Document QxO24LwLqe4xoqRv034aroQv
LabOMtDryReport
InterimReport ofPrefiminaryData for 26 Week CapsuleToidcologyStudy withPFOS inCynomolgus Monkeys
Laboratory Report No. FAC17-TOX-030 Covance6329-223
Tafing Laboratory 3M Environmental Technology & SafetyServices
3M EnvirorunentalLaboratory FluorineAnalyticalChemistryTeam (FACT)
2-3E-09 935 Bush Avenue, St PaiL MN 55106
Laboratory Contact KrisHansen, Ph.D.
Bldg. 2-3E-09 P.O. Box 3331 St.Paul,MN 55133-3331 Phone: (651)77MOIS FAX: (651)778-7176
Requester Andrew Seacatand Marv Case
3M Company Toxicology Services
220-2E-02 St.PauL MN
PAGE 1OF 5
jA,Efyp7,RommENTALLABORA TORY REPORT,VO.F4CT-TOX-030
..........................-...-..-.-....
.................................................................................... ...
Tim sn* was dmpod toestablisahPFOS dietarym4ake levelatwhich thereareno observableadverse effem observedinCynomolgus monlwys.Testamnmls were doseddaflyby oralpvage vnthvanmis amounts ofPFOS inL-== withina Sda= mpm& lh= were ftm dose gmips and one groupofcontrol animals.Seraamples were coUecW fromaU teaanimalsatII ddkrentu= pointsam ft courseofthe 26-wwk study.Serasamplescouectedfrom thennimal wo sat totheEnvirommmW Lab and anab-Led quantitativfeolryperfluorow=e mffonate(PFOS).
Serasampleswereextracteudsingan im-pmmg c=mon pmcedum The cKn= were quanb=vely w*zed usingluotmon hqtudchmmatoFophy/elecuagraMylem aim spwmmetry (ERYMSMSI,E), andewuated versusancmcted =w. Analyticdaeltail=s availablienthez* bmder maintainebdy FACR The pre== orabsenceofkwwn flumocbeimcaclonmmum= and
metaboliteissddwained by blvcctkom
inalldo= gmum PFOS levelgsenwduy uxrcasedftmgh the26-wwk um powl A relawmlydramatic m=ase occurrebd"= thewwk 2-andthewe& 4 samplingmbwval Male and ftmle PFOS levels =dced tarlycloseltybmo= ft study Allmatrixspft mq)lcswerevnthmtheQC pidehm spemfied
by themedio&
Rigorousqualtycontromle== and 3M EwArmmental Labor=y Sumdzd opwatingProcedureswere followedinthepreparatioannd mmlym ofmmplm ao=lmg tD apphcablemethodsestablishae=drdmg tD Good LaboratoryPracticegsmdeb= Then resultssbmiltble =ddered prdnnmary and aresubjedto cbanp Dataptmted hereisthehie= Walky dataawlableatthis
SCMwnpies vme shippedtoft EwammnemW Lab by CovanceLaboraimy Samples=Iuded indw
reportw,erereceivedbetween 10/6/Nand3/2199.Envftmmj=d Labpersonnelinitiataecdhain-of-L@ Upon receipotfthemmpies.
lb= isno bdiduigb= a=m associatewdiththesemmples forLC/ESMSMS armlysis.
PAGE20F6
M EW;7ROMMENTAL LABORA TORT
REPORTHO. FACT-rOX-030
:A
..........................
AnalyticW methods FACr-M-1.0,E=action ofPotassiumperfluo0dummifol= orodur Amomc Fluaroch=ucal
Sur&cm% fromLTm forAnalymsUsingEPLC/EShE FACT-M-2.0,AnalysisofFlwrocl=mcalsinLim Exft= UsingHPLC-ElwftMraYMM
spectrometry
FACT-M-3. 1,E=action ofPotassiumPmtwmOmmmffOnatc OrOd= FlwrOctmlicalCC)MPOurL&
from Serum orOd= FluidforAnalysiUssingIHPLC-EIWUMMft*lm SPO=MCZY FACT.M-4. 1,AnalysiisfpotassiumpafluorooclmusulfonaotreotherFlwrochermcalon Senun orOd=
FluidUsingBPLC-Ek=osprayMm Spectrometry
Sample prepamtion- aqueousumple% HPLCIESMSMS: iOn-P&ilinefx,traction Analyteis==ted froma mxmle ma= withwn Wnng mgm [=abuwl ==on= hYdr0g= mdtft
(rBA)]inaofcDntroW mmm==L Tm Cabo= =gM mkcm* WPM mc)mc flwmch=calsOn= theamon-TBA parisbMI4 themalyu is=*rred intoanon-pc)Lmigrmuc solven(tmetbyl-tert-
W edw),&md, andM=SMUW inmethanoflorNS melym
EPLC/ESMSM: fordetaileqduahutivewoit inBPLC, an ahqm ofema ismlectadand pusod ftw& a rev=. phaseliquicdhromatographic column.Basedon theaffinitoyftheamlyu forthesmdormy phaseinft columnrel-dwtotheliquid mobilephase,theanalyu isretainefdora cbmactedsticamount oftime.For example,ina standard solutioPnFOS may cluteat8.0niii@ Retenfimtimesb"= a standarPdFOS solutioanndthe analytoe=acted from Fom&vater indo mabw werem=hed on theBPLC systemtowithin1%
FollowingMPLC sepamor4 ESMSNS puvtdesa rapidand a=nze me= foranalyzinga wide rangeof organicompounds,includinfgluo=hcmicals.EleWo*W, an imizatimtechniquuesedptm* for &-=Uon ofmolecularions,isg=auy qmated atrelativemliyldtempwm=. MolecWcs areionized, and a primaryim dwadm=c oftheM*te, 5 selectedT.IUSim IsWdmrdcd withhlgh-CnCW PS; mbmp= collmons=ate smalle=r=dary ionicfrap=ft uniquetotheF=wy ion,whichare
detecto& For example,forPFOS (C@Fl.,SOia)nalysisi,on499 isselecteadsthedwaMxiStic primaryiorlIbis ion isfrap=ted intootherjow suchas80 amu (conmvombn toS%), and230 arm (C3FsSO3).Each of thesesecondaryfrap=ts isdewed and canbe usedtochfferentiPaFtOeS fromotherconwm& that nugidhavethem= charactmoc 499anm pranaryion,butdiffoalchmnicalcompositionsand
secondaryionfiagnotaficp)anne=.
. . ..........
5.1 Calibration
A nud-ImL M=ed ma= canratlond=k standaridsanalyzeedvery5-10samplestomorutor mm=tal && Caltbmon die& gwdards= comph= ifmmm=td responsieswithin+/-300/o-f
theexpectedvalue. Quantitatioonfthetargetanalyteissbasedon linw regmwon analysi(swei&ed 1/.xi,fappropriaten)tihoef two extractedniatnxcurvesbracketlnegachgroupofsamples.Qu2Mtat'OnOfeach MlYte isbased
responseofone ormom speac dau&a ionsusingthemuldplemspmise-mor@toringmode ,ofthe
instrument
PAGE30FS
JMZVVIROMMENTAL LABORATOJTY RMJTTNO. FACT-TOX-030
5.2 Blanks
Twoe=mon blanku!w@m,ngwatearssurrogmatuemarct=ctedwitehacbhatcohfsamples.
AddidonaUy,two samplesofblankmaui-@,usedforthecmcted cabbiadoncanm am mmcted along with eachbatchofmmples and cmu
Blwft arecompliantifno targeztmlyw a ddectedabovethelimitofd&=aon fora Specifiscubu. In ttusz*, allblankswerecouphanl
5.3 Surrogates Tetm-hydroperflwmocmm affonateisused as a mnmgme m ft amlyw. momtomd tocon&m gm mmumenal fadwr.f,fne=ssary
Surrogateresponseis
5.4 Matrix Spikes
Duplicatematrixspikeanalym tiw-n alltargeatnabts vim pmtamd and mmbzed foronecontrol aumal Forscm ambms, rewvm= wen withinde acceptablre-m@pof70-130*/&Recovm= outmde thisrmp may be m-evahati
5.5 Laboratory Control Samples LabwatmyControSlample=s nota cmnpmentofthiswdy.
5.6 Sample Related Comments
ExnctW am= su*lcswei screenefdorothekrnownmetabolitoefEsT-FOSE-OHnd fbrPOAA No othermetabolitewsere &-uxui Ifncce&;M,exuam nay be scamed foram=* undmactcri=d nztabdlitumsinga fime-d-fligfbttmm mm spwmmew.
inalldm groupsP,FOS levelgso=auy m=scd throughft 26-wwk am pomt A relahveldyra=c m=ase o=mcd betweenthevie*2 andtheweek 4 smvlmg m=val Male andftwe PFOS IMIS uwyed fiwiycloselaymigh= theso*. AU mainx spdmmmples werewithmtheQC gmdebne sp=f3ed by themethod. A fidltableofresultissattached
niisreporrteflecthsemosta=um dataavailablaetthistm. Howe=, allnumbersshww be =wdemd tobe pmhnunary and aresubjectochange.
Samples and omractswillbe remmed forfumm cvaluanon,ifnecessary ntera
...........................................................
8.1 Attachment A: Graphicaland numeric surmnaryof averagePFOS levelsinsem 8.2 Attachment B: FulltableofresultfsorPFOS levelsinsera
PAGE4CF5
JA,EfMMONMZVTAL LABORA TORY PZPORTNO. FACT-=.Ojo
LisCagrmn,AmlyticCahlemiSt
Date
a,e, KrisHinsen,PILD.,St* Ditt=
Date
PAGE5OF5
('Ovanc. 19-223
Monkey Serum 6329-223
3(K)OO
250 (X)
200 ou
PPI)s as/mL (pp-)
13000
10000
lioou
000 (koW IK O.QmglkgtdmyGrow IF,D.Omg&gldoy
(koW 2K 0.02mg/kWdey
QVW 2F.0 02mWk#dmy GROW 3K 0-img4rWdey GroW 3F.0 5m&Wdxy OmW 4K 2mg*Wt
Dose Level
Series I = Baseline Series2 - Week I
Series3 = Week 2 Series4 = Week 4 Series5 = Week 6 Series6 = Week 8 ETS41-03 ExoeVlasion70
Series 7 = Week 12 Series 8 = Week 16 Series9 = Week 20 Series10 - Week 24 SeriesI I - Week 26
mm223.xls
study Ps.-dudNwnbeqTwi Sutat@e)
ki,l,.
CoviLncr t9-2LJ
26W@A t'apsuiTsoxicityS" T-6295 KPF'US) Monky.-k.
withPR)S in(ynomi"
Monkeys
Sample Datm
Ntunkey
Serum Group D..t
method tilk Method tilk Matrix llik kist.ll.ilk
2 @o ppb
0 oil,elf-id.)
ur,)upVl.V..@.11_,.,X,k,,day
G l.up2
g/dy
tiroup2F,002.,S,kl,day
(itoup 3M. 0 St,,g'#k/day
L;t@p 3F. 0 5.#,k g@dy
Grmp 4M. 2inglkg/day
Otmp 4F,2n,gA#,dy
S.Mpit N
02/()9/9R9WW BaBolin.
A, I
1120 Blk-I 112UHik-7 -GbbSoo.-Blk- I k.bbi 4@ Btk-I
-M[)L Ml)[.
117% MDI, MI)L
TRDT
MDL
Mt)L
-ISD Std. Mv.
021051" wi Weak I
AversisIF" vlvml
@MI)L
IMDL
-MI)L
MDL
5%
ML)L -7P=
<MDL
123%
'mr)L
<MDL
--I-OT--
1.19
I MI)L 1 _463
<M DL
262
<MDL
-2
.11"
MDL
-MDL
0*/.
-MDL
@MDL
--FIBJ-
0,137
0568 196 338
02/09199 lc7p
Wmk 2
Average
RSD
r"
Std. Day.
w/mL
03/02199gAtVRWW
Week 4
Average RFOS eaftl
RSO Bt& Dow.
@MDL
MDL
--@<D,,LM0D,L%L
@MOL
<MDL
MDL
-1201/.
<MDL
@MDL
--=
137
2%
@MDL
MDL
--O-TU-
0,120
.
6,73
33@o5
27
116 ;13
<MDL
96%
LOQ
@LUQ --T99-
425
20.6 1 I1 ;v
6
<MDL
1%
,
3%
<LOQ
@LOQ
--o-T2o-
03963
'1643 2140
44@944
01110/99 IAH Wnk 6 Average RFOG vWinL
RSD gt& Day.
MDL
IMDL
@MDL 103% 98%
<MDL
@MDL ---r 0=
4,63 255 23 5 Its
112
-MI)L 4% 2%
<MDL
MI)L ---o r3T--
0.520 706 2" lot 9a
0YOV99 R W"k a
Average PFOO LVML
kwL
MDL 7 VI. 77%
MDL
<LLIQ =16
638 324 300 136 133
Somple DaLs
Nlonkey
Serum Group D...
M.dd 1311=.Illk
Maum Hik 2 50 ppb
G.@:p2;TIF..00t) l,ii,dy
02n%gfkgldy
,,",.-uppI3FU..0.02n,g/ig,day
3mg/Reldy
Ot-p 3F,0 5milkildly Group 4m. Zmx,ts-yl@
'I
it,@up4F@ 2mgto,day
simple 0
3112/" SAIVRWW Week 16
Average rvos Ivmt,
1120 Bik-2
-kibb,Stm-Dik
I
Rabbit Sm Hil
MI)L
kiDL 99% 106% LOQ LW 140 131
lot
52 6 235
RSD BK Day.
3/15/99 ICP/BMI Wmk20
Average r" ogiml
Rai) SUL Day.
@MI)L
@MDL
MDL 4.,. 16% LOQ LOQ -105 3@725.37,
ML)L
t- .9-,
1
78%
o otri-
00690 irr
is 3
<MI)L
MDL 11% 2% --fo-F6y-00065
223
1/1&*99 RWWISM Week 24
Average r" ug/mL -
RAD It& Do.
-
<MDL
<MDL
-MDL 89% 90% 00612 00544
<MDL 9% 2%
00109 0.0094
163
0942
504
72 5
114 1
754
9,04
1
0
V16/99 RWWM"
Wffk 26 Average rros ulVmL
RSD 84& Day.
.
<MDL
<MDL
<MDL 95% 96%
00623 00729
19's 16.4
ioi
933 21r 214
<MDL 17% 1%
00143 00159
1.76 1.77
---TrT-
13@S -- 4Tr
277
E'TS N 5 0 Ex-I V@@ 7 0
FACT TOX-030 Covance# 6329-223
snob, ProductNumber(TestSubstawc). Matrix: Method/Revision: AjWytiul EQuipnww System Number. lnsaiinwwSoftwamevenion: rolenum: R-Squwed ValueSlope: Y-Inftwqw. Dateof ExtractiontAnalyst: Dateof Analysis/Arajysr.
I)IftofDau ReducnvWAaWyst:
26 Week CapsuleToiLicitSytudy withPFOS inCynonwlgw
'r Monkey Sea FACT-M-3.1 & FACr-M-4.1 Soup 020199 MazzLynx 3.1 See AtU=bnxus See AttachumM See Attwbnwm See Atuwhmem 02109M RWW 02113199 MEE 02/ISM KJH
Monkeys
BASELDM MONKEY
Group Dan hfedbod Hik Method Blk MatrixRik Main% Blk OC - 250 ppb Group 1 Control 0-0mpwday
Group 2 LOW-Dose 0.02nipwday
Group 3 NUd-Dow 0.5ntg/Wday
Group 4 Ffigh-Dose 2.0 mg/Wday
SERA
sampit#
H20 8 Ik-I M20 Blk-2 RabbitStraRik-I RabbitSetsDik-2 MKS02059-MS MKS02059-MSD 105508M 105517M 105319M 103520M 105526M 105527M W5529F 105330F IOS531F 105535F 1055"F 105549F 105513M 105314M 105515M W5516M 105521M 105525M 105537F 105541F IGS$43F 105546F 105547F 10555OF 105505M 105510M 10551&M 105523M 105524M 105528M 105532.F 05539F 105S39F 105545F 105S48F 105552F 105506M 105507M
mssmm 105511 m 105512M 105522M
'100555'334'FF
Sample Data
rrw com ng/mL <MDL -DADL <bfDL <t4DL 298 293 1.64 1.68 3.42 1.01 1.97 1.83 2-" 1.50 2m 1.22 1.95 3.57 2.93 1.94 0.94 2.94 2.06 2.23 1.69 2.54 Z93 2.80 2.03 1.85
3.40 1.41 1.96 1.31 zis 2." 3@40 257 2.47 3.53 3.95 2.35 2.50
3.CY7
138
2.99 Z93 3.02 2-11 Z83
Pros ReponW
<MDL <MDL @CMDL -ddDL 120% 114% <bfDL @dWL dADL d*WL -L%(DL <MDL -cML -d4DL <MDL @DADL 44DL <MDL
-DADL <MDL <MDL -d4DL <)6ft)L @CMDL -DADL <MI)L <NFDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <PADL <MDL CMDL <MDL <MDL <MDL cMDL <MDL <MDL <MDL
<MDL
<MDL <MDL <.MDL <MDL CMDL
Avmge FFC)S U&hnL
<MDL
Q%WL
117%
RSD Std.Dev.
<MDL <MDL
5%
<MDL
<MDL
<MDL
<MDL--.
IOADL
CMDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
105536F 10554OF 105542F 105551F Unut ofQuantica(io(nLOQ):25 nVmL Method DetectionUrr@t(MDL): 12ngtffLL
3.13 3.70 227 3*77
DateEntemd/By: DateVcrifm& By:
<MDL
<MDL <MDL <MDL C)2/17199LAC 03r-3M OML
<MDL
<MDL
PFOS - Pernuoromm=uifanate
FACT-M-4. I ExcelVersion7.0
scru'-12.1.xis
7 t-
3rW)9
FACT TOX-030 Covance# 6329-M
Smdr. ProductNumber(TestSubstimle): Matrix; MethodfRevition: AnalyticalEquipmentSystem Number lummat SoftwamNersiorv Fiknanic: R-Squamd Vaiw. Slope:
Y-Inwmept: Dam of ExtmctioWAnalyst: Dateof AnalysWAnalyst: Due of Data Reductiom/Analyst:
26 Wak Cspmle ToxicityStudywithPR-OS inCynomolg-
T-6295 (PFOS) Monkey SCM FACT-M-3.1 & FACT-M4.1 Madeline041098 & Arwha 062498
ManLynx 3.1 See AnadurAm See Atu4aneffls See AnwJmmu See Anchumgs 02MS199 SAH 02MM.oVlotgg.GV12199.02117199 HOI/DRB 02/10",ovl6fflg.02/26/9H9OJIDRS
Sample Data
Monkeys
WEEK
I MONKEY Group Dan
SERA
Sample 0
Medtod glk Method Bik i@fahiOlk MatrixBik Qc. 250 ppb
Group 1 control 0.0n4Aqlday
Group 2 Low-Dose -eirmSAg/day
Group 3 Kd-Dose -0.*mgWday
Group 4 Ifigh-Dose
nigAcVday
H20BIk-I H20 Bik-2 -ii;'bbis..-Blkl pabbitSen Rik-2 MKS02059-MS MKS02059.MSD
105508M 105517M
ID5519M 10552M iossum 1055VM 105529F 10553OF 10553 1F 105535F 1055"F 105549F
105514M 105513M 105516M 105521M 105537F 105541F 105547F
10555OF 105505M 105510M 105318M 105523M 105524M 105528M 105532F 105539F 105539F 105345F 105WF 105552F
105506M 105507M 105509M
ID5512M 105522M 105533F 105534F 105536F 10554OF 105542F 105551F
Unit ofQuanutauon(LOQ): 25 ng/mL Method DetectionLimit(MDL): 12ng/mL
RFOS coom
ughal <MDL <MDL <MDL CMDL
305 304 O.OD O.OD O.W O.OD 0.00 0.00 0.00 O.W O.OD D.W O.W 0.00 495 383 519 677 694 464
$91 519 297 2D9 342 221 318 413161 257 249 194 232 i95 665 411 351
492
711 539 564 451 393 419 749 377
PFOS Re"rtad
uzhnl -MDL cMDL dADL <ML
23% 1123% <MDL 44DL <MDL dADL 4d#ML <MDL DADL <MDL <MDL <MDL <MDL <MDL
0.990 0."7 1.04 1.35 1.37 1.16 1.16 1.04 5.74 5.23 6.94 4AI 5.30 6.89.5.39 5.15 4.13 4.61 4.65
3.90 26.6 27.4 23.4
24.1
29.5 26.9 29.2 22.6 26.2 20.9 29.9 25.1
Averar PFOS ugknl <i%(DL <MDL 123%
elADL
<MDL 1.09 1.19
5.74
4.63
26.2
25.5
Date EnteredIBy: 02110199.03102/99LAC
DateVehfied/By: 03r-V" GML
RSD Sid.Dow.
<MDL cMDL
0%
<MDL
16.9 0*193 11.6 0.13
17.0 0.976
12.3 o.5
7.49 1.96
13.3 3.39
PFOS . Perriuorooctanesulfonate
FACT.M4@l ExLelVersion7.0
scra')"3.xls
3r-611)1)
FACT TOX-030 Covance# 6329-2M
Study: ProductNumber(Test Substance): Matrix: Method/R"ion: AnalyticaElquipment System Number. lnstmnwntSoftwafe/Vemorr Filenum: R-Squamd Value: Slope: Y-ingemept Dateof ExuactiontAnalyst Dateof Aaaly&WAiWyst: Dateof Dua ReductioWAnalyst:
26 Week CW3uic ToxicityStudywithPFOS inCynomigus
T-6295 (PFOS) monkey Sera
FACT-M..!.[& FACT-M-4.1 Madeline041098 & Armba 062499
MussLynx 3.1 See Anachm=U See AttachffmU See AttachavAn See Anachmmts 02M9)99 JCP 02/15/99.02t22199HOJ 02119199.02123/" HOJ
Sample Data
Monkeys
WEEK 2 MONKEY Group Dow
SERA Sampk
Method Btk Method Blk
H20 Bik-I H20 Bik-2
Mani, Sik MatrixBlk
R-hbitSeraBIk-I RabbitSera Bik-2
QC - 250 ppb
MKS02059-MS MKS02059-MSD
Group I
control 10-0m&Wday
M5509M W5517M 10551,9M 105520M
105526M 1055M
105529F 10553OF
M5531F
105535F 1055"F
105549F
Group 2 Low-Dose 0.02 mSAWday
105514M iossism 105516M 105521M 105537F
105541F 105547F
10555OF
Group 3 Nbd-Dose 0.5 mgAcWday
105505M 105510M 105518M 105523M 105524M
105529M
105532F
105538F 105539F
105545F
1055UF 105552F
Group 4 High-Dow 2.0mglklvday
105506M 105507M 1055MM 105511M
105512M
105522M 105533F
1055.UF
105536F
10554OF
105542F
I 105551F
LirruotfQuanutationiLOQ): 25 ngiml Method DetectionLimit(MDL): 12 ngImL
PFOS Cone.
PFOS Rq-ried
Avemge PFOS
nWmL
RBAUL
ugftl
<34DL <MDL <MDL 4dADL
<MDL -dWL <MDL <MDL
<MDL -ANWL
293
118%
30D
121%
120%
4.09
dADL
3.07
40L
4,53
40L
2.08 2.M 1.8-9
<MDL 4d@WL
--CMDL
<MDL
0-520 1.02
44DL @dmL
1.90 3.12 0.380 -0.810
IDML <MDL dADL -<MDL
<MDL
575
lis
513
1.28
492
1.49
425
1.42 -1.37
446
1.49
722
1."
5v
1.32
530
1.22
137
227
7-58
301
6.01
350
9.75
295
5.69
195
7.78
394
?e68
7.25
173
5.75
416
8.33
232
5.90
329
6.59
320
9.01
295
5.90
6.73
747
37.3
626
31.3
746
29.9
706
28.2
976
39.1
S"
35.9
33.6
776
31.1
650
32.5
598
29.4
546
24.3
372
24.8
569
22.7
27.5
Date EntemdtBy: 02ra", 03102199LAC
Date VairtedtBy-. 03a3/99 GML
RSD Std.Dev.
c.%IDL DADL
2%
<MDL
<MDL 7.63 0.104 9.79 0.120
16.1 1.16
17.2 1.16
13.1 4.41
14.8 4.05
PFOS - Perfluorooctanesulfanate
FACT-M-4.1 ExcelVersion7.0
scra223.xb
3r-6199
FACT TOX-030 Covance# 6329-223
Studr. ProductNumber(Testsubuaft-e): Matrix: Method/Revition: AnalyticaElquipnem System Number.
InstrumentSoftwamtVeruou: FikutmR-Squamd Value: Slope: Y-h=cept: Dateof ExuactiorVA=Iyst: Dateof Analysis/Analyst Daleof Dam RaWcuoulAmlyst:
26 Week CapsuleToWcity Study -IM PFOS inCywmlsus
T-6295(PFOS) monkey Sent ETS41-4.0and ErS41-3.0 Madeline041098.Amelia 062498.& Soup 0201" MAULynx 3.1and 3.2
See Anadmimmu
SeeAnadumM See Attachments
See Attachments
03AM" SAHVRWW
03105/",03=M. 03/25/99HOJ/MEFJDRB
03MM.03MM
KJH/DRB
Sample Data
Monkey&
WEEK 4 MONKEY
SERA
Group
Sample
Dan
Method Blk Method Blk
H20 Olk-1 H20 Bik-2
MatrixBlk MatrixBlk MatrixBik MatrixBik MjLmxBlk MatrixBlk
QC -250 ppb
RabbitSem Bik-1 RabbitSera Bik-2 Monkey Sem Blk-I Monkey Sem Blk-2 Monkey SentBlk-3 Monkey SentB&-4
MKS03029-MS-1 MKS03029-MSDl
OC -250 ppb
MKS03029-MS-2 MKS03029-MSD-2
Group 1 control mVWday
105509M W5517M 105519M W552(*A
10552ILM 105527M
105529F
10553OF 105531F
105535F
105544F 105549F
Group 2
105514M
Low-Dwe 0-02mVWday
105515M 105516M 105521M
105537F 105541F
105347F 1055SOF
Group 3 Nhd-Dose 0.5 mgAWday
105505M 105510M 105519M 105523M
105524M
105528M-
105532F 105539F 105539F
105545F
105549F 105552F
Group 4 High-Dose 2-0-g/Wday
105506M iossm 1055mm 105511m
105512M iOSS22M
105533F
105534F
105536F
1053QF 105542F
1
10555 1F
UrWt ofQuandtadon (LOQ): PFOS 15.2ng/mL
Method DetectionUn@t (MDL): PFOS - 4.76ag/mL
PFOS CONC. nghol
0 0 0 0 7.21 7.17 7.26 6.32 253 250 242 235 5.43 6.76 7.91 6.11 6.73 I." 6.63 6.11 9.19 B.17 9.96 8.74
109 172 196 101
196 153 221 163 63.7 64.6 101 110 96.3 95.7 41.4 117 35.9 46.7 78.3 55.9
lis 27.2 27.3 24.2 29.0 30.9 35.2 36.3 24.0 834 29.4 15.0 Date EntemdlBy: Date VcrirsedB/y:
PFOS Repoftw
ushnl
Avelor PFOS ughul
<MDL <MDL
<MDL
<MDL
<MDL
<MDL
4.OQ
CLOQ
<LOQ
<LOQ
<LOO
<LOQ
102%
iois
101%
98%
"S
96%
<L.OQ
dA)Q
<LOQ
<LOQ
<LOQ
<LOO I <LOO
4.OQ <LC)Q 4.OQ <LC)Q <LOQ
<LOO
3.59 3.43
3.91
5.03
3.99
4.65 3.93
4.43
4.09
4.25
21.2 21-5
25.2
22.0
19.3
23.9
--22.2
2D.7
2.3.3 17.9
23.4
19.6
19.6
20.6
295
90.6
69.4 W.5
96.6
103
119
176 lal
90.0
83.4
97.8
75.2
116
0.4M&099.0.'4/2619L9AC
03123/99GML
RSD SI& Dev.
<MDL dADL
<LOQ
CLOQ Is
<LOO
d.OQ 18.1 0.720 8.53 0.363
9.47 2.10
11.4 2.34
73.9 -87.9
42.7 494 PFOS - Pvfluorooctanesulfonate
ET.S-8-5.0 ExcelVersion7.0
sem2l-3.xls
3r_Wl)
FACT TOX-030 Covance# 6329-223
Study:
ProductNumber(rcst Substance):
Matrix: MctbodfRevision: AnalyticaElquipnmt System Number. InstrumentSoftware/Venion:
Filmme: R-Squamd Value: Slope: Y-Inlcrccpt: Due of Exuamon/Analyst: Dateof Analysis/Analyst Dateof Data Reducuon/Analyst:
WEEK 6 MONKEY
Gnmsp D@
SERA
Sample
Medbod Blk Method Dik
M20 Bik-I H20 Dik-2
Mavix Bik MatrixBik Mania Bik
RabbitSam Blk-1 RabbitSam Rik-2 Monkey Sem Blk-I MoolD.-yScm Bik-2
Monkey Sam Bik-3 Monkey SeraBlk-4
QC -250 ppb
MKSD3109-MS-1 MKMIMMSD-1
Q r--= M b
MM31096MS-2 MKS03109-MSD-2
Group I control 0.0nil/Wday
10550am 105517M 105519M 105S20M IDSS26M
105527M
105529F IOSS30F
105531F
105535F 105544F
105549F
Group 2 Law-Dose 0.02ir4,Wday
105514M lossi5m 105516M loss2im 105537F
105541 F
105547F 10555OF
Group 3 Mid-Dosr O.S mg&Wday
105505M
105510M 10351SM 105523M IOS524M
105528M
103532F 105538F
105539F 105545F
IOSS49F
105552F
Group 4 High-Dose ZO mg/kglday
105506M 105307M 105"9m 105511 m
105512M 105522M
105533F
1055UF
105536F
10554OF 105542F
1
105551F
Un@t ofQuant3tatio(nLOQ): 25 ng/mL method DetectionLinrL(iMtDL): 12 ng/mL
PFOS - Pcrfluorooctanesulfonate
26 WeckCapule ToxicitySbAywidi PFOSiRCYROnkOIXUS
T-6295 (PFOS) Monkey Sera ETS414.0 and ErS-9-5.0 Madeline041098,Aniclia062498.& muslynx 3.1mW 3.2 SeeAttadmon SeeAnubnmu See Attachments SeeAnaancos 03110M SAH 03/12199.03821199MEEn3RB 03111IV", 03/24199LACN)RB
Soup 020199
Sainple Data
PFOS Cmc. ox/mL 0.00 7.74 0.00 4.19 2-56 2.55 1.93 0.5 264 253 243 249
O.OD 0.00 0.00 1.60 0.61 9.49 L56 3.39 4.24 0.00 0.00 3.54 36.6 33.1 32.5 41.8 42.7 45.7 36.4 32.7 97.4 56.1 66.9 61.9 727 61.6 61.2 94.5 61.4 94.5 61.3 67.0
192 165 230 234 211 203 232 166 179 21-7 203 205 DateEmmd(By: DateVaificdB/y:
PFOS Repoud
uthul
Average "US ughnl
-clOppb <10ppb <10 ppb <10mb
-clOppb <10 cob
<10 ppb <10ppb
<10ppb <10mb
<10 ppb
106%
101%
103%
97%
IOD%
"%
-G%IDL <ML
<MDL
44DL <ML <MDL
<MDL
<MDL
<MDL -O%IDL
<MDL 4ADL <MDL
<MDL
4.58
4.13
4.06
5.23
4*50
5.34
4.57
4.5S
4.08
4.63
26.2 28.1
25.1
23.2
27.2
23.1
25.5
23.0
23.6 23.0
28.4
23.0
2D.1
23.5
114
103 115
117
132
127
lis
116
104
112
111
127
102
112
03/19/99.0312419L9AC
0
MOWECYS
RSD SUL Day. <10ppb <10 ppb <10 vpb
4% 2%
<.MDL
<MDL 11.9 O@537 11.2 O.S20
9.09 2.06
11.4 2.68
8.54 10.1
7.9)0 B.u
r;TS-9-50 Excel Version7.0
sera,-p23.xis
3r-6/99
FACT TOX-030 Covance# 6329-223
Smdr. productNumber(Test Substance):
26 week Capnic ToxicityStudy%ilbPMS inCYBWWISU MOD" T-6295(PFOS)
mawll: Mctho&VRevision: AnOyticalEquipment System Number. InstrumentSoftwam/Version:
Fikmnic: R-Squamd Value:
s4m: Y-LwmrcW: Date ofEitmction/Analyst: Date ofAnalysis/Aiudyst: Dote ofData ReductiontAnalyst:
Monkey Sen ErS.".O and ETS-9-5.0
Soup 0201" MUSLynx 3.1and 3.2 See Attachments SeeAimchnzm See Anacbfnenn See Anschnmu 03103199RWWIJCP 03/OSM. 030999.03/25199HOJ/MEEIDRB 03=M, 03115n9,03MM DRB
Sample Data
WEEKS MONKEY SERA
Gnmp D@
Sample
PFOS Can& nghal
RqPoFOrSsd wt/ML
Ava PFOS ULtinL
RSD SUL Dev.
Method Bik Method Sik MatrixBik MatrixBik MatrixBlk MatrixBik
H20 Bik-1 H20 Blk-2 -i6bbit ScraBik-1 RabbitSem Dik-2 Monkey SeraBlk-I
Monkey SeraBlk-2
QC -250 ppb
MKS03039LMS-1 MKS03039-MSD-1
QC -250 ppb
MKS03039-MS-2 MKS03039-MSD-2
QC -250 ppb
MKS03039-MS-3 MKS03039-MSD-3
Grv" 1 control 0.0mg/Wday
105509M 105517M M5519M 105520M 105526M
105527M
105529F 10553OF
105531F
105535F
1055"F IOSS49F
Group 2 Law-Dose 0.02nig/Wday
105514M lossism 105516M 105521M
105537F 105541F
105547F 105550F
Group 3 Wd-Dosc 0.5mg/Wday
105505M 105510M 10551am 105523M 105524M
105528M
105532F 105538F
105539F
105545F 1055"F
105552F
Group 4 High-Dosc 2.0mglktlday
105506M 105507M 105509M 105511 M
105512M
105522M
105533F 1055'.t4F
105536F 1055QF
105342F 105551F
LirruotfQuantitatio(nLOQ):25 nfiml
Method DetectionLinut(MDL): 12nVmL PFOS . Perfluarooctantsulfonate
0.00
<MDL
O.DD
<MDL
<MDL
<MDL
0.00
ch(DL
O.W
di4DL
<MDL
CMDL
O.W
DADL
O.CD
44DL
dADL
4ML
174
70%
191
77%
73%
9%
205
93%
185
75%
79%
10%
181
73%
203
92%
77%
11%
9.43
4dADL
7.68
d4DL
12.0
<MDL
9.03
dmL
IIA
<MDL
28.8
0.0490
-@@MDL
<MDL
16.0
<LOQ
18.3
<LOQ
ILO
<LOQ
14.5
<LOQ
9.46
4IDL
15.0
<LOO
<LOO
<LOO
37.2
6.20
33.8
5.63
47.1
6.73
8.98
34.4
6.99
6.36
0.365
49.2
7.03
39.0
6.51
40.1
6.68
11.8
37.1
5.30 1
6.39
0.752
122
34.8
92.9
27.6
94.1
31.4
91.6 lie 107
30.5
39.4-
12.7
@6.7
32.4
4.1
86.1
34.5
106
30.3
82-6
33.1
122
30.5
69.4
27.9
12.7
59.5
23.9
30.0
3.92 1
122
157
93.9
107
117
128
$9.8
115
115
147
16.7
127
163
136'
22.8
l(Y7
138
98.1
151
100
128
102
131
113
145
11.1
99.2
109
133
14.8
Date EnKie&By: O.VOBM. 03/24/990.3/26M LAC
DateVerirjc&By: o
ETS-8-5.0 ExcelVersion7.0
scra223.xb
3r26199
FACT TOX-030 Covance# 6329-223
Study: ProductNumber(Test Substance): Matrix: McdwdRtcvision: AnalyticaElquipn=t System Number. instrumentSoftwweJVersion: Ftlenanic: R-Squaied Value:
shnm: Y-IrAercept: Dateof ExtmctiordAnslyst: Dateof Analysis/AiWyst: Dm of Dam Redwdon/AnWya:
26 Week CapsuleToxicitySnidy withPFOS
T-6295 (PPOS) Monkey Sem ETS-84.0 and ETS-8-5.0 Soup 0201" ManLynx 3.2 See Attachnods See Aitachmems See Attachments See Attachments
03M3199 RWWIJCP 3MW".03/10/".03/25)99 DRB 03115M. 03/24t99.03f26099DRB
inCynornolgusMonkey&
Sample Data
WEEK
12 MONKEY
Group Dan
SERA
smpie
PFOS Come.
PFOS Rq-ud
09ARL
Avemp rfos ughnl
RSD Std. Dev.
Method Olk Method Bik MatrixBlk MatrixRik Mmhx Blk Matrix Blk
H20 Blk-I
F
H20 Bik-2
R&Wt SentBik-I
RabbitSent Olk-2
Monkey scm Bik-I
M*Wm SeraB*-2
@D0O7.0W00 O.W O.W O.W O.W O.W
CMDL <MDL cMDL <hmL <MDL <MDL
<MDL @64DL -MDL
<MDL <MDL <mr&
QC: -250 Mb
mr-WM9-ms-l
174
MKS03039-MSD-1
191
70%
77%
-"%
QC -250 ppb
MXS03039-MS-2
2DS
83%
MXS03039.MSD-2
its
75%
79%
10%
QC -250 ppb
Group 1 Contml 0.0nig&eday
MKSOM9-MS-3 MKS03W9.MSD-3
W5509M W5517M 105519M iossm W5526M lossrm
181 203 30.9 26.9 32.8 12.7 21.3 32.6
73% 92% 0.0476 0.0537 OD$47
<LOQ 4d.OQ oosn
77%
11%
0.0533
7.67 0.004D9
W5529F 10553OF 105531F W5535F tD55"F 105549F
13.2
dmQ
18.4
4MQ
21.1
4.OQ
11.2
<MDL
202
4.OQ
25.6
0.0433
<LOQ
<LOQ
Group 2 Low-Don 0.02-StWday
105514M 105515M 105516M 105521M 105537F 105541F 105547F 10555OF
44.7
9.278
47-5
L636
30.6
1.72
49.1
10.0
61.3
9.02
37.5
7.49
39.6
9 43
52.0
-7.7* 6
8.59
9.92
0.766
11.2
B042
0.945
Group 3 Mid-Dose 0.5mg/Wday
10550M 105510M 105518M 105523M 105524M 105528M W5532F 10553&F 105539F IM545F
105USF 105552F
143
50.0
135
49.0
133
42.1
98.0
".0
its
47.1
15.3
79.2
31.7
".0
6.73
B6.9
32-2
94.2
41.0
101
34.7
109
40.2
79.6
28.9
14.3
77.4
30.9
34.7
4.97
Group 4 liigfl@Dow 2.0 mg&g/day
105506M 1055" 105509M 105511M 1055 12M 105522M
103
144
$6.4
133
116
154
$3.9
124
149
209
19.6
as.0
ISO
152
29.9
105533F
1()9
158
1055.7AF
lw
143
105536F
63.6
122
105.WF
36.o
140
105542F
129
164
io.o
105551F
114
146
145
14.6
Uriiiotf Quancitatio(nLOQ): 25 ngirnl mcdkod Detectiounniit(MDL): 12nsiml
Date Entem-cVBy:03=M, DateVerifiedB/y: 0
03/24199.03r26/9L9AC
PFOS - Perfluorooctanesulfonate
f:Ts-8-5.u ExcelVersion7.0
scra223.xis
3126/99
FACR TOX-030 Covance# 6329-2M
Study: ProductNon6er(TestSubstance): Matrix: McdmcVRcvisiow. AnalylicaElquipnm System Number
tuffumat Softwardvefwon: Fikwm: R-Squaied Value: Slope: Y.Intercqx: Dam of Exwacdon/Analyst: Date ofAnalysis/AaWyst: DateofDan RW=on/Analyst:
26 Week CapsuleToxicityStudy withPFOS inCynonwlgus Mooker
T-6295 (PFOS) Monkey Sem ETS-9-4.0and ETS-9-5.0
Soup 020 1 MauLynx 3.2 SeeAttachniM See Attachments
See Attacbments See Attachments 3/12/99SAHVRWW 03/1SM. 03119/99 DP B 03116/",03/24t99DRB
Sample Data
WEEK 16 MONKEY Group Dm
SERA Sample
Method Btk
H20 Blk-1
Method Rik Me= Bik MatrixDik MatrixBlk MatrixOlk
QC - 250 ppb
H20 Blk.2 RabbitSera Blk-I RabbitSaa Blk-2 Monkey Sem Sik-I Monkey Sem Blk-2 MK=129-MS-1 bWM3129-MSD-1
QC -250 ppb
MK=129.MS-2 MXS03129LMSD-2
Group 1 contmi 0.0 mSPAoday
10550am 105517M W5519M 105520M W5526M
105527M ---
105529F 10553OF
ID5531F
105535F 1055"F
M5549F
Group 2 Low-Dose 0.02mglkglday
105514M mssi5m 105516M 105521 M
105537F 105541F
105547F
10555OF
Group 3 hbd-Dose 0.5mlAg/day
105wm 105510M 10551&M 105523M
105524M 105529M 105532F
10553&F
105539F
105345F
105548F
105552F
Group 4 HiSli-Doze 2.0 mg/kg/day
1055MM 105507M 105509M 105511 m 105512M
1()5523m--
105533F 105534F
105536F
105-WF
105542F 105551F
Limitof Quanutation(LOQ): 25 nVmL
Method DetectioUnrrit(MDL): 12al/mL PFOS . Peffluorooctanesulfonate
PFOS Conc.
nghal 0.00 O.OD O.OD -0.00 36.21 21.43 280 269 269 316 21.5 20.4 21.1 20.7 19.9 39.435.5 25.7 21.5 9.57 11.6 28.9 77.7 47.3 91.7 83.1 76.4 69.2 66.9 53.9 222 330 379 326 475 362 252 279
347
323 32.1 241
273 257 2% 269 2% 254
245 1% 184 259 231 191 Date EntcmdtBy: Date VerifiedB/y:
PFOS Reported
99ftLL
Atesur Pros agiml
<MDL <MDL
DADL
<MDL <MDL
-DADL
0.03621 0.02143
101%
oons
97%
99%
97%
116%
106%
<LOQ
ELOQ
d.OQ
d.OQ
<LOQ
0.(V767
<LOO
0.0546
<LOQ
<LOQ
<LOQ
<LOQ
0.05"
d.OQ
13.0 11.3
12.6
18.5
14@O
13.9
15.4 13.4
9.80
13.1
74.1
66.1
75.7
65.2
79.2
60.3
70.1
50.4
46.5
57.8
53.8
58.7
48.2
516
228 214
246
223
270
231
235
204
196
229 215
210
159
202
03/08/99,03/17t99.03r-4,L9A9C 0
RSD SUL Day.
DADL 4@ML
0 4% 16%
<I-
<LOQ 21.9 3.05 18.1 2.37
10.4 7.29
9.59 5.04
9.42 19.8
11.9 24.1
ETS-8-5 0 ExcelVersion7 0
WM223.xls
3r26199
FACR TOX-030 Covance# 6329-2n
Study: productNumber(Test Substar=): matrix:
MethodiRevision: AnalyticaElquipment System Number
inumnwnt SoftwareNcrtion: Fdcnam: R-Squamd Value: Slope: Y-intacept: Date of ExuactioWA=Iyst: Dateof AnlyadAtWyst: Dow of Dun Reduction/Analyst:
26 Week CaPuic ToxicityStudy with PFOS T-6295(PFOS) Monkey Sera Ers-B-4.oand ETS-9-5.0
inCY--IPS
SoupO2DI" MauLynx 3.1 See Attachments
See AnacbnicoU
See AnnOments See Attachments
3/IS199JCP/SAN 03/16199DRB 03/17/99DRS
Umple Data
M-kcYs
WEEK
20 MONKEY Group DM
SERA
Sample
Method Blk Method Blk MatrixDik
H20 Bik-1
H20 Bik-2 RabbitSam Bik-1
MatrixBlk
RabbitSen Bik-2
MatrixBlk MatrixBlk QC -250 ppb
QC-250ppb
Monkey Sma Blk-1 Monkey Son Blk-2 RBS03159-MS-1 RDS03159@-MSD-1 RRM3159-MS-2 RES03159-MSD-2
Group 1
105508M
control 0.0mgftfty
05517M 105519M W552DM 105526M
105527M
105529F 1053"F
10553 1F
ED5535F iossw 105549F
Group 2 Low-Dose 0.02nigwday
105514M 105515M 105516M 105S21M
105537F
105541F 105547F
10555OF
Group 3 tAd-Dow 0.5mVWday
105505M 105510M 1055ism 105523M
105524M 105528M 105532F
105539F
105539F
105345F
1055"F
IOSSS2F
Group 4 High-Dose 2.0m&&S/dAY
W55mm 105507M 105309M 10551IM 105512M
105522M
105533F
1055.*44F
105536F 10554OF
105542F
105551F
LiffuocfQuantitatio(nLOQ): 25 n$/mL
Method DetectionUrnit4MDL): 12 ngtffil
PFOS - PofluorooctanesulfOnme
PFOS Can&
PFOS Reported
UshaL
Avmge PFOS ug/mL
0.00
40L
0.00
chOL
@G%ML
0.00
40L
0.00
<Mt)L
4d@ML
0.00
NA
O.W
NA
NA
232
94%
209
84%
ns
191 77%
1"
79%
78%
30.4
0.0434
31.4
0.0523
19.3
4.OQ
232
4.OQ
30A
0.0461
31.5
0.0788
51.6
0.0770
38.3
O.CY722
28.3
0.0615
19.4
<LOQ
29.6
0.0630
39.1
O.CY711
U.6
15.5
86.7
13.3
101
14.9
99.3
-17.6
70.2
13.8
73.2
%.6
17.9
359
51.3
237
77.8
253
79.0
249
78.9
0.0551
0.06" 15.3 15.3 24.3
319
93.8
220
79.9
223
67.6
79.5
255
86.4
201
59.1
210
97.6
171
57.0
204
81.5
154
533
72.5
381
197
292
173
315
in
352
159
309
179
302
181
179
393
210
30.3
.178
274
192
441
241
302
189
3.10
165
194
DateEniemwB y: oimm. 03/17199LAC
Date VerifiedBty: 0 Calculatedwithoutsample 105550F.
RSD StL D*V.
<ML
NA !is 2%.
29.4 0.0162
9.5 O.OD65
11.4 1.74 14.6 2.23 74.4 18.1
10.5 8.37-
25.4 18.4
7.62 13.7
14.0 27.2
Ers-9-5.0 Excel Version7.0
sera223.xis
3r-6199
FACT TOX-M Covance# 6329-2n
Studr. ProductNuniber(TestSubuance): Metric mcdmdntevision: A#WyticalEquipnwg System Number.
lmmrom Softwamtvemon: Rim=: R-SquaW Value: Slope: Y-interew: Data OfExwacdWAsslyst'. Dam ofAnalytWAaWyst' Dateof Data Reducdov]AnalYst:
26 Week CapsuleToxicityStudywithPFOS T-6295 (PFOS) Monkey Sem ETS-8-4.0and ErS-9-5.0 Arnelia062498 A Soup 0201 MassLynx 3.1 See At=hmau See Atmcbmms See Atmcbnvm See Artachmimu 3/16/99RWW/SAH 03/17199,03123t99DRS 03/23i99.03/24t'D9R9B
inCYnOftwiPm McxkcYs
Sample Data
WEEK 24 MONKEY
Group Dow
SERA
Semple
PFOS Cmc.
PFOS Reported UK/ML
Averap PFOS
UWML
RSD SUL Dev.
blediodBlk Medwd Rik MatrixBik MatrixBlk MatrixBlk MatrixDik QC -250 ppb
QC -250 ppb
QC -230 ppb
H20 Sik-1
om
H20 Bik-2
O.W
RabbitSaa Blk-I
O.W
Rabbit Sent Blk-2
O.DD
k6i*-" Sem Blk-I
O.W
Monkey Sem Elk-2
O.W
RDS03169-MS-1
219
RBS03169-MSD-1
236
RDS03169-MS-2
231
RBS03169-MISD-2
213
RBS03169-MS-3
224
RBS03169-MSD-3
220
<t4DL <MDL <bwL d4DL
NA NA us "S 93% 86%
9D% 0%
<MDL <MDL
NA 92% 19% 90%
-<MDL <t4DL
NA 8% 8% 2%
Group I control 0.0mg4may
1055MM M5517M 105519M lO552DtA
105526M 105527M
105529F
10553OF 105531F
10535F
M5544F 105549F
Group 2 Low-Dow 0.02mViWday
W5514M
mssism 105516M 105521M 105537F
105541F 105547F
10555OF
Group 3 Nfid-Dose 0.5mgwday
iOSS05M 105510M M5519M 105523M [05324M
10552$M 105532F 10553&F
105539F
105545F
W55"F
105552F
Group 4 ffir,b-Dose 2.0mgwday
105306M 105507M 105509M 105511m
IOSS12M
[05522M
105533F
1055'44F 105536F
10554OF
105342F
1
IOSSSIF
Urnitof Quantitatio(nLOQ):25 as/mL Medwd DetectionLirWi(MDL): 12 nghnl
36.4
OAM
30.3
OAW
25.9
0.0517
19.4
d.OQ
24.8
4.OQ
36.7
0.0765
40.4
0.06
24.2
<LJDQ
25.9
0.05
<LDQ
16.2
<LA)Q
33.4
0.06
115
18.3
95
13.6
132
17.6
141
n.9
134
16.7
133
71
83
108
15.2
251
96.6
196
91.4
191
73.4
164
74.4
201
86.8
iss
80.9
145
78.7
153
60.6
142
96.8
168
79.3
196
70.6
139
77.1
297
247.1
234
234.4
m
m
258
257.9
309
308.6
233
291.4
290
237.6
225
250.1
2(17
210.8
219
197.8
240
226.5
131
iv.1
Due Entemd/By: 03/24/99LAC
Dam Vairm& By: 0
0.0612 0.05"
18.12 16.25 82*26 75.41 267.86 216.64
17.7 0.0109
15.4 D.OD94
21 3mBl5
0.942
10 8.38
12 9.04
11.61 31.1
12.0 26.0
PFOS . panuorooctanesulfanaie
ETS-9-5.0 ExcelVersion7.0
sem223.xls
FACR TOX430 Covance# 6329-223
Study: ProLluLNtumber(TestSubsmcc): Matrix:
MethodfRevision: AWytical Equipment System Number. lnsuvfnewSoftwamfvcrsion: Fikaum: R-Squamd Value: Slope: Y-Inumew: Date ofExuactioWA=Iyst: Daw ofArAlysislAaWyst: Doe ofData Reduction/Aoalyst:
26 Week CWIUJG ToxicityStudy with PFOS T-6295 (PPOS) Monkey Sera ErS4-4.0 uW ET"-S.O Amelia 062499, Soup 020199
inCYnOffhOISUSMOn"
M"sLynx 3.1 See Attachments
See At=hmem See Atuwhnmu See Auacbffmu 3/ifit99RWW/SAH 03117)99,03r2i/9D9RB/MEE 03n3M, 03n2J99 DRR/MEE
Sample Data
WEEK 26 MONKEY
SERA
Group
S-Ple
Dan
Method Blk
Method Bik MatrixRlk MatrixBlkMam Blk MatrixBlk QC -250 ppb
H20 Bik-I H20 Bik-2 PAwt Sem Rlk-1 RabbitSem Bik-2 Monkey Sam Bik-I --monkey Sem Bik-2 RBS03169-MS-1 RBS03169-MSD-1
QC -250 ppb QC -250 ppb
RBS03169-MS-2 -RDW3169-MSD-2
RDS03169.MS-3 RBS03169-MSD-3
Grmg 1 Control
M550am W5517M
0.0mgA41&y
M5519M 105520M 105526M
105527M 10529F
10553OF
105531F -
105535F 1055"F
105549F
Group 2 Low-Dose 0.02mpwday
105514M 105515M 105516M 105521M
105537F 105541F
105547F
10555OF
Group 3 Mid-Dow 0.5mgwday
105505M IDSSIOM 105518M 105523M
105524M 105529M
105532F
105538F 105539F
105345F
105548F 105552F
Group4 High-Dwe 2.0mWWday
105506M 105307M 105309M lossiim 1053 12m
105522M
105533F
1055.UF
105536F
105.WF 10ii42F
105531F
Limitof Quanuution(LOQ): 25 nVmL
Method Dc=tion Urrit(MDLI: 12nghnl PFOS . Perfluorowtanesulfanate
PFOS com aghnl
O.OD O.OD 0.00 0.00 O.W 0.00 210 232 229 193 211 213 30.9 31.0 27.1 16.1 26.7 41.7 41.22 59.68 30.12 16.61 21.61 34.69
ist 143 131 112 101 113 140 85 255 149 142 176 160 156
209 13D 216 269 145 142 m 213 m 248 295 298 340 log 155 279 206 163
DateEnwmdtBy: DateVerirae&By:
PFOS Rq-riad
ulftl dAm 4d6mL -CMDL 4d6WL CMDL <tmL as% 94% 92% 78% 95% 0% 0.0619 0.0673 0.0521
4DQ
0.0"9 0.0935 0.0765 0.0918 0.0538
<LOQ 4.OQ
0.0694 20.2 20.6 17.2 21.1 16.8 18.5 16.2 14.2 116 162 86.7 89.9 86.7 77.8 79.2 73.6 g(r.1 ICY7 $0.6 69.4
m 227 m 177 iss 'P76 253 233 193 21-P1 206 177
63/-2319L9AC 0
Art PFOS agiml <MDL d@ML 40L 89% 95% 96%
0.0623
0.0729 19.8 16.4
103
93.3-
216
214
RSD gt& Dew.
<TADL <MDLcMDL
10% 17% is
22.9 0.0143
21.7 0.0158
9.91 1.76
10.9 1.77
30.4 31.4
16.3 13.5
21.0 45.5
13.0 27.7 M Moribund
ETS-9-5 0 ExcelVersion7.0
scra223.xls
3r-6199