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/0 Submitted to: BIOSEARCH, INC. p.o.box 8598 ph;iadelphiap,ennsylvania19101 telephone: (215) 848-4499 3M Company 3M Center St. Paul, Minnesota 55101 Material: Sample Received: Date of Report: Test:. Object of Test: Method of Test: Results: 3M Company - T-1395 December 6, 1975 March 4, 1976 Primary Eye IrritationStudy - Rabbits To determine the degree of irritation,if any, which the subject material may produce when instilledinto the eyes of albino rabbits. This test was performed in order to classify this material under the Federal Hazardous Substances Labeling Act. The methods employed in the testing, evaluation and in the grading of the test material are those described in Section 1500.42 - Hazardous Substances and Articles, Administration and Enforcement Regulations, Federal Register, Vol. 38, No. 187, P. 27019, 27 September 1973. The sample was used as supplied. Six healthy young adult albino rabbits were used in this study. 0.1 gm of the experimentalmaterial was instilledinto the right eyes of the test animals while the other eyes remained untreated to serve as controls. The test material was not washed from the eyes. The treated eyes were examined at 1,24,48 & 72 hrs. 5 & 7 days followinginstillationof the test material into the eyes. Interpretationof the results was made in accordance with the grading system outlined in the "IllustratedGuide for Grading Eye Irritation By Hazardous Substances". The results of the tests are shown in the accompanying Table which covers each of the animals treated with the test material. 3M Company T-1395, Primary Eye Irritation Study. Conclusion: Based on the accompanying table, the subject material is a primary ocular irritant within the definition of the Act-Reference: Section 1500.42(b) (1) (2) P. 27019 and requires cautionary labeling with respect to that section. Karl L. Gabriel, V.M.D., Ph.D. Director TABLE I Grades for Ocular Lesions Material: 3M Company - T-1395, as supplied. Rabbit Number 1 Co I c Hours After Application 24 Co T- c 48 Co T- c 1 0 5 10 0 2 0 5 10 0 3 0 5 6 0 4 0 5 10 0 5 0 5 10 0 6 0 5 8 0 Co - Cornea 1 - Iris C = Conjunctivae 5 6 5 4 54 56 5 6 58 0 5 6 0 5 2 0 5 2 0 5 4 0 5 4 0 5 2 5 Days Co I- c 0 5 4 0 0 0 0 0 0 0 5 2 0 0 0 0 0 0 72 Co I 0 5 0 5 0 0 0 5 0 0 0 5 7 Days Co I 0 5 0 0 0 0 0 5 0 0 0 0