Document Qq47EKMOw2GRBjR21Kx4Lwyv

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooetanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FX-3103. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFX-3103, 3%. Currentinformatioinndicatesitisa mixtureof0.96% PFOS, 20% diethyleneglycolbutylether,50.56% water,15% ethyleneglycol,4.32% sodium octylsulfate6,.0% polyoxyethylenemonooctylphenylether,2.5% propane sultonefoamer,0.5% sodium nitrate0,.06% sodium laurylsulfate, and 0.1% benzotdazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Date may not accurateiy reffectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute static GLP: No Year study performed: 1985 Species: Selenastnimcapricomutum Source: Not given. Element basis:AJgalcellcounts(cells/mL). Exposure period:96 hours StatisticaMlethods: IC5ovaluescalculateudsingprobitanalysisor other regressionmodels. Analyticalmonitoring: Not given. Test Conditions: Algal NutrientMedium: Not given. Stock and testsolutionpreparation: Not given. Exposure vessels: Not given. Agitation: Not given. Number of replicates:2 Initiaclellloading: 2.05 x 104 cells/mL Number of concentrations: fiveplusa blankcontrol Lighting: Not given. Water Chemistry: Not given. RESULTS Nominal concentrations: Blank control,100, 180, 320, 560, and 1000 mg/L 96-HourAlgalGrowthResponse NominalConc. (mg/L) Control 100 180 320 560 1000 Mean Coll-Count (cells/mL) 1,790,000 1,770,000 1,080,000 720,000 310,000 200,000 PercentInhibition -1 40 60 83 89 Element values: 96-hour IC50= 236 (193 - 273) mg/L Element valuesare based on nominalconcentrations. Reversibilitoyf Growth Inhibition:Not given. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstanceexhibitsa 96-hourlCw of236 mg/L witha 95% confidenceintervaolf 193 to273 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Study lacksdescriptioannd recordsofthe method followed.There isno characterizatiofnthe testsubstance purity, and lacksanalyticaclonfirmationoftheamount offluorochemicalproportionin the solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-1,1985. OTHER Last changed: 6/26/00. ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST ALGAL CELL COUNT, no./mi HEMOCYTOMETER METHOD* TEST DAY-0@@-, 14ATERIAL TESTED: ALGAL SPECIES'! 6reen Alaa TEST TYPE 51]anastCum capricarnutum LR DATE TREATMENT 100 7-- ?1 TOTAL OF 25 FIELDS COUNTED ISO ilia 1C) no./ml 1,000 )COC) @,@o IL)OO MEAN + SD 1) @qc)kooo eo) 000 (Lftoo,coo 1)1001000 1 0(00)000 ) 000 C)80 OO(D 15z-j zo Ilzo(ooc) I'2l-010cc) 6,ZO 000'. 500,000 61 C) 000 Xzo 00() @,oot DOE) 2-Dot()00 0.1 m deep Hemocytometer;area of field 1 x I and 0.1 m deep. ENVIRONMENTAL LABORATORY TABLE 1. SUP@mjkRY OF ACUTE TOXICITY or WVIIA ,-@7As TO FRESHWATER ALGAE (SELENASTRUM . CAPR ICORNUTUM)(1) Chemical concentration Control Inn Percent Inhibition in Standing Crop (2) 48-Hr. 96-Hr. Based on Based on Based on Cell-Count Cell-Count Cell Dry Wt. 40 loon (3) ic so (95% Confidence Limits) mg/l l(pI Accel2tibility of Test The Selenantrum capricornutum following quality criteria:, control cultures have met the - Exhibited log phase growth within . - Attained a 96-hr. otanding calle/ml) crop of6M&& hours max. 48 hr.) ( celle/ml (min,. 105 lo,-@d,N 7-0,.500 (1) Data were averages of two replications for each test concentration. (2) Based on cell-count (no./ml) and/or cell-dry weight (mg/l)* (3) Ic values were calculated using Peobit Analysis or other reg5?ession models of experimental data.