Document QgaVk4vZYnwNb106v1JERmY06

Younger Laboratories IlfockeMldi .,. PbaA4nac<Uo<fUii ... AttaUftU las CLIFF CAVE ROAD Saint Louis, Mo, go 129 PHONE) TILPEN 6-3040 Certificate 0^ AmltfM SUBDUCT - Toxicologloal Investigation Of 1 Hi)HAUL 6 2$ Monauto Sample Nuaibar 187 Monsanto Project Nuaber 1-66-162 Nov amber )th, 1966 STUDY CONDUCTED fOH Monsanto Coapaay, St. Louis, Missouri DabHlMLNTAL PROCiDURfi - A} Oral LD,0 (Rats, Mlxsd Sex_ Ths undilutsd compound wi aal* ud fsaals rats prsgue-Dawley strain albino .iftor ths approx UlUa Lethal/U6se ns determined, groups of sale and famals rats ,/ ?ed in inhAeglag^phes at inoreaents of 0.1 fractional loj intervals at tb levels dosifrasd-fo blanket the toxicity range thereby supplying datal >r oaloulation of the LD,0 which was done according to a modification ol ie sethojr >f o, J, da Baer. Observations verd^aadS ief toxic syaptcas ud the viscera of the aniaals that succuabed sera exaainsd aaorosoopleally. The data are shown in Table I. B) Skin Absorption MLD (Rabbits, Mixed Sex) The undiluted 00apound was applied in increasing doaea at lncreaenta of 0.2 fractional log Intervals to the closely clipped, lntaot skin of New Zealand white aale and female rabbits. The treated areas were, oevered with plastic stripe and the animals placed in wooden stocks for periods up to twentyLfour hours, after which tine they were assigned to individual cages. Observations were aade for toxic syaptoas ud the viscera of the aniaals that succuabed were examined aaoroscopically. The data are shown in Table XI. HONS 037908 ThU confidential report U not to be need in any form of edvetticini without written permleeiea To i Monsanto Coapany St. Louis. Missouri - Younger Laboratories Certificate at Analyst* - Pag* 2 (11/9/66; - 1-66-162 SUMMARY prcmui 6 2 5 A) Oral U>,0 (Rats, Mixed Sax) Ihs Oral LD}0 for sals and fsasls rats sas plaesd at 20,700 ailligrws par kllograa alth loaar and uppsr Units of 17,990 to 24,6)0 ailllgraas par kllograa. Ths ooapouad *aa elasssd as praotloally non*toxic by oral lngsstlon la aals and feaal* rats. b; Skin Absorption MSP (Rabbits, Mixed Sox) Tbs Mialaua Lethal Doss by Skin Absorption in sal* and fsaals rabbits sas found to b* grantor than 2000 ailllgraas par kllograa sad lass than 3160 ailllgraas par kllograa. Tbs ooapouad sas olasssd as slightly toxic by skin absorption la aals and fsaals rabbits. looms* LABORATORI IS - Monsanto Company MQNS 037909 Toi Monsanto Company St. Louis, Missouri Tounger Laboratories Certifioate of Analysis - Page ) (11/9/66) - T-66-lfiZ TABLE I THE ORAL LD50 or TYDRAVL 625* FOB BATS Saspls Tod Undiluted ilaal Mo. - Ban 1- Faaala 2- Mala 3- Faaala 4- Mala 3- Faaala 6- Mala 7- Faaala 8- Mala 9- Faaala 10- Mala 11- Faaala 12. Mala 13- Faaala 14- Mala 13- Faaala 16- Mala 17- Faaala IS- Mala 19- Faaala 20- Mala Weight -SAjs- 223 250 210 240 215 235 220 245 215 250 225 235 220 255 210 260 230 240 215 250 Dosa 12,600 12,600 12,600 12,600 12,600 15,800 15.800 15,800 15,800 15,800 20,000 20,000 20,000 20,000 20,000 25,100 25,100 25,100 25,100 25,100 Itta Survived Survived Survived Survived Survived Survived Died Survived Survived Survived Survived Survived Died Survived Died Survived Died Died Died Died DISCUSSION - Ths Orsl LD,0 fbr solo sad faaala rats ms placed at 20,700 ailllgraaa par kilogr nth lossr asd upper lints sf 17,390 to 24,630 silligrass par kilogram; lha aospouad sss olassad as practically uon-toxio by oral ingestion la aala and faaala rata. Survival tlaa ms tm to ala days. Toals symptom laoludsd loss of appstita, dlarrhm, inorsaslag aakaom. dyspnea, aad oollapaa. ' At autopsy thsra sas liver, raaal, aad pulmonary hyparmla. MOMS 037910 Tot Monaamto Coapaay St. Loula, Mlaaourl Xauagar Labaratarlaa Cartiflaata at Aaalyala Paga 4 (11/9/46) - 1-66-162 TABLE II TUX MINIMUM LLTHAL DOSX OT 'PTDHAUL 62 5' ST SKIN ABSORPTION IN RABBITS Saapla Appllad Undilutad N 1 I &Si9^J!Sd.:.S!S 1 Mala 3 - Mala 4 - Faaala $ - Mala 6 - Faaala balght -IXi- J.l 2.5 >.0 2.7 2.a 2.5 . Dosa 794 126o 2000 3160 5010 7960 *alght Chaaga 5 Daya Latar _____ Hi_____ 0.1 0.1 - 0.1 - 0.3 - 0.4 - 0.3 _______ Fata______ Survival Surrlvad Survival Dlad -- 9 Daya Clad -- 7 Daya Dlad -- 5 Daya DISCUSSION - Tha Miaiava Lathal Oaaa 67 Skin Abaorptioa la aala aat faaala rabbits aaa found ta ba grsatsr thaa 2000 allllgraas par kllagraa and laaa thaa 3160 allllgraaa par kllograa. Tha ooapouad aaa olassad aa slightly texlo bjr akia abaorptioa ta aala and faaala rabblta. Survival Uaa aaa lira to nlaa daya. Toxlo ayaptoaa lacludad aaakaaaa ia tao to thraa daya folloaad by paralyala. At autopay tha luaga aara haaorrbaglo and thara aaa llvar dlaooloratlaa. 0J^vl ia0Ns