Document Qk3qXOgmek21VeXRNOpgbr895

DEPARTMENT o f t h e n a v y N A V A t- r e s e a r c h l a b o r a t o r y W A S H I N G T O N , D . C . 2 0 3 7 3 --9 0 0 0 IN R E P L Y R E F E R TO: 3900 Ser 6180-752 K " ! S S S S S ? X i i T i U ' E S -- * Laboratory Sea Systems Command (SEA 5122P) Sub j ; FEOXATMI NCGOOMIPASNMYN G ^ G E N T S TMMA^N?UiFcACTTOURREEnD LBiQYUITDHE CCONHCUEBIB NAATTIEONFAILRE Ref: Enel; (a) Chubb National Foam Ltr, dated 11 June 1991 (1) ^ cPieB of subject report copy to: (w/copy of e n d n n . NAVSEASYSCQM (Code 56Y5^ ililln) Nava! Weapons Support C ^ t e ^ o d e 304E) Distribution Authorized to US Government Agencies and their Contractors Only; All other requests shall be forwarded to: Commanding Officer Naval Research Laboratory, Wash. DC. THIS INFORMATION HAS NOT BEEN APPROVED FOR PUBLIC RELEASE. US00000349 6180-752A:CRF 30 October 1991 Subj: RESULTS OF QUALIFICATION OF AFFF LIQUID CONCENTRATE FIRE EXTINGUISHING AGENTS MANUFACTURED BY CHUBB NATIONAL FOAM COMPANY (a ) Military Specification, "Fire Extinguishing Agent, Aqueous Film-Forming Foam (AFFF) Liquid Concentrate, for Fresh and Sea Water," MIL-F-24385E(SH), November 1990 (b) Qualified Products List, "Products Qualified under Military Specification MIL-F-24385, Fire Extinguishing Agent, Aqueous Film-Forming Foam (AFFF) Liquid Concentrate, for Fresh and Sea Water," QPL-24385 1. _ AFFF concentrates manufactured by the Chubb National Foam Company and designated as Aer-o-water, 3EM (3% type 3) and Aer-o-water, 6EM (6% type 6) were tested in accordance with the procedures outlined in reference (a) and AFFF agents listed in reference (b). 2. The test data obtained are summarized in the attached tables. 3. Both candidate AFFF concentrates met the requirements of reference (a). Carl R. Fulper Fire Protection Section Naval Technology Center for Safety & Survivability NRL Code 6184 Head, Fire Protection Section Naval Technology Center for Safety & Survivability NRL Code 6184 Attachments 1) Tables 1-12 2) "Results of 96-hour, Fundulus heteroclitus Flow-Through Acute Toxicity Tests on an Aqueous Film Forming Foam (AFFF) Sample from the Naval Research Laboratory," EA Engineering, Science and Technology," October 1991. 3) "Final Report: The Acute Toxicity of Aer-o-water 6EM to Mummichog (Fundulus Heteroclitus), May 1991. Enel (1) to NRL ltr 3900 Ser 6180-752 Distribution Authorized to US Government Agencies and their Contractors Only; All other requests shall be forwarded to: Commanding Officer Naval Research Laboratory, Wash. DC. THIS INFORMATION HAS NOT BEEN APPROVED FOR PUBLIC RELEASE. US00000350 Table 1. - Q a a s teristic Mflusi.rarf Refractive Index, minimum Viscosity (centistokes) maximum @ ffC minimum @ 2s3c Hydrogen Ion Concentration (pH) Spreading Coefficient, minimum Foamabiiity, minimum ?rainFresh Water; Expansion Ratio SSeeaa Water; ExpansTiiomneR(matiinoutes) 25% Drain Time (minutes) Rlm Formation and Sealability Fresh Water; Half Strength Full Strength Sea Waer: Half Strength Full Strength Quintuple Strength Corrosion General; maximum -Steel (milli in./yr) -Cu-Ni (milli in./yr) -Ni-Cu (milli in./yr) I ,, V ' B r o n z e (milligrams) Localized CRES -Steel Tota! Halides, maximum (ppm) Dry Chemical Compatibility Burnback Resistance, minimum (s) Environmental Impact Specifications Requirements lype 3 Type fi 1.3630 1.3580 20.0 2.0 7.0-8.5 3.0 120..00 7.0-8.5 3.0 6.0 6.0 2.5 2.5 6.0 6.0 2.5 2.5 N Sustained Ignition (for all solutions) No Sustained Ignition (for all solutions) 1.5 1.0 1.0 100 No pits 500 1.5 1.0 1.0 100 No pits 250 360 360 Results Type 3 Type6 -MM_ 6EM 1.3697 1.3587 6.2 3.3 3.3 2.0 8.1 8.5 8.1 8.5 8.8 9.1 4.4 4.4 8.1 7.7 3.6 3.8 No ignition No ignition No ignition No ignition Flashed-NS No ignition No ignition No ignition No ignition Flashed-NS <<<<11110...0500 No pits 328 <1.5 < 1.0 < 1.0 <100 No pits 174 396 361 I X'city' LCso. minimum (mg/l) COD, maximum (mq/i) b ^ o/COD, minimum 51,00000,000 0.65 1000 500,000 0.65 ( 1) (2) T e " r s % uEr f r ; L Scr a >500i1) 476,778 0.92 >1000(2) 283,231 0.94 te d a 5% mortality rate was observed' 1000 ppm (10 mg/L) of 3% AFFF concentrate NS N o % S : gn Sied in,he MIL SPEC- wuld be above the 1000 ,ha`theLQo value US00000351 Table 2. Stability Test Results (Paragraph 3.3,2) Property Measured Specification Requirement (all solutions) Results Type 3 Type 6 Fresh Sea Fresh Sea Spreading Coefficient, minimum Concentrates (fresh water only) 3 q 6.6 6.9 Foamability, minimum Concentrates; Full Strength (fresh and sea) Expansion Ratio 25% Drain Time (minutes) Pre-mixed Solutions; Full Strength (fresh/sea) Expansion Ratio 25% Drain Time (minutes) g.O 2.5 ' 6.0 2.5 8.8 7.6 9.0 7.9 4.1 3.2 4.4 3.4 8.6 7.4 10.0 8.0 4.4 3.3 4.3 3.6 Film Formation and Sealability Concentrates; Half Strength Full Strength Pre-mixed Solutions; Full Strength No Sustained Ignition No Sustained Ignition No Sustained Ignition NI NI NI NI NI NI NI F-NS NI NI NI NI Fire Performance, 2.6 m2 (28 ft2) Concentrates; Half Strength (fresh/sea) -Extinguishment Time, maximum (s) -Burnback Time, minimum (s) Concentrates; Full Strength (fresh/sea) -Extinguishment Time, maximum (s) -Burnback Time, minimum (s) Pre-mixed Solutions; Full Strength (fresh/sea) -Extinguishment Time, maximum (s) -Burnback Time, minimum (s) 45 300 30 360 30 360 42 43 40 44 (1) (2) 644 (3) 30 30 30 29 513 398 501 444 29 30 28 30 418 373 475 365 Stratification Concentrates Pre-mixed Solutions; Full Strength None None None None None None None None Precipitation, maximum (% volume) Concentrates Pre-mixed Solutions; Full Strength <0.05 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05 (1) (2) (3) Nl F-NS Burnback pan removed at 300 sec; burnback fire self-extinguished at 615 sec. Burnback pan removed at 289 sec; burnback fire self-extinguished at 338 sec. Burnback pan No Ignition removed at 202 sec; ' burnback fire self-extinguished at 250 sec. Flashed, Not Sustained 2 US00000352 Table 3. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of 3M FC203CE and CNF 3EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Reqirement (all solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 9.0 9.0 4.3 4.1 Nl Nl 9.6 8.1 4.0 3.9 Nl F-NS 29 25 473 432 None <0.05 28 447 None <0.05 27 394 None <0.05 F-NS Flashed, Not Sustained Nl No Ignition 3 US00000353 Table 4. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of 3M FC-203CF and CNF 3EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Bumback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement fall solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 8.3 8.0 4.6 4.1 NI Nl 9.1 8.6 4.1 3.5 Nl F-NS 29 27 544 389 None <0.05 28 462 None <0.05 28 374 None <0.05 F-NS Flashed, Not Sustained Nl No Ignition 4 US00000354 Table 5. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of Ansul AFC-5A and CNF 3EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Bumback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement fall solutions} 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutic Fresh Sea Fresh Sea 8.7 7.9 4.3 4.0 Nl Nl 9.0 7.3 4.0 4.1 Nl Nl 24 27 567 444 None <0.05 24 27 485 435 None None <0.05 <0.05 Nl No Ignition 5 US00000355 Table 6. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of 3M FC-206CE and CNF 6EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Seaiability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement la ll solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 8.5 8.5 4.4 4.4 Nl Nl 8.8 7.8 4.5 3.9 Nl Nl 27 28 547 459 None <0.05 29 28 624 506 None None <0.05 <0.05 Nl No Ignition 6 US00000356 Table 7. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of 3M FC-206CF and CNF 6EM ProDertv Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement fall solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 8.8 8.5 4.0 3.3 NI Nl 9.5 9.0 4.2 3.6 Nl Nl 30 29 481 393 None <0.05 27 30 505 444 None None <0.05 <0.05 Nl No Ignition 7 US00000357 Table 8. Interagent Compatibility Test Results (Paragraph 3.3.3) for 50/50 Mixture of Ansul AFC-5 and CNF 6EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealabiiity Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement (all solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 8.3 7.1 4.4 3.9 Nl Nl 8.6 7.9 4.4 4.1 Nl Nl 29 28 479 450 None <0.05 25 27 544 433 None None <0.05 <0.05 Nl No Ignition 8 US00000358 Table 9. Interagent Compatibility Test Results (Paragraph 3.3.3) for one-quarter/one-quarter/one-quarter/one-quarter Mixture of Ansul AFC-5A, 3M FC-203CE, 3M FC-203CF and CNF 3EM Property Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement (all solutions) 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Nl No Ignition Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 9.5 8.5 3.8 4.1 Nl Nl 9.0 7.5 3.8 4.3 Nl Nl 29 28 476 397 None <0.05 27 28 488 461 None None <0.05 <0.05 9 US00000359 Table 10. Interagent Compatibility Test Results (Paragraph 3.3.3) for one-quarter/one-quarter/one-quarter/one-quarter Mixture of Ansul AFC-5, 3M FC-206CE, 3M FC-206CF and CNF 6EM ProDertv Measured Foamability, minimum Expansion Ratio 25% Drain Time (minutes) Film Formation and Sealability Fire Performance, 2.6 m2 (28.0 ft2) Extinguishment Time, maximum (s) Burnback Time, minimum (s) Stratification Precipitation, maximum (% volume) Specification Requirement (all solutions^ 6.0 2.5 No Sustained Ignition 30 360 None <0.05 Results Concentrates Pre-mixed Solutions Fresh Sea Fresh Sea 8.3 8.3 4.0 4.2 Nl Nl 9.1 6.7 4.5 4.3 Nl Nl 27 25 436 412 None <0.05 22 25 479 548 None None <0.05 <0.05 Nl No Ignition 10 US00000360 Table 11. Fire Performance Test Results (Paragraph 3.4) Property Measured 2.6 m2 (28.0 ft2) Fire Tests Extinguishment Time, maximum (s) Fresh water, Half Strength Full Strength Sea water, Half Strength Full Strength Quintuple Strength Burnback Time, minimum (s) Fresh water, Half Srength Full Strength Sea water, Half Strength Full Strength Quintuple Strength 4.6 rrr2 (50 ft2) Fire Tests Extinguishment Time, maximum (s) Burnback Time, minimum (s) 40 s Summation, minimum Specification Requirement (all solutions) 45 30 45 30 55 300 360 300 360 200 50 360 320 Results Type 3 Type 6 3EM 6EM 37 39 29 24 38 41 30 27 31 32 452 (1) 431 468 413 439 425 406 304 330 38 36 371 407 325 320 0) Bumback pan removed at 248 sec; burnback fire self-extinguished at 311 sec. Table 12. Cap Removed Torque Test Results (Paragraph 5.1.1.1.1) Pass/Fail Criteria Maximum Torque (In-lb) 50 Type 3 3EM 37 Results Type 6 6EM 44 11 US00000361 .-FONOANAVAMANKLAA"RHQEFUSFEE}AOSRUACSMHF-P'ILLLEAMBFOFRORORAMMT'OTINRHGYB. Otobfr W91 E. Project 7OW3J0 MM H US00000362  4 Engineering, Science, and Technology, Ine. EA Project 70003.10 RFELSOUFOWNLONTA-ATSVAMHAONRLF(OAAARUQFEF9UGS6FEHE-)HOASAOURACSUCMURHFPTILFELLEuAMTnBFOdOFRuXORlOuIARCMsTMIhOTTeIYRNtHeYGrEToEclSitTus Prepared for: NavaWl RaessheianrgcthonL, aDb.oCr.atory Prepared by: EA EEnAginMeiedSr-piAnag1tr5l.kaSsLn,cotiMivecneaRtcroeeyn,glaaiConnniddracl2Tle1Oe1c5ph2enroaltoiognys, Inc. October 1991 Date Date Report Number 881 US00000363 1. INTRODUCTION At the request of the Naval Research Laboratory, Washington, D.C., EA Engineering, Science, and Technology, Inc, performed a flow-through acute toxicity test on a sample of aqueous film-forming foam (AFFF), fire fighting agent. The product tested was AER-O-FOAM XL-3 which is manufactured by Chubb National Foam, Inc. The objective of these tests was to determine the 96-hour median lethal concentration (LC50) of the test material to Fundulus heteroclitus (mummichogs) using lethality as the endpoint. US00000364 2 . METHODS AND MATERIALS 2 .1 SAMPLE DESCRIPTION The test sample was delivered to EA by courier. Two liter bottles each containing Chubb National Foam's AER-0--FOAM XL--3 aqueous film--forming foam (AFFF) were shipped to EA, logged in, visually inspected against the Chain of Custody Records, and assigned an EA accession number (EA sample number (ATI-776). The foam concentrate sample was stored at room temperature (20C) when not in use. 2 .2 TEST ORGANISMS Juvenile mummichogs, Fundulus heteroclitus, constituted the test organisms. The test organisms were 30-35 days old at test initiation. The fish were obtained from cultures maintained at Multi Aquaculture Systems, Inc., Amagansett, New York. The lot of F. heteroclitus (Lot Number FU-005) was received on 18 September 1991. The fish were fed flaked food daily until used in testing. The mean acclimation temperature and salinity for Lot FU-005 were 20.1 0.5 and 22.2 0.7 ppt. There was no mortality observed in this lot of fish during the acclimation period. The mean length and weight of Lot FU-005 organisms were 18.18 mm (1.57) and 0.12 g (0.03) respectively. 2 .3 DILUTION WATER The water used for acclimation, holding, and for the flow-through studies was 20 ppt artificial seawater. Dry synthetic sea salts (Forty Fathoms, Marine Enterprises, Towson, Maryland) were added to dechlorinated tap water and allowed to age prior to use. A summary of the chemical characteristics of Forty Fathoms is presented in Table 1. The source of the makeup water was Baltimore municipal water supply, which upon entry 2 US00000365 into the laboratory was passed through a high capacity activated carbon dechlorination unit which removed chlorine and hexane soluble compounds. 2.4 TOXICITY TEST OPERATIONS AND PERFORMANCE At 1500 on 25 September 1991, a 48-hour static acute rangefinding test was initiated to provide information for the selection of test concentrations in the flow-through test. The concentration series employed in the rangefinding test was 100, 300, 1,000, 3,000, 10,000 ppm and a Forty Fathoms dilution water control. There was one replicate container per test concentration, and five organisms were placed in each chamber. The 96-hour flow through study was initiated 1600 on 27 September. Test procedures used in the flow-through study followed those described in the protocol ATS-FAF-GT-01 (Attachment I) which is contained in EA's Aquatic Toxicology Laboratory Quality Control and Standard Operating Procedures Manual (EA, 1986). EA's protocol is in conformance with guidelines contained in ASTM's E729 dynamic procedure, and with EPA (Peltier and Weber, 1985) and Standard Methods (APHA, 1989) documents. The test employed Masterflex peristaltic pumps which continuously delivered test solution from the stock solution tanks to splitter assemblies for each test concentration. The fill and dump splitter assemblies divided the solution flow equally to the two replicate chambers for each test concentration. The test was comprised of five concentrations and a control. The AER-0-F0AM XL-3 AFFF test series was 135, 240, 420, 750 and 1350 ppm (vol/vol) and control. Daily, a fresh 60 liter batch of each test concentration was prepared in a 20 gallon aquarium using 20 ppt Forty Fathoms artificial seawater as dilution water and control. There were a total of six 20 gallon aquaria, one for each test concentration and control. The Masterflex pumps were calibrated to deliver test solution at 40 ml/min to each concentration's splitter assembly. Masterflex silicone tubing was used, and test 3 US00000366 solution did not come in contact with any pump parts. The test chambers were 19 L all glass aquaria, which were calibrated to hold 14 L of test solution at the standpipe overflow. Each test concentration exposure group consisted of two replicate chambers with 10 organisms each. Mortality observations, pump delivery calibrations and water quality determinations were made daily throughout the 96-hour exposure period, and were recorded on the data sheets. Copies of the original data sheets are included in Attachment II. Temperature, dissolved oxygen, pH and salinity were measured in each batch of new test solution daily and for the old test solutions in at least one replicate of each concentration at 24, 48, 72, and 96 hours. Aeration was provided to all of the test chambers when the dissolved oxygen-saturation in the high test concentration dropped below 40 percent. The aeration rate was 100 bubbles/minute per EPA guidance. Using the total number of dead organisms per replicate and concentration at the end of the exposure period, the 96-hour median lethal concentration (LC50 value) was calculated by the binomial, moving average, and probit analysis methods as described as Stephan (1977). Copies of the statistical analysis are included in Attachment III. 2.5 REFERENCE TOXICANT TESTS The reference toxicant used was sodium dodecyl sulfate (SDS). The test species was exposed to the appropriate graded concentration series to determine if the lots of organism were within the acceptable limits of EA's Aquatic Toxicology Laboratory. The test duration was 24 hours and treatments were not replicated. 2.5 ARCHIVES - Original data sheets, memoranda, notes, and computer printouts are archived at EA's laboratory facilities in Sparks, Maryland. Primary data and associated information are retained for a period of 5 years unless a longer period of time is requested by the Naval Research Laboratory. 4 US00000367 3. RESULTS In the rangefinding test initiated on 25 September, all organisms exposed to 3,000 and 10,000 ppm AER-0-F0AM died vithin 24 hours. At 1,000 ppm there was 20 percent survival after 48 hours, and at concentrations of 300 ppm and below there was 100 percent survival at 48 hours. The 48-hour LC50 for the rangefinding test was 682 ppm. Aeration was provided to all test chambers 17.8 hours after the start of the 48 hour rangefinding test when the DO in the 1000 ppm chamber was measured at 3.8 ppm (40 percent saturation). A summary of the water quality measurements recorded during the 96-hour flow-through study is presented in Table 2. On Day 1 of the study, aeration was provided to all head tanks and exposure chambers due to depressed dissolved oxygen levels in the test chambers. The results of the acute toxicity test are presented in Table 3. At the end of 96 hours of exposure, there was 100 percent survival in the 135, 240, 420 ppm AER-0-F0AM and the control treatment groups. In the 750 ppm treatment group there was 5 percent survival after 96 hours. All organisms exposed to 1,350 ppm AER-0-F0AM died within 24 hours. The resulting 96-hour LC50 for this test was 583 ppm with a 95 percent confidence interval of 420-750 ppm. The LC50 for the reference toxicant test conducted on F. heteroclitus lot FU-005 was 13.1 mg/L sodium dodecyl sulfate (SDS). This test result was within EA's acceptability range of 4.3 - 18.6 mg/L SDS for heteroclitus reference toxicant tests. 5 US00000368 TABLE 1 SUMMARY OF WATER QUALITY CHARACTERISTICS ASSOCIATED WITH FORTY FATHOMS MARINE MIX HYDRATED TO A DENSITY OF 1 025 USING DISTILLED WATER Parameter Aluminum Antimony Arsenic Barium -Bicarbonate Beryllium Boron Bromide Cadmium Calcium Carbonate Cerium Chromium Chloride Cobalt Copper Fluorine Gallium Germanium Iodine Iron Lithium Magnesium Manganese Mercury Molybdenum Nickel Nitrogen Phosphorus Potassium Silicon Sodium Strontium Sulfur (SO.) Thallium 4 Tin Titanium Tungsten Vanadium Zinc Molecular Weight 12271..70 1111552675621534647611736445926839520009942017.................9...93.8.769950400009408039 121256488910223351905073867438918405................4999714610107096 -, Forty Fathoms (PPm) 01.000070..4.0100.21065 00.04.0016.10000220000....7009012 010.80.0,0061.0200.97520 0.0001000.00.02000000.1.000...,1000,..000.00000,604.000042320..200.0.0.000080000020809..0409446705404595078430354 US00000369 TABLE 2. SUMMARY OF WATER QUALITY RANGES FOR 96-HOUR FLOW-THROUGH ________ 'ACUTE TOXICITY TESTS FOR THE NAVAL RESEARCH LABORATORY Test Number EA Accession Number Temperature (C) M. DO (mg/L) Salinity - <PPO FT-09-27-91-786 ATI-776 17.3-20.7(a) 7.2-8.2 <1.0-7.5(b) 18.2-20.3 Temperature measured on Day 1 and 2 old test solutions was below the 20+2 C target temperature. It is EA's opinion that the below-range temperatures did not affect the test results. (b) A DO value of 3.4 mg/L (40 percent saturation) was measured in the 1350 ppm concentration on Day 1. All test vessels were aerated at this time. US00000370 TABLE 3 RESULTS OF THE 96-HOUR FLOW-THROUGH ACUTE TOXICITY TESTS USING Fundulus heteroclitus Test number: Sample Description: Accession Number: LC50 value (a). FT-09-27-91-786 AER-O-FOAM XL-3 ATI-776 583 ppm (420-750)^D ' Exposure Concentration (ppm)-- Percent Survival Control-- 100 135-- 100 240-- 100 420-- 100 750-- 5 1350-- 0 Ta) LC50 value was calculated using the binomial method. (b) Values in parentheses represent 95 percent confidence intervals. US00000371 ^ ,'^ 1 ^ iMMMHiHaBj Sfr; . -i. " i.r ;" ; t # rip*f : 3 ; : ; / '> i: .1 ;;.:w;r- i : :> ;i -;^:Or.:y'!.;r':,:';*:.>:.yy:/-:y-.:"f,-'.:;.~.'.;yvy;'->^ i"-.: -iA-tA't-C f=; .v.fV::=A : f ^ V ..-.^A.-:A.'i'i.;_' S - :^ - c - fx y .}.m ^^ ":*-^ f-w .:...: :-: r m ^ : : :cH-;::,/'e;vy'.';>V'-T.Vv:" -..- f ^ :tf-;*' ? * C ' - k f ". ' - - i Vr-.(T?rS. :.:."3 >V-:::'" J r . ' . ^ - V . *>Y\Yy*:\ f n f ' f V ' Ti?V :} W f A - ' /C > - '.,-v y .H y ;'..!-f v - ' :.. - '..Y' * . y*.*-,:y'Iy^ aW y. s :Y.:;=j / ^ . p h ' - A A " ja:-. ' " : : '- ; : : : v , \ : a : : ; : >..-1*r*A A \ - \ . , . : .- .'>.v -ru*-.-.-y; *. : y y y / y y u . : , y ; :;-?&& ..-*-/*;. r> , rj . y y :-.s r v i ^ - v v 1; tA :C )p ; < a %'. y y ^ S S V ' y y . : *'; US00000372 PROTOCOL ATS-FAF-GT-Ol 24. FLOW-THROUGH ACUTE 96-HOUR LC50 ASSAY WITH A FISH 1. TEST OBJECTIVE To assess the toxicity of a test article to a fish species under flow through conditions and determine the 96-hour LC50 using lethality as the test end point. 2. TEST ARTICLE 2.1 Description/Identification Unless otherwise specified, the test article will be supplied by the client. Adequate chemical specifications with special reference to hazardous properties and storage conditions also will be supplied by the client. 2.2 Methods of Synthesis Information on the methods of synthesis, stability, and data on composi tion or other characteristics which define the test article are on file with the client. 3. EXPERIMENTAL DESIGN 3.1 Test Organisms A fish will constitute the test species as defined by project needs. 3.2 Source Stocks are laboratory reared, obtained from a scientific organism vendor or field collected using techniques that will be specified in the report 3.3 Holding Conditions Upon receipt, fish are transferred to holding tanks which are continu ously replenished with dechlorinated municipal tap water, synthetic sea water , or other appropriate dilution water. The fish are acclimated to laboratory conditions for a minimum of 7 days ..prior to testing. The laboratory is maintained on a 16-hour light, 8-hour dark photoperiod cycle by a programmable electrical timer. All fish stocks are examined regularly throughout the work week. Dead fish, or those displaying abnormal swimming behavior, discoloration, or pronounced lethargy are removed as observed, and recorded on appropriate log sheets. Fish ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 1 of 8 PROTOCOL ATS-FAF-GT-Ol stocks are fed a commercial fish food a minimum of once daily throughout the work week. They are not fed for 48 hours prior to initiating a test. 3.4 Dilution Water The source of dilution water is the City of Baltimore Municipal Water System. Upon entry to the laboratory, the water is passed through a high-capacity, activated-carbon filtration system to remove chlorine, detergents, and hexane-soluble organic materials. This water source has proven safe for aquatic organism toxicity testing, as evidenced by main tenance of the multigeneration Daphnia cultures, with no evident loss of fecundity. If requested, reconstituted fresh water, synthetic sea water, or other dilution water may be used. 3.5 Test Concentration Series The test concentration series to be used for definitive toxicity tests will be selected following preliminary tests to bracket appropriate concentration ranges. A minimum of five concentrations and diluent water controls will be used. If it proves necessary to use a carrier solvent to disperse the test article in aqueous solution, an additional set of solvent controls will be used. All test concentrations and controls will normally be conducted with two replicates. Additional replicates may be used at the client's request. 3.6 Toxicant Delivery System A solenoid-activated proportional diluter system similar to that des cribed by Peltier and Weber (1985) will constitute the toxicant delivery system. The proportional diluter will be calibrated prior to test initi ation and at test termination. Each test concentration should be at least 50 percent of the next highest concentration in the series. Test vessel calibrations, cycle times, flow rates, and other pertinent data will be reported. 3.7 Test Vessels and Test Volume Test ^vessels will be of sufficient volume as to not exceed the organism loading ratio and minimum number of daily tank volume replacements. 3.8 Test Organism Number , A minimum of 10 test organisms will be used in each test replicate, con stituting a minimum number of 20 fish per treatment group. Fish will be randomly assigned to each test replicate. ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 2 of 8 PROTOCOL ATS-FAF-GT-Ol 3.9 Test Environment The test vessels will be maintained at a temperature similar to that at which the fish have been acclimated, but within the recommended test range for that species. Test temperature will not vary more than +2 C from specified acclimation temperature. The tests will be conducted on a 16-hour light, 8-hour dark photoperiod cycle. 310 Analysis of Test Concentrations for Test Article If required, test solution replicates may be analyzed for chemical analytical verification of achieved test concentrations. The analytical method and number of determinations will be formulated after consultation with the client. Where chemical analyses are necessary, both nominal and actual measured test solution concentrations will be reported. 3.11 Test Observations Test organisms will be observed at a minimum of 24, 48, 72, and 96 hours after test initiation to determine number of animals killed by test article concentrations. The study will terminate after completion of the 4-day observation period. The study may be extended, however, at the request of the client. Routine water quality measurements are performed on the test solutions and diluent water controls according to the following schedule: tem perature is measured daily from all test chambers; dissolved oxygen, salinity (when applicable), and pH are measured initially from all test chambers as well as at test termination, and from the control, low, medium, and high concentration at 24-, 48-, and 72-hour intervals. Specific conductance, total alkalinity, and total hardness are measured from the diluent water and sample (when applicable). Specific conduc tance may also be used for concentration monitoring at the request of the client. Test solutions will not be aerated unless dissolved oxygen concentrations fall below 40 percent of saturation. Analytical methods will be conducted according to APHA et al. (1985) and EPA (1979). Dead fish are removed when observed. At the end of the 96-hour test period, a minimum of 30 surviving fish are removed from the test con tainers , placed in labeled specimen bags, and frozen. The fish are subsequently thawed, measured to the nearest mm (standard length), patted dry, and weighed to the nearest 0.01 g. The average length and weight of the test fish are reported, along with their respective ranges and stan dard deviations. ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 3 of 8 PROTOCOL ATS-FAF-GT-Ol 3.12 Data Analysis The LC50 values and associated statistics will be derived by the probit analysis, moving average, and binomial test methods as described by Stephan (1977). Depending on the nature of the data, other methods may be used including the probit approximation method of Litchfield and Wilcoxon (1949), SAS probit analysis (SAS Institute 1979), or graph ical interpolation using the log concentration vs. percent lethality as described by APHA et al. (1985). The methods used will be specified in the report. 4 . FINAL REPORT The final report will be prepared to contain the following information: Objectives and procedures stated in the approved protocol, including any changes made to the original protocol Identity of the test articles by name or code number and their strength (i.e., quality/purity) and a description of any pretreatment . Source of the dilution water, its chemical characteristics, and a description of any pretreatment Test concentration series used and duration of the assay Man standard lengths and weights of test fish, the respec tive ranges, and approximate loadings of fish to test volume (g/L) Routine water quality characteristics of dilution water and selected test concentrations . Any unforeseen circumstances that may have affected the quality or integrity of the study Signature of the project coordinator, program manager, and quality control officer as authorization of the report. Location of all archived data and the original copy of the final report at EA Items of data to be included in the report consist of: experimental design and test performance; effects on general appearance of test organisms; morbidity and mortality; tabular presentation and appropriate ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 4 of 8 PROTOCOL ATS-FAF-GT-Ol statistical evaluation of water quality characteristics; and survival data where necessary. 5. QUALITY ASSURANCE 51 Amendments to Protocol Amendments to the authorized protocol instigated by EA or by the client will be made only after proper authorization. Such authorization is achieved by completion of the Amendment to Protocol form by EA and the client. 5.2 Standard Operating Procedures Unless otherwise specified, all procedures mentioned in the protocol are subject to detailed Standard Operating Procedures (SOPs) which are con tained in the SOP manuals of the participating departments. These SOPs and protocols follow the U.S. EPA's Good Laboratory Practice Standards (GLPs) (EPA 1983). 5.3 Reference Toxicant A reference toxicant bioassay, utilizing sodium dodecylsulfate (SDS) or other appropriate reference toxicant, will be used as an internal quality check of the sensitivity of each batch of test organisms. Testing will be prior to or concurrent with the test or following the acclimation period for newly acquired organisms. The results of each test will be compared with historical toxicological information of reference toxicant tests performed at EA on the species, to determine if the results are within acceptable limits using the control charts outlined in Peltier and Weber (1985). 5-4 Quality Assurance Evaluation The study described in this protocol may be subject to internal audit by the Quality Assurance Unit. A quality control officer will be respon sible for monitoring each study to assure the client that the facilities, equipment, personnel, methods, practices, records, and controls are in conformance with EA's QC program and EPA's GLPs. 5 -5 Inspection by Regulatory Authorities In the event of an inspection of EA by an outside authority during the course of the study, the client will be consulted before inspectors are permitted access to any of the project records or the experimental areas. ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 5 of 8 PROTOCOL ATS-FAF-GT-Ol 5.6 Archives Ccpies of project-specific records shall be transferred to the client promptly after the project is completed or as negotiated and budgeted with each client. Original primary data will be retained by EA for 5 years. Primary data include laboratory data sheets, records, memo randa, notes, photographs, microfilm, and computer printouts that are a result of the original observations and activities of the study and which are necessary for the reconstruction and evaluation of the study report. 5.7 Location This study will be conducted by the Aquatic Toxicology Services Department of the Scientific Operations Division of EA Engineering, Science, and Technology, Inc. at the Mid-Atlantic Regional Offices, Sparks, Maryland. 6. SPECIFICATIONS FOR FLOW-THROUGH ACUTE TOXICITY TEST WITH FISH 6.1 Basic References American Public Health Association et al. 1985. Standard Methods for Examination of Water and Wastewater, 16th edition. APHA, Washington, D.C. 1,268 pp. American Society for Testing and Materials (ASTM). 1980. Standard Practice for Conducting Acute Tests with Fishes, Macroinvertebrates, and Amphibians. ASTM Designation: E729-80, Philadelphia, Pa. pp. 285-309. EA Manual T-101. 1981. Aquatic Toxicology Studies, Quality Control and Standard Operating Procedures Manual. Revised, August 1986. (Internal document prepared by Aquatic Toxicology Services, EA Engineering, Science, and Technology, Inc.) Peltier, W.H. and C.I. Weber. 1985. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms. EPA-600/ 4-85-013. U.S. Environmental Protection Agency, Cincinnati, Ohio. U.S. EPA. 1983. Toxic Substances Control; Good Laboratory Practice Standards, Title 40 CFR Part 792. Fed. Regist. 28(230):53922-53944. ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 6 of 8 PROTOCOL ATS-FAF-GT-Ol 6.2 Test Specifications Test organism: Fish age: Fish length: Temperature: Light quality: Light intensity: Photoperiod: Diluter and test chamber flow rate: Aeration: Loading ratio: Dilution water: Test container: No. of concentrations: Test animals per container: A fish species to be specified in final report All fish for any one test will be selected from the same age-class The largest fish will not be more than 50 percent longer than the smallest fish To be specified Wide-spectrum fluorescent lights 50-100 f.c. 16-hour light, 8-hour dark Continuous flow-- a minimum of five volume replacements per test chamber during a 24-hour period None, unless dissolved oxygen falls below 60 percent saturation in first 48 hours of the test or below 40 per cent saturation thereafter In general, test chambers may not contain greater than 0.8 g/L biomass Dechlorinated tap water, reconstituted fresh water, synthetic seawater, or appropriate receiving water 19- or 38-L all glass aquaria Minimum of five concentrations and a control in duplicate series 10 (if loading is exceeded, more replicates or larger aquaria may be used) ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 7 of 8 US00000379 Feeding regime: Test type and duration: End point : PROTOCOL ATS-FAF-GT-Ol Animals are not fed during the test unless fish are too small to survive 96 hours without feeding. Feeding is terminated 48 hours before test initiation in the lot from which organisms are obtained Rangefinding-- 24-96 hours Definitive-- 96 hours Reference toxicant-- 24 hours Lethality-- defined as cessation of opercular movement and inability to respond to external stimuli (gentle prodding with glass rod) ETC-T-101 EA Engineering, Science, and Technology, Inc. Effective May 1981 Revision 3, August 1986 Page 8 of 8 US00000380 US00000381 AQUATIC ORGANISMS ACUTE TOXICITY TEST DATA SHEET Priecl No" P TESTfStetfe Flow-through, Renewal A.Test Article: Test C o n t a i n e r : b a t f l Client: AavvIa I--MS.d f -- Test Volume: j$P 0 tnjf Q C Test No.: ~7?3 Test Duration:.... Sanpte No.:....... t f f l r f l f r Oilution No.: 20% o .S a lt H-^O TEST ORGANISM: Beginning Oate.^/p ^ fa l Time: / STTP Common Name: /W u n .^ u f l Ending Date: wilAf Timm-_ /r52>cJ Scientific Name: h rte/o d i-ftiS, Lot No.: OO^i A lkalinity- Sample IDiluent Source:_____ M fl Hardnels:" Acclimation:2^jprAge 3 0 days Conductivity:, Test Con Con. tainer or% No. jg fa a l ltaimber of Live Organisms Tempiratur (C) 0 24 48 72 98 0 124 48 72 96 L > rf.1 fl.%% ALL TESTS SALT OR BRACKISH WATER TESTS Dissolved Oxygen (mgffiter) 0 24 48 72 98 1,3 ____ L____ Salinity (ppt) 0 24 48 72 96 0 24 48 7? M 7* * `l /w 08 0 ?4 4R 7? 9Q .'y ' i \ m m /f.o tl ?i /& * 5 5 fi.0 im g 1 1 Id} m m ? D ifS IS |M 7-y 7 ,0 >.2 /? > /M ID,ODD 0-- m ii- 1 M ?4 Instrument Number Correction Factjr Time Investigator |9 AS $ 13 13 n m A)fl rvpf IS AS * 4 *7 21 n %f\ m 1(0* Lb AS 13 93 13 K -y fo '/*> vyo I5to- AS AS 1I 3 .O 04) .c m s *11 l-S AS US00000382 AQUATIC TOXICOLOGY BENCH SHEET SET UP (SMALL VOLUMES) Project No.: Client QC Test No.: ' lOOO'b./o (ZoifarfX Date/Time qfazfa 1 iS ZS i ^2^hf \SUd \&&j Activity . Dilutions Made Test Vessels Filled / Transferred Organisms Head Counts Investigator LS KrC Sample No.: Dilution No.: fi~r h 71 k 2.0 7,* lI+ M7iO l %OO p p t A . 3+ock Test Concentration C o rrh fb l / 0 0 pp* 30 p pM . I0DQ ppiv Volume Test Material (ml) 6.0M>fp* s4oct) Final Volume S rvJl \S So" / ,0 V fp* ISO 500 X/ US00000383 Project No.: Client: QC Test No.: AQUATIC TOXICOLOGY BENCH SHEET l o o p % >o $iSc/.\rck R P -o q a ^ i- 7P3 ~~ Date/Time Activity ______________Investigator c l u ^ o o ^ rep. - 7-1 n o o igsesa. 3,000 rsp - 7 .3 , ccrv jjpw. r^pt - 7 . o |AI\ iOtooo m A S,Oro fepS <axe d e ^ c?50 t> -0 . e-f 1 , 0 0 0 pp<*. r?p - 3 . * ? f" 9'/.') (jh 0 ^ 5 0 n P^/rvx.yM^^ x\c d r 3 o M S OifrA-tild ((>5S TO>-0. Jr (OOOp^v Ctp - V- uS US00000384 AQUATIC ORGANISMS ACUTE TOXICITY TEST DATA SHEET Project No.: 1-9QLLE1SL TEST: Stalic^ F i^ S r ^ Q j ^ l TEST ORGANISM: Tesi Article: ^ T e s t Container: Common Name: Beginning Date: U J jjjT im e - fb t^ Ending Date: l o f i N l Time- /fc o n g ? - .. T ^ t V o l u m e : --------------------------------------------- OC Test No.: f E J f t - j ? - " - Test Duration: *(,hr f Sample No.: T >-7 7 6 Dilution No.: ZO 9/t> Qll 11,0 Scientific Name: Lot No.: F 14 - oo tf' Source:_____ M I L Acclimation: 1 or Age J O ' la ^ T Alkalinity: _ Hardness: days Conductivity:. Diluent ALL TESTS SALT OR BRACKISH WATER TFSTS Test Cnn- Con. tainer Nuimber of LfvaOmanisms Tempiaratur (C) Dissolved Oxygen (mg/llter) PH Salinity (ppt) or % No. 0 24 4a 72 96 0 24 48 72 96 0 24 48 72 96 0 24 48 72 96 0 24 48 7? 96 A t_ f f |0 /O IVI 1 I H 70- i . f M 7-3 7-5 V1 ?-o F w .l tf.y 10.0 ZL_ (> 10 io lo 0 ?4 48 72 0ft 13sr /I H |0 IO )0 IK d i M . *>.5 l b )0 IO )0 l'f L i M 7,1 9,i ?-l 111 7 11-7 10.c o?^0 rt JL / \u>.< 0 6 io |0 lo ,0 in iO.fr l i 7-1 7-3 7.1 tf.i l I l f |1 m VJO A 1-1 IO 1 IO lo iv i M HA jD.fe X L p /O fO-' lo -Z 2 L A r-1 (0 a 1 1 i n m I1W )M /O 1 0 -- -- 1M I X H i 73 HI M n io h i l *,(? Dii ? S i ih f io. i l f I p r t- i 10.1 /5ST> A 6 i 0 -- | -- -- --m ------ -- EL --- io __- ilf Instrument Number Correction Fact>r 1* i i qs Nt m Time investigator 'tiS I f f a 5 im IS10 US"? m i M va 1 L i tS J-S iP I e* lP TL % % .0 M if<-; M i 1011 oo<5 -S(' - H i uS f i 1 7 9S lji 77* l? f i'y l o'$ d# \0U LS t-S 11 t \ \ 0 0,0 0 c 1545" /'I 07 10C7 tS IP % US00000385 Project No.: A L t Test Article: f . ri> fi <^l ft*. Client: PT - D'h 1 17 I^ -7 3 < * 1OC lest No.: ....( . T i T io__________ T E S T : Static/fT6w:thr Test Container: ^ Test V o lu m e : /'/ Test Duration: 1 6 Xao Sample No.: 4 T) ' 7 TO-_________ _ Dilution No.: _ J f 0 * - S i 4 W t r neytal TEST ORGANISM: Beginning Date: J " `3 7 1 f Ttme: Com m on Name: c Scientific Name: f . A i~ fc r< P c Y / Lot No.: F H 'OOS' Ending Date: / b j I j 6 1 T im e : f ( 0 & 7 ) ' Sample Diluent Alkalinity: __________ _________________ _ Source: Acclimation:. _m X_ Hardness: _______________ ______ .or Age. 3 > days Conductivity: 74Cootn%e. n1r6-.s.*t ConNo. Numberof Live Organisms 0 24 48 72 96 All Tests Temiaeratare(C) Dissolved Oxygen (mg/liter 0 Us_ 48 72 96 0 24 48 72 96 0 24 pH 48 72 Salt or Brackish Water Tests Salinity (pptj 96 0 24 48 7? 96 *a`--" n 4R 72 96 A f6k-ki _4i_ M17.3 / 7.S- 8* ?, r.7- / fid. 17-K _____ A -7 !-- ----Ai>---- A 1 A L io __ 7/ ft / --/-i L1 ,2 A > A76t> _ _ > __ 1 7 ---- 1 A A L A 11-4 .I / 7-7 'M life 177 7d.6 / 11 *8 7*3 --A Iir 6 t 1.1 f.i 7-8 7? ?.o 7- 7 7 f I76 II,7 I1.5 M 30.fl lie. H-1 L iTl no.to 5 * 6`2- f1 7.1 76 75 7-5 fr-- 111 30.o r?-t 30.1 OA 7,? 7-1 Ilio 3M m JOi _ / r? y-5 77 1-H 7 117 3o.n fH 1-7 / 1Dl 2lftJ?lf / !i& *1-0 fjp 7 i 74 7-2 -j.'b 7 I l i ao.| 21.7 % f fl-7 ;V>T> .J _ V A I7.< / 3.1 (HoJ? 7 7# f hI ~ 111 7 --/ / Instrument Numoer Correction Facto r Time Investigators ^,, / , r --l 4y - 1/ 1L <?3 $ 3 13 JL MA __ L LL41 3 ,,at p. X 2 AiA $ 1 n P m 6 / cg (f ll/ tf)0O w I 7 / 1 -? 13 13 1 3 f II11 r7 e J7'"? r % 0 . .O 6 O.o t-- / osti 0^0 0f5*> f53 0 5 57 I5to 1^ AS t s t s .5 .s is l w- 7 5 A-S 6 $ tlS L-b 6^7 US00000386 DREDGED MATERIAL SOLID PHASE BIOASSAY FLOW RATES SHEET Project No,: 7CV7> 5 , 1 0 ________________ Client: M:WU ________________ QC Test No.: r r - >3 -:n*7/-m>_________ Initiated: Date ______ Time: l( to0 Target Flow Rate: . ____________ ml/min t. U Treatment (-cvbi>l Repair Number Q ate^ i hi Date `I tflr Dal 4 3? FLOWRATES(mi/min) 3. 4 Dal 1|SoRI Dele s 3S 5 Date 6 Data 7 Date 1 35 .3 40 ijo 73^ /3>t> . . _ i _____ Xf 4 X 4 & h 3* - 4 .. . L-y 3 ^ It'* Hi Si ^ Ji -------- ^ -- \o^ l h H -- - 51 ~> i o % 5%-->Ho Ho n ~ ^ Y ir HH .3 5 --> ^ 5 w tnit Time ------------------------- ii-- J ^ T J^^XXo US00000387 AQUATIC TOXICOLOGY LABORATORY BENCH SHEET Project No.: 7 0 0 0 3 , / Q Client:____ I intese Test No.: p r -0 e! 2 7 1 ( TEST S E T -U P T E S T INITIATION T j e s t c o n c e n t r a t io n s f p i f s Date/Time Initials Activity (.%or MaterialTest Cone. mq/O Volume T e s t (m l) Final vo lu m e <!/*>/*( /^3o Tff Dilutions made lo COL `linfa I I'-wr Test Vessels Filled f3 i Lf 0 HZO ii ! i*'I3 V ,J .> 7 a : boG ^ S H Transferred Organisms .'s Head Counts 7TO i 3S6 IS" g I h Comments: INTERMEDIATE DILUTION PREPARATION AND FEEDING US00000388 AQUATIC TOXICOLOGY BENCH SHEET Project N o.: 7 0 0 0 3 / p C lie n t: ~A/w*. f Q C T e s t N o.: FT Date/Time Activity Investigator iI 1'7 '3*3 w| l -^Ak )r% i'd-O a /-I '+' A C\\*L- a t a -. I f "TuOo A 5>W 7 5 D ?V^ b -A.-ter ..<I'KV. - iAre JUA<i. 7 j ,r>k t 'J t4 a t e l\ 1 a bi>,, . U.`i , M1\ <_Aj,\(.-< 6 kH T)-0. A t)-u . aorv 7 2) p o m Tan t -- t 7 7(7 14 l a n k . - -- ), O i^efakurti kw a t t M - e M Tanbs a T$t T o a k i tSedt JfcfO , b-0.^ : I2>5 ppm. W O ppm, */>0 ppm "75 ppiv lest Wn(c 6? 5 S-b i(ssy.) Mtcwi T a n k 7 -) 6 -9 67 2- * ( > >0 6 .e ^hl^i 'fen 4\rks- b KViXikintrtzoUonS -Pcle^iAiSl(aitt,+Ufho-Kj.s1"la^kA.sr<zCtZcYl LS i2o Mt t US00000389 Length-Weight Data Sheet v ^ v 'r ic Project Nolsi.: 7 0 0C3. '0 QC Test No(s).:_ Test So*:.f' hf+|TO d S Lot No ; 1nvBstiqator: T im e . Date: o us )7 / 0 Individual No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 28 27 28 29 30 Range **-- SLO. Weight (g) o.ol 0' \*D 0. 19 o. 10 0 .O ? 0 . >-h 0. 12- 010 D. 1 3 0-M D. 0 1 O-J 0 O - / ' * t o 0.12. o. 10 0 . 0 e! 0.0! O ' II . O'Cfi n . /o o. r 39. / 3 0. 0 ? . /V 0 .11 O./V O.IY <9- l (a 0. \ 6 `0 . 0 1 -- O-l^ 0. ) % ... O ' O ' b Standard Length (mml )U.%t al . 6 3 19.24 If.7 ? 1?. 1 1 ! aa 19.97 2.0.0% V /b-5ib \1*lH f S '. l 1Y-2.0 n.m /6.?4 Ka-IH |?.00 17-17 Ua-1%. If,'/? )f. 7 9 iS->? nr. ? 7 /9-7/ H . / 14, 3< 19. / S 19*21 .... 1 *1 ? _________ L i n ______ > Ch US00000390 Length-Weight Data Sheet P ro je c t NolsL: Q C T e s t No(s).: T5t .. L o t No.: i o cc.s . >c N -^ fD ri ! n u iis fig a rn r: ...... Time, D a te : ...... . ^ rV J / --^ 1 ~J/ C (srr^Ch Individual No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 W eigh t (g) Range S .O . 0-o l Q. I 5 ~ 0. Ie ! 0- ID o.c? I'b 0. i l OH 0 0. IS D- H 0. 0 7 O-i D 0 . 10 O' l o 0 -IX 0- 10 0. 0.0! O . /f 0 -0 7 o. /o 0. /X - 9. I S o. on 0 -0 ? 3 , / o. n o- i i o-iy 0- 1& 0 -- o-i^ o. 0-os ' Q t `4 '/3 Standard Length (mm) 0 - nj t 1 1 7 -3 2 1. 9 ? 17 1 ^ }( OH 1?. 7 ? I?- Il 1? I X 19. ^ 7 20-03 lp- S? \l,n H /5 V 7 6 1?. 3 o n -n n 16.?? Ho-~7H nf.co n -ii Ha- 7 X )? 7 9 i - ? is"-? 7 li-H I I e)- L I e), 2> 19- ! 3 I e), H S . f a V - Sii. *.|? USI US00000391 I B B B B |B f iB B P B I||B |^ B H B B B B B p B H B B M B B p B B llB j|H . W/HlBlImKIBBIlIIBllBmIlltIBSmKmammllliIHKIBIBBSOEBSSSBi jBjMjl3iliEfpl^^i a ^ MliWIMiiliiB!il i H M a f f li^ ^ M J iilM lM B I ttS a a B B B W B H H M H B IS M ^M .'YfcV#^:;1 -/.'Y Y ;Y<vP"VD,i:vc P \:.:; P Y P y &*>*' 'YP P V -,'rf ;r''V V p V - p P P Y p p ,..;mm': : ^ : M >. ;^v:::-, . , - . . . . :.:-;^ 1 ::><?' ir f l'-iJ ' > . :: ''" ! ; ...O ' *,." P ' ':\ \ '* -v.-- ' *:/ " 'Va.::. ' >\ '.-. . ' *; '-v ^ .:.: .# ."* 7 J .* '.-V t .. % ' * :.. :"'.;> :V ..-,: ": a lfllm :'.:v;,v.:*:-.-;?.vt-'-i:..\s.v-V".:. -- ^ -- JM BiiiiBafflMMeMaMiiaiiiBiMiiM :wy*?V".:";^ S ' m .'.''#{e ".'"-"v"y y s k y .... ;;;:npi;:v:^*Hj*r5Y-,;[..Y ' ^ W * > X p'V't::>'-. ....a/ , " . ;*:: VTMV >F- tf:'.& -- m m S.:.'"i{..v'...V.r.iM.:1-Hi.-."B"*,:v-'- US00000392 [190, 192 ]STEFZZ.BAC 13-0ct-91 11:25 AM TEST NAME: NAVAL RESEARCH RANGEFIND-783 ^ie^icir^if^ieificieieirificic^ic'k'kic'k'kit'k'k'kiieicTk'kicif'k'kiririKicicic'kie'kie'k'k'k'k'kic'k'kic'k'kit'k'k'k'kirif'k'kic'k'k'k'kic CONC. NUMBER NUMBER PERCENT BINOMIAL 10000 EXPOSED 5 DEAD 5 DEAD 100 PROB.(PERCENT) 3.125 3000 5 5 100 3.125 1000 300 100 5 5 5 4 80 18.75 00 3.125 00 3.125 AT A CONFIDENCE LEVEL OF 95 PERCENT THE BINOMIAL TEST SHOWS THAT THE LC50 IS BELOW 3000 SHOWS THAT THE LC50 IS ABOVE 300 AN APPROXIMATE LC50 OF 682.424 IS OBTAINED BY NONLINEAR INTERPOLATION BETWEEN 300 AND 1000 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE METHOD NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE REASONABLE. ******************************************************** US00000393 [190,192]STEFZZ.BAC 13-0ct-91 11:28 AM TEST NAME: NAVAL RESEARCH F-T 786 ************************************************************************ CONC. 1350 750 420 240 135 NUMBER EXPOSED 20 20 20 20 20 NUMBER DEAD 20 19 0 0 0 PERCENT DEAD 100 95 0 0 0 BINOMIAL PROB.(PERCENT) LESS THAN 0.001 .200272E-02 LESS THAN 0.001 LESS THAN 0.001 LESS THAN 0.001 THE BINOMIAL TEST SHOWS THAT 420 AND 750 CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS BECAUSE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. AN APPROXIMATE LC50 OF 583.039 IS OBTAINED BY NONLINEAR INTERPOLATION BETWEEN 420 AND 750 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE METHOD NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE REASONABLE. ******************************************************** IBiil m/mmIKBKK^iiBItBSmm3SmBm ljjtt MBBMMB "ft-r-,- .... .. .: :.* ,^::;H;^r^::>ia: "-^:^irs?;i=r 3~m r\ * .^::.j:..::j.;:;,-.... ;..;.*...?---T7rrTi.'^.4^C1 "-'t y '' ':;''.W: .'HT'.'.; -gv V.;?"> n 4K^:'/' ":-,**'..':'y-- M ; : 444 :"4J-4 ;.4--4;-.:k'v.t ..;.:::.4-: .. .....iv -.:-`.V-" :Hw:.'f;.:'- ;. ..^^,Y.;..v.::,:....:...,-:_-.-,'!Mi>mi;.i:;ii\-ar:, iiiilillBllliHllliiilllilBIllBiMlliiW^ BBBlliM i 5 f^ T O tv^ C T "..:.. ' *. - s :*s."-,*/..:.**.'l.x.":.*.- m ^ h - :"V : t o .:.: f?-..:.';.r . r "' Ai:v .:,,Y S i:;:-/; :/: ;- r :' *..:**.:..* :!.-..'{*..\ I"v:VV."-'.-b`'..: {';\J"*i+\":.;>:>,:;.,VV:-..,...:.x.. :;j,'.;; !4;YrM4".^V.->,v'.:."vY..-X.,'...'V'`.Y:A'%-""!...: US00000395 REPORT QUALITY ASSURANCE RECORD C L I E N T ^ , )rt Q .r /c : . ^ ir:-k\U i EA REPORT MO. % / PROJECT NUMBER ^ r r f Z./( '________ -- 1 f | >ii / ' A f i i < TYPE ANALYSIS f /f,,., -4(-, -,(,, , y J TEST ORGANISMS f- A A <-< : > 1 REPORT CHECKLIST QA/QC i t e m 1. Samples collected, transported, and received according to study plan requirements. REVIEWER / " / '/ . /(.a*. -Tv;'?-* DATE / c;, 2. Samples prepared and processed accordm g to study plan requirements. // 3. Data collected using calibrated equipment. / // , i y y ? y ^ * Calculations checked: - Hand calculations checked -nA /^ l, / L O J X y / - Documented and verified statistical procedure used. 5. Data input/statistical analyses y . ! / / - / -/^ / y / , complete and correct. .'s~c-c'^7z I k o T C( f 6. Reported results and facts checked against original sources. 7. Data presented in figures and tables correct and in agreement with text. ibOcT^f ' ./ ~ &r-V ^ U z , ( c T c(i 3. Results reviewed for compliance with study plan requirements. / ./ / y y _ ./ ^ Ss* < . -x Si-- 7 j'A' ', -------/ ---------- 'trsL* 7s 9. Commentary reviewed and resolved. AUTHOR : ojL DATE >/ 10* reportas a m o v e d ^ a l i t y assurance/control^requirements have been met and the . - y*-* ` / / / - S j / / / ^ ' /rz/i - P R O J E C T MANAGER " ' ....DATE 111 /6 O c T f / .D A T E " --- - UcZ/f! " ...... DATE----- --- US00000396 AQUA S U R V 6 V , IN C FINAL REPORT THE ACUTE AER-O-WATER 6 TOXICITY OF EM TO MUMMICHOG (FUNDULUS HETEROCLITUS) MAY 7, 1991 BR91-1567A JOB #91-143A3 3oinf Breeze Road Remington. New Jersey 08822 Telephone (201) 788-8700 FAX (201) 788-9165 US00000397 RQUf SURVV, INC Report: The Acute Toxicity of Aer to Funduius hgte.mgll.ttts Sponsor: Chubb National Foam, Inc. Union and Adams Streets West Chester, PA 19382 Testing Facility: A4q9 uapoSiunrtveByr,eeIznec.Road Flemington, NJ 08822 Study ' Number: 91-143A Report # 1567A Date Test Started: May 2, 1991 Date Test Finish: May 6 , 1991 Date Reported: May 7, 1991 Personnel: Y. Terrell C . Lawrence C. Nally '/ft P ^ York Terrell Study Director Date Page 1 of 9 0 / 1 499 Point Breeze Road Flemington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 78 US00000398 f 7 fQUf SUfVV, INC QUALITY ASSURANCE UNIT INSPECTION STATE6 EMMENTTO FMOURMMTIHCEHOAGCUT(EFUTNODXUILCUI8 TYHEOTFERAOECRL-IOT-UW8A)TER, In accordance with the regulations set forth in Good aFRmZ9 2 'v.FedPerraact1icReegifsotrerN,onvcolli.nic4a8,l Laboratory Studies, 40 November 29, 1983, part 7 ' The Phase of study inspection, along with the d a t e s (s) of inspection are shown below: Phase of stgfly Preparation and set-up Exposure period and daily observations Notebook entries Correlation of notebook entries to final report ' - -sanction 5/2/91 5/3-4/91 5/8/91 5/8/91 Comments: ithe t i J !!e o y } i s inspection, all work had been or was Joeing performed i n a manner consistent with the Good Laboratory Practices. Quality Assurance Officer Date Page 2 of 9 499 Point Breeze Road Remington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000399 flQUR SURVV, INC Summary A sample of Aer-O-Water 6 EH was received from Chubb National Foam, Union and Adams Streets, West Chester PA 19382 on F eb ru ary 2 2 , 1991. It was tested for potential lethality to F u n d u lu stthherousgahltHwaayter6 finfish, , 1991. The through conditions, at a heteroclitus on Hay test was conducted under nominal concentration of 1 2, 0f0l0owppm, for 96 hours. Five (5) percent mortality (1/20) was observed at the 24 hour live count, no other mortality was observed during the test. Surviving organisms were observed quiescent (1/19) or surfacing (8/19) beginning between the 24 and the 48 hour observation periods and lasting in some instances through 96 hours. Page 3 of 9 499 Point Breeze Road Flemington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000400 AQUA SURV, INC. II in. Objective The objective toxic effects of of tAheirs-O-stWuadtyerwa6sEMtowhedneteirnmitnhee the acute environment of aquatic vertebrates, under flow-through conditions for 96 hours. Test material Source: Name: CAS N o .: Chubb National Foam Union and Adams Streets West Chester, PA 19382 Aer-O-Water 6EM 112-34-5, 107-21-1 (hazardous ingredients) Batch No.: ACR-29-86 (Exptl) Date of Manufacture: 2/91 Expiration Date: Specific Gravity: Solubility: Appearance: None 1.04 1 0 0 % in water Straw yellow liquid; mild p l Date Received: February 22, 1991 Amount Received: 4 liters Materials and Methods A. Method The method employed was a modification of . ^ e Standard Guide for Conducting Acute Toxicity tests with Fishes, Macro invertebrates and Amphibians E 729-88a. See Appendix A for the protocol. Page 4 of 9 499 Point Breeze Road . Flemlngton. New Jersey 08822 . Telephone (90S) 788-8700 FAX (908) 788-9165 US00000401 i flQUR SURV6V, INC B. Test Organisms Scas igg The test species for this test, being representative of a salt water finfish, was Fyndhlvig heteroclitus. S..lz..e./Age/Phvsical Condition Animals used for this test were field collected and appeared to be in good condition. The mean length of the organisms was 41.6 mm, while the mean weight was 0 . 7 9 4 gm. Source/Acclimation All animals utilized in this test were from Aquatic Research Organisms, New Hampshire. Organisms were acclimated to this laboratory for 14 days. C. Test System is a r s e o f-D J J j& la a .la te g Water utilized for this test was natural seawater obtained from the Manasquan River Inlet, Manasquan, New Jersey. It was filtered through a 0.45 micron filter. _ See Appendix B for water characterization. Temperature The test temperature was 22 +/" 2 C Test vessels The test vessels were 20 (20x25x40 cm), containing li1 0terlitaellrs g la of ss aquariums test solution at a depth of 13 cm. Photoperiod A 16-hour light and 8 -hour dark photoperiod with 15 to 30 minute transition period between light and dark. Page 5 of 9 499 Point Breeze Road Flemington. New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000402 * RQUfi SURV6V, INC Loading Ten into aneiamcahlstewsetrevessseellectceodntaatinirnagndo1m0 and loaded liters of test solution. The loading factor did not exceed 1.0 grams per liter of solution passing through the chamber in a 24 hour period. Test Levels This was a screening test and a concentration of 1000 ppm was chosen. Two replicates of ten organisms were exposed to this concentration. gQntrpl A control was run concurrently using the same dilution water and the same number of organisms as the test concentrations. Beginning the Test The test was conducted under flow-through conditions. The test was initiated by randomly introducing fish acclimated in accordance with the mtiensutt edsesiagfnterint1 o0 the test chambers within 30 liters of the test solution was introduced into the chambers. Proportional dilutors were used to deliver additional test solutions. The dilutors were set to deliver 10,000 ppm 900 ml stock of dilution w solution of Ae ra-tOe-rWattoer1006 ml EM of a (prepared in AS I D I water), mixed immediately before introduction to the test chambers. The flow rate through each test chamber was 6.2 cycles per 24 hours and each chamber was aerated. All test vessels were examined at 24, 48, 72 and 96 hours; the number of dead were recorded and the dead animals were removed and discarded. The fish were observed at the same time and any unusual behavior was noted. Measuring Temperature/PO/bH The temperature of each test vessel was measured and recorded at the beginning of the test and at 24, 48, 72 and 96 hours. Page 6 of 9 C l / i 499 Point Breeze Road Remington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000403 f -- --------- R Q U f SUfVCV, IN C The dissolved oxygen (DO) concentration of each test vessel was m e a s u r e d at the beginning of the test and at 24, 48 72 and 96 hours. The pH of each test vessel was measured at the beginning and at the end of the test. saiia.Ua: The salinity was measured in each test vessel at the beginning and at the end of the tes . IV. Results There was 5% mortality in the 1000 ppm concentration after 96 hours. No mortality was observed in the control group; however, one fish in the c"*j[jdgroup as well as three from the 1000 PP group " " g * ,, through the overflow pipe between the 24 and the 72 hour observation periods (see Table 1) The test solution temperature was maintained at 22 +/- 2 C. (See Table II) Dissolved oxygen was maintained between 5. and 7.8 mg/1. (See Table I I I ) . . m .h lp IV The pH of the test solutions are presented in Table IV. The salinity is presented in Table V. VI Length and weight data are presented m Table VI. V. source of Documentation All original data documentation is being maintained at A4q9u9 a Survey, Inc. point Breeze Road Flemington, NJ 08822 .. h in Chubb National Foam Notebook 003 page 9 through 24. T a b l e .I Mortality Data Peadj oo oo rO O !o c Time Hrs 0.0 c o T ^ e n tr a tio a B looo ppm A 0.0 0A .1.1__ _______ J7LJ7k----------- 0o 00 *0 *0 n *n * * 1 0 ___ **10-____ _ **10 0 * one fish escaped irora ------* * ** Three fish escaped from tank through overflow p p Page 7 of 9 FAX (908) 788-9165 L / j 499 Point Breeze R o o d . Rem ington. New Jersey 08822 . Telephone (908) 788-8700 US00000404 6AQUA SURV V, INC Table II Time 0,0 24 48,,,, 72 96 T e m p g r a t w a i n Degrees C, Concentration u .i_y__ 1000 PP1 AB AB 22 12,. 05 - 21,5 . 22 12,. 05 . 21,5 22.5 21,5 21.5 _22.5 21.5 222222... 005 22.5 21.5 22222222....0055 T a h 1 c TTT Time Hrs o*o 24 48 72 96 . Dissolved oxygen ma/I 0 ..0 Concentration 1 0 0 0 --BJBffl; AB 7.7 6.5 76.. 67 76.. 82 6.7 766... 862 A 76666..... 86108 7B.8 65.. 89 66.. 04 Table iv Time Hrs 0.0 M ______ m ,,0, 0 . A8 . 0 ... 7.9 Concentration B 8.0 7.9 100 0 DDm A8 . 0 B8.1 7.8 7.1 Table V Time Hrs 2Sl Salinity -0*0 AB ? 5 -26. ..21.1...5. o oor"f A .25,0 -EBB B .2 1 1Q 25.t.2 Page 8 of 9 499 Point Breeze Road Flemlngton, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000405 6fQUf SURV V, INC Table VI L s n g t h r W e i g h t Rat a .(ElsiL-ana g r a s s Shrirop Q n l Y U Test Concentration Replicate (% Effluent) Total Length _ (mm) Xs Weight - (9) X s Loading Factor (g/D 30 ORGANISMS WERE RANDOMLY SELECTED AND SACRIFICED TO OBTAIN THE FOLLOWING DATA: MEAN WEIGHT IN GRAMS: 0.794 STANDARD DEVIATION FOR WEIGHT: +/" 0.136 MEAN LENGTH IN MILLIMETERS: 41.6 STANDARD DEVIATION FOR LENGTH: +/" 1.88 LOADING FACTOR IN GRAMS/LITER: 0.794 Length of longest measured test organism (mm): 45 Length of shortest measured test organism (mm): 38 Page 9 of 9 499 Point Breeze Road Flemington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-9165 US00000406 A P P E N D I X A US00000407 fQUf SURV6V, INC 8I MO* 9001.06 APRIL 26, 1991 PROTOCOL ACUTE TOXICITY TEST FOR MUMMICHOO I,M IM M I HBTSR0CLIT8 (96 HOUR SCREENING) SPONSOR: STUDY NUMBER: Chubb.National, .Foam.. Inc. ...................................... .W s Sit S iliS.S.|g. jiL2.aL8.2..-- i,, J 3 1 - Q S 6 f t . . . 6 . H tf 3 A ) TESTING FACILITY: Aqua Survey, Inc. 499 Point Breeze Road Fleraington, New Jersey 08822 United States of America PROPOSED START DATE: PROPOSED COMPLETION DATE: STUDY DIRECTOR QA OFFICER: SPONSOR APPROVAL: May 2 . 199.1 ..... Mav 10. 1991 a J t ` _ Signature ____ rJ/ L eSignCat'r t11.1ul./LI ? Signatur^ .i//? / Date Date Date a ri 1 Of 6 499 Point Breeze R oad Rem ington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-91 c US00000408 6---- RQUf SURV V, INC BJBCTIVBS ' o determine the acute toxicity of the test material in the aquatic nvironment to muxnmichog under flow-through (intermittent-flow) onditions for 9 6 hours. EST ARTICLE: he test article is Aer-Q-Water 6EH _ which was received from hubb National.. Foam_ on FetproiBL.23. 1991 f the test article is traw Yellow Licmid (description). The sample is labeled with an ASI umber 91-056 A . Any test article remaining after the test is ompleted will be disposed of according to the instructions from the ponsor or returned to the sponsor. EST ARTICLE CHARACTERIZATION: ata concerning stability, uniformity, composition and additional _ hemical/physical characteristics of the test article will be provided y the sponsor on the Test Substance Form (supplied by A S I ) ._ Chemical nalyses will be the responsibility of the sponsor unless prior .rrangements have been made for ASI to assume that responsibility. I0NTR0L ARTICLES [one required. ?EST SYSTEMS ?his test will be conducted on Mummichog, Fundulus herp.sI.i&us, rUSTIFICATIONS iummichog are considered the appropriate test system as they are a :haracteristic system in which the response to toxicological agents can je evaluated. DILUTION WATERS Natural or artificial seawater will be used for this s t u d y . Natural seawater will be filtered through a filter with a pore size of less than 15 microns prior to use in the test. Artificial seawater will be prepared by adding commercially available formulations or by adding specific amounts of reagent-grade chemicals to deionized water. 2 Of 6 499 Point Breeze Road Remington, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-91 AQUA SURVV, INC. LVEMTSs enever possible, the toxicant will beintrduced into test lution Without the use oi solvents other th,a n .^ ^ ^ e used sparingly, ter. If alternative solvents are necessary, they ar P t exceeding 0 . 1 ml/L in a solution. ST MATERIALS ,e technical g r a d e , end-use product or bt?,,iiii a r e ^ u s a c ^ t o ^ e f i n e a ie brand name or chemical names and a an<| percent of each st material, mixtures are defined by ingredient and p igredient,. where possible. 3ST ORGANISMS S immichog, Fundujas heteygclilas# is p ^ t h ^ s a meP agefand be of normal ast. Fish used in ize and appearance this test for their will age. be from the The longe same age 0 . ^ and more 5 gg ian twice the length of the shortest and weigh bstwssn 'if i . i" h Will not.be used if they e x h i b i t a h,ey have been used in a previous test, eithe ontrol g r o u p . uring holding, organisms are ? d newly hatched brine shrimp rtemia salina or other appropriate food Prior^to W ^ 9ind re observed for signs of disease, s t r e s s , p y remedially ortality. Organisms exhibiting any of these signs are remea 1 .rested prior to use in testing or discarded. EXPOSURE CHAMBERS twenty (19.5) liter all glass aquariums or other appropriate size glass jr perfluorocarbon plastic chambers will be used. 3 of 6 1/1 499 Point Breeze Road Rem ington. New Jersey 08822 Telephone (908) 788-8700 FAX (908) usoo RQUfl SURVY. INC. TEST PROCEDORES: A minimum of twenty (2 0 ) fish will be exposed to one concentration of the test article (equal numbers in two replicatesj. Each test will include control (vehicle and/or untreated^ S S S a r 1the same dilution water, conditions, ' of tha chemical is f v S i S r o o ^ r t n s ' u S d r ^ o l u m e Of the vehicle will be equal to that used in the test concentration. The test article is introduced into each of the chambers. The test is started by randomly introducing fish acciimated in acSordince with the test design into the test chambers w i thin 30 minutes. . Fish in the test and control chambers are observed periodically during the test, dead fish removed and the findings recorded. The dissolved oxygen concentration, iS ^ S ^ iv^ water quality characteristics are measured at s p e c i n e a inter if" test chambers. The flow rate through each test chamber will be at least f i v e (5) additions per 24 h o u r s . ACCLIMATIONS ' If the holding water ,is not from the same source as t h u ^ i i v t i o n ^ a t e r , acclimation to the dilution water will t 9 .Y period. The fish will then be held an additional y dilution water prior to testing. organisms will temperature in not any b1e2 subjected to hour period, more ^he than g a 3 "i=s hiaher i u T be* less organisms will beaL l d /??ra m ^ i m ^ o ? f d l y f k the test temperature prior to testing. During the final 48 hours of acclimation, organisms ill in facilities with background colors and light m t e n s i those of the testing area and will not be red. TEST PARAMETERS! TEMPERATURES The test temperature will be maintained at 22 + / - 2 C . In da i l y ^ ^ i ^ a d d i t i o n ^ t h e ^ e m p e r a t u r e ^ i l l ^ b e ^ m e a s u r e d concurrently at the beginning and end of the test in all test chambers. Q / i 499 Point Breeze R oad Rem ington. New Jersey 08822 Telephone (908) 788 8 00 US00000411 DISSOLVED OXYGEN: ftQUft SURV6V, INC. ? ^ L d inSf V ? l XYI en (D0) concentration in each test chamber will be win be t t saturation at a11 t i during the te" Measurement h w r s L belwlen? beglnnln<J and end * " e test and at least every 43 pH: The pH wil1 be measured at the beginning and end o f the test. SALINITY: Salinity will be measured at the beginning and end of the test. PHOTOPERIOD: A photoperiod of 16 hours light and 8 hours transition period. with a 15 to 30 minute LOADING: s r s i r ^ s r per u ter of soiution passing OBSERVATIONS: zzsrzs srtoS ss.*-- wmbe WEIGHT AND LENGTH: s ^ L S ? * i 0dL eraluabion! ave dean performed, a random sample of 30 surviving fish are sacrificed for eight and length determination^. 5 of 6 1/1 499 Point 8reeze Road * F|sm lngton, New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-916= US00000412 RQUfi SURVV, INC REPORTING: In addition to the general reporting requirements, the reporting of test data shall include the following: 1. The source o-f the dilution water, its chemical characteristics and description of any pretreatment. 2m Detailed information about the test organisms. 3 description of the test chambers, the depth and volume of solution in the chamber, the way the test was begun, the number of organisms per treatment, the number of replicates, the loading and the lighting. 4 * The number and percentage of organisms that died or showed any other adverse effects at 24, 48, 72 and 96 hours. The age and scientific names are reported. RECORDS: All raw data, data manipulations, draft reports and final reports will be retained by Aqua Survey, Inc. Records will be surrendered to the sponsor upon their written request. QUALITY ASSURANCE: This study will be conducted in accordance with a modification of ASTM Standard Guide for Conducting Acute Toxicity Tests with fishes, macroinvertebrates and amphibians, E 729-88a. 6 of 6 499 Point Breeze Rood Remington. New Jersey 08822 Telephone (908) 788-8700 FAX (908) 788-916c US00000413 A P P E N D I B US00000414 ANALYTICAL data e m i t PACXMiC Aqua Surwf Inc. 499 Point 8raaxa ltd. PlMington, MY 08822 ATTMl REF: ji* Todd Lab Water SAMPLE IDENTIFICATION laboratory NUMBER p.O. MO. : paroma Data -.April 30, 1991 - SAMPLE MATRIX i ; ! i . ' ; S g g M 6 X T PAGE f 4 ,4f Mf CERTIFY THAT THIS REPORT IS A TRUE REPORT Of RESULTS OBTAINED P I OUR TESTS OP THIS MATERIAL. parag K. SHAH, PR. 0* ORGANIC LAB. MANAGER kb . iM e lie n i tp p # i* mi sem ew sk Rao*** 0,1 i,n nilgirt i ^ | | m |Q ga| IfltelfW lW I - - la in a rt lo r tnwty d iv e laaaaiiuH* * * ' baia a l "Sox -t518 a 60 seaview blvd.. port vvashington, ny i 1050 a (516) 625-5500 US00000415 LABORATORY NUMBER 7861001 7861002 7861003 SAMPLE IDENTIFICATION LG SALT LG FRESH LP FRESH TYPE OF SAMPLE Water Water Water US00000416 nytest environm ental* DATA REPORTING QUALIFIERS Indicates compound was analyzed for but not detected. Report the minimum detection limit for the sample with the U (e.g. 10 U ) based on necessary concentration dilution actions. (This is n ot necessarily the instrument detection limit.) The footnote should read U-Campound was analyzed for but not detected. The number is the minimum attainable detected limit for the sample. _ Indicates an estimated value. This flag is used either when estimating a concentration for tentatively identified compounds where a 1:1 response is assumed or when the mass spectral data indicates the presence of a compound tha t meets the identification criteria but the result is less than the specified detection limit but greater than zero (e.g.: If limit of detection is 10 ug/1 and a concentration of 3 ug/1 is calculated, report as 3J.) ' This flag is used when the analyte is found in the blank as well as a sample. It indicates possible/probable blank contamination and warns the data user to taka appropriate action. This flag identifies all targeted compounds that were found above the method detection limits. This flag indicates that the compounds are not applicable. Aldol condensation product. Note: Data on soil samples expressed on a dry weight basis. All non-water samples are reported .on soil forms. This includes samples whose matrix is listed as miscellaneous. The initial and continuing calibration dates and times for the volatile fraction are listed on the BFB summary f o r m s . The initial and continuing calibration dates and times for the semi-volatile fractions are listed on the DFTPP summary forms. 00003 nytest enviranm enta I REPORT O P ANALYSIS m f i n d as follows* Log In N o .: 7861 Parameter(s) pH S a lin ity , ppt R e s u l t s i n mg/Is Ammonia N i t r o g e n Bromide Fluoride Io d in e Nitrate Phosphate Residual C h l o r i n e Su lfide ` S u lfa te Total Cyanide Total S u s p e n d e d S o l i d s Sample Id e n tific a tio n LG SALT LC FRESH LP FRESH Method ( 7 8 6 1 0 0 1 ) (7861002) (7861003) Blank 7.6 6 29.8 NA <1 0.130 40.8 0 .5 7 6 <1 <0.04 <0.04 < 0.1 < 0.10 1910 < 0.01 3 0.22 < 0.1 5 < 0.1 <0.05 < 1.0 < 0.02 <1 < 0 .0 4 <0.04 < 0.1 < 0.10 <1 < 0.01 <1 ppt parts per thousand 00005 US00000418 test environm ental me. ' f in d as fo llo w s * suits i n mg/Is aram eter(a ) luminum esenic ry lliu m 3ron admium alcium iromiua sbalt spper :on . sad ignesiura mganasa rcury >lybenura ckal tassium ilenium .lve r .nc 'tal O r g a n i c C a r b o n REPORT OF ANALYSIS Log In N O .:7861 Sample I d e n t i f i c a t i o n LG SALT LG FRESH L? FRESH M e th o d (7861001) ( 7 8 6 1 0 0 2 ) (7861003) 3lank . a . * .* <0.2 < 0 .0 1 0 <0.005 3.3 <0.005 271 <0.010 <0.05 <0.025 <0.050 <0.050 932 < 0 .0 1 5 <0.0002 0.16 <0.040 348 < 0 .0 10 < 0 .0 1 0 <0.020 20.5 --- -- ** *-- -- rmmm -- -- -- TM -- -- --- -- -- -- 1 1 .3 -- < 0 .0 1 0 -- -- < 0 .0 0 5 -- -- -- < 0 .0 2 5 -- < 0 .0 5 0 -- <0.0002 -- < 0 .0 4 0 -- .. -- < 0 .0 2 0 ~ < 0 .2 < 0 .0 1 0 <0.005 < 0 .1 <0.005 < 1.0 < 0 .0 1 0 < 0 .0 5 < 0 .0 2 5 <0.050 <0.050 < 1.0 < 0 .0 1 5 < 0 .0 0 0 2 < 0 .0 1 0 < 0 .0 4 0 <1.0 < 0 .0 1 0 < 0 .0 1 0 < 0 .0 2 0 < 1.0 00006 US00000419 ' 1 O-T HfTEST EMVIRflMMWrAL IMC. TO. PESTICIOE/PCS 0RGANIC3 ANALYSIS OATA SHEET aro SAURE MATRIX: WATER SAURE IO: COMC. lEVELi LO LAB SAKRE IO: EXTRACTION OATS: 4/9/91 DIL FACTOR: ANALYSIS DATE: 4/18/91 X MOISTURE: UC/L CAS Muntar P6STICI0S/PC8 COHPCUNO 16 SALT 7861001 1.00 MA 1 I 319-84-6 2 | 319-85-7 3 | 319-86-8 j4 58-89-9 5 | 76-44-8 6 | 309-00-2 7 | 1024-57-3 8 | 959-98-8 9 | 60-57-1 10 [ 72-55-9 11 j 70-20-8 12 | 33213-65-9 13 | 72-54-8 |14 1031-07-8 15 | 50-29-3 16 | 53494-70-5 17 | 72-43-5 18 | 57-74-9 19 | 8001-35-2 20 | 12674-11-2 2 1 - 111104-28-2 22 | 11141-16-5 23 | 53469-21-9 24 | 12672-29-6 25 | 11097-69-1 26 | 11096-82-5 1 [ Alpha-aiC | Bata-BHC | Oalta-BHC j Gamm-BXC(Lindane) | Haptacilior | Aldrin I Haptachlor Epoxida | Endosulfan I | Oieldrin | 4,4-006 l Endrin | Endosulfan II | 4,4-000 | Endosulfan Sulfate | 4,4'-00T | Endrin Keton | Mtthoxychlor | Chtordan* | Toxaphana | Aroclor-1016 | Aroclor-1221 | Aroclor-1232 | Aroclor-1242 | Aroclor-1248 j Aroclor-1254 | Aroelor*1260 i 0.050 U. 0.050 U. 0.050 U. 0.050 0. 0.050 U. 0.050 U. 0.050 U. 0.050 U. 0.100 U. 0.100 U. 0.100 u. 0.100 g. 0.100 u. 0.100 u. o.ioo g. 0.100 g. 0.200 g. 0.500 g. 1.000 g. o.soo g. 0.500 g. 0.500 g. 0.500 g. o.soo g. 1.000 g. 1.000 g. 00007 US00000420 ORGANOPHOSPHOROUS PESTICIDES Log in 0. 1 7861 Sample ID No.i LG SALT Lab ID No.: 7861001 Parameters (ug/1) Detection Limit (ug/1) Vapona (Dichlorvos) Phorate Diazinon Naled (Dibron) Dementon - S Disulfoton Ronnel Chlorpyrifoa (Dursban) Bolstar (Sulprofoa) Stirophos Fansulfothion Azinphos methyl (Guthion) Coumaphos Malathion Parathion 0.2 0.2 0.6 0.2 0.2 0.2 0.3 0.3 0.2 2.0 6.0 1.5 1.5 0.3 0.3 ND - Not Detected Found ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 00008 US00000421