Document R21Va5x8we97x75x01kKxVOpE
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Study T i t l o
A C U t O TOXiCfty O f N2803-4 to t h o Daphnid, D8phnf. mogna
Data ReqUfr.P.nt: Oui.d.lin~ Umber U.8. EPA-TSCA, Guideline 797.1300
Authors
T h t h y J . ward Peter L. Kowalski Robert L. Btmri
Study CQPp1at.d
November 28, 1995
Roport Anended
w u c h 25, 1996
Sponsor
3H company Environmental Laboratory
935 Bush Avonue Building 2-3E-09 S t . Paul, Minnesota 55144
Testing rncflity
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j ' T.R. nilbury Laboratorier, Inc.
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8 40 Do- Lime
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Xarblehead, Warsachuretts 01945
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I T.R. nilbury study uurnb.r 895-TIl
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APundod 3/19/96
This study was conducted according to EPA Good Laboratory Practice Regulations (40 CIR 792), with the follwfng exception. The stability of the test substance was assumed but not vmrified at T.R. Wilbury Laboratories. Deviations from the study protocol and protocol anwndnunts
are presented in section XI1 of thiii report.
The final report wan amended c m m c h 25, 1996 to include the date of the iumndnunt on pager 1 and 3, to allow for signatures to the Amended report on pages 2, 3, and 18, to modify the 48 hour ECSO on pages 6, 13, and 16, to modify the definition of the EC50 on page 12, and to include the amendment summary on this page.
study Director
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, T.R. Wilbury study Number 895-Tn
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Submitted by:
T.R. Wilbury Laboratories, Inc. 40 Doak8 Lano narblohead, XaOsaChUaOttS
Certification:
T.R. Wilbury Laboralroriom, Inc. study nuaiber 895-T8, & U t e T o x i c i t y o f N2803-4 t o t h e Dsphnid, Ddphnfd magna, was a u d i t e d by t h e Q u r l i t y Amsuranc:e Unit f o r compliance with t h e protocol, aitandard opera.ting procodures, and AppliCablO m o d Labo.catory PrsCtiCe. (U.S. CPA, 1993). Q u a l i t y assurance a u d i t s were performed and t h e f i n d i n g s r e p o r t e d t o T.R. Wilbury Laboratori.em management and t h e study director on tho following drtteet
Aspect Audited
Protocol t In-life:
Draft Report: F i n a l -port: Amended Report:
Audit
-- D t r t e
8/04/95 9/(I 8/9 5 9/28/95 11/ 0 3/9 5 ll/i8/95 3/25/96
Reported t o E:tudy
-- D i r e c t o r
8/014 /9 5 9/08/95 9/28/95 11/03/95 11/28/95 3/25/96
Reported to
Hanagament
8/04/95 11/28/95 11/28/95 11/28/95 11/28/95 3/25/96
n
quality hsurance officer
T.O. Wilbury Study N u m b e r 894-Til
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SECTION:
I. Cood Laboratory Practfco Compliance Statement
IT. Quality hsuranca statement
111. T a b l e of Contents
IV. Index of Tables and Figurea
V. sununary
VI. Genera]. Information
VII. Introduction
VIII. Hethodm and Xatsrial.
IX. Results
X.
Reference.
XI. signature Page
XII. Protocol Amendment. and Deviationa
Appendix A. Water Quality Data from Toxicity Test
PACK
2 3 4 5 6 7
8 8
13 17 18 19
20
T.R. Y i l b u r y study #umber 895-Til
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T a b l e 1. Table 2. Table 3. Table 4. Table A.1. F i g u r e 1.
Chemical c h a r a c t e r i z a t i o n of a r e p r e s e n t a t i v e sample of d i l u t i o n v a t o r used for t h e t o x i c i t y
t e s t v i t h daphnids, Daphnia magna, and 'U2803-4
T e s t system sunnnary for t h e t o x i c i t y t e s t w i t h N2803-4 and daphnids, Daphnia magna
s u r v i v a l and sublethal e f f e c t data from t h e t o x i c i t y t e s t v i t h daphnidls, Daphnia nmgna. and ~2 803-4
Median l e t h a l c o n c e n t r a t i o n s (Lc50s) and median e f f e c t i v e c o n c a n t r a t i o n s ( ~ ~ 5 0 sfr)om t h e t o x i c i t y t e s t with dsphnids, Daphnia iuagna, and 1U2803-4
Dissolved oxygen concentration. c o n d u c t i v i t y , pli. and temperature measured during t h e tox:lcity test v i t h daphnids, Daphnia magna, and N2803-4
Survival and sublethal e f f o c t s of daphniids, Daphnia magna, exposed t o ~2803-4 for 40 hours
PAOI 9
11 14 16 21 15
T.R. Wilbuty Study Number 895-TH
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Tho acuto toxicity of N2803-4 to tho daphnid, DaphJIia magna, is doscrilmd in this final report. The test was conducted for 3N Company for 40 hours from September 26 to 28, 1995, at T.R. Wflbu~ryLaboratories, Inc. in Uarblohoad, MaSsbchUEettS. It vas conducted by Jeanne Magazu, Potor Xowalski, Jbcqueline Nevius, Keven Stavens, Petor IYevius, R o b e Dmri,
and Tinothy Ward according to the protocol developed for T.R. Wilbury
Study Nurnb.r 895-TH. N2803-4 bas supplied by tho sponsor.
The test was performed under static conditions with five
concentrations of teat substance and b dilution water control at a
temperature of 19.1-20.6.C.
The dilution water was filtered deionized
wator collected at Marblehead, Hassachusatts and adjusted to a hardness of
164 mg/L as Caco,. Aeration vas not required to maintain Efssolved oxygen
concentrations above an acceptable level.
Nominal concentrations of N2303-4 were 0 (control), 130, 220, 360,
600, and 1,000 mg/L. Daphnids used in the test were less than 24 horrs
old at the start of the test. They wero produced at T.R. Wilbury Laboratories and acclimated under test. conditions for their entire life (cultures that produced test specimens wore maintained attest conditions for more thbn 7 days). After 48 houru of oxpoauro the control daphnids had an avorage wet weight (blotted) of 0.27 mg. All daphnids were in good condition at the beginning of the study.
Exposure of daphnids to the test ~ubstancoresulted in 1009 survival at 24 hours and no surviving daphnidu were h b i l i z e d . The 48 hour median lothal concentration (LCSO) and median effective concentration (ECSO) are 720 mg/L ~2803-4 with a 95% confidence interval of 600 to 1,000 mg/L (no surviving daphnids were immobilizecl). The 48 hour no obaerved effect concentration (NOEC) is 360 mg/L.
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Material Teated: Sponaor:
Teat Substance Shipped From:
Reported Purity:
Study Initiation Dater E x p e r h n t a l start Date: Experimental completion Dater Study Completion Date: Archive Location for the Biological Raw Data and a Copy of the Final Report r
N2003-4 3H Company Environmental Laboratory 935 Bush Avenue Building 2-31%-09 St. Paul, Minnesota 55144 3U company 3n Center St. Paul, Hinneaota 55144-1000 loa$
August 1 6 , 1995 (prot:ocol aigned) September 26, 1995
September 28, 1995 November 28, 1995
T.R. Wilbury Laboratories, Inc. Marblehead, Haaaachuaetta
T.R. wilbury study Uumber 895-TH
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This study was sponsored by 3X Coapany, Bt Paul, ninnesota. The objective of the study was to determine the 24 and 48 hour median lethal concentrations (LCSO~)and median effective concontratlons (ZC50s) of the test substance to tho daphnid, Daphnia magna, a freshwater invertobrate, under static conditions. The test protocol was based on U.S. EPA mmthods (U.8. EPA, 1993). The report contains roctions that doscribe the ruthods and materials employed in the study, and results of the investigation. The report also contains an appendix that presents water quality data collected during the test.
VIII. )aTBODS NiD Kk!ZRIALS
TEST SuBSTAmcE:
Tho sample of N2803-4 (T.R. WiLbury Laboratories Sample Number 522) was delivered to T.R. nilbury Laboratories on August 14, 1995 in a cardboard box at ambient temperature. It was contained in an approximately 50 ml glass jar. The label attached to the bottle included the following information: *N2803-4".
N2803-4 (a white powder) WAS supplied by 3X Company, St Paul, Wnnesota. Prior to use the test substance was stored .inthe dark at r o o m temperature. The test substance wa8 assumed to have a purity of 100% active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor.
DILUl'ION WATER:
Water used for acclimation of test organisms and for a11 toxicity testing was deionized water collected at T.R. wilbruy Laboratories in Uarblehead, Uassachusetts. Water was adjusted to a hardness of 160160 mg/L as CaCo, and stored in 500-gallon polyethylene tanks where it was aerated and continuously passed through a particle filter, ultraviolet sterilizer, and activated carbon. Results of chemrcal ardysis of a representative sample of water are presented in Table 1. Water used to initiate the final definitive toxicity test had a hardness of 164 mg/L and an alkalinity of 106 mg/L as Caco,. This water containod lees than 1 mg/L total organic carbon and less than 0.1 mg/L total residual chlorine.
ZEST OXGAMIBX:
Juvenile daphnids, Daphnia magna, employed as tasst organisms were less than 24 hours old at the start of the test. They were from a single source and w r e identified using an appropriate taxonomic key. Test # p . c h n s were produced by adult daphnids that were maintained under test conditions at the T.R. wilbury facility for mre than 7 days.
T.R. Wilbury study Number 895-Tl
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T a l e 1.
Chemical c h a r a c t a r i s a t i o n of A r e p m r a n t a t i v a ruiple of dilution vater ured for tho toxicity tost with daphnidr, Daphnia m 9 n 4 .nd W2003-4.
Puawterl
U n i t of
Moasuromont
Motah Aluminum Araenic Boron Cadmium Chromium Cobalt Copper Iron Lead Uercury Nickel Silver Zinc
Nitrate Chloride rluoride Total Organic carbon Total Phosphorus
Organochlorine Peaticides
Toxaphene
Organophosphorus pesticides
Dhthoate TEPP Xonocrotophos
PCBS
Detection Limit
0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02
0.05 1 0.1
1 0.03
0.5 2
0.5 2.0 2.0 2.0
0.5
JWarured Value
UD' UD UD UD UD ND WD 0.03 ND
m
ND
ND
m
M)
UD
UD UD
UD UD
ND
ND ND
UD
ND
Uotost
1. n e t a l a , nitrate, chloride, f l u o r i d e , t o t a l o r g a n i c carbon,
total phosphorus, organochlorine pesticides, organophoaphorue
p e s t i c i d e s , and PCBs were measured i n deionized water (used t o
formulate d i l u t i o n water) t h a t was colliscted during August,
2.
-1995 and analyzed by Pace, 1 n c . t a s pact of r o u t i n e
quality terting.
I D not d a t e c t e d a t or above t h e detection limit.
water
T.R. Wilbury Study N u m b e r 895-TH
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The original culture waa obtained on June 1, 1994 from &patio Rosearch Organiamr, Haupton, New nunpahire. Jbaauruwnta made during tho 7 day. before tho s t u t of the test i n d i o a k d that the culture temperature
ranged frolr 20.1 to 2 1 . 0 . ~ and the diaaolwd oxygen concentration waa alWaya at h A * t 8.6 mg/L. The culture pr0dUC.d YOVng prior to d.9 12 a d a aubaunple of adult. produced at leaat 3 young/tdaphaid/dayfor 1 day. before the start of the test (reproductiondata for tho 24 hour. prior to the s t u t of the test cannot bo verified).
During acclimation daphnids were not treated for disease, they ware free of apparent aickness, injuriea, and abnormalities at the beginning of the test and there was no mLrtality during the 48 hours preceding the E t u t O f the test. The culture Was supplied with 8 yeast/trout ChoW d X (Lot# DCO2) and the freshwater alga, BehnamtrtuP csprimrnutum, daily during acclimation. Daphnida were not fed during tbe test. control daphnids, blotted and weighed at the conclusion of the toxicity test, averaged 0.27 mg and the loading rate during the definitive toxicity test waa approximately 10.8 mg/L.
TOXICITY TEBTIPOI
A static screening test was conducted from August 23 to 26, 1995 vith five concentrations of test subatance and h dilution water control. Nominal concentrationa of N28C3-4 vere 0.10, 1.0, 10, 100, and 1.000 mg/L. After 48 hours of exposure there vaa 1001 SUrViVhl in the control and at 0.10, 1.0, 10, and 100 mg/L and 0% SUrViVhl at 1,000 mg/L.
A definitive test was conducted from September 6 to 8 , 1995. It was conducted with 5 nominal concentrations of NZ803-4: 130, 220, 360, 600, and 1,000 mg/L. After 48 hours there was 1001 survival at 130, 220, and 360 mg/L, 201 Burviva1 at 600 mg/L (A11 .Wiving daphaids exposed to 600 mg/L were immobilized at 48 hours) and 0 1 survival at 1,000 mg/L. The teat vas considered invalid and repeated because the production of at least 3 young/female/day by adults in the culture used to initiate the toxicity teat could not be verified from the raw data.
The final definitive toxicity test was performed from September 26 to 28, 1995, according to T.R. wilbury Protocol 895-TH (Acute Toxicity of N2803-4 To The Daphnid, Daphnia magna). The protocol, which vas aigned by the study director on August 16, 1995, is based on procedures of the U.S. Environmental Protection Agency (1993). A sUBPI(IIY of the test system is
prorented in Table 2.
The teat was conducted at 20 f l 0 C with five concentrations of test aubstrrace and a dilution water control. N d n h l concentrations of the test aubatance were: 0 mgiL (control), 130, 220, 3601, 600, and 1,000 mg/L. A 1,000 mg/L atock solution WAY formulated by combining 2 . 0 g of test SubStAnCO and dilution water in a C l a s s A volumetric flask and adjuating the final atock solution v o l u w to 2,000 m l . The atock solution was mixed and then appropriate amounts were added to dilution water in test vesaela.
T.R. Wilbuq Study uumber 895-Ttl
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End Points: T.R. wilbury study number 8 9 5 - ~ 8
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Alwndod 3/25/96
Tvonty daphnidm voro indimcrfplinatoly and oqually distributod among tu0 roplicatom of oach troatmont. Tho tost vam prformod in 300 m l g h m m bodcorm that containod 250 m l of tort molution (wator dopth was a p p r o a k l y 9 cm). Tomt vommolm W ~ O randomly u r m g o d during tho 48 hour tomt (a random numborm tablo warn usod to melect tho location of oach vomol) and thoy voro loo.oly covored.
A 16 hour light and 8 hour dark photoperirod vith a 15 minuto tranmition periodvam automaticallymaintainedwith cool-vhite fluoremcent lightm that provided a light intensity of 110 footcandles. Aoration warn not roquired to maintain dimsolved oxygen concentratlons above acceptable level..
Tho number of surviving organisms and the Occurrence of mublethal offoctm (ismobilization, loas of equilibrium, loss of reflox, excitability, dimcoloration, or change in behavior) w r e determined visually and recorded initially and after 24 and 48 hours. Dead test organimmm vero removed vhen first observed. Dissolved oxygen ( Y S I nodel 18 metor; instrument number 1), pH (Beckman model pBI 12 meters instrument numbor 124), conductivity (cole Panner meter, instruaent number 94), and tomporaturo (Beckman model PHI 12 meter; instrument number 124, probe PEP4 ) ~ r maomured and recorded daily in each test chamber that contained 1ivo anfmalm. The temperature in a beaker of water incubated among the tom+ vomolm warn rocorded continuously during the test.
FEITI8TIcU. WSTEODS:
Rosultm of the toxicity test were interpreted by standard mtatimtical techniques. computer method6 (stephan, 1983) vere used to calculato LC50m and EC50r. All calculations were performed by using nominal concentrationm of test substance and the number of live or irmnobilized daphnidm. The no observed effect concentration is the highest concentration of test substance that did not cause any toxicant-related mortality or s d h t h a l effects.
T.R. wilbury study Nlllabsr 895-TII
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No in8oluble material Wa8 noted during t h l b test. Biological and water quality data generated by the acute toxfcit:y test are pre8ented in Table 3 and Appondix A, rerpectively. The meamured concentration8 of disrolved oxygen ranged from 1.9 to 0.7 mg/L, the temperature ranged from 19.1 to 20.6*C, conductivity ranged from 540 to 120 pmhodcr, and pn ranged from 8.0 to 8.3. Ona hundred percent survival occurred in the control exposure and these daphnids did not exhibit any rublethal effect..
The 24 and 48 hour LC5Os and ECSO8 for daphnida expo8ed to ~2803-4 are presented in Table 4. The 48 hour LC50 and EC50 for daphnid8 expored to N2803-4 are 1 2 0 mq/L ( 9 % confidence interval. = 600 to 1.000 mg/L). The 40 hour no observed effect concentration (NOEC) i s 360 mq/L.
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Tab10 3.
Survival and rublothal offoct data from tho toxicity toat vith daphnidr, Daphnia magna, and N2803-4.
Nominal Concontration
Of Y2803-4
Rep.
Numbor A U V O
0
24
48
hr
hr
hr
Uumbor nffeatod
0
24
48
hr
hr
0 (control)
1
10
10
10
2
10
10
10
130
1
10
10
10
2
10
10
10
220
1
10
10
10
2
10
10
10
360
1
10
10
10
2
10
10
10
600
1
10
10
8
2
10
10
9
1,000
1
10
10
0
2
10
10
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
8
0
0
9
-- 0
0
lo lo
--
Note: Affected daphnids yore immobilized at 24 houro and less active than all others at 48 hours.
T.R. Wflbury study Number 895-TI1
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F 2108 -- - -\\
n 160
r' 148
0 12-
4 0 10-
c
::8 -
$
2 --
"
130
\ \
\, \
\
\ \
\ \
220
360
6 00
Concentration of N2803-4 (mg/L)
loo0
--C Live Dopbddr
I- UMffected Dophdd_s J
~.
Jlgure 1.
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survival and sublethal effects
of
daphnids,
Ddphnfii
magna,
exposed to n2803-4 for 48 hours.
T.R. Wilbury Study uumber 895-Tti
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Anwndod 3/23/96
Tablo 4.
Modian l e t h a l concoatrationm (LCSOm) and m o d l a offectivo concontratlonm (ECSOI) from tho t o x i c i t y tomt w i t h daphnids, Daphni4 magna, and 12003-4.
Erpomuro period
tcso
24 hours
40 hours
ECS 0 24 hours
LC50/EC50
95 prcont c o n iidenco limit
Calculation method
>1,000 mg/L
--
120 Q ~ / L
600 to 1,000 mg/L
--
Binomial
700 mg/L
600 to 1,000 mg/L
Bindal
40 hours
120 mg/L
600 to 1,000 mg/L
Binomial
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.~. . . .. . .. -.... ~. ..~ .
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?irhem, Macroinvertebrate., Book of Standard..
and Amphibian..
E-729-80.
Annual
Stophan, C.E. 1983. Computer Program for the calculation of LC50 Value.. U.S. EPA. Duluth, nN. Personal Communication.
U.8. EPA. 1993. Toxic Substances Control Act Test Guidelines; rinal Rules. Section 797.1300.
U.S. LPA.. 1993. 40 CFR Part 792. Toxic substances Control Act (TsCA); Cood Laboratory Practice Standards. Code of redoral Regulations, Title 40, July 1, 1993.
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T.R. Wilbury study Number 895-TX
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Study Director Aquatic Toxicologist
Biologist
Biologiet
&.h LLr'
F O ~ : Peter C. Nevius
Biologist
33$5L
Coauthor
4A tbor
T.R. Wilbury Study Number 095-TIi
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The protocol was amended to specify the noainal concentration. for the definitive test. PROTOCOL DRTIATIOIIS: The culture produced young prior to day 12 and a subsample of adults produced at least 3 young/daphnid/day for 7 days before the atart of the test, however, reproduction data for tbu 24 hours prior to the start of the test cannot be verified. This deviation did not affect the outcome of the test and no other deviations were made from the protocol.
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T a t wilbury study Number 8 9 5 4 8
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Appendix A: WATER Q U L I T X DATA ?Ro( TO1ICI:TX Page 20 of 22
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Tab10 A.1.
Dirrolved oxygen concentration, conductivity, ptl, and tompor&ture nussured during tho toxicity tent with d.phnid8, t h p h n i ~magna, and U2803-4.
Ucdnal Concentration
Of U2803-4 (WL)
Dirrolved oxygen (mg/L) - Conductivfty (@108/cm)
0
24
48
0
24
48
Rep.
hr
hr
hr
hrbrbr
0 (control)
1
8.7
,.i 8.4
2
0.7
8.1
8.4
130
1
8.7
8.7
8.4
2
8.7
8.7
0.4
220
1
8.7
8.7
8.4
2
8.7
8.7
0.4
360
1
0.7
0.7
8.1
2
8.7
8.7
8.0
600
1
8.7
8.7
8.2
2
8.7
8.7
7.9
1,000
1
8.7
8.7
0.1
2
8.7
8.7
8.3
540 560 550 540 560 560
560 560 570 560 560 580
580 580 590 580 580 590
600 600 610 600 600 610
650 640 650 650 640 660
720 710 720 120 710 720
T.R. W f l b u r y study t4umb.r 895-TtI
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Tabla A.1. Continued.
Nominal Concentration
Of N2803-4
LWL)
Rep.
PB
0
24
48
hr
hr
hr
0 (control)
1
2
130
1
2
220
1
2
360
1
2
600
1
2
1,000
1
2
8.3
8.0
8.0
8.3
8.0
8.1
8.3
8.0
8.1
8.3
8.0
8.2
8.3
8.0
8.2
8.3 8.0 8.2
8.3
8.0
8.2
8.3
9.0
8.2
8.2
8.0
8.2
8.2
8.0
8.2
8.1
8.0
8.2
8.1
8.0
8.2
Temporatura (*c)
0
24
48
hrhrhr
19.1 20.4 20.1 19.1 20.5 20.0
19.2 20.4 20.0 19.3 20.4 20.0
19.2 20.4 20.0 19.2 20.4 20.1
19.3 20.5 20.1 19.2 20.6 20.0
19.4 20.5 19.9 19.3 20.5 20.0
19.5 20.4 20.0 19.4 20.5 20.0
T.R. Wilbury study Number 895-TB
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Y .I u 1 I & V U 1
LIl v r r L I 1I .L L c \ m I VI1 I
ARCHIVED FINAL REPORT
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LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01/1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK
CONTRACT LABCS): TRWIL EXP COMP DATE: 10/04/1995 DATE COMPLETED: 03/27/1996 PROJECT LEAD :: PHONE NO:
3M FAX NO: 651-778-6176
Samples received from S.J. Martin.
Samples 1 - 5 sent t o TRWIL on 8/11/95. 8/22/95.
An a d d i t i o n a l 4L of sample 5 sent on
Sample 1 = T e t r a b u t y l ammonium hydroxide, 40% i n water. Sample 2 = L-13492 (45% s o l i d s , 27% water, 27% IPA) Sample 3 = FC-26, 100% s o l i d s . Dissolve i n 50:50 water:IPA sol.ution. Sample 4 = FC-143, 100% s o l i d s . Sample 5 = L-13856, 15% s o l i d s . Sample 6 = L-13856, d i l u t e d by a f a c t o r o f 15, t e s t e d as an e f f l u e n t .
Sample #1 (Tetrabutylammonium hydroxide) - a new b o t t l e was ordered from
A l d r i c h because TR Wilbury needed more sample. This was l o t 05922BN, r e d l a b e l , c a t . ## 17,878,O. sab 9/11/95
NOTE: A l l data f o r sample 3 (FC-26) i s reported i n terms o f FC-26, and n o t as a 50/50 mixture w i t h isopropanol. sab 11/21/95
NOTE: For sample #5, o n l y a r a n g e - f i n d i n g t e s t was run f o r t o x i c i t y t o algae. The requestor canceled t h e request f o r t e s t i n g so no d e f i n i t i v e t e s t was run. The r e s u l t s f o r t h e range f i n d e r are as f o l l o w s :
Test Conc.
mg/l 0.1
1.0
10 100 1000 EC50 (95% C . I . ) sab 2/16/96
Percent
Difference
i n Avg. S p e c i f i c Growth Rate
96-hrs Expsure
2
18
4
32
- 100
160 (100
1000) mg/L.
For N2803-6, t h e t e s t i n g was canceled before a d e f i n i t i v e study w i t h f i s h c o u l d be completed. Results r e f l e c t t e s t i n g w i t h a s i n g l e c o n c e n t r a t i o n o f 13% v / v , which showed no m o r t a l i t y a f t e r 96-hours. sab 3/14/96
1 08/11/95 TB NH40H
L o t 10317KQ
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
27 MG/L
(23 - 32)
04/01 /2001
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
BACK TO MAIN 2
1 0 8 / 1 1 / 9 5 TB NH40H
Lot 10317KQ
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
720 MG/L 3.7 MG/L
- ( 6 0 0 - (1.8
1000) 7.7)
2
L- 13492
Lot 2
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
- TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR
TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0
62 MG/L 960 MG/L 8.4 MG/L
(36- 100)
- (830->1000)
(5.9 14)
3
FC-26
L o t 135
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
- TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC5O-ALGAE *ALGAE ASSAY I C 5 0
360 MG/L
140 MG/L
90 MG/L
- ( 3 0 0 - 500)
(110 180)
(63 - 130)
4
FC-143
L o t 427
TRHIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
5 08/16/95 L-13856
As -is
720 MG/L 740 MG/L >lo00 MG/L
- (600 1000)
(660 - 830) (-1
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
- TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0
190 MG/L
(I30 -600)
>lo00 MG/L
(-1
SEE COMMENT ABOVE
6
L - 1 3856
1 :15 diluted
- TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR
TRWIL ICSO-ALGAE *ALGAE ASSAY - I C 5 0
1.2 % v/v
(1.0
>13 % V/V
(-1
TESTING CANCELED
- 1.7)
* = CONTRACT LAB DATA
