Document Qk1J7EKR0oKgyw2MejDNBMb37

AR226-2789 FOR DU PONT USE ONLY E. I. du Pont de Nemours and Co., Inc. Haskell Laboratory for Toxicology and Industrial Medicine Eiktofi Road, P. 0. Box 50, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 79-84 Material Tested* 1) Octanoic acid, Pentadecafluoro-, ammonium salt Haskell No. 12,037 Other Codes C^8 2) Ethanol 14.547 Pub!icker Chem. Co. Study Initiated/Completed 6/24/82-6/22/82 Material Submitted by fr^rSW ^e^i^iTitevelopm ent Haskell Laboratory ACUTE ORAL EFFECTS OF C-8 AND ETHANOL IN THE RAT Summary: Young adult male CrlrCD rats were administered a single dose of ethanol by intragast-ic intubation at 5,000 mg/kg or a 15% (v/v) solution in drinking water for 1.4 consecutive days and/or a single dose of ammonium perfluorooctanoate at dose levels of either 570, 480, or 200 mg/kg. All rats died within 8 days after dosing with ammonium perfluorooctonoate at 670 mg/kg. Liver weight/body weight ratios showed a sharp increase in all rats that received ammonium perfluorooctanoate when compared to the untreated controls and the animals that received only ethanol. There were no significant ' differences between the untreated controls arid thr; animals that received only ethanol or between the ethanol pre-treated ammonium perfluorooctanoate and the rats that received only ammonium perfluorooctanoate. Purpose: To determine if pre-trpatment with ethanol modifies the toxicity of ammonium perfluorooctanoate. Procedure: Seventy-two young adult .male Crl:CD rats were divided into 12 groups, 6 rats per group, and individually housed. Eleven-groups of rats were administered either a single dose of ethanol at 6,000 mg/kg by intragastric intubation as a 60% aqueous solution or a 15% aqueous solution (v/v) in drinking water for 14 consecutive days and/or a single dose of ammonium perfluorooctanoate as a 60% solution at dose levels of either 670, 480, or 200 mg/kg. An additional group of untreated rats served as controls. Clinical signs were recorded daily and body weights were recorded at 0, 1, 2, 3, 6, 7, 8, 10 and 14 days. Liver weights were recorded at the 14-day sacrifice. - 1- Company Sanitized. Does not contain TSCA CBI Results: Ethanol Average Bod)f Weight Dose Level 0 Day 1'1 Days 0 6,000 mg/kg 15% (v/v) 351 339 346 416 399 393 Average Liver Weight (g) 19.61 18.43 17.52 Average Liver Weight/ Body Weight Ratio (mg/kg body weight 1 4.72 4.59 4.44 Clinical Signs: Prostration on day of dosing at 6,000 mg/kg. Slight weight loss for the first 2 days of ethanol in drinking water. day by ammonium perfluorooctanoate Dose Level (mg/kg) Average Body Weiqht 0 Day 14 Days Average Liver Weight (q) Average Liver Weight/ Body Weight Ratio (mg/kg body weight) 670 330 480 329 200 335 390 _ 24.18 6.23 Clinical Si qns: Wet and stained perineal area and weakness at 200 mg/kg. All animals died within 6 days after dosing at 670 and 480 mg/kg. Single dose of ethanol at 6,000 mg/kg followed 24 1hours later with doses of ammonium perf1uorooctanoate Dose Level (mq/kq) Average Body Weiqht 0 Day 14 Days Average Liver Weight (g) Average Liver Weight/ Body Weight Ratio (mg/kg body weight) 670 343 480 343 369 200 341 389 25.83 24.99 6.97 6.41 Clinical Si(gns: All rats at 670 mg/kg died within 8 days after dosing. -- ............ - -- -- - -- -- f 'j ****/ w i - v v i n u u p l . l tested. Stained face and alopecia at 480 mg/kg. One -2 Results: (cont'd) Ammonium Perfluorooctanoate Dose Level Average Body Weight (mg/kg) 0 Pay 14 Days Average Liver Weight (g) Average Liver Weight/ Body Weight Ratio (mq/kg body weight) 670 480 200 345 353 392 374 424 23.99 30.59 ' 6-23 7.20 Clinical Signs: and 4S0 mg/lcgT Wet and stained All animals were perineal area, weakness found dead at 670 mg/kg and lethargy at 670 within 4 days after dosing and 1 died at 480 mg/kg. * H-12,037 Purity: Contaminants : Synonyms: o Ammonium Perfluorooctanoate C-8 * H-14,547 -3- not co,1nW*h t s c a cB1 Saiw^ 0eS C O W P an^ /: / Report by: 4 !le John E. Henry Supervisor, Toxicology' Approved by: JEH:sgl:1.35 Date Issued: October 8, Haskell Lab. Report No. 79-84* Number of pages in this report: 4 -R&^LJL A V Gerald L. Kennedy, J *. Section Supervisor Acute Investigations 4QOPp^i -^con'-ainT' tori.**** '