Document Qgov5N5NDY6nqRjqGM3L5pD97

M~ A C U TE TO X IC ITY TO A Q U A TIC IN VER TEB R A TES (DAPHNIA MAGNA) T E S T SU B STA N CE______________________ _ _ ____________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFO S, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (C A S # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FC-203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyitriazole. The following summary applies to a mixture With incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemical component o f the test sample. M ETHOD:_________________________________________________________ M ethod: O EC D Method 202 Typ e : Acute static G LP: No Year com pleted: 1991 Species: Daphnia magna Supplier: In-house cultures. A nalytical m onitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours T e st organism age: <24 hours old Statistical m ethods: No calculations performed, based on study results. Te st conditions: Dilution w ater: Filtered natural well water Dilution w ater chem istry: C o n d u ctivity: 840 pmhos/cm pH: 7.6 Lighting: Cool-white fluorescent lights at 20 pEin/sec/m2. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in dilution water. Aliquots were added directly to dilution water-------in test vessels to create test solutions. Exposure ve sse ls: 250 mL beakers containing 200 mL exposure solution at a water depth of approximately 6 cm. Num ber o f replicates: 4 Num ber o f daphnids per replicate: 5 001143 Num ber o f concentrations: five plus a blank control W ater chem istry during the stu d y: pH range (0-48 h o urs): 7.6 - 8.3 (control exposure) 7.6 - 8.3 (1000 mg/L exposure) Tem perature range (0-48 hours): 19.0 - 20.2 C (control exposure) 19.0 - 20.1 C (1000 mg/L exposure) C o n d u ctivity range (0-48 hours): 840 - 880 pmhos/cm (control exposure) 870 - 870 pmhos/cm (1000 mg/L exposure) D issolved oxygen range (0-48 h o urs): 8.5 - 9.2 mg/L (control exposure) 8.5 - 9.3 mg/L (1000 mg/L exposure) R E S U LTS _______________________________ _ ___________ ____________ Nom inal concentrations: Blank control, 150,250,400, 600, and 1000 mg/L. Elem ent values: 48-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C LU S IO N S ____________________________________________________ The test substance 48-hour E C 50 was determined to be >1000 mg/L, the highest concentration tested. No mortality or sublethal effects were observed at this concentration. Subm itter: 3M Company, Environmental Laboratory, P .O . Box 33331, St. Paul, Minnesota, 55133 DATA Q U A LITY _________________________ ___________________________ R eliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and it lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E FE R E N C ES _________________ ___________ ________________________ This test was conducted by EnviroSystem s Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, Lab Request number J 1884-1,1991. 001144 OTHER__________ Last changed: 6/26/00. 001145  , - - ;- & fvJefcv v.'vj'i- \ V '" '- - i? vVVv-?Ff':'>**. .. R!'.&Si*. '*%.'. ' " ' k->~\ ` : .,," -'..: V - . >V -.,.a ,V - vr-* Study Title Static Acute Toxicity of TC-203CK to the Daphnid, Papbaia maaoa *$'vs V ^ ; " 4 ]* ;^ 4 X '" |.f' ';<r.i V--i .. .. It. Author Timothy J. Ward Robert L. Boeri OrS- .. Study Completed . -V ' V.uU/*' fe-.'-f -'-'V'/-'- :`v 4'S:^-%?yV'b;- s% Performing-Laboratorv ,- %;, > f 5&S6>5, v. i*f '- .,> I \ '52,<-#**** * ` ' IF / itl - : ^ - m W ^^ U\i- ^ $ pfe's ' t v1, i`1'% *fr," , ' ''.I'*!. ' mV-'1" .1 I V . y X i '; * * -p 0011*6 BE9 fGOPY AVAILABLE The acuta toxicity of FC-203CB to the daphnid, Dapbnia mapaa, is described in this final report. The test was conducted for 3H Company for 48 hours during June 10 to 12, 1991, it the EnviroSystems Division of Resource Analysts, Inc. in Haapton, New Hampshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Tiaothy Ward. The test was perforaed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 1C. The dilution water was filtered natural well water collected at Haapton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC-203CE were: 0 ag/t (control), 150 mg/L, 250 ag/L, 400 ag/L, 600 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0001 g, resulting in a loading rate of 0.003 g/L. All daphnids were in good condition at the beginning of the study. Exposure of daphnids to the test substance resulted in a 48 hour EC50 greater than 1,000 mg/L FC-203CE. 1~ 'BEST COPY AVAILABLE xx. t i b i a or coNTBrrs r.; SECTION: 'V'*;;; >?->> ' v-. I. Suuary v^;y .:*.*/ , II. :V Table of Contents !'*;'*'` / v-V''' III. Index of Tables IV. <. Introduction i - - :. . ft f; `V ; .4'v V. Methods and Mater: JKi&: i-f;vi. Results i , * Vr' -y -]: , r` . VII.':' 'References^:'.-' >-. - . v? ; Appendix A*' ffater Quidity P e ^ froei Toxicity Test ' " : ;.-V I tj - i , IS j#''`y>i! 00114S diligi * i in . IHDEC or TABUS AMD FICOTHS v ^ .Tabl'i; ' ' ' ' *5Ci Chemical characterization of a representative sample of natural ell water used as dilution water for toxicity test - Tabliji Survival data from toxicity test Table 3. Median effective concentrations (EC50s) tUr'' *ro* toxicity test : - v ' ' . -7 , Table A.1. Conductivity, pH, teaperature, and dissolved oxygen concentration measured during toxicity l`te 't- 7C--->y-: % g ^AVAILABLE zt. introduction This study vas sponsored by 3H Company, St. Paul, Minnesota. The objective of the study vas to deteraine the acute toxicity of FC-203CE to the daphnid, a freshwater invertebrate. The report contains sections that describe the nethods and naterials enployed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. METHODS AMD MATERIALS TEST SUBSTANCE: FC-203CE (EnviroSystens Sanple tNumber 2935E) was delivered to EnviroSystens on May 23, 1991. It was contained in a 4 oz. plastic bottle that was labelled with the following 'intonation: "J1884-1, FC203CE". FC-203CE (an orange-brown liquid) was supplied by 3M Conpany, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test naterial vas stored in the dark at roon tenperature. A reserve sanple (approxinately 1.1 grans) will be archived at EnviroSystens for a nininun of 10 years. DILUTION HATER: IvVr ~ K'-?- y. Hater used for acclimation of test organisas and for all toxicity testing was collected from wells at' EnviroSystens in Haapton, Mew Hampshire. Hater vas stored in 500-gallon polyethylene tanks where it was aerated. Results of chenical analysis of a representative sanple of water are presented in Table 1. "j .TEST ORCAMISM: ; - 7-?-.- ' Juvenile daphnids enployed asjtest organisas were fron a single ^source'.and were identified using an Appropriate taxonomic key. Daphnids f ' S^usedSin the test were product .iron.;an-iA-house culture and were less than Soli at .the startfoiithe test^g^ontrol daphnids were weighed at to testing, daphnids were v'-Sv ^.'SSKisaintained^inilOOt dilution^water undeVfftatic conditions in 4 liter glass ^ l ^ ^ arsI^^DuringsyacciinatitAfdapto'idsrwerSinotltreated for disease' and they : ^^were^free|tof^Ap^rentsic&essi;^.i^uittM'^''and abnormalities.: at ? the; 24 hours"prior to the'4test^ihiWatioi^the;teni^rature,waaSl9;5cyiiDaphnids were fed yeast, Isttrroouufct'#^.>chhnoww;:3#&-'arid thr^5 f r it iiln n t * r w S i n rSellintaastrum capricornutum once af0j*4irf ^sf*4itti its v-fj; f t ; 001150 I ^ ^ ^ k -:^:- - J p f ^ :' V - . . ; - :; : ; ' - ss : iB e s f ' -Vt '^' ^ . . s . } ra, Table 1. Cheaical characterization of a representative sanple of natural well water used as dilution water for toxicity test Paraneter Unit of . Reporting Heasurenent .Lisit Measured Value pH V.---V Conductivity 1<.:.1 Organ Morine pesticides Organophosphorus pesticides pH units uahos/cn ug/L Ug/li -- \V.;0e5 /.,'.K.J-rSl'' 0.5 7.6 840 HO HD ...v-#3 .. ., w fw liS?* 4 Polychlorinated biphenyls ug/h 0.5 ` ' HD MV S .' Mote*: I. .'Hone detected at or above,r.the-reporting liait. aMi/ 2., ph and condactivit^/wer^aeasuredlin dilution water :*:collected iron controIMtestJvessel prior to A - introduction :o orffaida^in^eiiticide and PCB data is . CQllected durlng routine^>iannul.|water quality testing.,: T-i O Ai-v,-. - 4 > Sag* v>. j.' ' n.\ 'SfivU'.y>:-?Vpl.i- |I i& l! 001151 xv: rV'^'Vv BEST COPY AVAILABLE TOXICITT TESTING: The definitive toxicity test was perforsed during June 10 to 12, 1991. It is based on procedures of the OECD (1984). The test was conducted at a target teaperature of 20 1 1*C with five concentrations of test substance and a dilution water control. A 1,000 ng/L stock solution was foraulated without the use of a solvent. lfoainal concentrations of the test aaterial were: 0 ng/L (control), 150 ng/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Twenty daphnids were randonly and equally distributed aaong four replicates of each treatment: The.test was perforaed in 250 al glass beakers that contained 200 .*al of test solution (water depth was approxiaately 6 ca). Test vessels were randoaly arranged in an incubator during the 48 hour test (a randoa numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was autoaatically aaintained with cool-white fluorescent lights that provided a light intensity of 20 uEs^a-1. Aeration was not required to aaintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The nuaber of surviving organisas and the occurrence of iaaobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal 'effects (loss of equilibriua, erratic swinaing, loss of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 48 hours. Dissolved oxygen (YSI Model 57 meter; instrument number PXL-3), pH (Beckman model -pHI 12 meter; instrument nuaber PRL-51), conductivity (Cole Parmer nuaber '1481-60, instrument number PRL-50), . and: ..temperature (ASTH mercury. thermometer; thermometer number 2265) were' meaaured and recorded .daily in each test chamber that contained live'aninals. . iz' STATISTICAL METHODS: i*'"V Results of the `toxiity:;test could not be interpreted by studard ^ statistical techniquespStephan, 1983) because 50% survival or greater occurred in. alI t estedlonchtrations. U 'M 00115 ***' fJpST* ST COPY AVAILAB '> . n .;KMOWS v 1;,'J,`v -;. All test vessels containing FC-203CB were initially clear and regained clear througbout the test. Biological and water quality data generated by the acute toxicity test; are presented in Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control daphnids had an average wet weight (blotted dry) of 0.0001 g at the end of the test. Loading rate during the toxicity test was approximately 0.003 g/L.. The 24 and 48 hour BC50s for daphnids exposed to FC-203CE are presented in Table 3. The 48 hour EC50 is greater than 1,000 eg/L FC-203CK. VJi', . i%'-i`rr:. f Sr,9** rA'.. y&- 0 },?<I'-i " `-, ' $/v ...... :/-K ;.' 001153 *>;% i:' ^ '. iX Table 3. Median effective concentrations (ECSOs) fro* toxicity test Exposure period EC50 95 percent confidence li*its Calculation ethod 34 hours >1,000 mg/h 48 hours >1,000 g/h . '.v.. ' ' .SSv'C:, V -- -- B|S|teOPY AVAILABLE ' '** 'vtpfK1-'- 00115S 001156 I BEST COPY AVAILABLE v' . ' . -y / Table A.1. Conductivity, pH, teeperature, and dissolved oxygen concentration eeasured during toxicity test Conductivity (ueho/ci) pH .Noeinal concentration * ? {g/M/ Rep. 24 48 hr hr 0 24 48 hr hr hr Vfc V' { ? > ,g}- 870 880 870 880 870 880 870 880 7.6 7.6 7.6 7.6 150 1 840 860 880 7.6 2 840 860 880 7.6 3 840 860 880 7.6 4 840 860 880 7.6 250 860 880~ 7.6 860 880 7.6 860 880 7.6 860 880 7.6 ' -400" '-C %ys' 850 860 860 860 > ' * ^860 860 860 860 880 880 . 880 f* ' 880. 7.6 7.6 7.6 7.6 X i o o " 0 M M ; 1 :' I 870 7.6 870 r-' , 880- 7.6 870 ;'iit880|!<' 7.6 860 -870 m & m 870 ^870:h & m m 7.6. t ,'870 ' Jz.870j: |3^r.r'870 '|?870 "*^ ;.;8 7 o ,: r 7.6i 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 7.7 8.3 ; 7.7 --..8.3 h 7.7 I.8a3 4'r 8.3 > .w7*.73/r1 8.3.?', -X a | ftf,'=c- T.-> Hf-. m ^ 001158 .7 SiV- $ I<!?'- *. ii ,,'J i BEST COPY AVAILABLEt- ,id- -* ;. /Table 1.1. Continued. Noainal concentration ; (ng/L) Rep. Teaperature (C) 0 24 48 hr hr hr Dissolved Oxygen (eg/L) 0 24 48 hr hr hr S -*--. ,# (; 0 (control) f.' 150 AA ' 'S ' 250 S, v.\,v "* K .; ' 1 *a*. , ;?k, i '- .+.3*1 ' tol it n-J1:- I -fiAs 20.0 20.2 20.1 20.2 19.0 19.0 19.1 19.1 19.1 19.3 19.4 19.3 8.5 _8.5 8.5 8.5 9.0 9.0 9.0 9.0 9.2 9.2 9.2 9.2 19.9 19^2 195 8.6 9.0 19.8 19.0 19.4 8.6 9.0 20.0 19.2 19.3 8.6 9.0 19.9 19.1 19.3 8.6 9.0 9.3 9.3 9.2 9.2 20.0 19.8 19.9 20.0 19.3 19.0 19.1 v 19.1 19.4 19.2 19.2 19.2 8.6 ; 9.2 8.6 9.2 8.5 9.2 8.6 9.2 9.2 9.1 9.0 9.0 20.1 19.2 19.3 20.0. / 19.0 19.4 1 9 .9 * 4 19.1. 4 1 9 . 4 ' 4 V 3l9.8si&'/19.1-., -m&m, v*-(l4--*-*vM**4*.f.i,.r 19.5 v;.19.4i-.: "20121 ?;20i2k |l9:2P;^l9i 8.5 9.2 9.2 8.6 9.2 9.0 8.6 9.2 '9.0 8.6 9.2 ,9.0 8.5 9.3 9.y 7 , 9.3 9.3 7 ..rV,i -9-3 ' v..9.2:4 7 .1^913 ,T9.2^ cl9.2.,sj 19.` ^ |l9i **! !: K'^e.3 9.0 : 3 (.jlf*-8.9Cs-' 3 t.y. -# o^SS'r *41. t'H fei* 001159 mm