Document R218eZx1QEggw5bkpqq1j8bm7

N /A\ MM 2 i <38G CHEMICAL MANUFACTURERS ASSOCIATION \jel March 21, 1930 TO WHOM IT MAY CONCERN: We regret to report transcription errors found on pages 98 and 313 of the final report entitled " TwoYear Toxicity and Oncogenicity Study with Acrylonitrile Incorporated in the Drinking Water of Rats." The Chemical Manufacturers Association has sent you a copy of this report. Enclosed are new pages 97/98 and 313/314 that are to replace those in your copy of the report. Sincerely, Enclosures Leslie Anne Walton Secretary -V gc 3: 10 Formerly Manufacturing Chemists Association--Serving the Chemical Industry Since 1872. 1825 Connecticut Avenue. NW Washington. DC 20009 Telephone 202/328-4200 Telex 39617 'CMA WSH1 -97- TA2LZ IS CECS3 OBSERVATIONS OF Til:ooaocs ciangzs which veee statist:caht sic:n?icai-tit o:r"Fi:~ ::t female juts maintained foe. : tears cm grinning ;a~containing acetlomiieiiz Diagnosis Code 3-3 3-e 2E-7 F-Llc ?-l2d F-12 F-17 X-2 M-9a Observa cion Inteeument and Subcutaneous Tissue Subcutaneous tumor - ear canal (Cymbal gland) Tontue Turner or tumor-LDca proliferation of the tongue Uterus Endometrial polyp(s) Gastrointestinal Tract Gastric tumor - eunglandular region, focal papilloma <2 lm number Gastric tumor - nonglasdular region, focal papilloma >2 in number Gastric tumor - total cumber of rats with a primary tumor involving any part of the stomach Small Intestine - tumor (s) or diverticulum Nervous Svstea Pituitary enlarged, suggestive of a tumor Stain - focal changes in consistency or color sug- gescive of primary tumor Dose level (oof.) 35 ICO 300 tac. -- Inc. -- lcc . Inc. -- Dec. Dec. - ", -- lac. --*-> lac. Inc. Dec. -- Dec. " Inc, loc. Inc. ' Dec. Inc. Results are listad on the basis of whether the incidence race for each respective observation was statistically significantly increased (lac,) comparable to che control group (----- ), or apparently decreased (Dec.). (See Methods secclon) Data was analyzed using Fisher's exact Probability Test, ?<Q.Q5. Individual values for chese observations as veil as those which were not statistically significantly different are presented In Appendix Table 21. ^ 0 .3111 -93- TA3U n hiftopathologic :3st?.v.v::2t:s str.~u-f :? rr.sas ::: tzz cumc. nervous ststzm, ?zt:z~jj:'. tsr?.oiD. ahd atruiai. cla.*;bs -sas statistical:.'- sicNtFiCAim;: zzitizsztz ei tsxalz rats mac:,--li:;zd for : tius c:; diuti:;;: vatu cc:m:::;:;G acrtic^trcli Diagnosis Cede N'uaoer of racs tecrocsied curing the :ica perccd Indiancad Dose ppm in 2,0 0 ppm If ppm ICO ppm 300 ?pm o-? Moachs 0 0 0 1 H113 :ra?.voas ststzm Uinnttsc of tissues examined) 0 ppm 33 ppm 100 ppm 300 ppm "umber of rats with only a fecal 0 pom or aultifccal glial cell prolif arc- 33 ppm cion suggestive of early tumor in 100 ppm the central cervous syatam/taaber 300 ppm of rats in the greup 0 0 0 4 o/o' 0/0 0/0 1/1 H116 lumbar of rats with only a focal or multifocal glial cell tumor (astrocytoma)/cumber of rats in the group 0 ppm 33 ppm 100 ppm 300 ppm 0/0 0/0 0/0 0/1 :ui3,:ai5 dumber of rats with either a focal 0 ppm or multifocal glial cell pro 1 if era- 33 ppm tion suggestive of early tumor in 100 ppm the central nervous system and 300 ppm those with a focal or multifocal glial cell aimer (astrocytoma)/ number of rata in the group 0/0 0/0 0/0 1/1 PITUITARY GLAZED Clumber of tissues examined) 0 ppm 33 pom 100 ppm 300 ppm 0 0 0 H144.M147, Humber of rats with a pituitary :ii49 tumor (adenoma or carcinoma)/ number of rata in cha group 0 ppm 33 ppm 100 ppm 300 ppm 0/0 0/0 0/0 0/1 THYROID CLAUD (Humber of tissues examined) 0 ppm 33 ppm 100 pom 300 ppm 0 0 0 T101.T102 Humber of rats with a C-cali tumor (adenoma or carcinoma)/ number of rats in the group ' 0 ppm 33 ppm 100 ppm 300 ppm 0/0 0/0 0/0 0/1 ADRENAL GLAND (Humber of tissues examined) aii;,a143 "umber of tats with a pheachromo-- cycama (benign or malignant)/ number of tats in the group 0 ppm 33 ppm 100 ppm 300 ppm 0 com 33 rpm 100 ppm 300 ppm -n _a \> 0 0 0 1 0/0 0/0 0/0 0/1 7-L2 hen ens 1 1 3 13 1 1 3 13 0/1 0/1 0/3 2/13 0/1 1/1 0/3 3/13 0/1 1/1 0/3 3/13 3 12 0/1 0/1 0/3 0/13 \ 3 10 0/1 0/1 0/3 0/13 1 1 3 13 1/1 0/1 0/3 0/13 13-13 Moncns 11 13 IQ 23 11 13 20 23 0/11 2/13 1/20 2/23 0/11 2/133/20 12/23 0/11 4/13 6/20 14/23 'll 12 19 21 3/11 3/13 4/20 0/23 11 13 20 22 2/11 1/13 1/20 1/23 11 13 20 23 1/11 0/13 0/20 0/23 13-21 Months 14 30 21 1T Temiual Cumuiati y4 1 ? Result. 20 30 1 44 1 43 Q ^4 44 30 24 T^ 0/43 1/30 2/34 2/11 20 . 4 1 0 * 0/20 0/4 0/1 0/0 30 43 43 43 0/30 3/43 3/43 7/43* 0/43 11/30 16/24 3/11 0/43 12/30 13/24 11/11 . 1/20 3/4 1/1 0/0 1/20 3/4 1/1 0/0 1/30 17/43* 22/43* 24/48* 1/30 20/43* 4K.43* ^>3* 47 30 24 10 30/43 9/30 a/24 1/11 43 29 24 9 10/43 4/30 3/24 Q/il 43 20 34 9/43 3/20 1/24 0/11 20 4 1 0 11/20 1/4 0/1 0/0 79 47 47 44 44/80. 13/437 12/487 1/43* 20 4 1 0 10/20 2/4 0/1 0/0 73 i7 43 42 22/30 7/43d 4/43d 1/43 20 4 1 0 6/20 0/4 0/1 0/0 30 43 43 43 Wad 1/43* 0/43a Statistically significant Increase from control when analysed using Fisher's exact Probability Test, p<0.05. Individual values for chase observations as wall is chose which wara not statistically significantly different are presented in Appendix Table Z3. *. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ ................................................................... - -313 TA3LZ .4-25 CCjnc'f) HISTOPATHOLOGIC: DUGirOSIS AMD MCM3ER Or' TJKOStS TH TTMALZ RATS maiiciaimq ?oa. : TEARS OM OaiMIOIC VATZR comtaihimg ACRYLOMmilZ Diagnosis Code Humber at rats necrapsied during the tine period Indicated Dose ppm in H73 0 ppm 33 ppm 100 ppm 300 ppm Q-*4 Mantas 0 0 0 1 7-12 Months 1 1 3 13 13-13 Months U 13 20 23 19-24 Mentha 43 30 24 . 11 Terminal Cumulatlv 1 mi Results 1 20 30 1 4 43 I 1 4a 1 0 43 1 c-m K-107 U-110 s-m S-123 S-117 S-U5 S-116 CAMIOVASCSUA STSTZM (Cant'd) Heart (Coat'd) Metastatic secondary tumor (S-ill) MUSCTLOSKHLcTAL SYSTEM (Humber or tissues examined) Mecastatlc secondary tumor (S-Ul. X-132* IC-L97) NEHVOUS SYSTEM 3raia (Nucoer of tissues examined) Granular cell tumor Oligodendroglioma Pineal gland tumor virh extension into brain. malignant Metastatic secondary tumor (N-149, S-L52, L-L63) 3raln end/or Soinal Cord Pocal or multifocal glial cell proliferation suggestive of early tumor Pocal or multifocal glial cell tumor (astrocytoma) FC 311.3 0 ppm 25 ppm 100 ppo 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppo 300 ppm 0 ppm 35 ?pm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0/0/0 0/0/0 0/0/0 0/0/1 0/0/1 0/0/1 0/0/3 0/0/13 a 0 0 1 0/0/0 0/0/0 0/0/0 0/0/1 1 0 0 9 0/0/1 0/0/1 0/0/3 0/0/13 0/0/il 0/0/13 1/1/20 0/0/23 9 0 1 23 0/0/11 0/0/13 0/0/20 2/2/23 0/0/43 0/0/30 0/0/24 0/0/11 47 2 7 11 1/1/43 0/C/30 0/0/24 0/0/11 0/0/20 0/0/4 0/0/1 0/0/0 20 4 1 0 0/0/20 0/0/4 0/0/1 0/0/0 0/0/30 0/0/43 1/1/43 0/0/43 I I 1 1 77 I 6I 3I 44 I 1/1/30 0/0/43 0/0/43 2/2/48 I 1 I 1 0 0 0 1 0/0/0 0/0/0 0/0/0 a/o/i 0/0/0 o/a/a 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 a/a/a 0/0/0 1/1/1 0/0/0 0/0/0 0/0/0 0/0/1 7 l 3 13 0/0/1 0/0/1 0/0/3 0/0/13 0/0/1 0/0/1 0/0/3 0/0/13 0/0/1 0/0/1 0/0/3 1/1/13 0/0/1 0/0/1 0/0/3 0/0/13 11 13 20 23 0/0/11 0/0/13 0/0/20 0/0/23 0/0/11 0/0/13 0/0/20 0/0/23 0/0/11 0/0/13 0/0/20 0/0/23 2/2/11 2/2/13 1/1/20 1/1/23 43 20 24 7 1. o/o/4a 0/0/30 2/2/24 0/0/11 0/0/43 1/1/30 0/0/24 Q/0/U 0/0/43 0/0/30 3/0/24 0/0/11 7/7/4a 0/0/20 1/1/24 0/0/11 0/0/1 0/0/1 0/0/3 2/2/13 0/0/11 2/2/13 1/1/20 2/2/23 0/0/43 2/2/20 2/2/24 2/2/11 0/0/1 1/1/1 0/0/3 3/3/13 0/0/11 2/2/13 5/5/20 12/12/23 0/0/43 11/11/30 15/16/24. 9/9/11 20 30 4 48 3 43 ' 0 43 0/0/20 0/0/4 0/0/1 0/0/0 Q/o/ao 0/0/43 2/2/43 0/0/43 0/0/20 0/0/4 0/0/1 0/0/0 0/0/30 1/1/48 0/0/43 0/0/43 0/0/20 0/0/4 0/0/1 0/G/0 0/0/30 0/0/48 0/0/43 1/1/43 1/1/20 0/0/4 0/0/1 0/0/0 0/0/20 1/1/4 1/1/1 0/0/0 10/10/30 2/2/43 2/2/43 . 1/1/43* r- o/o/ao 5/5/43* 4/4/48 7/7/43* ' 1/1/30 3/3/4 1/1/1 0/0/0 1/1/30 17/17/43* 22/22/43* 24/24/43* Daea listed as total number of this type cusor/mimber o rats bearing this type o tumor/number of rats la the group. *Staelsticaily significant increase from control when analysed using ?iaher's Exact Probability Tesc, p<0.05. Apparenc decrease froa central, **n^ *-- -~-i - - _____ 14310 (Css::' t) HlSTOJArSOLCC" diagnosis and :rJM3I7. 37 T'JMOSS ON 770'ALZ 7-V7? ic.n::a roa : toa.is in Da::n-:r; ;c VAT37 Do:rtA::;t:;G AC7.X10NDD1DD-3 Diagnosis uJCtf Number of rats cecrsussed during the tine period indented Dose pea in s.,0 0 ppa 33 ?pa ICO ppa 300 ppa N-144 M-U7 M-149 N-165 3-103 3-109 ic-iqo MEP.VCUS r'STODi (Cant'd) Soinai Cord (Number of tissues examined) hecastatic secondary tumor . (1-133) Pituitary Gland (Number of tissues examined) Pituitary adenoma Pituitary gland carcinoma without Invasion of brain Pituitary gland carcinoma with invasion of brain Malignant Schwannomas Mediastinal neurofibrosarcoma MISCZLLANEQCS Metastatic secondary tumor involving intraabdcainai fat (H-109, X-197) Intraabdominal anaplastic carcinoma with widespread netascasis, origin undetermined. probable pancreatic exocrine origin tNTHACXANTAL Neurofibrosarcoma/fibrosarcoma 0 ppm 33 pom 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 3C0 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 33 ppm 100 ppm 300 ppm 0 ppm 35 ppm 100 ppm 300 ppm 0 ppm 25 pern ICO ppm 300 ppm 0 ppm 35 ppm 100 pom 200 ppm 0 ppm 35 ppm 100 ppm 300 ppm G*6 Men css 0 0 a - 7-13 Months 1 T 3 13 13-13 Months 11 13 30 23 Mss css ia ZG 24 -- 'erminal CjsuiicivO.esults 20 30 4 43 I 43 0 43 0 0 0 * 0/0/0 0/0/0 0/0/0 0/0/1 1 1 3 13 0/0/1 0/0/1 0/0/3 0/0/13 U 13 19 22 a/a/u 1/1/13 0/0/30 0/0/33 44 20 24 -- 0/0/43 0/0/30 0/0/24 0/0/11 20 4 t 0 0/0/20 0/0/4 0/0/1 0/0/0 73 43 47 47 0/0/30 1/1/43 o/o/ia O/C/43 0 0 0 1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 1 3 12 0/0/1 0/0/1 0/0/3 0/0/13 0/0/1 0/0/1 0/0/3 o/a/13 0/0/1 0/0/1 0/0/3 0/0/13 19 47 12 20 19 24 21 10 1/1/11 4/4/13 4/4/20 a/a/23 23/23/43 9/9/30 7/7/24 1/1/U Q/0/U 0/0/13 0/0/30 0/0/23 0/0/43 0/0/30 0/0/24 0/0/11 2/2/11 1/1/13 0/0/20 0/0/23 7/7/43 0/0/20 0/0/11 20 79 4 47 1 47 0 44 9/9/20 1/1/4 0/0/1 0/0/0 23/32/30 14/14/43, 11^43- 3d 1/1/20 0/0/4 0/0/1 0/0/0 1/1/30 0/0/43 0/0/43 0/0/43 1/1/20 0/0/4 0/0/1 0/0/0 10/10/30. 1/1/43* 1/1/430/0/43 a/o/o 0/C/G 0/0/0 0/0/1 0/0/1 0/0/1 0/0/3 0/0/13 0/0/11 0/0/13 0/0/20 0/0/23 0/0/43 1/1/30 0/0/24 0/0/11 0/0/20 0/0/4 0/0/1 0/0/0 0/0/30 1/1/43 0/0/43 0/0/43 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/0 0/0/0 0/0/0 0/0/1 0/0/1 0/0/1 0/0/3 0/0/13 0/0/1 0/0/1 0/0/3 0/0/13 0/0/1 0/0/1 0/0/3 0/0/13 0/0/11 0/0/13 0/0/20 1/1/23 0/0/43 0/0/00 0/0/24 1/1/11 0/0/20 0/0/4 0/0/1 0/0/0 0/0/11 0/0/13 0/0/20 0/0/23 0/0/43 0/0/00 - - 0/0/24 1/1/u 0/0/20 0/0/4 0/0/1 0/0/0 31 1 4 0/0/30 0/0/43 0/0/43 2/2/43 0/0/30 0/0/43 0/0/43 1/1/43 0/0/11 0/0/13 1/1/20 0/0/23 0/0/43 0/0/20 0/0/24 o/o/u 0/0/20 0/0/4 0/0/1 0/0/0 ^30 771/43 0/0/43 Data listed as total rusber at this type tumor/number of rats bearing this type of tumor/number Of rats la the group. ^Apparent decrease from control, not corrected for earl/ normality.