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ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE___________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1 ,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3)
Remarks: The 3M production lot number was 628. The test sample is FC-600 (US). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole.
The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD:__________________________________________________
Method: Not noted.
Test type: Acute static (Range-finding)
GLP: No
Year Completed: 1983
Species: Pimephales promelas
Supplier: Commercial supplier in Brady, Nebraska
Analytical monitoring: Temperature, pH, DO
Exposure period: 96-hours
Statistical methods: Not applicable. No mortality at highest
concentration tested.
Test fish age: Juveniles
Average Length and weight: Length = 3.6 cm
Weight = 0.39 grams
Loading: 0.65 g/L
Pretreatment: Not noted.
Test conditions:
Dilution water: Carbon-filtered well water, St. Paul, MN.
Dilution water chemistry:
pH: 8.2
Dissolved Oxygen:
9.6 mg/L
Temperature range: 18C
Lighting: Not noted
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Stock and test solution preparation: Test solutions were created by direct weights additions. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted Exposure vessels: Wide-mouth one gallon glass jars containing 3 liters of test solution. Number of replicates: One Number offish per replicate: 5 Number of concentrations: Four plus two blank controls Water chemistry during the study:
pH range (0 - 96 hours): 7.9 - 8.2 (control exposures) 7.7 - 8.2 (1,000 mg/L exposure)
Dissolved Oxygen range (0 - 96 hours): 5.8 - 9.6 mg/L (control exposures^ 0.7 - 9.6 mg/L (1,000 mg/L exposure)
Temperature range (0 - 96 hours): 18 - 21C
RESULTS____________________________________________________
Nominal concentrations: 2 blank controls, 1,10, 100, and 1,000 mg/L Element value: 96-hour LC5o = >1 >000 mg/L
Element value based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS_______________________________________________
The FC-600 96-hour LC50for Pimephales promelas was determined to be >1,000 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY_______________________________________________
Reliability: Klimisch ranking 2. The study meets all the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions.
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REFERENCES This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 8875,1983. OTHER_________________________________________________ Last changed: 6/28/00
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ENVIRONMENTAL LABORATORY
AQUATIC TOXICITY DATA SHEET
EXPLORATORY (RANGE-FINDING TEST)
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