcst:uresSftluudoyrowchaesmiccoalnsd.uctWeadtebrecuasuesdei3n Mm'asnuDfeaccattuurrinpglainstlater
treated River.
and
the
treated
effluent
discharges
eventually
into
the
Tennessee
C0 CoOnNcClLuUsSIiOo0 NnSs 0000 No reliable conclusions can be derived from this study.
Submitter: 3M Company, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
oDaAmTaAQQuUAaLyIYTY 00
00 0000000
mRealniuafbaiclittuyr:inKgifmaicislcithy rraanthkeirngth3.anSattu3dMy'wsaEsnvciornodnumcetnetdalatLatbhoeratory.
aEfnfdlutehnet scpoencciefnitcrpartoitooncsoloffosrubejxepcotsfulrueorofocthheemificsahlswawserneotnoftoucndh.araTchteerriezed
ewfafslueanltsousneodiinnfotrhmeatsitoundyon(e.agn.a,lNysHiss-oNf itnheefTfleunenntesissekenoRwinvetrowcaatuesreordeath
idfutrhienrgeNwPaDsEaSnyteosptpionrgt,uonritlyowforDOd,ephuirgahtiToDnS)o.f thAeddfiitsihonparliloyr,toitsiascnriofticken.own
fPirseh sceonncceeontfratthieotnessthsoumbosgteanniczeincgouwlidthhtahveeebffeleunenat
normal function concentrations.
of the No
tehxepleafnfaltuieonnt-isexaptotseemdptgreodupa.s
tNoowhdaiftfewraenstitahteiocnawuasseogfitvhene
12 dead fish in to whether the.
subject chemical was the surface of the fish
abcotduya.llySaamcpcluimnuglaltoecdatiinontshewefirseh
or simply sorbed not described.
on
RReErFeERENCcEESS
V3MrTaecs/hnApirctiahlurrReMpeonrdtel",AnaPlroyjseicstfo9r97Fl0u6o1r2o6c0h0e,miFcaatlesoifn FBlluuoergoiclheFimsihc"alGs.,A. Report Number 14, May 1, 179
3M requested Gillett, Comell
eUxnpieverrtsiotvye,rvMiaerw,ch"B8i,o1a9c9cu3mulation
Studies",
Dr.
James
OOTTHHEERR oe Last changed: 5/18/00
r------
enosts
>
3m.
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.26
(im=proeporrtstpitaa anbtoth siodfpaepesr, twocopie 0 TCC.)
"same
Fate of Fluorochemtcals Environmental Laboratory (EE & PC) Reg. No. 48765 D. L. Bacon Analysis for Fluorochemicals in Blue, 111 Fish
0535
.
014
G. A. Vraspir/Arthur Mendel
48838-18
seeomirvh R08,comin FoiSumorieion eReOoetnSss,eimno, ConnENT GRiECTIVE:
| Retin
Status Report
7641643939
7
LrSwatrad :
fReEPOE NTABSTS RACT. (20025e 0 rcTh ony ttermaion vioned $4TetComrie
c:VARl..NPAo.VtPehrlaotpkerorap-g-2a21d3-a62-- 286-34 RIL:Bohon-21-21
enn1e
FC (Req. 4876S)/GAV/AM
-2-
5/1/19
OBJECTIVE
Tffhliseuho,rpoucrahpfeotmseiercaoelfxsp.othsiusreitnoveosutrigDaeticoanturwasplatnotdeetffelrumeinnte, ifbibolsucecguimlullated
SUMMARY
Extracts from bluegill fish exposed to Decatur plant effluent wT(eLPErC)eicnnoodtnitcdaaeittneeedcdtteh1de0. pprpemse(nucg/eg)ofofFC-F9M5,392w3hilaendF7C-1p4p3m oafndFMFC-3412228.
Eexttharnaoclt,s eftrhoymlbleutheegri,lletfhioshxyeetxhpyolsedacettoatrei,vertowlauetmeer, No fluorochemicals were detected.
(aRWn)d
xcyolnetnaei.ned
eAxpceorpiymeinstsa.ttached of CRL report 7124 which detailed GC/MS
INTRODUCTION
Tbyhefuipsthawkeereandrepcolretaerdanc(e1).of Tshpeecicfuirrcenrtadiroelpoarbteleddetafillusortohcehemicals
Daencaaltyusresplfaonrt felfufolruoecnhtemiacndalsininfisfhisehxpporseevdiouosnllyy etxopToesnendestsoeeour
tRoigvrearphwya,terand(2)g.as used.
Tchhirno-mlaatyoegrrapchhyr/ommaastsogrsappehcytro(sTcLCo)p,y
(gGaCs/McSh)rowmear-e
DISCUSSION
2
*
mOeauvnreuntfDuaeacclatltuyurrinignptloainstthlemaatneTurefnantcertseusareteeesdRiafvnleduro.rtohechBeltmurieecgaaitllesld. effisWfhal,uteenrcthousdseeindschfianorrges : tfihsehsewebrieoasesxapyoseedxpeornilmyenttos,thweerTeenneexspsoeseedRitvoerP.Ew.aterAno(t2)h,erThleot of iftnihsrhethewe-erreoexupteetrhiepmnreoncatenadalulyrzeseedcatsifooonru.tlfilnBuerodireofcilnhye,mFilcofawilsshDibwayegrreaanmho1imnoavgnoedlnvidezedetda,iled wa(hnRidoluetseomseo2)m.eofoftAheptohrehtohimooomngoegnoeafnteattheewaswlaasetxtteerrxatcretaxectdtreacdwtitwhwiattsholceutoehnnycslen(tRarocauettteeadte1), Rseocuttieon3.) for GC/MS and TLC. Results are given in the summary
cna?
an
FLOW DIAGRAM 1
ANALYSIS OF BLUEGILL FISH
.
(13)0 eFiisghn
*
(2) "Tissuemize"
p>
0,
(1) Weigh 2 g.
(1) Weigh 2 g.
(2) Extract (ETOAc)
(2) Acidity
(3) Centrifuge
(3) Extract (Toluene)
Layer (4) Isolate ETOAc
--
oc FFHor3923
FM 3925 FM 3422
Route 3
Concentrate hy
GC/MS
TLC
For
. (4) Centrifuge
(5) Isolate
Toluene Layer
(6) Methylate
Ll
"
ac/us
5
Rie
(Ester) i
FCFCC1--1294583
oeeass
"FC (Req. 48768)/GAV/AM
-a-
5/1/79
EXPERIMENTAL
Tbtwyiovetlhlyeo,tEsnavnoidfrostnhhmiieprnptteyadlfiinLshabdoerraycahti,ocre,yw.raweprpTeehdereifcnisehiavlweuedmrieonnukmNepoftvoielmf,rboezrreen3's,puenc1t0-7i8l,
Oacnta.lyz25e)d eighteen
wf(eNioisvhg.hweed3,re251.s07a78c)rg..ifiTcTwehedelveienffDlfeuicseahnttuw-ree.rxepoTscheoedllerlciottveerd(-Oecdtxe.paods5eadntdolot
a(tOctD.eca3tutro. Oct. 25) weighed 33.0 grams and were all sacrificed
wTaos siamnpalliyfzye,d thine were used:
eexxapcetrliymentthealsaommee
mlaontneirs. diTschuessfeodl.lowTihneg
cootdheesr
River River
Water Water
Holding Holding
Tank Tank
Exposed Exposed
Fish Fish
-
eextthryalcatcet(aRt.eW.-EtAc) toluene extract
PPllaanntt EEfffflluueenntt S5--11 EExxppoosseedd FFiisshh -- etotlhuyelnaec(eRte.axtt.er-aTcoetlx.t)r(aPc.tE.-(TPo.lE..)-EtAc)
TtTWuhbOees2f.igs.h`Tpwwoeorrtemilo'hsnosmoofwgeernpeiezsetwdieciigudhseeidnggrianadteoTekesmteahpryalraaMctoeedtealptoelSyDewTtehrteyilsesanudeedmeidczeentrot.rifuge
VotnooertteahxnedoGte2hneimrle. mcioxnEecar.c.hHCslTahmepplleussamwpa2slemslmixwoeefrdeAaRpcpegrnroatxdreiimfauttgoeellduyenfe1orwmie1nr5.emioannd.deadat
eo1fx0ft,r0a0ac0ntdrwppalms'asc.eedxa"giTinhneegdleatsuhssyilnavcgiaelttsah,teerfeaosnlpdleocwttioinlvgueelmGyeC. ecxotTnrdhiaetciteostnhswy:elraceeItdnasrttarew.n -
H1P0%5C71a3rbowwigtxh 2Ni0 Temp - 200 C.,
Defoe.nle6Tce0tm/rp8o0nm- ce3as0p0htuCrheCr.o,dmeoOtsveocertnobrT.eW-mApWC.o-lu15mI0nng.- CP6o'irxstot1h/-e8r"ma5l.,5.
iL
Flow - 40 cc/min. Argon/Methane (95/5).
TuALnfCdteerrconNGjfCiipatuniraoglneyssiwfseorreoftuwsoEetdAd.cimeeTxnthsrieaocnctao,lncTeaLntCproarattneiadloynsEewfsaA.sc
cTohnecenftorlaltoewding extraces were
sMineproctEktteAdcsilaainldcoandgrsgieieldde.plsattTaehnsed.nardtshTeheoyfplwaFetC-re0es5,wfeirrFesCt-1t4du3re,nveedalnod90peFC-ain1nd281d0%oenvEeEtl.OoHped =
wiinth500%.0E1t%OHNeiwn FEtuAcch.sinThine wdartieerd p(l3a).tes were visualized by spraying
MeTiPhx" eggitneoelldeucetnuresoinnegxtcrataphcetturfewoalsdleotmweeictntghoyrlG.aCtecdCoonldwiuitmtinhond-si:1a2z'oxmInest1th/ra8."neHS.PaSn,d571t23h0e%wnith
42D00C0- 3c0o0/C.miann.dDet1A.0r%gotneB/meMpnetto-hnae30n0e3 4 (9oC.5r,/58)O0.v/e9n0Thm-ees11he0thgynlaCakcrIeostomatthPAee.rmaanldi,njm.eFtlPhooywrlta~te-d
GtCo/lMuSeneanealxytsriascts(Rewqe.reNos.ubmCi-5t0t6e8d7)t.o for GC/MS conditions.
CSeenetraalttaRcehseedarcChR
RAenpaolryttiNcoa.l
7f1o2r4
oen3t9
. "FC (Req. 48768)/GAV/AM
-5-
5/1/79
PREW/E&tAPcE-Teoxlt.racsthowsehdowendo daipfpfreorxeinmcaet.ely'N1o0FCu-g1/4g3oofrFFUC-3142228.was Rdyetewacstedthabty TofLCroeffertehnecePEFECt-A9c5,. but a spot was noticed whose
REFERENCES
(1) a) BM.luTe.gilEllnaSbuanrfaiwsyh, an"dBioInconCchaennnterlatiCoantfiosfh,F"M E3E42&2 BiCn
Report, 5/17/77.
.
b) FAi.l N3.42W2e,l"teErE, &"PACquaRteipcortF,ate10/o1f4/A77F.luorochemical,
(2) ODcetc.atu18r, Ef1f9l78uenatndFiFsebh.To5,xic1i97t9y; TeMs.tT.ProEtloncaoblasr,awyd.ated
(3) 2J.3, Sl1i21wio(k107a8n)d. A. Macioszezyk, Microchenica) Journal,
B84 Vooaps spo
ct7,s
GAV/AM/cen Attachment
.
>
v:
3
.
000310
ro
CENTRAL ANALYTICAL LABORATORY
Report No... 2M24 ____________
Date. March 28, 1979
Subject: Decatur Fish Bloassy.
Requestor G:_ Vraseir, A: Mendel
ReNq o. u S8e 87 st
Report: INTRODUCTION
Dept. Name EESPC
Daea Nove1m6,1b97e8 r
Proj. No.915016130404
TrDSeueanncmnnapietlnsuegsrsewePIelnatrRnoeitv.etrrheecwFehriiiisvvcheehdrw.affrlrsoowEnstehxtyJphluoessteardceeoqtuutateotsseittdoweeroxstdtihfferfopemlraaannttfiwnsdah.tebrithoess'sysapybreocmtssarksetnhreaitvetnhe duisaiznogmetthheanGeC/MaSndsyasntaelmy.zed.Toluane extracts orafcttsheoffisthhewefrIeehmweatrhey.lescnetdlvcernosn Disucssion
#JRT2ahrMeweeSrya4e2mnpaallaneynasdzlewydPzeLEeru.desinu1lgs2aibnce(golenetddchyioltnAi.dVoi'.antcsiaotn1fasotreanfdceorxotPlcr.uaoEmc.ltusA1).be(lmoSewetmhpaylnledastseaRds.ptioelsFu1sNnaeun.eext#br3eaecatsns)) Pacn.g
mn 8 salon
OCIIvonneljsneut:cmrtnuomreA1::n1t0:C1126!5VIasCSro.itSha.enrm22a07l840DfoCgr-a2s0101chm+rionm1ua0tt3eosgBraatnphtheonre 13140-o1n70ACnakeerom10oPeA/min.
.
OCIvoneljneu:cmtnorB5::0 -26'222055.5C.atCW1200HC/m1i5n%.on Chromosorb W
:
CDaertreciteorr:Gas:VarHieanliufmlaamte 2I5oncioz/amtiino.n at 230C .
MSIoondusert:creu:mealnltC:hesmaimdcpualPleosntlwoen2r1ze-a4tr9iu1on8nmienssElescptercotnromleotneirzatlon, ras als run in Chenlcal Ionization
sample
PE.
#2
MHMauesllstiiupnRlainSegerep::aratB1oe8rn:d-ix1SC7ihnamgjnlene.elsttraomn ge with Ny gar
ease
7 Revert No. 12k
Pl
rn <u, a
RESULTS
eSaxmtprlaecstinRgW.end#1 meatnhdylP.atiEn.g:JI etshhaonwoeld, byethGyCl/NSetohnerl,y
the solvents ethoxy ethyl
used in acetate
toluene and xylenes.
oIbttawaisn sneucfefsiscaireynttdoatalartcoonicnetnetrrparteet tthhee metahsyslspseccettraat.e extracts In order to
GT.hCe.TepnenakessseneoneRiovferwheixcphoseadppefairsehdatthoylconatcaeitnatefleuxotrirnaec.t cTohnetacinoemdponnuemnstrsous pahptpheaalratteo, coanrdreshpiognhdmotloecruelfaerranacleksenoesf f2-rmoamtahbyolu-3t-oCcytsantooneo,t alceeatsitcCoayc.id, a
wThhiechplaanptpeaefrfltuoenctorreexsppoosnedd ftioshreeftehryelnceascetoafte2-emaxtthryalc-t3-coocnttaanionnee,d comdgphotiucnds "58i2d,n/eF,luonriotcrhoegmeincaclosntwahionsienghifglhueosrtocmeahsmsiceflrsagm(ewnhtossesheiagnhewsatremasMs3fWraegmeanntds
Seen were bi8 m/e and 584 m/e), FH 3923 (FOSE amide) and FM 3422 (FOSE alcohol).
SURNARY
RThiivserfwisahtebriocaosnstyainsehdowesduchthcatompeoxutnrdasctsasfertohmantohle, feitshhylexpeotsheerd, teothtohxeyTeetnhnyelsses apcleatnattee,ffltuoelnuteneconatndainxeydlenFeO.SE aTmhiedee,xtraalcctohsolf,roonthtehre frieslhateexdpofsleudortoochetmhiecaDlesc,atur 2-methyl-3-octanone, and scetic acid.
PR Jeong
"I. N. schroepfer ren
.
?
.
00a3E2R