Document QgeXaxVpbnyOLX7mo2EJ5DO1k
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: PreefrefrlrueodrotoocatsanPeFsuOlSfAonAy,laFmCi-do1(2e8thoyr)aascetthaetem;ajmoarycoalmspoobneent of F[(Ch-e1p2t7ad,ecFaCf-i1uo2r9o,ocFtCy-l)1s0u9l,foonrylFlC--,1p09o-tXa.ssi(uGmlyscailnt,e,CNA-Set#hy2l9-9N-1-
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aRemmiaxtrukrse:of 8T6h%etteessttssuabmsptlaencies FaCn-d12184.%CCu.r-reCn;tfliunofrooramlaktyilocnairnbdoixcyaltaetseitis compounds. 3M production lot number 751
METHOD:
Method: Not noted
Type: Static acute
GLP: No
Year completed: 1977
Species: Pimephales promelas
Supplier: Not noted.
Analytical Exposure
monitoring: Temperature, period: 96-hours
pH
and
DO
Statistical methods: Probit analysis.
Test fish age: Not noted.
Length and weight: Average length = Not noted
Pratreatment: Not noted Average weight = 1.1 g
Test conditions:
Dilution Dilution
water: water
Aerated carbon-filtered chemistry:
well
water
Initial Dissolved Oxygen: hardness: Not noted
>9.0 mg/L
alkalinity: Not noted
pH: Conductivity:
Not noted Not noted
Stock andtestsolution Concentrations dosing
preparation: rate: Once
Not
noted
Stability of the test Exposure vessels:
chemical Not noted.
solutions:
Not
noted
Number of replicates: one
Number of fish per replicate: 10
Number of concentrations: five plus a negative control
0n338
Water
chemistry Dissolved
during oxygen
the study: range (0 -
96
hours):
5.7 ->9.0 5.1->9.0
mg/L mg/L
(control exposure) (180 mg/L exposure)*
PH range (24 - 96 hours):
7.67.8 75-79
(control exposure) (180 mg/L exposure)*
*in 1th8e03m2y0lLmgt/eLs.ttgersotugproduatpabgyi7ve2nhboeurcsauasnedtaoltlaldamtoartwalaistynooctcurred
collected
Test temperature (0 - 96 hours): 62 to 67F
a RESy ULTS
Nominal Element
concentrations: Bk value: 96-hour LC50
control, 32, = 100 (70 -
56, 100, 180, 142) mg/L
and
320
mg/L
All element values based on nominal concentrations.
SPtraotbiisttaincaallyEsvisalwuaastiuosneodftMoocratlacluiltayt:e LC50 value and the corresponding confidence limits.
c0 CoOnNcCiLbUsSIiOoNnSs 00000
The 100
test sample mg/L. with a
96-hour LCs for 95% confidence
fathead interval
minnow was determined of 70 - 142 mg/L.
to
be
Submitter: 3M Company, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
bD0 aAtTAAQQuUAAuLrIyTyY ~~ 0
Reliability: information
Koinimtihsechmertahnokdionlgog3.y.
ThSiasmpstluedypulraictykswdaoscunmotenptraotpieorlnyand
characterized. Test concentrations were not characterized.
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OLTaHER
Last changed: 5/18/00
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AQUATIC TOXICITY WNOGR,KLASBHOEREATTORY
=
Type Test 96 Hours STATIC
Test Organisn_Fathead Minnow
Material Tested f-/c 2g Lo7 757
Date Started 8-22-77
Avg. Weight _ yy
=
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24 hrs
survi$val pu Dp.n0
48 hrs
Survi4val pH Dp.pm0
72 hrs
survi$ve; BH Dpp.mo
96 hrs
Survi%val ph Dp.om0
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AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET
Date Started:
Material Tested: FC-\9
Time Started:
(M4) (PM) Test Organism (Fathead minnow)
Type Test: (Continuous Flow) (Static) Avg. ht. (gram) Avg. Size
(ine
Exposure Period:
Hour Date Completed:
Diluent:(carbon-filt.-St.P. City Wer.) Analysis by:
ax No. of Doses
3 |"|
observed 3|
@
Expected 3|
)
Corrected.
0-E Observed-|
Contributi to. chi Cire
Plotted | Cone. Mortality | Mortality | Values| Expected | Nomograph
1 [o] oo [ TT =
2 [seToTTT
3
Loo| co| 11
4 5
[ee| [ze]
go [TT eo[00a| oan
rr TT
7
| 11
K=_]0__Aninals/Dose =
Total contribution to chi =
Total Animals =
(chi?)= contrib. to chi x Tot. Animals =
ani? (p-.05) for (K-2)
degree of freedon (from Table 2) =
is less than
; therefore, the data are not significan
heterogenous.
95% Confidence' Limits for LCgy*
gy=- __L2Compld
Lego== seC e i mill
Wyg = 622 wuillll
Slope function (S) = LCgy/LCsy + LCo/LCg= _/ + / =%S +/47=
Total number of animals used between 16% and 84% expected effects (N)= _J0
.
acy = 77M . 0 (1.49S5 C (tron Nomograph 12) 1 do
Lsq/fLCgq = lower limit = \eo /i.az = __ 7p, of
Le X iC, = upper limit = tov X ld = [qo
Legg = 100 Vi
(95% confidence limits _70.4 to /d>
70 --1a=2 (1) cDoonnsoetcutliivset m1o0r0e% etfhfaencttsw.o consecutive 0% effects or more than two (2) Expected value for any dose should be greater than 0.01% or less than 99.99%. (3) Corrected value for each 0% or 100% effect (from Table 1). * There is a 95% chance that the true value of Ly) lies within these limits.
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