Document QkrEG6EvDBMyYd8GxbBeMnGe7

/dR226- ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, orFC-109-X. (Glycine, N-ethyl-N[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS #299151-7) Remarks: The test sample is FC-128. Current information indicates it is a mixture of 86% test substance and 14% C4-C7fluoroalkyl carboxylate compounds. 3M production lot number 751. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1977 Species: Pimephales promelas Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Probit analysis. Test fish age: Not noted. Length and weight: Average length = Not noted Average weight = 1.1 g Pretreatment: Not noted. Test conditions: Dilution water: Aerated carbon-filtered well water Dilution water chemistry: Initial Dissolved Oxygen: >9.0 mg/L hardness: Notnoted alkalinity: Notnoted pH: Notnoted Conductivity: Not noted Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Not noted. Number of replicates: one Number offish per replicate: 10 Number of concentrations: five plus a negative control 000338 Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 5.7 - >9.0 mg/L (control exposure) 5.1 - >9.0 mg/L (180 mg/L exposure)* pH range (24 - 96 hours): 7.6 - 7.8 (control exposure) 7.5 - 7.9 (180 mg/L exposure)* * 180 mg/L test group data given because total mortality occurred in the 320 mg/L test group by 72 hours and all data was not collected. Test temperature (0 - 96 hours): 62 to 67F RESULTS Nominal concentrations: Bk control, 32, 56, 100, 180, and 320 mg/L Element value: 96-hour LC50 = 100 (70 - 142) mg/L All element values based on nominal concentrations Statistical Evaluation of Mortality: Probit analysis was used to calculate LC50 value and the corresponding confidence limits. CONCLUSIONS The test sample 96-hour LC50 for fathead minnow was determined to be 100 mg/L with a 95% confidence interval of 70 - 142 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. This study lacks documentation and information on the methodology. Sample purity was not properly characterized. Test concentrations were not characterized. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, completed on August 26, 1977. OTHER Last changed: 5/18/00 000339 740101 ENVIRONMENTAL ENGINEERING. LABORATORY AQUATIC TOXICITY WORK SHEET L2L Type Test 96 Hours - S T A T J C . Test Organism Fathead Minnow_______ Date Started_____ 7 Material Tested pC. -- LO T * 7%~/ Avg. Weight Avg. U _____ Size_______________ ~r Date Completed -11 Time Started Q\OQ Pk Plotted LC50- Dilution Water Carbon Filtered - Well Water ('AtV'ftV'CCl) 24 hrs % 48 hrs D.O 72 hrs D.O % 96 hrs D.O % D.O Control / o o /, 1,1 b 0 0 1$ /CO 7 8 6-2 0 0 7.$ I S lo o 0 0 . lb srt 1 `1 a .\ /0 0 7 7 7 7 0 0 3 1 0 0 IC O 7,9 3 4 0 0 7S 7 2 0 0 1go j0 0 ' , -ti13 0 0 77 I S 70 320 7 0 7,f T'/r 3 0 7 2 I S ' 0 Average Temperature C> Comments: lUinfc- O .O . > . C W / ^ " TEMP, "p J ' LCgQ Calculation lOOC^TOT^tf- cs 5.8 10 0 7b s n 6 . 0 103 1rs 5.A 6,2- ' } 0 ( 3 S s 6.3 s o i s 3 o 6 'e0 10 is C! ! -1 6x0 0 . 'T7 _ (. % Survival 00034/5 100 <11? / 0Q 03& AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET Date Started: Time Started: (AM)(PM) Test Organism (Fathead minnow) Type Test: (Continuous Flow)(Static) Avg. Wt. (gram) Avg. Size (inc Exposure Period: -Hour Date Completed : Diluent:(carbon-filt.-St.P. City Wtr.) Analysis by: 0) K No. of Doses Plotted Cone. Observed % Mortality (2) Expected % Mortality (3) 0-E Corrected . ObservedValues Expected Contributi to chi(fro Nomograph 10 2 lo 3 1 CD <3 T 304 \ % 0 5 "'2 C' O O ) c0 6 7 K= 0 Animals/Dose Total contribution to chi Total Animals = .2 chi (P-.05) for (K-2) (chi2)= contrib. to chi x Tot. Animals degree of freedom (from Table 2) is less than ; therefore, the data are not significan heterogenous. 95% Confidence Limits for LC^q * LC 84 LC 50 fee Slope function (S) = LCR84.'/LC50 + lc50/l c 16 LC16 // 2 4 = '/ i f +/-41 2 Total number of animals used between 16% and 84% expected effects (N'') JQ ac50 = (s),2.77/^j' _ = (] ) = (from Nomograph #2) \.d'? LCgo/fLCso = lower limit = \so / \ = _ 7 i jq X fLC^Q = upper limit = ten:. X 1,42. * M i 2 . LC 50 (95% confidence limits to Z 4 -? lo - / (1) Do not list more than two consecutive 0% effects or more than two consecutive 100% effects. (2) Expected value for any dose should be greater than 0.01% or less than 99.99%. (3) Corrected value for each 0% or 100% effect (from Table 1). * There is a 95% chance that the true value of LC^^ lies within these limits. 000342